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CN-121970856-A - Preparation method of intestinal targeting type high-absorption ganoderma lucidum composite fermented beverage

CN121970856ACN 121970856 ACN121970856 ACN 121970856ACN-121970856-A

Abstract

The invention discloses a preparation method of an intestinal targeting type high-absorption ganoderma lucidum composite fermented beverage, and belongs to the technical field of functional food processing. The method comprises the steps of separating a supernatant and mycelia from ganoderma lucidum fermentation liquid, extracting ganoderma lucidum polysaccharide from the mycelia, carrying out enzymatic recombination with prebiotic oligosaccharide under the catalysis of transglycosidase to form a polysaccharide-prebiotic complex, loading triterpene compounds, taking the supernatant and tea polyphenol to form a complex core, coating chitosan and citrus pectin by electrostatic layer-by-layer self-assembly to construct pH responsive nanoparticles, and finally mixing the complex system, the nanoparticles and auxiliary materials, sterilizing and filling to obtain the product. The invention realizes the targeted release and high-efficiency absorption of active ingredients in intestinal tracts through the synergistic effect of enzymatic recombination and nano assembly, remarkably improves the bioavailability and the product stability, and is suitable for the preparation of functional foods for regulating intestinal flora and enhancing immunity.

Inventors

  • LIU SHIZHU
  • WU XINCAN
  • HU LILING
  • FAN JIANDONG

Assignees

  • 浙江方格药业有限公司

Dates

Publication Date
20260505
Application Date
20260203

Claims (10)

  1. 1. The preparation method of the intestinal canal targeting type high-absorption ganoderma lucidum composite fermented beverage is characterized by comprising the following steps of: S1, raw material treatment, namely taking liquid submerged fermentation liquid of ganoderma lucidum mycelia, and carrying out solid-liquid separation to obtain supernatant A rich in triterpene compounds and mycelia for later use; S2, performing enzymatic compounding treatment, namely reacting the ganoderan with prebiotic oligosaccharide under the catalysis of transglycosidase, purifying reaction liquid to obtain a polysaccharide-prebiotic compound, mixing the compound with the supernatant A, and loading the triterpene compound to obtain a loaded compound system B; S3, in-situ nano assembly, namely mixing part of the supernatant A with tea polyphenol at 20-40 ℃ for 0.5-2 hours to form a composite core, sequentially adding a chitosan solution and a citrus pectin solution into the mixture under the condition of stirring or shearing, and forming a pH responsive coating outside the composite core through electrostatic layer-by-layer self-assembly to obtain nanoparticles A; s4, preparing a product, namely mixing the load composite system B, the nanoparticle A, the prebiotics and the auxiliary materials in an aqueous medium, sterilizing and filling to prepare the ganoderma lucidum composite fermentation drink.
  2. 2. The preparation method of the intestinal canal targeted high-absorption ganoderma lucidum composite fermented beverage according to claim 1, wherein in the step S2, the prebiotic oligosaccharide is selected from one or more of galacto-oligosaccharide, fructo-oligosaccharide and isomalto-oligosaccharide, the transglycosidase is selected from one of beta-galactosidase, fructo-transferase and beta-glucosidase, the adding amount of the transglycosidase is 6-20 enzyme activity units (U) per gram of ganoderma lucidum polysaccharide, the feeding mass ratio of the ganoderma lucidum polysaccharide to the prebiotic oligosaccharide is 1 (0.5-2), the reaction temperature is 35-55 ℃, the reaction pH is 5.5-7.5, and the reaction time is 4-10 hours.
  3. 3. The method for preparing an intestinal tract targeting type high-absorption ganoderma lucidum composite fermented beverage according to claim 1, wherein in the step S2, the purification treatment comprises the steps of inactivating enzyme of the reaction solution, performing dialysis or ultrafiltration to remove small molecular substances, and then performing drying.
  4. 4. The preparation method of the intestinal tract targeting type high-absorption ganoderma lucidum composite fermented beverage according to claim 1, wherein in the step S3, the tea polyphenol is selected from one of epigallocatechin gallate and theaflavin, the deacetylation degree of chitosan is more than or equal to 80%, and the esterification degree of citrus pectin is more than or equal to 60%.
  5. 5. The preparation method of the intestinal canal targeted high-absorption ganoderma lucidum composite fermented drink according to claim 1, wherein in the step S3, the feeding mass ratio of the triterpene compound, the tea polyphenol, the chitosan and the citrus pectin is 1 (0.2-2.5): 0.5-4): 1-7.
  6. 6. The method for preparing the intestinal tract targeting type high-absorption ganoderma lucidum composite fermented beverage according to claim 1, wherein in the step S3, the average particle size of the nano particles A is 50-300nm, the nano particles A are stable in structure in an environment with the pH less than or equal to 4.0, and the coating is dissociated in an environment with the pH more than or equal to 6.5.
  7. 7. The method for preparing the intestinal tract targeting type high-absorption ganoderma lucidum composite fermented beverage according to claim 1, wherein in the step S3, the process of adding the chitosan solution and the citrus pectin solution is carried out under the shearing condition with the rotating speed of 3000-15000 rpm.
  8. 8. An intestinal tract targeting type high-absorption ganoderma lucidum composite fermented beverage, which is characterized by being prepared by the method of any one of claims 1-7.
  9. 9. The intestinal tract-targeted high-absorption ganoderma lucidum composite fermented beverage according to claim 8, wherein the fermented beverage is a uniform and stable liquid system comprising nanoparticles having an average particle diameter of 50-300 nm and a pH-responsive coating, and a composite functional component comprising ganoderan, prebiotic oligosaccharide and ganoderma lucidum triterpene.
  10. 10. Use of the intestinal tract-targeted high-absorption ganoderma lucidum composite fermented beverage according to any one of claims 8 to 9 in the preparation of a food or health food for regulating intestinal flora and/or enhancing immunity of the body.

