CN-121971081-A - Imaging method, imaging system, storage medium and computing device

CN121971081ACN 121971081 ACN121971081 ACN 121971081ACN-121971081-A

Abstract

The application provides an imaging method, a system, a storage medium and a computing device, wherein the method comprises the steps of installing an oxygen sensitive film with an oxygen sensitive probe, which is prepared in advance, on a tissue to be detected of an object to be detected, wherein the tissue to be detected comprises specific tissue cells with calcium signal sensitivity, alternately radiating a plurality of types of exciting light on the tissue to be detected according to a target interval through an imaging passage, respectively and continuously acquiring first exciting light signals of frames on the oxygen sensitive film, second exciting light signals of frames of specific tissue cells and reflected light signals of frames of blood oxygen in the tissue to be detected by utilizing different cameras according to the target interval, generating a tissue oxygen partial pressure image of the tissue to be detected according to the continuously acquired first exciting light signals of frames, generating a calcium signal image of the tissue to be detected according to the continuously acquired second exciting light signals of frames, and generating a blood oxygen signal image of the tissue to be detected according to the continuously acquired reflected light signals of frames.

Inventors

SHENG XING
SHENG DAWEI
LIN QINGYI
WANG XUYI

Assignees

清华大学

Dates

Publication Date: 20260505
Application Date: 20260112

Claims (11)

