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CN-121971328-A - Withania somnifera fermentation method, fermentation product and application thereof

CN121971328ACN 121971328 ACN121971328 ACN 121971328ACN-121971328-A

Abstract

The invention belongs to the field of new daily chemical materials, and discloses a withania fermentation method, which comprises the step of fermenting withania root by one or more strains selected from lactobacillus, bifidobacterium, bacillus, micrococcus, saccharomycetes and filamentous fungi to obtain a withania fermentation product. The obtained withania fermentation product has anti-wrinkle and tightening effects in cosmetics, and is obviously superior to fermentation product obtained by fermenting withania extract as fermentation base. Meanwhile, the invention also provides application of the fermentation product.

Inventors

  • Luo Peiyin
  • GENG LIN
  • ZHANG WENYUN
  • GU SHAOJIE
  • LI FUYAN
  • LI DONGCUI
  • HE TINGGANG
  • HUANG YAN

Assignees

  • 广东省禾基生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260107

Claims (12)

  1. 1. A method for fermenting withania is characterized in that withania root is fermented by one or more strains selected from lactobacillus, bifidobacterium, bacillus, micrococcus, saccharomycetes and filamentous fungi to obtain a withania fermentation product.
  2. 2. The method of fermenting withania according to claim 1, characterized in that the lactic acid bacteria are one or more combinations of lactobacillus plantarum, lactobacillus sake subspecies lactobacillus paracasei; The bifidobacterium is one or a combination of a plurality of bifidobacterium longum subspecies infancy and bifidobacterium adolescentis longum; The bacillus is bacillus subtilis and/or bacillus aryabhattai; the micrococcus is a micrococcus strain in the genus Micrococcus and the genus Micrococcus; The saccharomycete is Saccharomyces cerevisiae and/or Saccharomyces cerevisiae; the filamentous fungus is Aspergillus niger and/or Aspergillus oryzae.
  3. 3. The method for fermenting withania according to claim 1, wherein the fermentation temperature and the fermentation oxygen content are set according to the fermentation requirement of the fermentation strain, the fermentation medium is a medium containing 1-20wt% of withania root powder, preferably the fermentation medium is a medium containing 5-15wt% of withania root powder, preferably the fermentation medium is a medium containing 7-13wt% of withania root powder.
  4. 4. The method for fermenting withania according to claim 1, wherein the medium contains only withania root powder and water, and no additional carbon source, nitrogen source and inorganic salt are added.
  5. 5. The method of fermenting withania according to claim 1, characterized in that the fermentation time is 10-48 hours, preferably 16-32 hours, preferably 20-28 hours.
  6. 6. The method for fermenting withania according to claim 1, characterized in that the fermentation is anaerobic fermentation if the fermentation bacteria are bifidobacteria, aerobic fermentation if the fermentation bacteria are bacillus and/or micrococcus, preferably the aerobic fermentation is aerobic fermentation in a shaker, and fermentation in an air atmosphere if the fermentation bacteria are lactic acid bacteria, saccharomycetes and filamentous fungi.
  7. 7. The withania fermentation method according to claim 1, characterized in that the fermentation temperature is 28-40 ℃, preferably 37±2 ℃, preferably 37±1 ℃, preferably 37±0.5 ℃ if the fermentation bacteria are lactic acid bacteria, bifidobacteria, bacillus; If the fermentation bacteria are micrococcus, yeast or filamentous fungi, the fermentation temperature is 30.+ -. 2 ℃, preferably 30.+ -. 1 ℃, and more preferably 30.+ -. 0.5 ℃.
  8. 8. The method of fermenting withania according to claim 1, characterized in that the inoculum size of the strain is 1-30 wt%, preferably 5-20 wt%, preferably 7-15 wt%.
  9. 9. A withania fermentation product obtainable by a process as claimed in any one of claims 1 to 8.
  10. 10. A withania extract characterized in that it is obtained by directed enrichment of withanolides with a polyol-mediated solubilization of the withanolides fermentation product according to claim 9.
  11. 11. Use of the withania fermentation product according to claim 9 for the preparation of a cosmetic product, which is a cosmetic product with one or more functions of anti-wrinkle, tightening.
  12. 12. A cosmetic is characterized by comprising 0.05% -50% of withania fermentation product.

