CN-121970833-A - Preparation and application of skeleton type vomitoxin antidote

CN121970833ACN 121970833 ACN121970833 ACN 121970833ACN-121970833-A

Abstract

The invention discloses a preparation and application of a skeleton vomitoxin antidote, which relates to the technical field of feed processing and has the technical scheme that: the antidote is prepared from 60% -70% of Na 2 S 2 O 5 and 30% -40% of composite carrier by a hot-melt extruder under the conditions that the sleeve outlet temperature is 60 ℃ -80 ℃ and the screw rotating speed is 30 r/min-50 r/min. The antidote is used for removing vomitoxin from the mould corn, the use effect of the framework type vomitoxin antidote is optimal when the adding level of the framework type SMBS of the active ingredient is 0.1%, and the use effect of the framework type vomitoxin antidote is optimal when the adding level of the framework type SMBS of the active ingredient is 0.4% when DON is removed from the corn alcohol lees polluted by the mould toxin. Provides a theoretical basis for further development of corn and corn distillers' grains in the field of feed processing.

Inventors

SU JUN
WANG LI
SU NING
ZHOU JIAQUAN
SU HAN
YE SONG
WANG DEHONG

Assignees

西南大学

Dates

Publication Date: 20260505
Application Date: 20251222

Claims (7)

1. The framework type vomitoxin antidote is characterized by comprising 60% -70% of Na 2 S 2 O 5 and 30% -40% of a composite carrier, wherein the composite carrier consists of 2% of 2-hydroxy-1, 2, 3-tricarballyl ester and 98% of Eudragit L100; The active ingredient in the framework type vomitoxin antidote is framework type Na 2 S 2 O 5 ; The skeleton vomitoxin antidote is prepared by screening through an L 9 （3 4 ) orthogonal test under the conditions that the sleeve outlet temperature is 60-80 ℃ and the screw rotating speed is 30-50 r/min by a hot-melt extruder, wherein the dissolution rate under the gastric environment condition is less than or equal to 20% and the dissolution rate under the intestinal environment condition is more than or equal to 70% in the orthogonal test.
2. Use of a framework-type vomitoxin antidote according to claim 1 in corn-related feed processing.
3. The method of claim 2, wherein the antidote is used to remove vomitoxin from corn.
4. The use according to claim 3, wherein the use of the framework type vomitoxin antidote is best when the added level of the framework type Na 2 S 2 O 5 as the active ingredient of the framework type vomitoxin antidote is 0.1%.
5. The use of a framework type vomitoxin antidote according to claim 1 in the processing of corn distillers' grains-related feed.
6. The method of claim 5, wherein the antidote is used to remove vomitoxin from corn distillers grains contaminated with mycotoxin.
7. The use of the framework type vomitoxin antidote according to claim 6, wherein the use effect of the framework type vomitoxin antidote is optimal when the addition level of the framework type vomitoxin antidote active ingredient framework type Na 2 S 2 O 5 is 0.4%.

Description

Preparation and application of skeleton type vomitoxin antidote Technical Field The invention relates to the technical field of feed processing, in particular to preparation and application of a skeleton type vomitoxin antidote. Background Deoxynivalenol (Deoxynivalenol, DON), an alias vomitoxin, is a toxic and harmful secondary metabolite produced by fungi, and is mainly derived from moldy feed raw materials such as corn and processing byproducts thereof, wheat and processing byproducts thereof, and the like. When mammals ingest feeds contaminated by DON, symptoms such as reduced feed intake, refusal of food and even vomiting are caused, so vomit called vomitoxin still generates toxicity due to mycotoxin interaction and the like even at low dosage of DON (1 mg/kg), and the health and production of livestock are affected. Among mammalian species, swine is considered to be one of the most sensitive animals to DON. Previous studies have reported that DON has been studied extensively for its effects on the physiological health of pigs and that DON is known to act by inhibiting cellular protein and nucleic acid synthesis, inducing cell death, etc. Zhang Lei and other researches show that the ingestion of DON with a certain concentration can cause intestinal inflammation and cell necrosis, and the DON can destroy intestinal mucosa epithelial cell barriers, so that the absorption capacity of intestinal tracts to nutrient substances is influenced, and the overall nutritional condition and growth performance of pigs are further influenced. Khaled Ghareeb et al found that DON induced changes in porcine intestinal structure, which in turn caused a disturbance in intestinal nutrient intake. Therefore, when pigs ingest feeds polluted by high-concentration DON, the pig digestion system is seriously damaged by light weight, and even the pigs die due to heavy weight, so that the economic benefit of farmers is seriously influenced. However, in practical livestock production, DON pollution is easily caused due to factors such as temperature, humidity, culture medium and the like. Taking corn as an example, corn is often mildewed when stored at normal temperature, causing a great deal of fungi to grow and reproduce, further generating DON, and finally affecting the quality of corn. Therefore, under the large background of serious shortage of feed resources in China, the feed grain resources are saved to the greatest extent, and the elimination or reduction of the toxicity of DON in the feed raw materials is important for reducing the pig raising cost and improving the efficiency. Corn is the main raw material of the alcohol fermentation industry, and China has become the third largest alcohol producing country at present, and the annual alcohol yield exceeds 900 ten thousand t. The byproducts remained in the process of preparing ethanol by corn fermentation are dried and then called dry corn distillers dried grains (DISTILLERS DRIED GRAINS WITH Solubes, DDGS). The nutrition components of the DDGS feed comprise crude protein, crude fat, neutral washing fiber, acidic washing fiber, mineral elements such as calcium, phosphorus and the like, and the DDGS feed is also rich in B vitamins and vitamin E, and is blended with the nutrition components and active factors of saccharified yeast and yeast. Wherein the content of crude protein is 26.7-32.9%, the content of crude fat is 8.8-12.4%, and the content of neutral washing fiber is 33-40%. In addition, the DDGS has very low lignin content, high protein content, high available phosphorus content, rich vitamins, low starch content and the like, and is a protein feed raw material with rich nutrition. In practical application, the DDGS is used as a main component of high-protein feed, is widely applied to mixed feed of livestock and poultry and aquatic products, and can replace traditional protein sources such as bean pulp, fish meal and the like. It is particularly popular in the rearing of ruminants, as they can directly digest such feeds. The yield of DDGS is quite considerable, and about 0.85-1 ton of DDGS can be produced per 1 ton of fuel ethanol. However, according to the report of the pollution condition of the Chinese feed and mycotoxin as a raw material in 2023, the overproof rate of DON in DDGS is 88.2%, the average content of toxin is 1.36mg/kg, the minimum content is 0.85mg/kg, and the maximum content is 1.72mg/kg, which belongs to moderate pollution, and lacks a relatively effective and economic treatment means, thus preventing the application of DDGS in practical production. How to reduce DON pollution exceeding rate while utilizing DDGS has important research significance. Vomitoxin can be detoxified by a variety of methods, physical, chemical, biological, and the like. Na 2S2O5 (Sodium Metabisulfite, SMBS) can react with two double bonds of C-9 and C-10 in vomitoxin structure, and the toxicity is eliminated by changing the chemical structure, and the growth performance a