1. A method of imaging, the method comprising: installing an oxygen sensitive film with an oxygen sensitive probe prepared in advance on a tissue to be detected of an object to be detected, wherein the tissue to be detected comprises specific tissue cells with calcium signal sensitivity; alternately irradiating a plurality of types of excitation light on the tissue to be detected according to a target interval through an imaging passage; according to the target interval, respectively and continuously acquiring each frame of first excitation light signals on the oxygen sensitive film, each frame of second excitation light signals of the specific tissue cells and each frame of reflected light signals of blood oxygen in the tissue to be detected by using different cameras; Generating a tissue oxygen partial pressure image of the tissue to be detected according to the first excitation light signals of each frame which is continuously collected, generating a calcium signal image of the tissue to be detected according to the second excitation light signals of each frame which is continuously collected, and generating a blood oxygen signal image of the tissue to be detected according to the reflected light signals of each frame which is continuously collected.
2. The method of claim 1, wherein the oxygen sensitive film is prepared by: Preparing dendritic mesoporous silica nano particles DMSN by using hexadecyl trimethyl ammonium p-toluenesulfonate, triethanolamine and deionized water; preparing dendritic mesoporous silica nanoparticles Ru (dpp) 3 Cl 2 @ DMSN loaded with Ru (dpp) 3 Cl 2 by using a silica suspension prepared based on the DMSN and a sodium hydroxide aqueous solution and a mixed solution of tris (4, 7-phenyl-1, 10-phenanthroline) ruthenium (II) di (ester) Ru (dpp) 3 Cl 2 and an absolute ethanol solution; The oxygen sensitive film was prepared using Ru (dpp) 3 Cl 2 @ DMSN, a polydimethylsiloxane PDMS matrix, and a PDMS curing agent.
3. The method of claim 2, wherein the preparing dendritic mesoporous silica nanoparticles DMSN using cetyltrimethylammonium p-toluenesulfonate, triethanolamine and deionized water comprises: Mixing hexadecyl trimethyl ammonium p-toluenesulfonate with a first mass and triethanolamine with a second mass with deionized water with a first capacity according to a first preparation proportion, and stirring for a first duration at a first temperature to obtain a first solution; Stirring and mixing tetraethyl silicate with a second capacity and the first solution at a second temperature for a second period of time to obtain DMSN precursor; placing the pre-treated DMSN precursor into a sintering device, and increasing the temperature of the sintering device to a first target temperature at a preset heating rate; and maintaining the first target temperature until the third time period is met, and then canceling heating and cooling to room temperature to obtain the prepared DMSN.
4. The method according to claim 2, wherein the preparing dendritic mesoporous silica nanoparticles Ru (dpp) 3 Cl 2 @ DMSN uniformly loaded with Ru (dpp) 3 Cl 2 using a silica suspension prepared based on the DMSN and an aqueous sodium hydroxide solution and a mixed solution of Ru (dpp) 3 Cl 2 and an absolute ethanol solution and washing with ethanol comprises: Mixing DMSN of a third mass and a third volume of sodium hydroxide aqueous solution according to a second preparation ratio and continuously stirring for a fourth time to obtain a silicon dioxide suspension; mixing Ru (dpp) 3 Cl 2 with fourth capacity of absolute ethyl alcohol solution according to a third preparation proportion to obtain a second solution; The second solution was added to the silica suspension and, after a fifth period of continuous stirring, vacuum filtration separation was performed to give Ru (dpp) 3 Cl 2 @ DMSN.
5. The method of claim 2, wherein the preparing the oxygen sensitive film using Ru (dpp) 3 Cl 2 @ DMSN, a polydimethylsiloxane PDMS matrix, and a PDMS curative comprises: According to a fourth preparation proportion, mixing Ru (dpp) 3 Cl 2 @ DMSN of the fifth mass and a PDMS matrix of the sixth mass in a toluene solution of a fifth capacity to a transparent state to obtain a third solution; after the third solution is subjected to ultrasonic treatment for a sixth time period, continuously magnetically stirring at room temperature for a seventh time period according to a first preset rotating speed to obtain an initial composite material; Adding a seventh mass of PDMS curing agent to the composite material and degassing in a vacuum environment for an eighth period of time to obtain a target composite material; Continuously rotating the target composite material for a ninth time period according to a second preset rotating speed, and continuously rotating the target composite material for a tenth time period according to a third preset rotating speed; And spin-coating the composite material subjected to the tenth time period on a glass slide, and curing the composite material at a second target temperature for a target time period to obtain the oxygen sensitive film.
6. The method of claim 1, wherein the imaging path comprises a kohler illumination module and a polarized beam splitting module; The imaging path alternately irradiates a plurality of types of excitation light on the tissue to be detected according to a target interval, and the imaging path comprises the following steps: And alternately flashing a plurality of types of excitation light according to a target interval, wherein each type of excitation light sequentially passes through the Kohler illumination module and the polarized beam splitting module and irradiates on the tissue to be detected.
7. The method of claim 1, wherein the plurality of types of excitation light comprises red light, infrared light, and blue light, wherein the different cameras comprise at least a first camera and a second camera, wherein the first excitation light signal comprises an oxygen phosphorescence signal excited by the blue light based on an oxygen quenching effect, wherein the second excitation light signal comprises a calcium fluorescence signal excited by the blue light based on a calcium fluorescence characteristic of the specific tissue cells, wherein the reflected light signal comprises a reflected red light signal and a reflected infrared light signal of blood oxygen in the tissue to be detected; The method for continuously collecting each frame of first excitation light signals on the oxygen sensitive film, each frame of second excitation light signals of the specific tissue cells and each frame of reflected light signals of blood oxygen in the tissue to be detected by using different cameras according to the target interval comprises the following steps: According to the target interval, continuously acquiring oxygen phosphorescence signals of each frame on the oxygen sensitive film by using the first camera, continuously acquiring calcium fluorescence signals of each frame of the specific tissue cells by using the second camera, and continuously acquiring reflection red light signals or reflection infrared light signals of each frame of blood oxygen in the tissue to be detected by using the first camera.
8. The method of claim 1, wherein the tissue to be detected comprises at least brain tissue, gastrointestinal tissue, tumor area tissue, and wherein the tissue to be detected has a predetermined pathological condition.
9. An imaging system, comprising an oxygen sensitive film and an imaging unit, the imaging unit comprising an illumination module, an acquisition module, and a generation module; the oxygen sensitive film is used for being arranged on a tissue to be detected of an object to be detected, wherein the tissue to be detected is provided with specific histiocyte sensitive to calcium signals; The illumination module is used for alternately irradiating a plurality of types of excitation light on the tissue to be detected according to a target interval through an imaging passage; The acquisition module is used for respectively and continuously acquiring each frame of first excitation light signals on the oxygen sensitive film, each frame of second excitation light signals of the specific tissue cells and each frame of reflected light signals of blood oxygen in the tissue to be detected by utilizing different cameras according to the target interval; The generation module is used for generating a tissue oxygen partial pressure image of the tissue to be detected according to the first excitation light signals of each frame which is continuously collected, generating a calcium signal image of the tissue to be detected according to the second excitation light signals of each frame which is continuously collected, and generating a blood oxygen signal image of the tissue to be detected according to the reflected light signals of each frame which is continuously collected.
10. A computer readable storage medium, on which a computer program is stored, characterized in that the program, when being executed by a processor, implements the steps of the method according to any one of claims 1 to 8.
11. A computer device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, characterized in that the processor implements the steps of the method according to any one of claims 1 to 8 when the program is executed.

