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CN-121971359-A - Composition containing ginseng exosomes and superelastin and application thereof

CN121971359ACN 121971359 ACN121971359 ACN 121971359ACN-121971359-A

Abstract

The invention discloses a composition containing ginseng exosomes and superelastin and application thereof, belonging to the technical field of biology. The ginseng exosome and superelastin-containing composition is prepared by compounding ginseng exosome and superelastin according to the weight ratio of 10 (100-150), wherein the ginseng exosome is prepared by extracting ginseng exosome from ginseng root tissues by utilizing a differential centrifugation method to obtain ginseng exosome crude extract, adding sucrose solutions with different concentrations into the ginseng exosome crude extract, and carrying out ultra-high speed centrifugation and purification to obtain the purified ginseng exosome, and the amino acid sequence of the superelastin is shown as SEQ ID NO. 3. The ginseng exosome and the superelastin are combined for the first time, wherein the ginseng exosome and the superelastin both have the effect of promoting cell proliferation, the use safety is high, and the ginseng exosome and the superelastin can synergistically improve the skin tightening and anti-wrinkle effects and the skin injury repairing effects.

Inventors

  • SHAN BAOLONG
  • LI RUXUE
  • HU ZHURAN
  • WANG XIANGYAN
  • LIAN XINHUI
  • YAN XIAONA
  • ZHANG AIZHEN
  • LIN XIANHUA
  • Shuai Yingxue
  • WANG HONG

Assignees

  • 山东凤凰生物科技股份有限公司

Dates

Publication Date
20260505
Application Date
20260211

Claims (10)

  1. 1. A composition containing ginseng exosomes and superelastin is characterized by being prepared by compounding ginseng exosomes and superelastin according to a weight ratio of 10 (100-150).
  2. 2. The composition of claim 1, wherein the ginseng exosomes are prepared by the following method: extracting Ginseng radix exosome from Ginseng radix tissue by differential centrifugation to obtain Ginseng radix exosome crude extract, adding sucrose solutions with different concentrations, and ultra-high speed centrifuging and purifying to obtain purified Ginseng radix exosome.
  3. 3. The composition according to claim 2, wherein the condition of differential centrifugation is that ginseng root tissue is homogenized, the homogenized fluid is centrifuged at 300 Xg for 10min to remove massive tissue fragments, the supernatant is centrifuged at 2000 Xg for 20min to remove cell fragments, and then centrifuged at 10000 Xg for 30min to remove organelles, and finally, the supernatant is centrifuged at 110000 Xg for 70min at ultra-high speed to discard the supernatant, and the precipitate is resuspended to obtain crude extract of ginseng exosomes.
  4. 4. The composition of claim 1, wherein the superelastin has an amino acid sequence as set forth in SEQ ID No. 3.
  5. 5. The composition of claim 1, wherein the composition is compounded from ginseng exosomes and superelastin in a weight ratio of 10:125.
  6. 6. Use of a composition according to any one of claims 1 to 5 in at least one of the following (1) - (4): (1) Preparing a product for promoting cell proliferation; (2) Preparing a product for inhibiting skin shrinkage; (3) Preparing a whitening product; (4) And preparing a product for promoting damage repair.
  7. 7. The use according to claim 6, wherein the product is in the form of a liquid formulation, a solid formulation, a semi-solid formulation, a lyophilized formulation, a dressing carrier or a culture medium additive.
  8. 8. A cosmetic comprising the composition containing ginseng exosomes and superelastin according to any one of claims 1 to 5 as an active ingredient.
  9. 9. The cosmetic according to claim 8, further comprising a lyoprotectant, wherein the lyoprotectant is prepared by compounding trehalose, mannitol and superelastin according to a weight ratio of 5:3:0.1, and the amino acid sequence of the superelastin is shown as SEQ ID No. 3.
  10. 10. The cosmetic product according to claim 8 or 9, wherein the cosmetic product is in the form of a lotion, a serum, an emulsion, a cream, a gel, a spray, a mask, an eye mask, a skin care oil, a cleansing product, a lip balm or a lyophilized powder formulation.

