CN-121971327-A - Hair loss preventing essence containing plant source exosomes and preparation method thereof

CN121971327ACN 121971327 ACN121971327 ACN 121971327ACN-121971327-A

Abstract

The invention discloses an anti-hair loss essence containing plant source exosomes and a preparation method thereof, wherein the anti-hair loss essence comprises, by mass, 5-10% of plant source exosomes, 3-5% of hair growth nutrients, 1-3% of hair follicle blood circulation activators, 2-6% of penetration promoters, 1.5-2% of oil control bacteriostats, 0.45-1% of preservatives and 77.5-82.75% of solvents, the plant source exosomes are prepared by mixing a cacumen biotae exosomes solution, a polygonum multiflorum root exosomes solution, a black ginseng root exosomes solution and a herba inulae exocrine solution according to a mass ratio of 3-5:1.5:1.5:4-6, the plant source exosomes are used as core efficacy components of the anti-hair loss essence, and are matched with assistants such as hair growth nutrients, hair follicle blood circulation activators, promoters and the like, so that the delivery efficiency of plant active nutrient components and the absorption efficiency of scalp can be effectively improved, and the synergistic effect among the plant source exosomes is further, hair growth promotion and hair loss prevention.

Inventors

LI CHENGXIONG
LAN YING
Nie xinhua
ZHU JIAFANG
LING XIONGZHUANG

Assignees

深圳市汇尚科科技有限公司

Dates

Publication Date: 20260505
Application Date: 20260228

Claims (10)

1. An anti-hair loss essence containing plant source exosomes is characterized by comprising the following components in percentage by mass: 5-10% of plant source exosomes; 3-5% of a hair growth nutrient; 1-3% of a hair follicle blood circulation activating agent; 2-6% of a penetration enhancer; 1.5-2% of an oil-control bacteriostat; 0.45-1% of preservative; 77.5-82.75% of solvent; the plant exosome is formed by mixing a biota orientalis exosome solution, a polygonum multiflorum root exosome solution, a black ginseng root exosome solution and a murraya paniculata exosome solution according to a mass ratio of 3-5:1-1.5:1-1.5:4-6.
2. The hair loss preventing essence containing plant source exosomes according to claim 1, wherein the hair growth promoting nutrient is one or more of vitamins and polypeptides.
3. The hair loss preventing essence containing plant source exosomes according to claim 2, wherein the vitamin is one or more of D-panthenol, pyridoxine hydrochloride, tocopherol, nicotinamide, pantothenic acid, and the polypeptide is one or more of blue copper peptide, acetyl tetrapeptide-3, acetyl tetrapeptide-5, palmitoyl tripeptide-5, biotin tripeptide-1, and dipeptide-1.
4. The hair follicle blood circulation activating agent containing plant source exosomes of claim 1, wherein the hair follicle blood circulation activating agent is one or more of caffeine, ginger essential oil, borneol.
5. The anti-hair loss essence containing plant source exosomes according to claim 1, wherein the penetration enhancer is one or more of dimethyl sulfone, propylene glycol, menthol, butyl essential oil.
6. The anti-hair loss essence containing plant source exosomes according to claim 1, wherein the oil-controlling bacteriostat is one or more of zinc PCA, capryloglycine, salicylic acid, piroctone olamine.
7. The anti-hair loss essence containing plant source exosomes according to claim 1, wherein the preservative is one or more of phenoxyethanol, ethylhexyl glycerol, and clomazone.
8. The anti-hair loss essence containing plant-derived exosomes according to claim 1, wherein the solvent is one or more of ethanol and ginseng root water.
9. The method for preparing the anti-hair loss essence containing plant-derived exosomes according to any one of claims 1 to 8, comprising the following steps: A. adding a solvent into a batching pot, and slowly starting a stirrer, wherein the stirring frequency is 15-30 r/min; B. the hair follicle blood circulation activating agent, the permeation promoting agent and the oil control bacteriostat are respectively weighed and put into a batching pan, stirring until the mixture is completely dissolved, wherein the stirring frequency is 15-30 r/min; C. Weighing hair growth nutrition agent, putting into a batching pot, stirring until the hair growth nutrition agent is completely dissolved, then adding preservative, and stirring until the hair growth nutrition agent is uniformly mixed; D. adjusting the pH value, and controlling the pH value to be 5.0-6.5; E. weighing plant source exosomes, putting into a batching pot, and stirring at a low speed until the plant source exosomes are uniformly mixed, thus obtaining the anti-hair loss essence containing the plant source exosomes.
10. The method for preparing the anti-hair loss essence containing plant-derived exosomes according to claim 9, wherein the preparation of the plant-derived exosomes comprises the following steps: (1) Repeatedly scrubbing cacumen Platycladi or radix Polygoni Multiflori or radix Ginseng Rubra or herba Euphorbiae Humifusae with pure water, cutting, placing in a pulverizer, adding sterile PBS buffer solution of equal quality, pulverizing, squeezing juice, filtering the juice obtained by squeezing juice with sterile gauze to remove residue, and collecting filtrate; (2) Centrifuging the filtrate obtained in the step (1) at 1000 Xg for 10min, discarding larger sediment at the bottom of the centrifuge tube, removing residues, and collecting a first supernatant; (3) Centrifuging the first supernatant obtained in the step (2) at 3000 Xg for 20min, discarding the precipitate at the bottom of the centrifuge tube, removing residues and macromolecular protein impurities, and collecting a second supernatant; (4) Centrifuging the second supernatant obtained in the step (3) at 10000 Xg for 30min, discarding cell debris impurities at the bottom of the centrifuge tube, and collecting a third supernatant; (5) Centrifuging the third supernatant obtained in the step (4) at 5+/-1 ℃ for 50min at 30000 Xg, and collecting a fourth supernatant; (6) Centrifuging the fourth supernatant obtained in the step (5) at 5+/-1 ℃ and 50000 Xg for 50min, and collecting a fifth supernatant; (7) Centrifuging the fifth supernatant obtained in the step (6) at 5+/-1 ℃ and 80000 Xg for 60min, and collecting a sixth supernatant; (8) Centrifuging the sixth supernatant obtained in the step (7) at 5+/-1 ℃ and 100000 Xg for 90min, discarding the supernatant, adding a proper amount of sterile PBS buffer solution into the obtained precipitate for resuspension, and dissolving again to obtain a cacumen biotae exosome solution or a polygonum multiflorum root exosome solution or a black ginseng root exosome solution or a herba nigellae exosome solution.