Description

Imaging method, imaging system, storage medium and computing device Technical Field The present application relates to the field of biomedical imaging technology, and in particular, to an imaging method, system, storage medium and computing device. Background Proper functioning of various internal tissues of an organism often requires coordination of precise physiological activities within the tissues. In order to deeply reveal the influence of different tissue diseases on internal physiological tissues, when tissue diseases occur, the change condition of physiological activities in the physiological tissues needs to be acquired and intuitively quantified, and the change condition usually needs to be acquired by relying on an imaging mode. For example, proper functioning of brain tissue requires delicate coordination between neural activity, blood flow supply and energy metabolism. In order to deeply reveal the pathological mechanism of brain diseases such as epilepsy, stroke and the like, a complete causal chain among the three needs to be observed. The causal chain is usually observed by imaging physiological activities in brain tissue. Because of the generation and change of diseases, the influence on physiological activities has a linkage effect, and the prior art usually images different physiological activities by means of different imaging devices respectively, and then comprehensively analyzes based on imaging results of each time so as to realize the acquisition of the physiological activity change condition inside physiological tissues. However, this approach has significant bottlenecks in terms of integration, spatial-temporal resolution, and long-term stability, which are relatively large. Disclosure of Invention In view of this, the present application provides an imaging method, system, storage medium and computing device that significantly improve the integration, spatiotemporal resolution and long-term stability of tissue imaging by simultaneously performing tissue imaging in multiple modalities. Specifically, the application is realized by the following technical scheme: in a first aspect, an embodiment of the present application provides an imaging method, including: installing an oxygen sensitive film with an oxygen sensitive probe prepared in advance on a tissue to be detected of an object to be detected, wherein the tissue to be detected comprises specific tissue cells with calcium signal sensitivity; alternately irradiating a plurality of types of excitation light on the tissue to be detected according to a target interval through an imaging passage; according to the target interval, respectively and continuously acquiring each frame of first excitation light signals on the oxygen sensitive film, each frame of second excitation light signals of the specific tissue cells and each frame of reflected light signals of blood oxygen in the tissue to be detected by using different cameras; Generating a tissue oxygen partial pressure image of the tissue to be detected according to the first excitation light signals of each frame which is continuously collected, generating a calcium signal image of the tissue to be detected according to the second excitation light signals of each frame which is continuously collected, and generating a blood oxygen signal image of the tissue to be detected according to the reflected light signals of each frame which is continuously collected. In a second aspect, embodiments of the present application further provide an imaging system, the system including an oxygen-sensitive film and an imaging unit, the imaging unit including an illumination module, an acquisition module, and a generation module; the oxygen sensitive film is used for being arranged on a tissue to be detected of an object to be detected, wherein the tissue to be detected is provided with specific histiocyte sensitive to calcium signals; The illumination module is used for alternately irradiating a plurality of types of excitation light on the tissue to be detected according to a target interval through an imaging passage; The acquisition module is used for respectively and continuously acquiring each frame of first excitation light signals on the oxygen sensitive film, each frame of second excitation light signals of the specific tissue cells and each frame of reflected light signals of blood oxygen in the tissue to be detected by utilizing different cameras according to the target interval; The generation module is used for generating a tissue oxygen partial pressure image of the tissue to be detected according to the first excitation light signals of each frame which is continuously collected, generating a calcium signal image of the tissue to be detected according to the second excitation light signals of each frame which is continuously collected, and generating a blood oxygen signal image of the tissue to be detected according to the reflected light signals of each frame which is continuous