CN-121970898-A - Polysaccharide substance encapsulated resveratrol compound and preparation method and application thereof

Abstract

The invention relates to a resveratrol complex encapsulated by polysaccharide substances, a preparation method and application thereof, wherein the resveratrol complex comprises resveratrol and polysaccharide substances encapsulated by resveratrol, the polysaccharide substances are linear polysaccharide, the resveratrol can induce polysaccharide chains to be unfolded so that the resveratrol can be primarily combined through hydrogen bonding, and then the resveratrol is reacted under the condition of pH 6.0-9.0, so that the dual effects of self-enhancement of a carrier (polysaccharide substances) and stabilization of a guest (resveratrol) are realized, the antioxidant activity of the polysaccharide is enhanced through nanocrystallization transformation, and the retention rate and stability of the resveratrol are improved through encapsulation.

Inventors

• YAN JINXIN
• QI XIANGYANG
• RUAN DI
• SHA JIN
• CAO SHAOQIAN
• YANG HUA

Assignees

• 浙江万里学院

Dates

Publication Date

20260505

Application Date

20260203

Claims (6)

1. 1. A resveratrol compound encapsulated by polysaccharide is characterized by comprising resveratrol and polysaccharide substances encapsulated by resveratrol, wherein the polysaccharide substances are linear polysaccharide, and the resveratrol can induce polysaccharide chains to be unfolded so that the resveratrol and the polysaccharide chains are initially combined through hydrogen bonding and then react under the condition of pH 6.0-9.0.
2. 2. The polysaccharide encapsulated resveratrol complex according to claim 1, wherein the linear polysaccharide is extracted from Dendrobium officinale.
3. 3. The polysaccharide encapsulated resveratrol complex according to claim 2, wherein the linear polysaccharide is at least one of stem polysaccharide extracted from Dendrobium officinale stem, leaf polysaccharide extracted from Dendrobium officinale leaf, and flower polysaccharide extracted from Dendrobium officinale flower.
4. 4. The polysaccharide-encapsulated resveratrol complex according to claim 3, wherein the extraction method of the linear polysaccharide is as follows: (1) Raw material pretreatment, namely respectively drying stems, leaves and flowers of dendrobium candidum to constant weight, crushing and sieving to obtain stem powder, leaf powder and flower powder, wherein the stem powder, the leaf powder and the pollen powder are collectively called as powder, and the treatment method of each powder is as follows; (2) Pigment removal, namely adding an ethanol solution into the powder, soaking in a dark place until the color of the solution is changed from light pink to colorless, and collecting solid precipitate after solid-liquid separation; (3) Adding deionized water and compound enzyme into the solid sediment in the step (2), carrying out constant-temperature water bath at 55-60 ℃ for 65-70 min, and then carrying out solid-liquid separation to collect supernatant, wherein the mass ratio of the solid sediment to the deionized water is 1:10-1:20, the addition ratio of the compound enzyme to the solid sediment is 300-1500U/g, the compound enzyme consists of pectase, cellulase and papain, the addition ratio of pectase to the solid sediment is 100-500U/g, the addition ratio of cellulase to the solid sediment is 100-500U/g, and the addition ratio of papain to the solid sediment is 100-500U/g; (4) And (3) alcohol precipitation and aging, namely adding absolute ethyl alcohol into the supernatant fluid obtained in the step (3), standing at 2-4 ℃ until no solid precipitate is generated in the absolute ethyl alcohol, carrying out solid-liquid separation to obtain a precipitate, and freeze-drying the precipitate to obtain powdery polysaccharide substances.
5. 5. A method for preparing the polysaccharide substance encapsulated resveratrol complex according to any of claims 1-4, which is characterized by comprising the following steps: dissolving polysaccharide substances in deionized water to prepare polysaccharide stock solution with the concentration of 1-5 mg/mL, dissolving resveratrol in ethanol solution to prepare resveratrol stock solution with the concentration of 1.00-1.50 mg/mL; Dropwise adding the resveratrol stock solution into the polysaccharide stock solution according to the volume ratio of 1:10-1:12 under the stirring condition, wherein in the process, resveratrol induces the polysaccharide chain to be unfolded, so that the resveratrol and the polysaccharide chain are initially combined through hydrogen bonding, and then the pH of a system is regulated to be 6.0-9.0,35-38 ℃ and stirred for 1-1.5-h to form a mixed solution; And thirdly, carrying out solid-liquid separation on the mixed solution, wherein the obtained clarified liquid is the resveratrol complex solution encapsulated by polysaccharide substances, and further carrying out freeze drying to obtain the solid nano particles.
6. 6. The application of the polysaccharide substance encapsulated resveratrol compound according to any of claims 1-4, which is characterized in that the polysaccharide substance encapsulated resveratrol compound can be eaten as an antioxidant functional food.