Description

Withania somnifera fermentation method, fermentation product and application thereof Technical Field The invention relates to the field of new daily chemical materials, in particular to a withania fermentation method, a fermentation product and application thereof. Background The prior art discloses the related technology of withania fermentation as follows: Patent application with publication number ES2848828T3, subject matter of application of withania extract for the treatment of amyloid-related diseases; publication number US11129864B2, the subject is a process for obtaining plant extracts and related compositions; of particular note is patent application publication number CN112075629a, the subject of which is a method of enhancing the antioxidant activity of withania somnifera extract by probiotic fermentation, which discloses fermenting withania somnifera extract by probiotics to enhance antioxidant properties. The above schemes all use withania extract as the fermentation base, but after further investigation of the present application it was found that further improvement can be achieved in specific cosmetic efficacy. Disclosure of Invention The invention aims to provide a withania fermentation method, and the withania fermentation product obtained by fermentation by the method has obviously better functions of compacting cosmetics, resisting wrinkles and the like than a fermentation product which is not fermented and takes withania extract as a fermentation basis. Meanwhile, the invention also provides application of the fermentation product. In order to achieve the aim, the invention provides the following technical scheme that the withania fermentation method adopts one or more strains of lactobacillus, bifidobacterium, bacillus, micrococcus, saccharomycetes and filamentous fungi to ferment withania root, so as to obtain withania fermentation product. In the cigar fermentation method, the lactobacillus is one or a combination of more than one of lactobacillus plantarum, lactobacillus sake subspecies of lactobacillus plantarum and lactobacillus paracasei; The bifidobacterium is one or a combination of a plurality of bifidobacterium longum subspecies infancy and bifidobacterium adolescentis longum; The bacillus is bacillus subtilis and/or bacillus aryabhattai; the micrococcus is micrococcus luteus and/or one strain of micrococcus; The saccharomycete is grain wine saccharomycete and/or Saccharomyces trigonensis; the filamentous fungus is Aspergillus niger and/or Aspergillus oryzae. In the above-mentioned cigar fermentation method, the fermentation temperature and the fermentation oxygen content are set according to the fermentation requirement of the fermentation strain, the fermentation medium is a medium containing 1-20wt% of withania root powder, preferably the fermentation medium is a medium containing 5-15wt% of withania root powder, preferably the fermentation medium is a medium containing 7-13wt% of withania root powder. In the above-mentioned cigar fermentation method, the culture medium only contains withania root powder and water, and no additional carbon source, nitrogen source and inorganic salt are added. In the cigar fermentation method, the fermentation time is 10-48 hours, preferably 16-32 hours, and preferably 20-28 hours. In the above-mentioned cigar fermentation method, the fermentation is anaerobic fermentation if the fermentation bacteria are bifidobacteria, aerobic fermentation if the fermentation bacteria are bacillus and/or micrococcus, preferably, the aerobic fermentation is aerobic fermentation in a shaker, and fermentation in an air atmosphere if the fermentation bacteria are lactobacillus, saccharomycetes and filamentous fungi. In the above-mentioned withania fermentation method, the fermentation temperature is 28-40 ℃, preferably, if the fermentation bacteria are lactobacillus, bifidobacterium and bacillus, the fermentation temperature is 37±2 ℃, preferably 37±1 ℃, preferably 37±0.5 ℃; If the fermentation bacteria are micrococcus, yeast or filamentous fungi, the fermentation temperature is 30.+ -. 2 ℃, preferably 30.+ -. 1 ℃, and more preferably 30.+ -. 0.5 ℃. The inoculation amount of the strain is 1-30wt%, preferably 5-20wt%, and preferably 7-15wt%. In the invention, the live bacteria content of the strain is 10 6-108 cfu/ml. The invention also discloses a withania fermentation product obtained by adopting any one of the methods. In addition, a withania root extract is also disclosed, and the withania lactone is directionally enriched by adopting polyalcohol to mediate solubilization aiming at the withania fermentation product. The polyalcohol is one or more of propylene glycol, butanediol, pentanediol and hexanediol, the polyalcohol and the withania fermentation product are mixed according to the weight ratio of 1-50:50-99, and the mixture is fully mixed, extracted, centrifuged and filtered, and the supernatant is the withania root extract. In the application of prep