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CN-121971195-A - Method for constructing liver injury animal model

CN121971195ACN 121971195 ACN121971195 ACN 121971195ACN-121971195-A

Abstract

The invention discloses a method for constructing a liver injury animal model, which comprises the steps of (a) providing a model animal, completely anesthetizing the model animal, (b) taking out the liver of the model animal, confirming the surgical excision proportion, and (c) fixing the liver part to be excised by using a surgical suture line, and performing liver excision. The method can construct the liver injury mouse model more efficiently, and can meet the liver injury proportion required by different liver injury models.

Inventors

  • GU YONGDONG
  • ZHOU LU
  • Kui Fuguang
  • ZHANG JINSONG
  • SHA SHIPENG
  • QU LINGCHEN

Assignees

  • 益诺思生物技术南通有限公司

Dates

Publication Date
20260505
Application Date
20251226

Claims (10)

  1. 1. A method of constructing an animal model of liver injury, the method comprising: (a) Providing a model animal, and fully anesthetizing the model animal; (b) Removing the liver of the model animal, confirming the surgical resection rate, and (C) The liver site to be resected is fixed using a surgical suture, and liver resection is performed.
  2. 2. The method of claim 1, wherein the model animal is a mouse, preferably a C57BL/6 mouse.
  3. 3. The method of claim 1 or 2, wherein the ablation rate in step (b) is not less than 30%, preferably not less than 30% -90%, more preferably 30%, 40%, 50%, 60%, 70%, 80% or 90%.
  4. 4. The method of any one of claims 1-3, wherein the location of the excision is selected from one or more of the group consisting of a left lateral lobe, a left medial lobe, and a right medial lobe.
  5. 5. The method of claim 4, wherein the resected locations are left lateral liver lobe, left medial liver lobe and right medial liver lobe, and wherein the left lateral liver lobe, left medial liver lobe and right medial liver lobe are resected 80% -95%, preferably 90% each.
  6. 6. The method of any one of claims 1-5, wherein the fixed location in step (c) is near the resection site.
  7. 7. The method of claim 6, wherein the fixed location is 1 to 5mm below the ablation site, preferably the fixed location is 2 to 3mm below the ablation site.
  8. 8. A method according to any one of claims 1 to 7, wherein said resecting does not comprise resecting the gallbladder of said model animal.
  9. 9. An animal model for liver injury, which is characterized in that, the liver injury animal model constructed by the method of any one of claims 1-8.
  10. 10. Use of the liver injury animal model of claim 9 in a medicament for assessing the level of regeneration following a liver cell transplant.

Description

Method for constructing liver injury animal model Technical Field The present invention relates to the field of biotechnology. In particular, the invention relates to a method for constructing an animal model of liver injury. Background Liver diseases are among the most frequently occurring diseases in humans. The clinical treatment of liver diseases is mainly liver transplantation operation, and the liver transplantation operation also has a plurality of problems to be solved to improve the regeneration and proliferation level of liver cells after transplantation. Compared with the transplantation of liver tissue, the transplantation of liver cells is more feasible, has low cost and is easy to implement. The preclinical construction of animal models of major human diseases is a necessary tool for the medical research of transformation of various major diseases, and the establishment of animal models of liver injury is a key technical platform for researching liver cell transplantation and regeneration. At present, several methods for constructing animal models of liver injury have been reported. For example, in chinese patent application number 201510153724.4, an inducible specific liver injury mouse model is disclosed. However, the model is performed by using a chemical substance induction method, the operation is not convenient enough, and the cost is high. Therefore, the existing liver injury mouse model needs to be further optimized, and a method for constructing the liver injury mouse model more efficiently is urgently needed in the field, so that convenient support is provided for various subsequent novel liver disease animal models. Disclosure of Invention The invention aims to provide a method for constructing a liver injury mouse model more efficiently, which provides technical support for various follow-up novel liver disease animal models. The construction method provided by the invention can obtain a liver injury mouse model which is constructed more efficiently, and the construction method adopts a specific site operation cutting method, so that the liver injury proportion required by different liver injury models can be met. In one aspect of the present invention, there is provided a method of constructing an animal model of liver injury, the method comprising: (a) Providing a model animal, and fully anesthetizing the model animal; (b) Removing the liver of the model animal, confirming the surgical resection rate, and (C) The liver site to be resected is fixed using a surgical suture, and liver resection is performed. In some embodiments, the model animal is a mouse. In some embodiments, the model animal is a C57BL/6 mouse. In some embodiments, the cut-out ratio in step (b) is not less than 30%. In some embodiments, the cut-out ratio in step (b) is not less than 70%. In some embodiments, the excision fraction in step (b) is 30%, 40%, 50%, 60%, 70%, 80% or 90%. In some embodiments, the location of the excision is selected from one or more of the group consisting of a left lateral lobe, a left medial lobe, and a right medial lobe. In some embodiments, the resected locations are a left lateral lobe, a left medial lobe and a right medial lobe, each resected 80% -95%; preferably 90%. In some embodiments, wherein the fixed location in step (c) is near the resection site. In some embodiments, the fixed location is 1 to 5mm below the resection site. In some embodiments, the fixed location is 2 to 3mm below the resection site. In some embodiments, the resecting does not comprise resecting the gallbladder of the model animal. In one aspect of the present invention, there is provided an animal model of liver injury constructed by the method of the present invention. In one aspect of the invention, there is provided the use of an animal model of liver injury according to the invention in a medicament for assessing the level of regeneration following a liver cell transplant. On the basis of conforming to the common knowledge in the field, the above preferred conditions can be arbitrarily combined to obtain the preferred examples of the invention. The reagents and materials used in the present invention are commercially available. The invention has the positive progress effects that: The liver injury mouse model obtained by the construction method provides a feasible animal model support for liver transplantation research, has important theoretical significance and application prospect for human research such as liver cell transplantation and liver regeneration, and provides technical support for a humanized animal model platform for various liver disease model researches. Drawings FIG. 1 is a liver map of C57BL/6 mice prior to surgical excision. FIG. 2 is a liver map of C57BL/6 mice after surgical excision of 30%. FIG. 3 is a liver map of C57BL/6 mice after surgical excision of 70%. FIG. 4 is a graph showing the change in ALB in serum after surgical excision of 30% from C57BL/6 mice. FIG. 5 is a graph sho