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CN-121971119-A - System and method for sampling gastric juice and intestinal juice of living yak capable of being positioned in segmented mode

CN121971119ACN 121971119 ACN121971119 ACN 121971119ACN-121971119-A

Abstract

The invention relates to the technical field of animal physiology research, and discloses a yak living body gastric juice and intestinal juice sampling system and method capable of achieving segmented positioning. According to the method, real-time navigation is performed through the sensor, the positioning units are sequentially controlled to anchor at different target positions of the yak digestive tracts, and pure digestive juice samples are respectively extracted. The invention solves the problems of inaccurate positioning, large wound and easy sample pollution in the prior art, realizes noninvasive, accurate and repeatable acquisition of the microbial sample of the gastrointestinal tract of the living yak, and is particularly suitable for research of gastrointestinal microbiology.

Inventors

  • DING CHENGXIANG
  • ZHANG JUN
  • WANG GUOWEN

Assignees

  • 青海大学

Dates

Publication Date
20260505
Application Date
20260203

Claims (10)

  1. 1. But yak live body gastric juice and intestinal juice sampling system of segmentation location, its characterized in that includes: the negative pressure sampling unit (100) comprises a sampling catheter (110), a valve (120) and a negative pressure source (130), wherein the negative pressure sampling unit (100) is used for extracting and conveying a liquid sample of a target part to the outside of the body; The device comprises a sampling catheter (110), a plurality of positioning units (200), a plurality of positioning units and a plurality of control units, wherein the positioning units are arranged on the outer wall of the sampling catheter (110) and are axially arranged at intervals along the sampling catheter (110), each positioning unit (200) comprises an air bag, a connecting rod, a control air circuit and a miniature air pump, and the positioning units (200) are used for expanding at a target part to anchor the sampling catheter (110); The physiological parameter sensing unit (300) is arranged on the outer wall of the input end of the sampling catheter (110), and the physiological parameter sensing unit (300) is used for detecting physiological parameters of the environment where the input end of the sampling catheter (110) is positioned; The control unit (400) is in communication connection with the physiological parameter sensing unit (300), the positioning unit (200) and the negative pressure sampling unit (100), and the control unit (400) is used for determining whether the input end of the sampling catheter (110) reaches a target position or not and controlling the positioning unit to anchor; And the multi-connected independent sample collection device (500) is connected with the negative pressure sampling unit (100), and the multi-connected independent sample collection device (500) is used for collecting liquid samples collected from different target sites.
  2. 2. The segmented positioning system for gastric juice and intestinal juice of the living yak according to claim 1, wherein one side of a control air path in the positioning unit (200) is connected with an air bag in a penetrating way, the other side of the control air path is connected with a micro air pump, and the micro air pump is in communication connection with the control unit (400).
  3. 3. The sectionally positionable system for gastric and intestinal fluid sampling of a living yak of claim 1, wherein the multi-piece independent sample collection device (500) comprises a plurality of sample collection vials (510) and a multi-way valve (520), wherein a common port of the multi-way valve (520) is connected to the output end of the sampling tube (110), a selection port is connected to each of the sample collection vials (510) through a separate line, the multi-way valve (520) directs the liquid samples from different target sites output by the sampling tube (110) to different sample collection vials (510), and the multi-way valve (520) is in communication with the control unit (400).
  4. 4. The system for sampling gastric juice and intestinal juice of a living yak body capable of being positioned in segments according to claim 1, wherein a valve (120) in the negative pressure sampling unit (100) is arranged at the front end of the sampling catheter (110) and used for controlling the opening and closing of the sampling catheter (110), a negative pressure source (130) is arranged between the tail end of the sampling catheter (110) and the multiple independent sample collecting devices (500) and used for generating suction force and collecting liquid samples of target parts, and the valve (120) and the negative pressure source (130) are in communication connection with the control unit (400).
  5. 5. The sectionally positionable system for sampling gastric juice and intestinal juice of a living yak of claim 1, wherein the physiological parameter sensing unit (300) comprises a pH sensor and a pressure sensor.
  6. 6. The segmented positioning system for sampling gastric juice and intestinal juice of a living yak according to claim 1, wherein physiological parameter threshold values or characteristic waveform data of different parts of the digestive tract of the yak are pre-stored in the control unit (400), and the control unit (400) is configured to determine whether the input end of the sampling catheter (110) reaches the target part based on the pre-stored data and the data detected by the physiological parameter sensing unit (300).
  7. 7. The segmented positioning system for gastric juice and intestinal juice of the living yak according to claim 1, further comprising a display unit connected with the control unit (400) for displaying the detection data of the physiological parameter sensing unit (300) and the state of the positioning unit (200) in real time.
  8. 8. A segmented positioning system for sampling gastric juice and intestinal juice of a living yak as claimed in claim 3 wherein said sample collection bottle (510) is pre-filled with anaerobic preservation solution for microbiome study.
  9. 9. The segmented positioning system for gastric juice and intestinal juice of the living yak of claim 2, wherein the two positioning units are a first positioning unit (210) arranged near the front end of the sampling catheter (110) and a second positioning unit (220) arranged behind the first positioning unit, the first positioning unit (210) comprises a first air bag (211), a first connecting rod (212), a first control air channel (213) and a first micro air pump (214), and the second positioning unit (220) comprises a second air bag (221), a second connecting rod (222), a second control air channel (223) and a second micro air pump (224).
  10. 10. A method of using the sectionally positionable yak living gastric and intestinal fluid sampling system of any of claims 1-9, comprising the steps of: S1, inserting and advancing the sampling catheter (110) through a yak oral cavity; S2, monitoring physiological parameters detected by the physiological parameter sensing unit (300) based on the control unit (400) in the pushing process, and controlling a first micro air pump (214) in a first positioning unit (210) to perform inflation treatment on a first air bag (211) based on the control unit (400) until the sampling catheter is anchored when the parameters indicate that the first target position is reached; S3, starting a negative pressure source (130) in a negative pressure sampling unit (100) based on the control unit (400), extracting a liquid sample of a first target part, and conveying the liquid sample to a first collecting bottle in a multi-connected independent sample collecting device (500) based on the sampling catheter (110); S4, controlling a first micro air pump (214) in a first positioning unit (210) to carry out air extraction treatment on the first air bag (211) based on the control unit (400), releasing anchoring, and continuing to push the sampling catheter (110); S5, when the physiological parameter detected by the physiological parameter sensing unit (300) indicates that the physiological parameter reaches a second target position, a second micro air pump (224) in a second positioning unit (220) is controlled based on a control unit (400) to perform inflation treatment on a second air bag (221) until the sampling catheter (110) is anchored; S6, starting a negative pressure source (130) in the negative pressure sampling unit (100) based on the control unit (400), extracting a liquid sample of a second target part, conveying the liquid sample to a second collecting bottle in the multi-connected independent sample collecting device (500) based on the sampling catheter (110), controlling a second micro air pump (224) in the second positioning unit (220) to carry out air extraction treatment on a second air bag (221), releasing anchoring, and withdrawing the sampling catheter after sampling is completed.

