CN-121971312-A - Silk fibroin penetration-promoting nanoparticle, preparation method, functional cosmetic carrier and application

CN121971312ACN 121971312 ACN121971312 ACN 121971312ACN-121971312-A

Abstract

The invention belongs to the technical field of cosmetic carriers, and particularly discloses a silk fibroin penetration-promoting nanoparticle, a preparation method, a functional cosmetic carrier and application. The permeation promoting nanoparticle comprises a compact inner core region, a lipid limiting interface region, an ion bridging transition region and a composite hydration outer layer region which are sequentially arranged from inside to outside, wherein the compact inner core region comprises silk fibroin, polyalcohol and polyhydroxy acid ester, the lipid limiting interface region comprises phospholipid, ceramide, cholesterol and fatty acid ester, the ion bridging transition region comprises bridging components and arginine, and the composite hydration outer layer region comprises trehalose, hyaluronic acid oligosaccharide, sodium pyroglutamate, ectoin and zwitterionic surface components. The technical proposal has better particle stability, penetration promoting capability, skin retention capability and formula suitability.

Inventors

CHEN BINGGUO
HUANG WENSHU
Zang Demin
WENG HEMING
WU XIAOYE
WEN DAN

Assignees

杭州达维先医药科技有限公司
江西续创医学科技有限公司

Dates

Publication Date: 20260505
Application Date: 20260407

Claims (10)

