CN-121970894-A - Phospholipid complex with high bioavailability, and preparation method and application thereof

CN121970894ACN 121970894 ACN121970894 ACN 121970894ACN-121970894-A

Abstract

The invention discloses a phospholipid complex with high bioavailability and a preparation method and application thereof. The compound consists of 30-70 parts of nutrients, 15-50 parts of phospholipids, 2-10 parts of membrane layer framework materials, 0.2-2 parts of antioxidants, 5-30 parts of wall materials and 2-5 parts of adhesives, and has a phospholipid-wall material double-layer or multi-layer embedding structure. The preparation method comprises spray drying to prepare membranous phospholipid powder, modifying wall material with plasma, co-pulverizing, wet granulating, and cutting and spraying coating. The product has good fluidity, low solvent residue of <100 ppm, nutrient loss rate of <2%, 20-day retention rate acceleration of >90% at 60 ℃, bioavailability of 2-3 times of raw materials, and large-scale process and low cost. Is suitable for food, health product or medicine, and is used for efficiently delivering vitamins, minerals and other nutrients.

Inventors

WEI ZHIBO
REN XIANG
HONG YONGDE
CHEN JIANBIN
WU WENZHONG

Assignees

大连医诺生物股份有限公司

Dates

Publication Date: 20260505
Application Date: 20251231

Claims (13)

