CN-121970904-A - Oyster peptide complex for promoting postoperative recovery, protecting liver and dispelling effects of alcohol and preparation method thereof

Abstract

The invention relates to an oyster peptide compound for promoting postoperative recovery, protecting liver and dispelling effects of alcohol and a preparation method thereof. The compound mainly comprises modified oyster peptide and dihydromyricetin, and is matched with an antioxidant, a cross-linking agent, an adhesive and a pH regulator, and the stability, bioavailability and release control capability of the oyster peptide are improved through a chemical modification method. The compound has obvious functions of protecting liver, dispelling alcohol effect, resisting oxidation and promoting postoperative recovery. The oyster peptide is modified through esterification reaction, and the oyster peptide and small organic molecules such as dihydromyricetin are synergistic, so that the in vivo effect of the oyster peptide is improved. The compound provided by the invention has excellent performances in promoting postoperative recovery, improving hemostatic effect, enhancing antioxidant capacity and accelerating biodegradation, has a wide application prospect, and is particularly suitable for the fields of functional foods and health care products.

Inventors

• LIU HAIXIA
• JIANG XINHUI
• WEN HAILAN
• JIANG MIN
• JIANG MINGFU
• LIU ZHONGMING
• JIANG QIUPING
• LAN CHENGMU
• ZHENG JIABIN

Assignees

• 福建大众健康生物科技有限公司
• 福州日兴水产食品有限公司

Dates

Publication Date

20260505

Application Date

20260206

Claims (9)

1. 1. The oyster peptide compound is characterized by comprising, by weight, 60-80 parts of modified oyster peptide, 10-20 parts of dihydromyricetin, 1-5 parts of antioxidant, 0.5-1 part of cross-linking agent, 1-3 parts of adhesive and 0.1-0.5 part of pH regulator, wherein the oyster peptide is subjected to chemical modification treatment, and the specific chemical modification method comprises the steps of reacting the oyster peptide with fatty acid, and reacting for 6-12 hours at the temperature of 60-80 ℃ by adopting esterification reaction to obtain esterified oyster peptide with higher stability, wherein the dihydromyricetin is an organic micromolecule which is not applied to the field.
2. 2. The oyster peptide complex for promoting postoperative recovery, protecting liver and dispelling effects of alcohol according to claim 1, wherein the modified oyster peptide comprises the following raw materials, by weight, 60-80 parts of oyster peptide, 5-10 parts of fatty acid, 0.5-1 part of glutaraldehyde and 10-15 parts of dichloromethane.
3. 3. The oyster peptide complex for promoting postoperative recovery and protecting liver from alcohol according to claim 1 or 2, wherein the preparation method of the modified oyster peptide comprises the following steps: (1) Dissolving oyster peptide in proper amount of deionized water to obtain homogeneous solution; (2) Adding fatty acid and glutaraldehyde into the uniform solution, and uniformly stirring; (3) Adding methylene dichloride into the solution to perform esterification reaction; (4) After the reaction is finished, unreacted raw materials are removed through filtration and washing, and modified oyster peptides are obtained; (5) And performing freeze drying treatment on the modified oyster peptide to obtain a final modified oyster peptide product.
4. 4. The oyster peptide complex for promoting postoperative recovery and protecting liver from alcohol according to claim 3, wherein the reaction condition in the step (3) is that the temperature is 60-80 ℃ and the reaction time is 6-12 hours.
5. 5. The oyster peptide complex for promoting postoperative recovery and protecting liver and alleviating hangover according to claim 3, wherein the drying treatment condition in the step (5) is that the temperature is 40-60 ℃, the drying time is 12-24 hours, and the treatment is performed by using vacuum drying equipment.
6. 6. The oyster peptide complex for promoting postoperative recovery and protecting liver from alcohol according to claim 1, wherein the antioxidant is vitamin E, the crosslinking agent is glutaraldehyde, the adhesive is glycerol, and the pH regulator is sodium citrate.
7. 7. A method for preparing an oyster peptide complex for promoting postoperative recovery and protecting liver from alcohol effect, which is characterized by comprising the following steps of: S1, mixing the modified oyster peptide with dihydromyricetin, vitamin E, glycerol and sodium citrate, and uniformly stirring to obtain a compound; S2, drying the compound to obtain the oyster peptide compound for promoting postoperative recovery, protecting liver and dispelling effects of alcohol.
8. 8. The oyster peptide complex for promoting postoperative recovery and protecting liver and alleviating hangover according to claim 1, wherein the mixing condition in the step S1 is that the stirring speed is 200-500 rpm, and the mixing time is 30-60 minutes.
9. 9. The oyster peptide complex for promoting postoperative recovery and protecting liver and alleviating hangover according to claim 1, wherein the drying treatment condition in the step S2 is that the temperature is 40-60 ℃, the drying time is 12-24 hours, and the processing is performed by using vacuum drying equipment.

