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CN-121970853-A - Method for testing influence of artificial feed on growth and development of tomato leaf miner

CN121970853ACN 121970853 ACN121970853 ACN 121970853ACN-121970853-A

Abstract

The invention belongs to the technical field of agriculture, and provides an artificial feed and a test method for the influence of the artificial feed on the growth and development of tomato leaf miner. The fingerling is adopted to split feed, 3-head larvae per tube are inoculated, the larvae are fed at 25+/-1 ℃, and indexes such as larval stage, survival rate, pupation rate, emergence rate and the like are measured. The result shows that the difference between the formula I and the natural foodstuff is minimum, the larva survival rate, pupation rate, pupal weight, eclosion rate and hatching rate of the formula I are not obviously different from those of natural tomato leaf feeding, and the larva and pupal calendar period is prolonged, the single female spawning amount is reduced, and the formula I can still support the tomato leaf miner to complete the complete life history. The invention realizes indoor stable and physiological state-consistent insect test and provides reliable insect source guarantee for biological research and prevention and control technology development of tomato leaf miner.

Inventors

  • REN JIE
  • LI XIAOWEI

Assignees

  • 塔里木大学

Dates

Publication Date
20260505
Application Date
20260305

Claims (10)

  1. 1. An artificial feed is characterized in that the formula of the artificial feed consists of a first component, a second component and an agar solution; the first component is a mixed solution of at least one of soybean powder 15 g, cellulose 1.5 g, germ powder 9 g, soybean protein 6 g, sucrose 12 g, choline chloride 0.28 g, welch salt 3 g, casein, beer yeast 10.5 g and purified water 100 mL; The second component is at least one of corn flour 15 g, anti-chemosanguinic acid 1.2 g, sorbic acid 0.36 g, methyl parahydroxybenzoate 0.6 g, tetracycline 0.01 g, vitamin solution a 3 mL, formaldehyde solution 0.4-0.5 mL, multivitamin b 2.4.4 g, white kidney bean powder 2.4 g, cholesterol 0.1 g, raw linseed oil 1 mL or tomato leaf juice c mL; the agar solution is a mixed solution of 3-4 g of agar and 70-100 mL of purified water; The artificial feed comprises three formulas, namely a formula one, a formula two and a formula three.
  2. 2. The artificial feed according to claim 1, wherein the formaldehyde solution is 37% formaldehyde solution, the vitamin solution a is a mixture of vitamin in the 0.01 g paradigm and 10g water, the multivitamin b is a mixture of nicotinamide 2086.4 mg, folic acid 524.8 mg, VB 1.8 mg, biotin 51.2 mg, VB2 1049.6 mg, calcium pantothenate 2086.4 mg, VB6 524.8 mg, webster salt 1216 mg, VB 12.8 mg, and the tomato leaf juice c is obtained by mixing and grinding 10g of tomato leaves with 100mL water.
  3. 3. The artificial feed of claim 1, wherein: The first component of the formula I comprises soybean powder 15 g, cellulose 1.5 g, germ powder 9 g, soybean protein 6g, sucrose 12 g, choline chloride 0.28 g, welch salt 3g, casein, beer yeast 10.5 g and purified water 100mL, the second component comprises anti-chemosanguinic acid 1.2 g, sorbic acid 0.36 g, methyl p-hydroxybenzoate 0.6 g, tetracycline 0.01 g, vitamin solution a 3 mL and formaldehyde solution 0.4-0.5 mL, and the agar solution is a mixed solution obtained by agar 3.5 g and purified water 80 mL; The first component of the formula II comprises soybean powder 15 g, cellulose 1.5 g, germ powder 9 g, soybean protein 6 g, sucrose 12 g, choline chloride 0.28 g, welch salt 3g, casein 10.5 g, beer yeast 7.5 g and purified water 100mL, the second component comprises corn powder 15 g, anti-chemosanguinic acid 1.2 g, sorbic acid 0.36 g, methyl p-hydroxybenzoate 0.6 g, tetracycline 0.01 g, formaldehyde solution 0.5 mL, multivitamin b 2.4.4 g, white kidney bean powder 2.4 g, cholesterol 0.1 g and raw linseed oil 1 mL, and the agar solution is a mixed solution obtained by agar 4 g and purified water 100 mL; The first component of the formula III comprises soybean powder 15 g, cellulose 1.5 g, germ powder 9g, soybean protein 6g, sucrose 12 g, choline chloride 0.28 g, welch salt 3g, casein 10.5 g, beer yeast 7.5 g and purified water 100 mL, the second component comprises corn powder 15 g, anti-chemotic acid 1.2 g, sorbic acid 0.36 g, methyl p-hydroxybenzoate 0.6 g, tetracycline 0.01 g, formaldehyde solution 0.5 mL, multivitamin b 2.4.4 g, white kidney bean powder 2.4 g, cholesterol 0.1 g, raw linseed oil 1 mL, tomato leaf juice c mL, and the agar solution is a mixture of agar 4 g and purified water 70 mL.
  4. 4. The artificial feed according to claim 1, wherein the artificial feed is stored at a low temperature of 2-6 ℃.
  5. 5. The method for preparing artificial feed according to claim 1, comprising the steps of: S1, sterilizing, namely respectively placing the prepared first component and the agar solution in different beakers, sealing, performing damp-heat sterilization for 30 min, sterilizing at 120 ℃, and cooling to 60 ℃ after sterilization; S2, mixing, namely mixing the cooled first component with the agar solution to obtain a first mixed solution, directly adding or mixing the second component and then adding the second component into the first mixed solution, and stirring to obtain the artificial feed.
  6. 6. The application of the artificial feed in preparing products affecting the growth and development of the tomato leaf miner.
  7. 7. The method of claim 6, wherein the artificial feed has effects on tomato leaf miner in prolonging larval stage, reducing larval survival rate, reducing pupation rate, prolonging pupation stage, and reducing emergence rate.
  8. 8. The method for improving the growth and development of the tomato leaf miner according to claim 6, wherein the method for influencing the growth and development of the tomato leaf miner by the artificial feed comprises the following steps of: (1) Feed pretreatment, namely standing the artificial feed in a climatic chamber for half an hour and then dividing the artificial feed into standard feed blocks; (2) Inoculating the larva of the tomato leaf miner to be tested into a container containing the feed block, wherein the inoculating density is 2-5 heads per container; (3) Raising, namely raising under the condition of 25+/-1 ℃.
  9. 9. The method of claim 8, wherein the standardized feed in step (1) is a cylindrical feed having a diameter of 1.5 cm obtained by pressure-dividing the vial openings, and is dispensed into sterilized vials.
  10. 10. The method of claim 8, wherein the seed density in step (2) is 3 larvae per finger, and the tomato leaf miner larvae are placed about 1cm from the feed block.