Description

Polysaccharide substance encapsulated resveratrol compound and preparation method and application thereof Technical Field The invention relates to the technical field of encapsulation compounds, in particular to a resveratrol complex encapsulated by polysaccharide substances, a preparation method and application thereof. Background Hydrophobic bioactive substances (such as resveratrol) have poor water solubility and weak environmental stability (easy to be degraded by heat, light and ions), and limit the application of the hydrophobic bioactive substances in foods. In view of the above problems, the Chinese patent application No. CN201910556255.9 (publication No. CN 112120945A) discloses a water-in-water composition containing resveratrol in high content, which comprises an internal phase containing a polyol polymer with a molecular weight ranging from 200 to 600 g/mol and resveratrol, wherein the weight ratio of the polyol polymer to resveratrol is equal to or greater than 10:1, the content of the polyol polymer is less than 55% by weight and the content of resveratrol is up to 5% by weight based on the total weight of the composition, and an external phase containing a natural high molecular polysaccharide polymer with a molecular weight ranging from 2X 105 to 5X 105 g/mol, the content of the natural high molecular polysaccharide polymer being from 1% by weight to 10% by weight based on the total weight of the composition. The patent improves the stability and water solubility of resveratrol by means of a polyol polymer and a high molecular polysaccharide polymer. The external phase (natural high molecular polysaccharide polymer) of this patent is used only as a "protection/delivery tool" to improve the solubility, stability or bioavailability of resveratrol, while the function of the external phase itself is not enhanced. Disclosure of Invention The first technical problem to be solved by the invention is to provide a polysaccharide substance for encapsulating resveratrol compound, wherein the polysaccharide substance can improve the performance of resveratrol, and the polysaccharide substance can also improve the performance in the process. The second technical problem to be solved by the invention is to provide a preparation method of the resveratrol complex encapsulated by polysaccharide substances aiming at the current state of the art. The second technical problem to be solved by the invention is to provide an application of the polysaccharide substance encapsulated resveratrol compound aiming at the current state of the art. The technical scheme adopted by the invention for solving the first technical problem is that the resveratrol compound encapsulated by polysaccharide substances is characterized by comprising resveratrol and polysaccharide substances encapsulating resveratrol, wherein the polysaccharide substances are linear polysaccharides, and the resveratrol can induce polysaccharide chains to be unfolded so that the resveratrol and the resveratrol are initially combined through hydrogen bonding, and then react under the condition of pH 6.0-9.0. Preferably, the linear polysaccharide is extracted from Dendrobium officinale. Dendrobium officinale is used as a medicine and food homologous raw material, and polysaccharide (DOP) of the dendrobium officinale has excellent biological activities such as antioxidation, immunoregulation and the like, and is strong in thermal stability and good in film forming property, and is a potential natural bioactive substance embedding carrier. The complex of the invention has good biocompatibility, polysaccharide substance is derived from natural medicinal and edible raw materials, has no toxic or side effect and high biological safety, and is suitable for the field of functional foods. Preferably, the linear polysaccharide is at least one of stem polysaccharide extracted from dendrobium candidum stems, leaf polysaccharide extracted from dendrobium candidum leaves and flower polysaccharide extracted from dendrobium candidum flowers. The dendrobium candidum polysaccharide has high utilization rate, reduces resource waste, brings dendrobium candidum leaves and flowers byproducts into polysaccharide extraction raw materials, has good free radical scavenging capability for stem polysaccharide (DOSP), leaf polysaccharide (DOLP) and flower polysaccharide (DOFP), has the potential of antioxidant functional food development, and can realize comprehensive utilization of whole plant resources. Wherein, the flower polysaccharide (DOFP) brings more hydrogen bond sites due to high galacturonic acid content and branched structure, and forms more hydrogen bonds with resveratrol, so that the embedding stability is optimal. In the above scheme, the extraction method of the linear polysaccharide comprises the following steps: (1) Raw material pretreatment, namely respectively drying stems, leaves and flowers of dendrobium candidum to constant weight, crushing and sieving to obta