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CN-121970831-A - Application of chickpea sprout extract A in ruminant

CN121970831ACN 121970831 ACN121970831 ACN 121970831ACN-121970831-A

Abstract

The invention relates to the technical field of ruminant nutrition, and provides application of chickpea sprout extract A on ruminant, which comprises the chickpea sprout extract A as an active ingredient, wherein the chickpea sprout extract A is 0.01-0.5 part by weight, the feed additive also comprises a pharmaceutically or feedstuffs acceptable carrier, the chickpea sprout extract A is a natural extract extracted from chickpea sprouts, the natural extract is extracted by drying and crushing the chickpea sprouts, then carrying out ultrasonic auxiliary extraction by using 60-80% ethanol solution at 50-70 ℃, concentrating and purifying the extract by column chromatography to obtain the chickpea sprout extract A, wherein the chickpea sprout extract A content is not less than 80%, the chickpea sprout extract A is purified by a recrystallization method, and the carrier is one or more of corn flour, bean pulp, stone powder and premix. Solves the problems of easy degradation, low bioavailability and low utilization rate of active ingredients of the prior chickpea sprout essence A in the rumen, and can bring better use prospect.

Inventors

  • WANG CHUNYUAN
  • MA CHUANGCHUANG
  • Peng Yeli
  • MA GUANGZHI
  • FAN SHIYUN

Assignees

  • 安徽万合佳尔生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260331

Claims (10)

  1. 1. The application of the chickpea sprout essence A on ruminants is characterized by comprising the chickpea sprout essence A as an active ingredient, wherein the content of the chickpea sprout essence A is 0.01-0.5 weight part, and the feed additive also comprises a pharmaceutically or feedably acceptable carrier, and the chickpea sprout essence A is a natural extract extracted from chickpea sprouts; The natural extract is obtained by drying and pulverizing Cinnamomum zeylanicum, extracting with 60-80% ethanol solution under ultrasonic assistance at 50-70deg.C, concentrating the extractive solution, and purifying by column chromatography to obtain Cinnamomum zeylanicum extract A with Cinnamomum zeylanicum content of not less than 80%.
  2. 2. The use of biochanin a on ruminants according to claim 1, characterized in that biochanin a is purified by recrystallization.
  3. 3. The use of claim 1 for the ruminant of biochanin a, wherein the carrier is selected from one or more of corn flour, soybean meal, stone dust, premix.
  4. 4. Use of chickpea sprout extract a according to any of the claims 1-3 for ruminant, characterized in that the feed additive further comprises soy isoflavones, the weight ratio of chickpea sprout extract a to soy isoflavones being 1:0.5-1:2.
  5. 5. The use of biochanin a on ruminants according to claim 4, wherein the feed additive is a microcapsule coating formulation, the coating material is a gelatin-gum arabic composite wall material, and the coating rate is 85% -95%.
  6. 6. The use of claim 5 for the ruminant of biochanin a, wherein the ruminant is a lactation cow and the feed additive is added to the ration in an amount of 0.02% -0.08%.
  7. 7. Use of biochanin a on ruminants according to claim 6, characterized in that biochanin a is in the preparation of a feed additive for improving ruminant production performance, comprising either of the following two formulas: the formula I comprises 0.05 part by weight of chickpea sprout extract A, 95 parts by weight of corn meal carrier and 4.95 parts by weight of premix; formula II comprises 0.1 part by weight of chickpea sprout extract A, 0.1 part by weight of soybean isoflavone and 99.8 parts by weight of soybean meal carrier.
  8. 8. The use of biochanin a on ruminants according to claim 7, wherein the improved production performance comprises at least one of an improved daily gain, an improved feed conversion rate, an improved milk yield, an improved milk quality.
  9. 9. The use of biochanin a on ruminants according to claim 8, characterized in that it enables to increase the milk yield of cows in lactation by 5% -12% and the milk protein content by 0.1-0.3% points.
  10. 10. The use of claim 9 for ruminants with respect to biochanin a, wherein an effective amount of biochanin a is fed to the ruminant, said amount of biochanin a fed being adjusted according to at least one of the physiological stage, weight, productivity of the ruminant.