Description

Method for testing influence of artificial feed on growth and development of tomato leaf miner Technical Field The invention belongs to the technical field of agriculture, relates to artificial feeding of pests, and in particular relates to a test method of influence of artificial feed on growth and development of tomato leaf miner. Background Tomato leaf miner (Tuta absoluta (Meyrick)) belongs to Lepidoptera (Lepidoptera) and is a family of moths (GELECHIIDAE), and since the first discovery and naming of tomato leaf miner in Peruvian 1917, has been widely spread in a plurality of tomato main producing areas worldwide, becoming an important invasive pest threatening the tomato industry. Tomato is used as a vegetable crop with leading yield and benefit in China, and the industrial safety of the tomato is seriously threatened by the insect. In 2017, 8 months, the insect is found for the first time in the Xinjiang Yi plow area of China, and the main larva stage damages caused by eating leaves, tender stems and fruits, and is spread to most tomato planting areas in China at present, so that continuous pressure is formed for production. The development of the insect comprises four stages of eggs, larvae, pupae and adults. The eggs are primarily milky white and gradually changed into orange, and are mostly scattered in leaves and stems. The larvae belong to chewing mouthparts, the total age is four, the body color gradually changes from creamy yellow to white to green along with the age, and part of the individual backs have red stripes and the body internode constriction is obvious. The pupa is a pupa, is cylindrical and gradually changes the color from emerald to brown. The adult body is light gray to grey brown, and the tail end of the abdomen is provided with a horseshoe-shaped short tuft. Because the eggs and the low-age larvae have tiny volumes and hidden harm, and the adults can lay eggs at the calyx to cause the larvae to directly invade the fruits, the insects are easy to spread along with the dispatching of the seedlings and the fruits in a long distance, and the prevention and control difficulty is remarkably increased. At present, researches on tomato leaf miner are focused on biological characteristics, morphological identification, field control technology and the like, and researches on standardized artificial feed suitable for the tomato leaf miner and matched feeding technology are relatively lacking. In the aspect of experimental insect acquisition, a large amount of tomato plants and space are consumed by depending on host plant feeding, the period is long, the field acquisition is limited by seasons and regions, and a sufficient amount of experimental insects are difficult to stably supply. In addition, the insects have the characteristics of generation overlapping, strong fertility and the like, and the control relies on chemical means, but the drug-resistant population appears due to long-term single administration, so that a large number of healthy and physiological-state uniform insects test are required for developing novel green prevention and control agents, developing drug-resistant management, screening mixed agents and other researches. Therefore, the artificial feed is prepared, indoor artificial feeding is carried out, reliable insect source guarantee can be provided for biological, ecological and toxicological researches on the tomato leaf miner, key technical support can be provided for resistance monitoring, medicament screening and comprehensive treatment strategy formulation, and the method has important scientific research value and application prospect. Disclosure of Invention The invention aims to provide a test method for the influence of artificial feed on the growth and development of tomato leaf miner, and solves the problems of unstable insect source, high cost, long period and large individual difference caused by depending on host plant feeding in the prior art by controlling the feed formula. In order to achieve the aim, the invention provides the artificial feed, wherein the formula of the artificial feed consists of a first component, a second component and an agar solution; the first component is a mixed solution of at least one of soybean powder 15 g, cellulose 1.5 g, germ powder 9 g, soybean protein 6 g, sucrose 12 g, choline chloride 0.28 g, welch salt 3 g, casein, beer yeast 10.5 g and purified water 100 mL; The second component is at least one of corn flour 15 g, anti-chemosanguinic acid 1.2 g, sorbic acid 0.36 g, methyl parahydroxybenzoate 0.6 g, tetracycline 0.01 g, vitamin solution a 3 mL, formaldehyde solution 0.4-0.5 mL, multivitamin b 2.4.4 g, white kidney bean powder 2.4 g, cholesterol 0.1 g, raw linseed oil 1 mL or tomato leaf juice c mL; the agar solution is a mixed solution of 3-4 g of agar and 70-100 mL of purified water; The artificial feed comprises three formulas, namely a formula one, a formula two and a formula three. Preferably, the formal