Description

System and method for sampling gastric juice and intestinal juice of living yak capable of being positioned in segmented mode Technical Field The invention relates to the technical field of animal physiology research, in particular to a yak living body gastric juice and intestinal juice sampling system and method capable of achieving segmented positioning. Background The yak is taken as an important livestock species special for alpine regions, and the gastrointestinal microbial community plays a vital role in nutrition digestion, health and adaptability. The research on the microbial compositions of gastric juice and intestinal juice is of great significance for optimizing feeding, disease prevention and variety breeding. Currently, the methods for obtaining gastrointestinal fluids from ruminants are mainly (1) fistulae, where a permanent fistula is surgically placed in the stomach or intestinal wall. The method is large in wound, easy to infect, affects animal welfare and normal physiology, and is not suitable for frequent sampling of healthy groups. (2) oral/nasal cannula sampling by inserting a long catheter into the stomach. For multi-gastric yaks, it is difficult to accurately distinguish between rumen, reticulum, valve stomach and abomasum, and it is less reliable to access specific sections of the small intestine. The sampling process relies on experience, is not accurately positioned, and the sampling tube is easily displaced along with peristaltic movement in the digestive tract, so that sampling failure or sample pollution is caused. (3) The method has the advantages that the capsule can be swallowed, the recovery in yaks is difficult, the movement of the capsule is uncontrollable, the capsule can not be sampled at a designated position, and the cost is high. The prior art lacks a sampling means that can be used to perform living, minimally invasive, repeatable and accurate positioning of a yak to a particular stomach or intestinal tract. Accordingly, it is desirable to provide a sampling system and method that can solve the above-mentioned problems. Disclosure of Invention In view of the above, the invention provides a system and a method for sampling gastric juice and intestinal juice of a living yak capable of being positioned in a sectionalized manner, which aim to solve the problems that the current technique for sampling the living yak cannot accurately position and stably maintain the living yak in a specific section in the digestive tract, and cannot acquire gastric juice and intestinal juice samples sectionally in one operation without pollution. In one aspect, the invention provides a yak living body gastric juice and intestinal juice sampling system capable of being positioned in a segmented mode, which comprises the following steps: The negative pressure sampling unit comprises a sampling catheter, a valve and a negative pressure source, wherein the negative pressure sampling unit is used for extracting and conveying a liquid sample of a target part to the outside of the body, the tube body of the sampling catheter is made of flexible materials with good biocompatibility, the outer diameter and the bending rigidity are specially designed according to the anatomical dimensions of the esophagus and the intestinal tract of an adult yak so as to ensure smooth insertion and no damage, and the inner wall of the sampling catheter is smooth so as to reduce the adsorption and the residue in the sample conveying process; The device comprises a sampling catheter, a plurality of positioning units, a plurality of control air channels, a plurality of air inlets and a plurality of air outlets, wherein the positioning units are arranged on the outer wall of the sampling catheter and are axially arranged at intervals along the sampling catheter; The physiological parameter sensing unit is arranged on the outer wall of the input end of the sampling catheter and is used for detecting physiological parameters of the environment where the input end of the sampling catheter is positioned; The control unit is in communication connection with the physiological parameter sensing unit, the positioning unit and the negative pressure sampling unit, and is used for determining whether the input end of the sampling catheter reaches a target position or not and controlling the positioning unit to anchor; And the multi-connection independent sample collection device is connected with the negative pressure sampling unit and is used for collecting liquid samples collected from different target positions. Further preferably, one side of a control air path in the positioning unit is connected with the air bag in a penetrating way, the other side of the control air path is connected with the miniature air pump, and the miniature air pump is in communication connection with the control unit. Further preferably, the multi-connected independent sample collection device comprises a plurality of sample collection bottles and