CN-121971355-A - Preparation method and application of enzymatic royal jelly capable of whitening skin, removing spots and resisting wrinkles

Abstract

The invention discloses a preparation method and application of enzymatic royal jelly capable of whitening, removing spots and resisting wrinkles, and belongs to the technical field of enzymolysis of royal jelly. The technical problem to be solved is that the existing royal jelly zymolyte lacks research on whitening, freckle-fading and wrinkle-resisting effects. The technical scheme is that the preparation method of the enzymolysis royal jelly comprises the following steps of (1) mixing the royal jelly with water, homogenizing, regulating pH to obtain a royal jelly solution, (2) adding compound protease into the royal jelly solution for enzymolysis, inactivating enzyme, and carrying out solid-liquid separation to obtain the enzymolysis liquid of the royal jelly, wherein the compound protease in the step (2) is ficin, chymotrypsin and flavourzyme.

Inventors

• WANG WANG
• WANG LING
• YU JIANHUI
• YU CHUNTAO
• LI HONGTAO
• AI DAN

Assignees

• 江西汪氏蜜蜂园有限公司

Dates

Publication Date

20260505

Application Date

20260408

Claims (10)

1. 1. The preparation method of the enzymatic hydrolysis royal jelly for whitening, removing spots and resisting wrinkles is characterized by comprising the following steps of: (1) Mixing Lac Regis Apis with water, homogenizing, and regulating pH to obtain Lac Regis Apis solution; (2) Adding compound protease into the royal jelly solution for enzymolysis, inactivating enzyme, and performing solid-liquid separation to obtain the royal jelly enzymolysis solution; The compound protease in the step (2) is ficin, chymotrypsin and flavourzyme.
2. 2. The method for preparing enzymatic hydrolysate of royal jelly according to claim 1, characterized in that the mass ratio of ficin, chymotrypsin and flavourzyme is 1:1-3:1-5.
3. 3. The method for preparing enzymatic hydrolysate of royal jelly according to claim 2, characterized in that the mass ratio of ficin, chymotrypsin and flavourzyme is 1:1-3:3-5.
4. 4. The method for preparing enzymatic hydrolysate of royal jelly according to claim 2, characterized in that the mass ratio of ficin, chymotrypsin and flavourzyme is 1:2:4.
5. 5. The method for preparing enzymatic hydrolysis royal jelly according to claim 1, characterized in that the mass ratio of the royal jelly to water in step (1) is 1:10-15; The pH value is adjusted in the step (1) to 7-8; homogenizing under 5-15MPa for at least 1 time and 3-5min each time in the step (1).
6. 6. The method of claim 5, wherein the homogenization in step (1) is performed for 2 to 3 times at 8 to 12MPa for 3 to 5 minutes each time.
7. 7. The method for preparing enzymatic hydrolyzed royal jelly according to claim 1, wherein the amount of the complex protease added in the step (2) is 0.3% -1.2% of the royal jelly solution.
8. 8. The method of claim 7, wherein the amount of the complex protease added in the step (2) is 0.5% -1% of the royal jelly solution.
9. 9. The method for preparing enzymatic royal jelly according to claim 1, wherein the enzymatic hydrolysis in step (2) is carried out at 45-55 ℃ for 12-24 hours.
10. 10. The use of the enzymatic royal jelly prepared by the method of any one of claims 1 to 9 in the preparation of foods, health products, medicines or cosmetics.

Description

Preparation method and application of enzymatic royal jelly capable of whitening skin, removing spots and resisting wrinkles Technical Field The invention belongs to the technical field of enzymolysis of royal jelly, and particularly relates to a preparation method and application of enzymolysis royal jelly capable of whitening, removing spots and resisting wrinkles. Background Royal Jelly (Royal Jelly) is milk white or light yellow slurry secreted by glands such as developed hypopharynx gland and palate gland of worker bee, has biological activities such as immunoregulation, antibacterial, antioxidant and antiaging, and is widely used in fields such as health product and cosmetics. Although the royal jelly protein has remarkable biological activity, the royal jelly protein has the defects of large molecular weight, difficult absorption and utilization by human skin and the like. Therefore, the increase of the absorption rate and the efficacy of the royal jelly protein has important significance for the development of products thereof. The proteolysis technology can decompose protein into small-molecule peptide fragments, has the characteristics of high safety, high biological activity, easy absorption, high target specificity and the like compared with original protein, and can effectively improve the biological activity and the added value of the royal jelly protein. Patent document CN119570889a (publication date 2025, 03, 07) discloses a royal jelly protein hydrolysate with melanin inhibiting activity, which is obtained by subjecting a soluble royal jelly protein solution to pepsin enzymolysis to obtain an enzymolysis solution, and subjecting the enzymolysis solution to ultrafiltration separation to obtain a polypeptide solution with a molecular weight of less than 3KDa, namely the royal jelly protein hydrolysate. The royal jelly protein hydrolysate has good melanin generation inhibiting activity and can reduce the generation of mouse melanoma cells and zebra fish melanin, but the invention does not pay attention to the efficacy of the royal jelly protein hydrolysate in resisting wrinkles. The patent document CN118649101A (publication date: 09 month 17 of 2024) discloses a royal jelly protein peptide powder, which is prepared by mixing royal jelly and a solvent in a mass ratio of 1 (10-20), filtering by using a microfiltration membrane, collecting a trapped fluid, detecting the pH of the trapped fluid, stopping filtering when the pH reaches 4.0-4.3, performing enzymolysis extraction, namely concentrating the trapped fluid to a protein concentration of 1% -2%, regulating the pH to 6.5-7.5, adding a complex enzyme for enzymolysis, boiling, inactivating, and obtaining a proteolytic liquid, performing separation and purification, namely filtering the proteolytic liquid, separating, obtaining filtrate, concentrating and drying, namely concentrating the filtrate to a solid content of 3% -5%, and performing freeze drying, thus obtaining the royal jelly protein peptide powder. The royal jelly protein peptide powder has effects of inhibiting excessive free radicals, inhibiting matrix metalloproteinase, inhibiting elastase activity, and promoting collagen secretion. The invention provides the royal jelly protein peptide powder prepared by enzymolysis of the royal jelly, which has the effects of resisting wrinkle and preventing aging, but does not pay attention to the effects of whitening and fading spots. In summary, the enzymolysis technology can effectively improve the stability and physiological effects of the royal jelly, but the prior art still has the problem of single effect of enzymolysis products, especially the study of whitening and fading spots is deficient, and the study of whitening and fading spots and anti-wrinkle effects is not found, so that development of the enzymolysis technology of the royal jelly with the whitening and fading spots and anti-wrinkle effects is needed urgently, and the enzymolysis technology has important significance in expanding the application field of the royal jelly and improving the added value of the products. Disclosure of Invention In order to solve the technical problems, the invention provides a preparation method and application of enzymolysis royal jelly for whitening, removing spots and resisting wrinkles. The enzymolysis royal jelly preparation method adopts compound protease for directional enzymolysis, and the prepared enzymolysis royal jelly has the double effects of whitening skin, reducing speckle and resisting wrinkle, can be widely used as raw materials in foods, health products, medicines and cosmetics, and greatly improves the product value of the royal jelly. Description of the terminology: unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. All patents, patent inventions, and publications cited herein are incorporated by reference in their entirety u