Description

Preparation method of intestinal targeting type high-absorption ganoderma lucidum composite fermented beverage Technical Field The invention relates to the technical field of functional food processing, in particular to a preparation method of an intestinal targeting type high-absorption ganoderma lucidum compound fermented beverage. Background The ganoderma lucidum fermentation product (comprising mycelium and fermentation liquor) is rich in bioactive substances such as polysaccharide, triterpene compounds and the like, has wide health benefits, however, the active ingredients, particularly hydrophobic triterpene substances, are easy to damage by gastric acid after being orally taken, have low absorption efficiency and unstable bioavailability in intestinal tracts, and in order to improve the situation, the prior art mainly adopts physical embedding (such as microcapsules) or simply mixes the active ingredients with prebiotics, the former is mostly suitable for solid dosage forms, is easy to leak and subside in liquid drinks, and the latter is easy to separate due to weak binding force among the ingredients, and cannot realize synchronous delivery and synergistic effect in the digestion process. Therefore, the ganoderma lucidum product preparation technology which can be used for a liquid system and realizing accurate intestinal targeting and efficient absorption of active ingredients is developed, and has important industrial value. Disclosure of Invention The invention aims to overcome the defects of the prior art and provide a preparation method of an intestinal targeting type high-absorption ganoderma lucidum compound fermented beverage with innovative process and remarkable effect and a product prepared by the method. In order to achieve the above purpose, the present invention provides the following technical solutions: A preparation method of an intestinal targeting type high-absorption ganoderma lucidum composite fermented beverage comprises the following steps: s1, raw material treatment, namely taking liquid submerged fermentation liquid of ganoderma lucidum mycelia, carrying out solid-liquid separation to obtain supernatant A rich in triterpene compounds and mycelia, and extracting and purifying the mycelia to obtain ganoderma lucidum polysaccharide. S2, performing enzymatic compound treatment, namely performing glycosylation reaction on the ganoderan and the prebiotic oligosaccharide under the catalysis of transglycosidase, wherein the addition amount of the transglycosidase is 6-20 enzyme activity units (U) per gram of ganoderan, purifying after the reaction is finished (for example, performing dialysis or ultrafiltration to remove small molecular substances after enzyme deactivation of a reaction solution, and then drying) to obtain a polysaccharide-prebiotic compound, and mixing the compound with the supernatant A to load triterpenes to obtain a load compound system B. S3, in-situ nano-assembly, namely mixing part of the supernatant A with tea polyphenol, stirring for 0.5-2 hours at 20-40 ℃, forming a structure taking a triterpene-tea polyphenol compound as a core through intermolecular action, sequentially adding a chitosan solution and a citrus pectin solution into the supernatant A under the action of stirring or shearing (3000-9000 rpm), and forming a pH responsive coating outside the compound core through electrostatic layer-by-layer self-assembly to obtain the nanoparticle A. S4, preparing a product, namely mixing the load compound system B, the nanoparticle A, the prebiotics and the auxiliary materials in an aqueous medium (conventional stirring can be adopted, such as 100-500rpm, or low-speed homogenization can be adopted), then carrying out pasteurization (72-85 ℃ for 15-30 seconds) or ultra-high temperature instantaneous sterilization (135-140 ℃ for 4-8 seconds), and finally carrying out aseptic filling to obtain the ganoderma lucidum compound fermented beverage. The enzymatic composite treatment step is not simple physical mixing of ganoderan and prebiotics, but rather a complex with a tighter and stable structure is formed by transglycosidase catalysis, and the loading capacity of the complex on triterpene components and the targeting of probiotics in intestinal tracts are obviously superior to those of the physical mixture. The auxiliary materials are pharmaceutically acceptable auxiliary materials, including but not limited to sweetening agents (such as sucralose and mogroside), acidulants (such as citric acid), stabilizing agents (such as xanthan gum), preservatives (such as potassium sorbate) and the like, and the dosage of the auxiliary materials is in accordance with the regulations of the national food safety standard food additive use standard (GB 2760-2014). Further, a liquid submerged fermentation broth of Ganoderma mycelia can be prepared by known techniques in the art, and the method comprises inoculating Ganoderma (Ganoderma lucidum) strain into a liquid fermentation