Description

Application of chickpea sprout extract A in ruminant Technical Field The invention relates to the technical field of ruminant nutrition, in particular to application of chickpea sprout A to ruminant. Background Ruminants (e.g. cows, beef cattle, sheep, etc.) are an important component of the animal industry, whose productivity is directly related to the economic benefits of farming and the supply of animal products. With the development of modern intensive breeding, ruminants face a plurality of production challenges, namely, on one hand, high-density breeding leads to increased animal stress and reduced production performance, and on the other hand, the requirements of consumers on the quality and safety of animal products are continuously improved, and the use of traditional antibiotics and hormone additives is strictly limited. Under the background, the development of safe, efficient and green feed additives is an important direction for ruminant nutrition research; The plant extract has the characteristics of nature, safety, no residue and the like, and has wide application prospect in ruminant nutrition regulation and control. Among them, phytoestrogens (such as soybean isoflavone and formononetin) have been attracting attention because they have estrogenic activity, and can regulate endocrine metabolism in animals and improve productivity. Researches show that the proper amount of phytoestrogens can improve the feed intake of ruminants, promote the digestion and absorption of nutrients, improve the rumen fermentation environment, and further improve the daily gain, milk yield and milk quality. Chickpea sprout extract A (Biochanin A) is a naturally occurring isoflavone compound, and is mainly derived from leguminous plants such as chickpea, red clover, and the like. In the fields of human nutrition and medicine, biochanin A is widely studied for its biological activities such as antioxidation, anti-inflammatory, anti-tumor, etc. However, in the field of ruminant nutrition, relatively few studies on biochanin A have been focused mainly on 1) metabolic characteristics studies in which biochanin A can be metabolically converted into other isoflavone derivatives such as genistein, daidzein, etc. in the rumen of ruminants. Different metabolites have different biological activities, but the effect of specific metabolic pathways and products on animal physiology is not completely understood. 2) The physiological function exploration shows that the chickpea sprout extract A can influence the nutrition metabolism of ruminants through regulating the rumen microbiology, improving fiber degradation, reducing methane emission and other ways through a few in vitro researches. However, in vivo test data are deficient, and especially, the influence on key indexes such as production performance, milk quality and the like is lack of systematic research. Despite the potential of application of plant extracts in ruminant nutrition, the prior art has the disadvantages of 1) inadequate research depth, less systematic research on chickpea sprout A in ruminants, and lack of a complete technical system from extraction purification, preparation technology to application effect. 2) The application effect is unstable, and the chickpea sprout A is easy to degrade in the rumen, so that the direct adding effect is poor, and the prior art lacks effective protection measures. 3) The dose effect is ambiguous, and the proper addition amount under different physiological stages and different production targets lacks scientific basis, so that the actual application effect has large difference. 4) The compounding technology lacks that the synergetic effect of the chickpea sprout extract A and other active ingredients (such as soybean isoflavone) is not researched sufficiently, and the nutrition regulation potential of the chickpea sprout extract A is not fully exerted. The prior chickpea sprout essence A is easy to be degraded in rumen, has low bioavailability and low utilization rate of active ingredients, thus, the application of chickpea sprout essence A on ruminant is proposed. Disclosure of Invention The invention provides application of chickpea sprout essence A on ruminant, and solves the problems that the prior chickpea sprout essence A in the background art is easy to degrade in rumen, low in bioavailability and low in active ingredient utilization rate. The technical scheme of the invention is as follows: regarding the use of biochanin A on ruminants, comprising biochanin A as an active ingredient, wherein the biochanin A is contained in an amount of 0.01-0.5 parts by weight, and the feed additive further comprises a pharmaceutically or feedably acceptable carrier, wherein the biochanin A is a natural extract extracted from biochanin; The natural extract is obtained by drying and pulverizing Cinnamomum zeylanicum, extracting with 60-80% ethanol solution under ultrasonic assistance at 50-70deg.C, concentrating the extractive solut