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CN-121970902-A - Preparation method of fungus polysaccharide adjuvant drug composite component and fungus component composite

CN121970902ACN 121970902 ACN121970902 ACN 121970902ACN-121970902-A

Abstract

The invention is suitable for the technical field of functional food preparation, and provides a preparation method of a fungus polysaccharide adjuvant drug composite component and a fungus component composite, wherein the preparation method comprises the steps of firstly carrying out physical adsorption and function integration extraction on four adjuvant materials such as mushrooms, pleurotus nebrodensis and the like by adopting granular and dissolved pachymaran in sequence through a step-by-step guiding extraction process, then applying an ultrasonic wave field to carry out dynamic co-coagulation in a low-temperature vacuum concentration process, and finally forming to obtain 100-500 mu m adjuvant drug composite gel particles; the invention realizes the controllable materialization of the Chinese medicine monarch and minister medicine compatibility theory through the multiple physical structure design, and the prepared product has the advantages of ingredient synergy, programmed release and remarkable improvement of immunoregulation activity.

Inventors

  • LIU SHIZHU
  • WU XINCAN
  • HU LILING
  • FAN JIANDONG

Assignees

  • 浙江方格药业有限公司

Dates

Publication Date
20260505
Application Date
20260203

Claims (10)

  1. 1. The preparation method of the fungus polysaccharide adjuvant drug composite component is characterized by comprising the following steps: s1, step-by-step guiding extraction: S1a, physical adsorption guiding, namely mixing dry powder of four raw materials of lentinus edodes, pleurotus nebrodensis, white back fungus and agaricus bisporus, forming an extraction system together with water and granular pachyman, and carrying out first-stage extraction at 40-60 ℃; s1b, function integration guiding, namely adding a dissolved pachymaran solution into the system of S1a, adjusting the pH of the system, performing second-stage extraction at 60-95 ℃, and separating after the extraction is finished to obtain a first composite polysaccharide extract; S2, dynamic co-coagulation concentration, namely carrying out low-temperature vacuum concentration on the first composite polysaccharide extract, and applying ultrasonic waves to the first composite polysaccharide extract in the concentration process to obtain composite polysaccharide colloid; s3, forming the composite component, namely preparing the composite polysaccharide colloid into gel particles with the size of 100-500 microns to obtain the adjuvant drug composite component.
  2. 2. The method for preparing the fungus polysaccharide adjuvant drug composite component according to claim 1, wherein in the step S1a, the mass ratio of the dried powder of the mushrooms, the pleurotus nebrodensis, the white back fungus and the agaricus bisporus is 1-3:1-2:1-2:1-3, the particle size of the granular pachyman is 1-100 micrometers, the addition amount of the granular pachyman is 10-15% of the total mass of the mixed powder of four raw materials, the first extraction time is 0.5-2 hours, the feed liquid ratio is 1:10-20 (g/mL), the concentration of the dissolved pachyman is 1-3% (w/v), the addition amount of the granular pachyman is 5-10% of the total mass of the mixed powder of the four raw materials based on dry matter, the second extraction time is 1-4 hours, and the pH is 4.0-6.0.
  3. 3. The method for preparing the polysaccharide adjuvant drug composite according to claim 1, wherein in the step S2, the condition of low-temperature vacuum concentration is that the initial temperature is 60 ℃, the vacuum degree is maintained at-0.06 to-0.1 MPa, a program gradient cooling mode is adopted, the temperature is uniformly cooled to 40 ℃ at the speed of 0.5 to 1 ℃ per minute until the material is concentrated to 10 to 20 percent of the original volume, the applied ultrasonic frequency is 20 to 40kHz, and the power density is 50 to 200W/L.
  4. 4. The method for preparing a complex of a polysaccharide adjuvant and a drug according to claim 1, wherein in step S3, the shaping method comprises one of spray granulation, drop forming or extrusion rounding.
  5. 5. A fungus polysaccharide adjuvant drug composite component, characterized in that it is prepared by the method of any one of claims 1-4.
  6. 6. The fungus composition complex is characterized by comprising the fungus polysaccharide adjuvant drug composite component, a monarch drug and a ministerial drug according to claim 5, wherein the monarch drug comprises grifola frondosa polysaccharide and Cordyceps militaris polysaccharide, and the ministerial drug comprises ganoderma sinensis extract, hericium erinaceus polysaccharide and agaricus blazei polysaccharide.
  7. 7. The fungus composition according to claim 6, wherein the fungus polysaccharide adjuvant comprises, by weight, 24-36 parts of a fungus polysaccharide, 12-17 parts of a grifola frondosa polysaccharide, 12-17 parts of a Cordyceps militaris polysaccharide, 15-20 parts of a Ganoderma sinensis extract, 10-14 parts of a Hericium erinaceus polysaccharide, and 8-12 parts of an agaricus blazei polysaccharide.
  8. 8. The method for preparing the microbial biomass ingredient complex according to claim 6, comprising the steps of: P1 preparing solid core of monarch drug, comprising mixing Maitake Mushroom polysaccharide, cordyceps militaris polysaccharide, ganoderma extract, hericium erinaceus polysaccharide and Agaricus blazei polysaccharide, and granulating by dry method to obtain solid pellet with particle diameter of 100-1000 μm; P2. constructing a core-shell structure, namely placing a monarch drug solid phase core in fluidized bed coating equipment to keep a fluidized state, dispersing or dissolving a fungus polysaccharide adjuvant drug composite component in an adhesive solution to be used as coating slurry, uniformly coating the coating slurry on the surface of the fluidized monarch drug solid phase core through a fluidized bed spraying process to form a coating layer, and drying to obtain a micro unit, wherein the dry weight of the coating layer accounts for 20% -40% of the total weight of the micro unit; And P3, final shaping, namely drying the microcells to ensure that the water content of the microcells is lower than 5 percent, and obtaining the bacterial composition compound.
  9. 9. The method of claim 8, wherein in step P2, the fluidized bed spraying process is a bottom spraying process or a tangential spraying process, and the binder solution is an aqueous solution of hypromellose or an aqueous solution of sodium alginate having a concentration of 1% -5% (w/v).
  10. 10. The use of the fungus composition of claim 6 for preparing health food or medicine for regulating immunity, warming yang, strengthening body resistance or improving deficiency-cold syndrome.