Description

Oyster peptide complex for promoting postoperative recovery, protecting liver and dispelling effects of alcohol and preparation method thereof Technical Field The invention relates to preparation of bioactive substances and application thereof, in particular to oyster peptide composite for promoting postoperative recovery and protecting liver and dispelling effects of alcohol and a preparation method thereof. Background Oyster peptide is used as a natural bioactive substance, is rich in amino acids, microelements and polypeptide components, and has various physiological functions of protecting liver, relieving alcoholism, resisting oxidation, regulating immunity, promoting cell regeneration and the like. In recent years, oyster peptide is widely applied to health-care food, functional beverage and nutritional supplement, especially in the fields of liver protection, postoperative recovery, anti-alcohol and the like, and has a good application prospect. Although the bioactive components of oyster peptides have remarkable effects, there are still some problems in the prior art in the use of oyster peptides. Firstly, oyster peptide has poor stability in vivo and is easy to be degraded by digestive enzymes, so that active ingredients of the oyster peptide are lost, secondly, the oyster peptide has low bioavailability and limited absorption efficiency, the due therapeutic effect of the oyster peptide is difficult to be exerted, and finally, the problem of release control of the oyster peptide is not solved effectively, the release time and the release rate of the oyster peptide are unstable, and the fluctuation and the persistence of the curative effect are possibly poor. In order to address these problems, studies have been proposed to improve the stability and bioactivity of oyster peptides by chemical modification means. The oyster peptide is modified through esterification reaction and crosslinking reaction, so as to enhance the stability in vivo, improve the bioavailability and prolong the duration of action in vivo. However, oyster peptide compounds for specific application fields such as postoperative recovery, liver protection and anti-alcohol are not yet seen at present, and the optimization effect of oyster peptide compounds still has a certain limit. Therefore, there is a need to develop an innovative oyster peptide complex, which is combined with other functional components by modifying means to enhance its biological activity, and to improve its stability, release control ability and bioavailability, especially in the field of postoperative recovery and liver protection. Disclosure of Invention In order to overcome the defects of the oyster peptide in the aspects of stability, bioavailability and release control in the prior art, the invention aims to provide an oyster peptide compound for promoting postoperative recovery, protecting liver and dispelling effects of alcohol and a preparation method thereof. The compound is prepared by carrying out chemical modification on oyster peptide, adopting esterification reaction to improve the stability and the biological activity of the oyster peptide, and combining small organic molecules such as dihydromyricetin which are not applied to the field to form the compound with synergistic effect. The compound has obvious postoperative recovery, liver protection and alcohol effect relieving functions, improves the stability, bioavailability and controlled release capability of oyster peptides through an innovative modification method, and can provide lasting curative effect. The oyster peptide complex has excellent effects in promoting postoperative recovery, protecting liver and dispelling effects of alcohol, and is remarkably improved in bioavailability and stability. The aim of the invention can be achieved by the following technical scheme: The oyster peptide compound comprises, by weight, 60-80 parts of modified oyster peptide, 10-20 parts of dihydromyricetin, 1-5 parts of an antioxidant, 0.5-1 part of a cross-linking agent, 1-3 parts of an adhesive and 0.1-0.5 part of a pH regulator, wherein the oyster peptide is subjected to chemical modification treatment, and the specific chemical modification method comprises the steps of reacting the oyster peptide with fatty acid for 6-12 hours at the temperature of 60-80 ℃ by adopting an esterification reaction to obtain esterified oyster peptide with higher stability, and the dihydromyricetin is an organic small molecule which is not applied to the field, and has the function of promoting postoperative recovery and protecting liver from alcohol. Optionally, the modified oyster peptide comprises the following raw materials, by weight, 60-80 parts of oyster peptide, 5-10 parts of fatty acid, 0.5-1 part of glutaraldehyde and 10-15 parts of dichloromethane. Optionally, the preparation method of the modified oyster peptide comprises the following steps: (1) Dissolving oyster peptide in proper amount of deionized water t