CN-121971360-A - Lactic acid bacteria fermentation liquor based on glutamic acid chelated soluble pearl powder and preparation method thereof

Abstract

The invention discloses a lactobacillus fermentation broth based on glutamic acid chelated soluble pearl powder, which is obtained by taking pearl powder dissolved by glutamic acid as a fermentation substrate, and adding lactobacillus under the condition of pH of 4-4.7 for fermentation. The invention takes the pearl powder dissolved by glutamic acid as a fermentation substrate, takes lactobacillus as a strain for fermentation, and the obtained fermentation liquor is rich in antioxidant polypeptide, rare amino acid gamma-aminobutyric acid, short chain fatty acid butyric acid, lactic acid and other components beneficial to skin protection and skin whitening while retaining all the components of the pearl, and the glutamic acid is used as a carbon source for microbial fermentation and a nitrogen source for microbial fermentation in the fermentation process, so that the purity of the fermentation liquor is ensured, and the obtained fermentation liquor has high chemical stability and can be directly used as a raw material of cosmetics.

Inventors

• ZHOU SHUO
• LI MUYUAN
• WANG CHANGJIANG
• WU YONGJUN
• XU JIANCHUN
• WANG MINGLAI
• LI CAIGUANG

Assignees

• 海南京润珍珠生物技术股份有限公司

Dates

Publication Date

20260505

Application Date

20260226

Claims (5)

1. 1. The lactobacillus fermentation broth based on the glutamic acid chelated soluble pearl powder is characterized in that the fermentation broth is obtained by inoculating lactobacillus under the condition that the pH value is 4-4.7 by taking the pearl powder dissolved by glutamic acid as a fermentation substrate.
2. 2. The lactic acid bacteria fermentation broth based on glutamic acid chelate soluble pearl powder according to claim 1, wherein the mass ratio of the pearl powder to the glutamic acid to the lactic acid bacteria is (2-4): 5-15): 0.1-0.3.
3. 3. The method for preparing the lactic acid bacteria fermentation broth based on the glutamic acid chelate soluble pearl powder according to claim 1, comprising the following steps: Step S1, putting pearl powder into a fermentation tank, adding water, uniformly mixing, and heating to 85-95 ℃; step S2, adding glutamic acid into a fermentation tank, fully reacting, continuously adding the glutamic acid to adjust the pH to 4-4.7, and cooling to below 38 ℃ to obtain a solution to be fermented; And step S3, inoculating lactobacillus into the solution to be fermented, uniformly stirring, sealing, fermenting under the condition of pH of 4-4.7, and obtaining lactobacillus fermentation liquor based on glutamic acid chelate soluble pearl powder after solid-liquid separation and filtration.
4. 4. The method for preparing lactobacillus fermentation broth based on glutamic acid chelate soluble pearl powder according to claim 3, wherein in the step S3, the fermentation temperature is 32-38 ℃ and the fermentation time is 24-48 h.
5. 5. The lactic acid bacteria fermentation broth based on glutamic acid chelate soluble pearl powder according to claim 1, wherein the fermentation broth is directly used as a raw material for cosmetics.

Description

Lactic acid bacteria fermentation liquor based on glutamic acid chelated soluble pearl powder and preparation method thereof Technical Field The invention belongs to the technical field of cosmetics, relates to a lactobacillus fermentation broth for skin care and a preparation method thereof, and in particular relates to a fermentation broth prepared by taking pearl powder dissolved by glutamic acid as a substrate and fermenting the same by lactobacillus and a preparation method thereof. Background The pearl is formed by stimulating bivalve animals such as Pinctada martensii of Pteriidae, hyriopsis cumingii of Cristaceae, or Cristaria plicata. Is sphere-like, oblong, oval or bar-shaped, and has a diameter of 1.5-8 mm. The surface is white, light pink, light yellow green or light blue, semitransparent, smooth or slightly concave-convex. Has special color luster, hard quality, broken surface with layer patterns, light smell and light taste, and the prepared pearl powder is white. The "Chinese pharmacopoeia" 2025 edition records that pearls "are sweet, salty and cold in nature and taste and meridian tropism". It enters heart and liver meridians. [ Functions and indications ] tranquillize, calm the mind, remove nebula, detoxify, promote tissue regeneration, moisten skin and remove spots. Can be used for treating palpitation, insomnia, convulsion, epilepsy, conjunctival congestion, nebula, and skin macula. More than 90% of the components of the pearl are calcium carbonate, exist in aragonite crystals, and are rich in various human body beneficial elements such as magnesium, zinc, manganese, iron, cobalt and the like, and organic components such as twenty protein amino acids, various non-protein amino acids such as taurine and polysaccharide. Lactobacillus (Lactobacillus) is a generic term for various bacteria that can decompose sugars into lactic acid. Is widely distributed in nature, can grow in aerobic and anaerobic environments, has a protective effect on organisms, is used for fermenting beverages. Glutamic acid, an acidic amino acid with 2 carboxyl groups, is an organic compound with a chemical formula of C 5H9NO4, and has a chemical name of alpha-aminopentanedioic acid. Glutamic acid is colorless crystal, has delicate flavor, is slightly soluble in water, is soluble in hydrochloric acid solution, and has isoelectric point of 3.22. Glutamic acid is present in a large amount in cereal proteins, and the animal brain is also present in a large amount. Glutamate plays an important role in the protein metabolism in the organism and is involved in many important chemical reactions in animals, plants and microorganisms, and it is also the most abundant excitatory neurotransmitter in the vertebrate nervous system. Glutamic acid is an acidic amino acid and if applied directly to the skin, it may cause irritation to the skin. The pearl powder contains more than 90% of aragonite calcium carbonate crystals, and the pearl protein and aragonite crystals are alternately stacked and have compact structure, so that the nutrition components in the pearl powder are difficult to release, the pearl powder is difficult to be directly fermented by microorganisms, and the pearl powder must be dissolved. The traditional pearl microbial fermentation liquid is prepared by dissolving pearl powder with organic acid, and extracting insoluble substances, mainly pearl proteins and polysaccharides, from the pearl powder for fermentation. However, if the concentration of the unchelated calcium plasma is too high, the activity of the strain is inhibited and the fermentation is hindered. In the traditional fermentation liquid, various additives such as glucose and other reducing sugar substances are added as carbon sources, peptone or yeast extract is used as a nitrogen source, the additives are usually incompletely fermented, and the residual additives often become negative components of the cosmetics, so that the stability of the cosmetics is affected. Disclosure of Invention Therefore, the invention aims to provide the lactobacillus fermentation liquor based on the glutamic acid chelated soluble pearl powder and the preparation method thereof, wherein the pearl powder dissolved by the glutamic acid is used as a fermentation substrate, lactobacillus is used as a strain for fermentation, the obtained fermentation liquor is rich in antioxidant polypeptide, rare amino acid gamma-aminobutyric acid, short-chain fatty acid butyric acid, lactic acid and other components beneficial to skin protection and skin whitening while retaining all the components of the pearl, and the glutamic acid is used as a carbon source for microbial fermentation and a nitrogen source for microbial fermentation in the fermentation process, so that the purity of the fermentation liquor is ensured, and the obtained fermentation liquor has high stability and can be directly used as a raw material of cosmetics. The invention has the technical principle that lactobacillus is