Description

Preparation method of fungus polysaccharide adjuvant drug composite component and fungus component composite Technical Field The invention belongs to the technical field of functional food preparation, and particularly relates to a preparation method of a fungus polysaccharide adjuvant drug composite component and a fungus component composite. Background In the traditional Chinese medicine compound preparation, the compatibility theory of 'monarch, minister, assistant and guide' is essence, wherein 'adjuvant and drug' is commonly used for assisting monarch, minister and drug, or is used for eliminating and weakening toxicity or intensity of monarch and drug or treating concurrent diseases, and 'messenger and drug' plays roles of harmonizing various drugs and guiding drug efficacy to reach the place of disease, however, in modern industrial production, how to convert the abstract theory into a specific, controllable and repeatable preparation technology is a great difficulty. In the prior art, although an attempt is made to compound a plurality of mushroom polysaccharides, the process nature is still simple superposition of components, and structural integration based on function guidance cannot be realized, for example, a method for preparing a compound polysaccharide nutrient solution is disclosed in a comparison document CN101228951B, wherein a plurality of strains such as ganoderma lucidum, poria cocos, mushroom, pleurotus nebrodensis and the like are respectively fermented and respectively extracted, and then the culture solutions are physically mixed. This method has the following inherent drawbacks: in the method, tuckahoe is equivalent to other strains in position, is added as one of common active ingredients, and is not designed and embodied at all in the process as the core function of harmonizing various medicines and guiding medicines to channel. The lack of structural guidance refers to the lack of targeted utilization of pachyman physical forms (particle and dissolved) in the process, and the pachyman can not be used as a structural core or a guiding agent to actively adsorb and integrate other polysaccharide molecules. The components are still physically discrete after mixing, as if a disc were sanded. The product structure is loose, the final product is simply a simple blend of a plurality of polysaccharide molecules, and the components lack stable interaction and ordered spatial arrangement, so that physical and chemical properties (such as stability and release behavior) and biological functions (such as cooperativity and targeting) of the product cannot be improved. Therefore, when the simple mixed adjuvant and guiding medicine components are compatible with monarch medicines and ministerial medicines, the dredging effect is disordered and free, the orderly and synergistic effect cannot be realized, even the stability of the whole prescription is possibly influenced due to mutual interference of the devitrification, and how to materialize the traditional Chinese medicine adjuvant and guiding pharmacology theory, particularly the regulating and guiding functions of the medicines through a quantifiable and controllable modern preparation process becomes a technical problem which is not solved in the field for a long time. Disclosure of Invention The invention provides a preparation method of a fungus polysaccharide adjuvant drug composite component and a fungus component composite, and aims to solve the problems. The invention discloses a preparation method of a fungus polysaccharide adjuvant drug composite component, which comprises the following steps: s1, step-by-step guiding extraction: S1a, physical adsorption guiding, namely mixing dry powder of four raw materials of lentinus edodes, pleurotus nebrodensis, white back fungus and agaricus bisporus, forming an extraction system together with water and granular pachyman, and carrying out first-stage extraction at 40-60 ℃; s1b, function integration guiding, namely adding a dissolved pachymaran solution into the system of S1a, adjusting the pH of the system, performing second-stage extraction at 60-95 ℃, and separating after the extraction is finished to obtain a first composite polysaccharide extract; The method comprises the steps of taking tiny pachyman particles as seed crystals or anchor points to adsorb other polysaccharide molecules, and then taking dissolved pachyman as a functional medium to further guide and integrate the adsorbed polysaccharide active fragments in the solution. S2, dynamic co-coagulation concentration, namely carrying out low-temperature vacuum concentration on the first composite polysaccharide extract, and applying ultrasonic waves to the first composite polysaccharide extract in the concentration process to obtain composite polysaccharide colloid; At the same time of vacuum concentration, by applying low-intensity ultrasonic waves with specific power, irregular thermal movement is broken, so that polysaccharide