1. The phospholipid compound with high bioavailability is characterized by comprising, by weight, 30-70 parts of nutrients, 15-50 parts of phospholipids, 2-10 parts of membrane layer framework materials, 0.2-2 parts of antioxidants, 5-30 parts of wall materials and 2-5 parts of adhesives; The phospholipid is at least one of natural phospholipid, hydrogenated phospholipid and synthetic phospholipid; The membrane layer framework material is at least one selected from cholesterol, phytosterol, fatty acid ester of mono-diglycerol, fatty acid and vegetable oil; the antioxidant comprises a fat-soluble antioxidant and a water-soluble antioxidant; The fat-soluble antioxidant is at least one selected from ascorbyl palmitate, mixed tocopherol, alpha-tocopherol, tocotrienol, beta-carotene, lycopene, lutein, zeaxanthin, rosemary extract, carnosic acid, rosmarinic acid, quercetin, licoflavone, butyl hydroxy anisole, dibutyl hydroxy toluene, propyl gallate and tert-butyl hydroquinone. The water-soluble antioxidant is selected from at least one of ascorbic acid, sodium ascorbate, calcium ascorbate, D-isoascorbic acid, D-sodium erythorbate, disodium edetate, chlorogenic acid, cysteine, glutathione, tea polyphenol, citrus flavonoid and procyanidins; The wall material comprises at least one of sodium starch octenyl succinate, acacia octenyl succinate, corn starch, acacia, gelatin, carrageenan, pectin, xanthan gum, maltodextrin, solid corn syrup, resistant dextrin, inulin, isomaltooligosaccharide, maltitol, concentrated whey protein, isolated whey protein, hydrolyzed whey protein, zein, casein and sodium caseinate; The adhesive comprises at least one of methylcellulose, hydroxypropyl methylcellulose, sodium carboxymethylcellulose, hydroxypropyl cellulose, polyethylene glycol, acacia, pectin, gelatin and sodium alginate.
2. The phospholipid complex according to claim 1, wherein the phospholipid complex has a phospholipid-wall material bilayer or multilayer embedding structure composed of the membrane phospholipid and the wall material.
3. The phospholipid complex according to claim 1, wherein the nutrients are minerals, vitamins or a combination thereof.
4. The phospholipid complex according to claim 3, wherein the vitamin is at least one selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, and vitamin K.
5. The phospholipid complex according to claim 3, wherein the mineral is at least one selected from the group consisting of calcium, magnesium, zinc, iron, potassium, manganese, copper, sodium, chromium, selenium, iodine, and phosphorus.
6. A phospholipid complex according to claim 3, wherein: the mineral is selected from calcium glycinate, calcium L-aspartate, calcium L-lactate, calcium carbonate, calcium citrate malate, calcium fructoborate, calcium malate, calcium hydrogen phosphate, calcium chloride, tricalcium phosphate, calcium glycerophosphate, calcium oxide, calcium sulfate, calcium gluconate, magnesium chloride, magnesium carbonate, magnesium oxide, magnesium glycinate, magnesium gluconate, magnesium hydrogen phosphate, magnesium taurate, magnesium sulfate, magnesium lactate, magnesium malate, magnesium fumarate, magnesium threonate, magnesium succinate (magnesium succinate), magnesium L-aspartate, ferrous glycinate, ferrous sulfate, ferric pyrophosphate, ferrous fumarate, sodium ethylenediamine tetraacetate, ferrous gluconate, ferric citrate, ferrous citrate, ferric ammonium citrate, ferric phosphate, zinc glycinate, zinc gluconate, magnesium taurate, magnesium succinate, magnesium citrate, magnesium pyrophosphate, ferrous fumarate, ferrous pyrophosphate, ferrous fumarate, sodium ethylenediamine tetraacetate, ferrous gluconate, ferrous citrate, ferric citrate, zinc gluconate, sodium gluconate, and magnesium gluconate zinc sulfate, zinc gluconate, zinc citrate, zinc oxide, zinc lactate, zinc chloride, zinc acetate, zinc malate, zinc L-aspartate, manganese glycinate, manganese sulfate, manganese chloride, manganese carbonate, manganese gluconate, manganese citrate, manganese L-aspartate, copper sulfate, copper glycinate, copper gluconate, copper citrate, copper carbonate, potassium chloride, potassium citrate, potassium dihydrogen citrate, potassium L-aspartate, potassium gluconate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium hydrogen carbonate, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium citrate, sodium chloride, sodium L-aspartate, sodium selenite, sodium selenate, potassium iodide, potassium iodate, sodium iodide, chromium sulfate, chromium chloride.
7. The phospholipid complex according to claim 1, wherein the weight ratio of the membrane layer framework material to the phosphatidylcholine component in the phospholipid is 1:3-1:15, and the phosphatidylcholine content in the phospholipid is more than 50wt%.
8. The method for preparing a phospholipid complex according to claim 1, comprising the steps of: s1, dissolving phospholipid, a membrane layer framework material, fat-soluble nutrients and fat-soluble antioxidants in an organic solvent at 40-80 ℃ and performing spray drying to obtain membranous phospholipid powder; S2, carrying out low-temperature plasma treatment on the wall material to obtain a modified wall material; s3, fully mixing and crushing the membranous phospholipid powder prepared in the step S1 and non-fat-soluble nutrients; S4, completely dissolving the water-soluble antioxidant and the adhesive in water to obtain granulating slurry, performing wet granulation on the material prepared in the step S3, adding part of the modified wall material obtained in the step S2 after preliminary molding, and continuously granulating, molding and finishing to obtain a phospholipid compound with uniform granularity; S5, dispersing the modified wall materials remained after the step S4 is used in water to prepare coating liquid, and coating and drying the phospholipid complex obtained in the step S4 serving as a base material to obtain a final product.
9. The method of claim 8, wherein the spray drying in S1 is performed by a closed cycle spray drying process, and the inlet air temperature is controlled to be 70-110 ℃.
10. The method of claim 8, wherein the pulverizing means is jet milling, and the particle size D90 after pulverizing is 10 μm to 100. Mu.m.
11. The method of claim 8, wherein the S4 wet granulation is performed by adding 60 to 95wt% of the modified wall material prepared by the S2 to the total amount during granulation.
12. The method of claim 8, wherein the coating in S5 is a cutting and spraying coating, and the air inlet temperature is controlled to be 40-70 ℃ and the material temperature is maintained to be 35-60 ℃ in the coating process.
13. Use of a phospholipid complex as defined in claim 1 in the manufacture of a food, health product or medicament for improving the bioavailability and stability of a nutrient.

