CN-121970832-A - Application of carp egg lectin rCcFEL2 in preparation of carp breeding feed additive

Abstract

The invention discloses an application of carp egg lectin rCcFEL to preparation of a feed additive for carp culture, wherein the carp egg lectin rCcFEL has double effects in improving disease resistance and growth performance of carps. The antibacterial function and the growth regulating effect of the carp egg lectin rCcFEL are systematically clarified for the first time, and the research blank of the lectin in aquatic antibacterial and immunity enhancing application is filled. In-vitro bacteriostasis experiments prove that the carp egg lectin rCcFEL has remarkable bacteriostasis activity on various common gram-negative bacteria and positive bacteria of aquatic products, has wide bacteriostasis spectrum and stable action effect, and further shows that the in-vivo toxicity attack experiment further proves that the carp egg lectin rCcFEL can remarkably enhance the disease resistance and defense capacity of a carp body and an ovary, effectively reduce the bacterial load in immune tissues after pathogen infection, and can actively regulate and control the growth performance of the carp and promote the healthy growth of the body.

Inventors

• XIA XIAOHUA
• LIN YANXIA
• LI PENGFEI
• Huo Weiran

Assignees

• 河南师范大学

Dates

Publication Date

20260505

Application Date

20260224

Claims (8)

1. 1. The application of the carp egg lectin rCcFEL2 in preparing a carp breeding feed additive is provided, wherein the gene sequence of the carp egg lectin rCcFEL2 is shown in a sequence table SEQ ID NO. 1.
2. 2. The use according to claim 1, wherein the amino acid sequence of the carp egg lectin rCcFEL is shown in the sequence table SEQ ID NO. 2.
3. 3. The application of the cyprinus carpio egg lectin rCcFEL as a cyprinus carpio breeding feed additive, which is characterized in that the addition amount of the cyprinus carpio egg lectin rCcFEL as a cyprinus carpio breeding feed additive in cyprinus carpio breeding feed is 40-80 mg/kg.
4. 4. The use of carp egg lectin rCcFEL2 according to claim 1 for the preparation of a bacteriostatic feed additive for carp farming, wherein the carp egg lectin rCcFEL has an inhibitory effect on both gram-negative and gram-positive bacteria.
5. 5. Use of carp egg lectin rCcFEL2 according to claim 1 for the preparation of a feed additive for carp farming in which aeromonas hydrophila biofilm is inhibited.
6. 6. Use of carp egg lectin rCcFEL2 according to claim 1 in the preparation of a feed additive for raising carp disease resistance.
7. 7. Use of carp egg lectin rCcFEL2 according to claim 1 in the preparation of a feed additive for raising carp, for enhancing the reproductive endocrine function of carp.
8. 8. Use of a carp egg lectin rCcFEL2 according to claim 1 in the preparation of a carp farming feed additive for improving the growth properties of carp.

Description

Application of carp egg lectin rCcFEL2 in preparation of carp breeding feed additive Technical Field The invention belongs to the technical field of aquaculture feed additives, relates to a new application of carp egg lectin in preparing aquaculture feed additives, and in particular relates to an application of carp egg lectin rCcFEL in preparing carp feed additives. Background Carp (Cyprinus carpio) is a worldwide important aquaculture species and is popular with consumers because of delicious meat and rich nutrition. However, with the popularization of intensive and high-density cultivation modes, the problems of deterioration of cultivation environment, acceleration of pathogen transmission and the like are increasingly prominent, and diseases such as bacteria, fungi, parasites and the like are frequently generated. Pathogenic infection can not only remarkably inhibit the growth and development rate of carps and reduce the culture yield, but also directly damage the structure and the function of a reproductive system of the carps, interfere gametogenesis and fertilization functions, and destroy the steady state of a fish self reproduction-immunity regulation network, so that the germplasm breeding efficiency of the carps is reduced, the germplasm resource quality is degraded, and serious threat is formed to the fine breed cultivation and the industrial upgrading of the carps. In current breeding, antibiotics are often used for preventing and controlling bacterial diseases, but long-term improper use is easy to cause drug residues and drug resistance, so that the health of carps is endangered, and the food safety risk is also aggravated. Therefore, the development of a safe, efficient, environment-friendly and environment-friendly green prevention and control technology capable of replacing antibiotics solves the problem of diseases by strengthening the disease resistance of carps, becomes a key technical bottleneck for promoting the high-quality development of the carp breeding industry, and has urgent industrial requirements. The innate immune system is the main defense system of aquatic animals, and the central initiation core is pathogen recognition. Lectin, as a key member of Pattern Recognition Receptor (PRRs), can specifically recognize pathogen-related molecular patterns such as bacterial Lipopolysaccharide (LPS), peptidoglycan (PGN) and the like, and initiates immune response by binding to microbial saccharides through a saccharide recognition domain. In addition, the lectin also has the functions of promoting microbial agglutination, enhancing phagocytic encapsulation of blood cells, resisting virus and bacteria and regulating, has strong thermal stability, can resist the high temperature of feed pelleting, and is a candidate material of a green aquatic feed additive with great potential. The fish egg lectin is a specific maternal immune activity molecule of fish, has antibacterial and immune regulation functions, can be transferred to offspring through parent reproduction, builds a passive immune barrier in the early development stage of the young fish, effectively resists pathogen attack, and has important significance in guaranteeing the survival rate of the young fish and improving the early development quality. Patent literature of CN200910066648.8 discloses bullfrog lectin genes, lectin coded by the same and application of the bullfrog lectin genes, wherein the bullfrog lectin genes are selected from a constructed bullfrog skin cDNA library, and the obtained bullfrog lectin can be used as functional molecules with guiding function in antibacterial feed additives, drug development and drug targeting delivery carriers. Patent literature of CN200610035461.8 discloses a sequence of a salver lectin gene, a primer for combining is designed by a conserved region of a CDS sequence of a lectin homologous gene of a near-source species, total RNA is extracted from spleen of the salver, the total RNA is amplified by a PCR method, and the total RNA is cloned to a complete expression sequence of the salver lectin gene by combining with RACE technology. The gene not only lays a foundation for researching the effect of fish lectin in inflammatory reaction and further discussing the occurrence mechanism of fish inflammatory reaction, but also can obtain purified protein with biological activity through gene sequence and provides a theoretical foundation for researching novel disease-resistant immune adjuvant. Patent literature of CN2015123083. X discloses application of lectin modified Engraulis japonicus antibacterial peptide liposome in inhibiting Listeria monocytogenes and biofilm thereof, the lectin modified Engraulis japonicus antibacterial peptide liposome is prepared according to specific affinity of phytolectin and sugar units in extracellular polysaccharide matrix of the capsule, a biological film model of Listeria monocytogenes is established, and inhibition of the lectin modified Engraulis jap