Description

Composition containing ginseng exosomes and superelastin and application thereof Technical Field The invention relates to the technical field of biology, in particular to a composition containing ginseng exosomes and superelastin and application thereof. Background Skin aging is a complex biological process that is primarily characterized by collagen and elastin degradation, disruption of extracellular matrix (ECM) structure, reduced skin barrier function, increased moisture loss, and visible signs of wrinkling, sagging, etc. Although the traditional anti-aging components such as retinol, vitamin C, peptides and the like have certain efficacy, the problems of poor stability, low transdermal absorption rate, strong irritation or single action mechanism and the like generally exist, and the requirements of consumers on efficient, safe and multi-target anti-aging products are difficult to meet. In recent years, plant-derived exosomes are receiving attention as emerging natural active carriers due to their unique nanoscale structure, good biocompatibility, excellent transmembrane delivery capacity and endogenous bioactivity. The ginseng exosomes are proved to be rich in microribonucleic acid (microRNA), polysaccharide, saponin and antioxidase, so that free radicals can be effectively removed, the activity of Matrix Metalloproteinases (MMPs) can be inhibited, fibroblast proliferation and collagen synthesis can be regulated and controlled, and the ginseng exosomes have remarkable anti-inflammatory, antioxidant and repair promoting functions. More importantly, the natural lipid bilayer membrane structure can be used as an intelligent carrier, so that the skin permeation efficiency and targeting of other active ingredients are improved. Elastin (ELastin) is a key structural protein that maintains skin elasticity and resiliency and is very susceptible to irreversible cross-linking and degradation during aging. However, natural elastin has a large molecular weight, is difficult to transdermally, and is easily inactivated. The superelastin is constructed by a self-assembly technology, has a nano-network structure of dynamic reversible non-covalent bonds (such as hydrogen bonds, hydrophobic effect and Van der Waals force), not only remarkably improves the stability and bioavailability of elastin fragments, but also forms a bionic elastic membrane on the skin surface, immediately improves skin feel and promotes endogenous elastic fiber regeneration for a long time. Although ginseng exosomes and superelastin each exhibit good skin care potential, in the prior art, exosomes are mostly used for drug delivery, while superelastin focuses on physical support, and there are few reports of synergistic integration of the two into the same product system. Disclosure of Invention The present invention is directed to a composition comprising ginseng exosomes and superelastin and its use against the above-mentioned prior art. In order to achieve the above purpose, the invention adopts the following technical scheme: In a first aspect of the present invention, there is provided a composition comprising ginseng exosomes and superelastin, wherein the composition is prepared by compounding ginseng exosomes and superelastin in a weight ratio of 10 (100-150). Preferably, the ginseng exosome is prepared by the following method: extracting Ginseng radix exosome from Ginseng radix tissue by differential centrifugation to obtain Ginseng radix exosome crude extract, adding sucrose solutions with different concentrations, and ultra-high speed centrifuging and purifying to obtain purified Ginseng radix exosome. Further, the differential centrifugation method is carried out under the conditions that the ginseng root tissue is homogenized, the homogenized fluid is centrifuged for 10min at 300 Xg to remove massive tissue fragments, the supernatant is centrifuged for 20min at 2000 Xg to remove cell fragments, then the supernatant is centrifuged for 30min at 10000 Xg to remove organelles, finally, the supernatant is centrifuged for 70min at 110000 Xg at ultrahigh speed, the supernatant is discarded, and the sediment is resuspended to obtain the ginseng exosome crude extract. Preferably, the amino acid sequence of the superelastin is shown as SEQ ID NO. 3. In some preferred embodiments of the invention, the composition is compounded from ginseng exosomes and superelastin in a weight ratio of 10:125. In a second aspect of the present invention there is provided the use of the above composition in at least one of the following (1) - (4): (1) Preparing a product for promoting cell proliferation; (2) Preparing a product for inhibiting skin shrinkage; (3) Preparing a whitening product; (4) And preparing a product for promoting damage repair. The composition containing ginseng exosome and superelastin has certain promotion effect on cell proliferation, high use safety, and also has the effects of tightening, resisting wrinkle, whitening, repairing damage an