Description

Phospholipid complex with high bioavailability, and preparation method and application thereof Technical Field The invention belongs to the field of food nutrition and health, and particularly relates to a phospholipid complex with high bioavailability, a preparation method and application thereof. Background Minerals and vitamins are micronutrients necessary to maintain normal physiological functions in humans. However, these nutrients present challenges in practical applications, and many mineral ions are susceptible to pH, phytic acid, oxalic acid, etc. in the gastrointestinal tract, forming insoluble precipitates, resulting in low absorption rates. Vitamins, especially water-soluble vitamins, are easy to lose during production, processing and storage, and fat-soluble vitamins have the problems of difficult dissolution and low bioavailability. Furthermore, interactions exist between some vitamins and minerals, accelerating degradation. To improve the stability and bioavailability of these nutrients, microencapsulation techniques are widely used. The prior art includes spray-dried embedding, phospholipid encapsulation, and the like. However, although microencapsulation techniques are widely explored, existing methods have inherent contradictions in technical paths, often in mind. The single spray drying technology has limited embedding efficiency on minerals, and the traditional phospholipid technology can improve bioavailability, but has the defects of easy leakage in the storage process, high mass production cost and the like. Chinese patent CN115006410B discloses a compound vitamin liposome and a preparation method and application thereof, wherein components such as vitamin and the like are required to be heated and melted at 150-160 ℃ in the preparation process, and a large amount of degradation can be caused to thermosensitive vitamin components, so that decomposition products harmful to human bodies can be possibly generated, and the quality of the products is difficult to ensure. Patent CN109497561B discloses a preparation method of vitamin B12 nanoliposome, which adopts means such as film hydration method, ultrasonic cell pulverization, low-temperature centrifugation, microporous filter membrane filtration, etc., although the technology can realize higher encapsulation efficiency, the difficulty of the process for amplifying production is high, and the temperature requirement of a rotary evaporator is less than or equal to 20 ℃ when the solvent is removed, so that the residual level of the solvent in the product is difficult to be ensured. Patent CN120549866A discloses a calcium liposome for promoting bone health, a preparation method and application thereof, and an embodiment in the patent shows a higher bioavailability effect, but a phospholipid composition in a formula consists of egg yolk lecithin, phosphatidylinositol and distearoyl phosphatidylcholine, the mass ratio of the egg yolk lecithin to the phosphatidylinositol to the distearoyl phosphatidylcholine is 8-12:7-11:1-5, and in fact, the phospholipid is a mixture, and only the proportion of the amount is regulated, so that the purity of the phospholipid raw material is not limited, and the quality of a product among batches is possibly unstable. Therefore, there is an urgent need in the art for an innovative method and product that can fundamentally break through the limitations of the above technical paths, is stable, continuous and suitable for mass production, and can significantly improve the stability and bioavailability thereof. Disclosure of Invention Aiming at the problems of poor stability, limited absorption effect, difficult mass production and the like of mineral and vitamin components in the background art, the invention provides a phospholipid complex with high bioavailability and a preparation method thereof. The phospholipid composite with high bioavailability comprises, by weight, 30-70 parts of nutrients, 15-50 parts of phospholipids, 2-10 parts of membrane layer framework materials, 0.2-2 parts of antioxidants, 5-30 parts of wall materials and 2-5 parts of binders. The more preferable weight parts are 30-48 parts of nutrients, 28-50 parts of phospholipids, 3-8.5 parts of film skeleton materials, 1-2 parts of antioxidants, 10-30 parts of wall materials and 2.5-4 parts of adhesives, wherein: The phospholipid is at least one of natural phospholipid, hydrogenated phospholipid and synthetic phospholipid; the film framework material is at least one selected from cholesterol, phytosterol and phytosterol ester; the antioxidant comprises a fat-soluble antioxidant and a water-soluble antioxidant; The fat-soluble antioxidant is at least one selected from ascorbyl palmitate, mixed tocopherol, alpha-tocopherol, tocotrienol, beta-carotene, lycopene, lutein, zeaxanthin, rosemary extract, carnosic acid, rosmarinic acid, quercetin, licoflavone, butyl hydroxy anisole, dibutyl hydroxy toluene, propyl gallate and tertiary butyl hydroquinon