1. The silk fibroin infiltration promoting nanoparticle is characterized by comprising a compact inner core region, a lipid limiting interface region, an ion bridging transition region and a composite hydration outer layer region which are sequentially arranged from inside to outside; The dense inner core region comprises silk fibroin, polyol and polyhydroxyalkanoate; The lipid limiting interface region is coated outside the compact inner core region, and comprises phospholipid, ceramide, cholesterol and fatty acid ester; The ion bridging transition zone is arranged outside the lipid limiting interface zone and comprises a bridging component and arginine; The composite hydration outer layer region is arranged outside the ion bridging transition region and comprises trehalose, hyaluronic acid oligosaccharide, sodium pyroglutamate, ectoin and zwitterionic surface components; The silk fibroin permeation promotion nanoparticle comprises, by mass, 38-58 parts of silk fibroin, 6-14 parts of polyalcohol, 2-7 parts of polyhydroxyalkanoate, 6-14 parts of phospholipid, 0.8-3.5 parts of ceramide, 0.6-2.8 parts of cholesterol, 3-8 parts of fatty acid ester, 0.2-1.8 parts of bridging component, 0.5-3.5 parts of arginine, 3-10 parts of trehalose, 0.5-4 parts of hyaluronic acid oligosaccharide, 1.5-5 parts of sodium pyroglutamate, 0.5-3 parts of ectoin and 0.2-1.5 parts of zwitterionic surface component; The mass ratio of the total mass of the compact core region to the total mass of the lipid limiting interface region is 100:18-52, the mass ratio of the phospholipid, the ceramide, the cholesterol and the fatty acid ester is 10-20:1-4:1-3:4-10, the mass ratio of the bridging component to the arginine is 1:1.2-4.5, and the mass ratio of the trehalose, the hyaluronic acid oligosaccharide, the sodium pyroglutamate and the ectoin is 8-20:1-6:3-10:1-5.
2. The silk fibroin infiltration-promoting nanoparticle of claim 1, wherein the bridging component is selected from one or both of sodium phytate, zinc gluconate, and choline citrate; The zwitterionic surface component is selected from one or two of cocamidopropyl betaine, lauryl betaine and hydroxysulfobetaine.
3. The silk fibroin infiltration-promoting nanoparticle of claim 1, wherein the polyol is selected from two of glycerol, 1, 3-propanediol, and butanediol; the polyhydroxy acid ester is selected from one or two of gluconolactone, sodium lactate and triethyl citrate; The mass ratio of the polyol to the polyhydroxy acid ester is 2.5-5.5:1.
4. The silk fibroin infiltration-promoting nanoparticle according to claim 1, wherein the phospholipids are hydrogenated lecithin and phosphatidylcholine, the mass ratio of the hydrogenated lecithin to the phosphatidylcholine is 1:0.6-2.5, the ceramide is ceramide NP and ceramide AP, and the mass ratio of the total amount of the ceramide NP and the ceramide AP to cholesterol is 1:0.8-2.2.
5. The silk fibroin penetration-promoting nanoparticle according to claim 1, wherein the mass ratio of trehalose to hyaluronic acid oligosaccharides is 3-12:1, the mass ratio of sodium pyroglutamate to exendin is 1.5-4.5:1, and the mass ratio of the total amount of trehalose, hyaluronic acid oligosaccharides, sodium pyroglutamate and exendin to zwitterionic surface components is 100:2-12.
6. A method for preparing the silk fibroin infiltration-promoting nanoparticle according to any one of claims 1 to 5, comprising the steps of: s1, dissolving degummed silk fibroin in a neutral salt solution or an ionic liquid solution, and obtaining a silk fibroin aqueous solution after dialysis and desalting; s2, adding polyalcohol and polyhydroxy acid ester into the silk fibroin aqueous solution, mixing, and adding an alcohol precipitation agent to form a compact kernel area dispersion system; S3, preparing phospholipid, ceramide, cholesterol and fatty acid ester into a lipid mixed phase, and adding the lipid mixed phase into the compact kernel area dispersion system to form a lipid limiting interface area; s4, adding a bridging component and arginine into the system obtained in the step S3 to form an ion bridging transition zone; s5, adding trehalose, hyaluronic acid oligosaccharide, sodium pyroglutamate, ectoin and zwitterionic surface components into the system obtained in the S4 to form a composite hydration outer layer region; s6, one or more treatments of dialysis, ultrafiltration and centrifugal washing and/or freeze-drying treatment are carried out, so that the silk fibroin permeation promotion nanoparticle dispersion liquid or powder is obtained.
7. The preparation method of claim 6, wherein the alcohol precipitation agent in the step S2 is ethanol, isopropanol or a combination of the ethanol and the isopropanol, and the alcohol precipitation agent is added in two stages, wherein the addition amount in the first stage is 20% -50% of the total addition amount, and the addition amount in the second stage is 50% -80% of the total addition amount; in the step S3, after pre-mixing the phospholipid and the ceramide, adding cholesterol and fatty acid ester; in the step S4, the bridging component is added firstly, and arginine is added later; in the step S5, the trehalose and the hyaluronic acid oligosaccharide are added in two times, and the sodium pyroglutamate, the ectoin and the zwitterionic surface component are added in a mixed mode after the trehalose and the hyaluronic acid oligosaccharide are added in the second time.
8. A functional cosmetic carrier, characterized in that the functional cosmetic carrier comprises a cosmetic matrix, an active component and the silk fibroin penetration-promoting nanoparticles of any one of claims 1-5; The cosmetic matrix comprises an aqueous phase component, a moisturizing component, a thickening component, a preservative component, a chelating component and a pH adjusting component; The moisturizing component is at least one of glycerol, butanediol, 1, 3-propanediol and dipropylene glycol; The thickening component is selected from at least one of xanthan gum, carbomer, acrylic polymer and cellulose derivative; the preservative component is at least one selected from phenoxyethanol, ethylhexyl glycerol, potassium sorbate and 1, 2-hexanediol; The chelating component is at least one selected from disodium ethylenediamine tetraacetate, sodium phytate and sodium gluconate; The pH regulating component is at least one of arginine, sodium hydroxide, citric acid and sodium citrate; the content of the silk fibroin penetration-promoting nanoparticles in the functional cosmetic carrier is 0.1% -12% in terms of solid matters.
9. The functional cosmetic carrier of claim 8, wherein the active component is selected from at least one of nicotinamide, panthenol, tranexamic acid, ceramide, oligopeptide, centella asiatica extract, a fermentation product filtrate of saccharomyces cerevisiae, ascorbyl tetrahexyldecanol, and coenzyme Q10; when the active component comprises a water-soluble active component and an oil-soluble active component, the water-soluble active component is added to the aqueous phase component of the cosmetic matrix and the oil-soluble active component is added to the oil phase component of the cosmetic matrix, the silk fibroin permeation enhancing nanoparticles being used to support the active component; the mass ratio of the active component to the silk fibroin infiltration promoting nanoparticle is 1:10-15:1 in terms of solid matters.
10. The use of the functional cosmetic carrier according to claim 8 or 9 in a cosmetic for external use, wherein the type of the cosmetic for external use is one of an essence, an emulsion, a cream, a gel, a spray liquid, a mask liquid and a lyophilized powder complex solution; When the functional cosmetic carrier is prepared, the silk fibroin permeation promoting nano particles are added into a water phase component, then a moisturizing component, a chelating component and an active component are added, and then a thickening component, a preservative component and a pH adjusting component are added.