Description

Hair loss preventing essence containing plant source exosomes and preparation method thereof Technical Field The invention belongs to the field of anti-hair loss cosmetics, and in particular relates to an anti-hair loss essence containing plant source exosomes and a preparation method thereof. Background With the continuous development of society, the pace of human life is accelerated, the working intensity is increased continuously, life work and rest and diet are irregular, and alopecia occurs to various degrees for many people. The statistics show that the male pattern alopecia rate in China reaches 20 percent. In recent years, the trend of alopecia has been reduced, the proportion of alopecia before age 30 is higher than 84%, and the alopecia team between ages 18 and 25 is increasingly large, so that the problem of alopecia is increasingly serious. Alopecia is a clinically common disorder, and is classified into temporary alopecia and permanent alopecia according to the clinical manifestations of patients suffering from alopecia. Temporary alopecia is a symptom of alopecia caused by disorder or disturbance of partial nerve metabolism of head skin, physiological function degradation or malnutrition of hair follicle, and permanent alopecia is generally senile alopecia or is caused by heredity, endocrine, certain diseases or medicines, mental and psychological effects, etc. At present, the recognized therapeutic drugs minoxidil and finasteride have therapeutic effects, but have larger drug dependence and side effects, the former can shorten the telogen period and prolong the growing period of hair follicle growth, promote the increase of the diameter and length of hair follicle, and further promote hair growth, but have higher compliance requirements, once stopped, the drug can often cause more serious alopecia and generate bad symptoms such as scalp itching, desquamation, contact dermatitis, depression and the like. The latter can prevent testosterone from being converted into dihydrotestosterone, so that injury to hair follicles is reduced, but adverse reactions such as sexual dysfunction are often caused during the administration of finasteride. Both drugs are easy to relapse after stopping taking, and also cause skin irritation, scalp itching, facial and hand hirsutism, dizziness, anorexia, tachycardia and the like. In addition, although hair transplantation has a certain effect, the cost is high due to the great surgical trauma, and the clinical application of the hair transplantation is limited due to the need of multiple surgeries. The effect of non-drug treatment such as acupuncture is also not ideal. Therefore, searching for more effective, low-toxicity, safe and economical therapeutic drugs becomes a major topic in the development of new drugs for treating alopecia. In recent years, more and more research reports on treating alopecia by using plant extracts such as traditional Chinese medicines and the like are provided, and the plant source components are relatively safe and nontoxic and are convenient to use. However, efficacy is not ideal due to unreasonable formulation or improper preparation process, and plant extracts may also have stability problems. Extracellular vesicles secreted by cells and having a diameter of 30-200nm are rich in bioactive substances such as nucleic acids, proteins, lipids and sugar complexes. However, exosomes involved in current research are mainly derived from mammals, and have low yield, high cost and complex separation process, and cannot be produced and applied on a large scale. Therefore, finding exosomes with wide sources, high yield and easy separation becomes a research hotspot for researchers. The plant source exosome is a natural nano vesicle separated from plant tissues, is secreted by living cells of plants, has the characteristics of low immunity, easiness in penetrating cell membranes and the like, can mediate communication among cells, can be used as a drug carrier for transporting therapeutic drug molecules to target spots, and is commonly used for effectively transporting small molecular chemical drugs and gene drugs. In addition, the cell culture medium also has the function of regulating the physiological functions of cells of other species, and is a hot spot for development and research in recent years. The Chinese arborvitae twig, the tuber fleeceflower root and the black ginseng root are one of the prescription drugs with highest frequency of use in the external prescription for treating alopecia in traditional Chinese medicine, and are recorded in ancient books of traditional Chinese medicines such as "urgent prescription for treating elbow", ben Cao gang mu ", zhonghua Ben Cao and Yuan nan Ben Cao". At present, a great deal of research is focused on the formulation of Chinese herbal medicine preparations, and research and application of plant source exosome vesicles in hair growth and hair loss prevention are not reported at present. The her