Description

Silk fibroin penetration-promoting nanoparticle, preparation method, functional cosmetic carrier and application Technical Field The invention belongs to the technical field of cosmetic carriers, and particularly discloses a silk fibroin penetration-promoting nanoparticle, a preparation method, a functional cosmetic carrier and application. Background Silk fibroin has been a natural polymer material with wide sources, good biocompatibility and degradability, and has been attracting attention in biomedical materials, drug delivery and skin care fields in recent years. Especially in the field of functional cosmetics, silk fibroin has been increasingly used for constructing a variety of novel delivery vehicles due to its superior film forming properties, moisturizing properties, skin-friendly properties, and entrapment adaptability to active ingredients. Meanwhile, as the demands of consumers on the efficacy, safety and mildness of cosmetics are continuously increased, the development of a carrier system with both stable entrapment and efficient delivery capabilities by utilizing natural biological materials has become one of the important directions of the technical development of functional cosmetics. At present, the delivery route of active ingredients in functional cosmetics mainly comprises liposome, emulsion, microemulsion, polymer nanoparticles, inorganic porous carrier, surfactant-assisted penetration promoting system and the like. Such technical routes generally promote the use of cosmetics by improving the dispersibility of the active ingredient, enhancing the moisturizing effect of the stratum corneum, extending the residence time on the skin surface or promoting migration of the ingredient to the shallow layers of the skin by means of small particle size effects. However, the prior art still has certain limitations in practical application. For example, some carrier systems have complex preparation process, high requirements on process conditions and equipment, are not beneficial to large-scale stable production, some permeation promotion systems rely on alcohols, surfactants or chemical permeation promoters to realize permeation enhancement, are easy to stimulate skin barriers, and some nano carriers have good entrapment capacity, but have the problems of insufficient stability, easy leakage of active ingredients, obvious particle diameter change in the storage process and the like. In addition, certain carrier materials have poor compatibility with cosmetic systems, and it is difficult to balance safety, mildness, loading efficiency and penetration enhancing efficiency, so that the application in functional cosmetics is still limited. Therefore, it is necessary to explore a novel delivery system more suitable for skin care needs to meet the comprehensive requirements of the functional cosmetic field on safety, effectiveness and practicality, aiming at the defects of the existing functional cosmetic carrier in terms of mildness, penetration promotion, stable entrapment and application suitability. Disclosure of Invention Aiming at the problems, the invention aims to provide a silk fibroin infiltration promotion nanoparticle, which comprises a compact inner core region, a lipid limiting interface region, an ion bridging transition region and a composite hydration outer layer region which are sequentially arranged from inside to outside; The dense inner core region comprises silk fibroin, polyol and polyhydroxyalkanoate; The lipid limiting interface region is coated outside the compact inner core region, and comprises phospholipid, ceramide, cholesterol and fatty acid ester; The ion bridging transition zone is arranged outside the lipid limiting interface zone and comprises a bridging component and arginine; the composite hydration outer layer region is arranged outside the ion bridging transition region, and comprises trehalose, hyaluronic acid oligosaccharide, sodium pyroglutamate, ectoin and zwitterionic surface components. According to the preferred technical scheme, the silk fibroin permeation promotion nanoparticle comprises, by mass, 38-58 parts of silk fibroin, 6-14 parts of polyol, 2-7 parts of polyhydroxy acid ester, 6-14 parts of phospholipid, 0.8-3.5 parts of ceramide, 0.6-2.8 parts of cholesterol, 3-8 parts of fatty acid ester, 0.2-1.8 parts of bridging component, 0.5-3.5 parts of arginine, 3-10 parts of trehalose, 0.5-4 parts of hyaluronic acid oligosaccharide, 1.5-5 parts of sodium pyroglutamate, 0.5-3 parts of ectoin and 0.2-1.5 parts of zwitterionic surface component; As a preferable technical scheme, the mass ratio of the total mass of the compact inner core region to the total mass of the lipid limiting interface region is 100:18-52, the mass ratio of the phospholipid, the ceramide, the cholesterol and the fatty acid ester is 10-20:1-4:1-3:4-10, the mass ratio of the bridging component to the arginine is 1:1.2-4.5, and the mass ratio of the trehalose, the hyaluronic acid oligosacc