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CN-121971361-A - Acne-removing essence containing probiotics metabolites and preparation method thereof

CN121971361ACN 121971361 ACN121971361 ACN 121971361ACN-121971361-A

Abstract

The invention relates to an acne-removing essence containing probiotics metabolites and a preparation method thereof, and belongs to the technical field of acne-removing essence; the acne-removing essence comprises lactobacillus reuteri metabolites, tea tree essential oil, nicotinamide, centella asiatica extract, ganoderma lucidum extract, buddleia macrophylla extract, microcapsule-coated salicylic acid, alpha-arbutin, panthenol, trehalose, sodium hyaluronate, dipotassium glycyrrhizinate, ethylhexyl glycerol, 1, 2-butanediol, 1, 2-pentanediol, PCA zinc, xanthan gum, levulinic acid and deionized water, has obvious antibacterial and anti-inflammatory effects, is mild and low-sensitive, is suitable for most people, can show excellent effects in experiments such as microecology, cell and safety, effectively inhibits acne, pathogenic bacteria, balances skin microecology, plays a good acne-removing role, and has high industrialized value.

Inventors

  • YANG XI

Assignees

  • 广州一微生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260226

Claims (10)

  1. 1. The acne-removing essence containing the probiotics metabolites is characterized by comprising lactobacillus reuteri metabolites, tea tree essential oil, nicotinamide, centella asiatica extract, ganoderma lucidum extract, buddleia macrophylla extract, microcapsule-coated salicylic acid, alpha-arbutin, panthenol, trehalose, sodium hyaluronate, dipotassium glycyrrhizinate, ethylhexyl glycerol, 1, 2-butanediol, 1, 2-pentanediol, PCA zinc, xanthan gum, levulinic acid and deionized water.
  2. 2. The acne-removing essence containing probiotics metabolites according to claim 1, wherein the acne-removing essence comprises 2.5-3 parts by weight of lactobacillus reuteri metabolites, 0.05-0.08 parts by weight of tea tree essential oil, 2 parts by weight of nicotinamide, 1 part by weight of centella asiatica extract, 0.5 parts by weight of red sesame extract, 0.2-0.5 part by weight of buddleia longifolia extract, 0.2-0.4 part by weight of microcapsule-coated salicylic acid, 0.3 part by weight of alpha-arbutin, 1.5-2 parts by weight of panthenol, 1.4-2.5 parts by weight of trehalose, 0.4 parts by weight of sodium hyaluronate, 0.1-0.3 parts by weight of dipotassium glycyrrhizinate, 0.5 parts by weight of ethylhexyl glycerol, 1-1.5 parts by weight of glycerol, 0.5-1 parts by weight of 1, 2-butanediol, 0.5 parts by weight of 1, 2-pentanediol, 1.5 parts by weight of zinc, 0.84 parts by weight of levulinic acid, and 0.88 parts by weight of deionized water.
  3. 3. The acne-removing essence containing probiotics metabolites according to claim 1, wherein the acne-removing essence comprises 3 parts by weight of lactobacillus reuteri metabolites, 0.08 parts by weight of tea tree essential oil, 2 parts by weight of nicotinamide, 1 part by weight of centella asiatica extract, 0.5 parts by weight of red ganoderma lucidum extract, 0.3 parts by weight of Zostera marina extract, 0.3 parts by weight of microcapsule-coated salicylic acid, 0.3 parts by weight of alpha-arbutin, 2 parts by weight of panthenol, 1.5 parts by weight of trehalose, 0.4 parts by weight of sodium hyaluronate, 0.2 parts by weight of dipotassium glycyrrhizinate, 0.5 parts by weight of ethylhexyl glycerol, 1.2 parts by weight of glycerol, 0.5 parts by weight of 1, 2-butanediol, 0.5 parts by weight of 1, 2-pentanediol, 0.5 parts by weight of PCA zinc, 0.3 parts by weight of xanthan gum, 0.8 parts by weight of levulinic acid and 84.12 parts by weight of deionized water.
  4. 4. The acne-removing essence containing the probiotics metabolite according to claim 1, characterized in that the lactobacillus reuteri metabolite is prepared by the following steps: step S1, thawing frozen strains, inoculating 1% v/v of the frozen strains into MRS broth, and performing anaerobic culture to obtain first-stage seeds; step S2, inoculating the primary seeds into MRS broth according to 5% v/v, and culturing to obtain secondary seeds; s3, adding an MRS culture medium into a fermentation tank, inoculating secondary seeds according to 5-10% v/v, and fermenting to obtain fermentation liquor; And S4, centrifuging the fermentation liquor, taking supernatant, carrying out rough filtration, taking filtrate, carrying out ultrafiltration and sterilization.
  5. 5. The acne-removing essence containing probiotics metabolites according to claim 4, wherein the strain of the frozen strain in the step S1 is Lactobacillus reuteri ATCC 55730, and the frozen medium of the frozen strain consists of 85wt% of MRS culture medium and 15wt% of glycerol.
  6. 6. The acne-removing essence containing the probiotics metabolite according to claim 4, wherein the anaerobic culture condition in the step S1 is that the temperature is 37 ℃, the stirring speed is 100-120rpm, and the time is 12-16 hours.
  7. 7. The anti-acne essence containing the probiotics metabolite according to claim 4, wherein the culturing condition in the step S2 is that the temperature is 37 ℃, the stirring speed is 100-200rpm, the time is 8-12h, the nitrogen scavenging time is 0.05-0.1vvm, and the pH is maintained at 5.8-6.2 by adding acid or alkali.
  8. 8. The acne-removing essence containing the probiotics metabolites according to claim 4, wherein the fermentation condition in the step S3 is that the temperature is 36-37 ℃, the stirring speed is 150-250rpm, the time is 18-24 hours, the glycerol is added until the time of 6-8 hours, the adding amount is 5-10g/L, the nitrogen scavenging is 0.05-0.2vvm, and the pH is maintained at 5.8-6 by adding acid or alkali in the period, and the tank pressure is 0-0.05MPa.
  9. 9. The acne removing essence containing the probiotics metabolites according to claim 4, wherein the centrifugation condition in the step S4 is that the temperature is 4-10 ℃, the centrifugal force is 6000 Xg, the time is 20-25min, the coarse filtration is that the water passes through filter membranes with the pore diameters of 5 microns, 1 micron and 0.45 micron in sequence, the ultrafiltration is that a PES ultrafiltration membrane with the molecular weight cutoff of 10kDa is adopted to concentrate to 3-5 times of volume concentration multiple under the conditions that the temperature is 20-25 ℃, the transmembrane pressure difference is 0.8-1.5bar and the linear velocity is 1-2m/S, and the sterilization is that the PES membrane with the pore diameter of 0.22 micron is adopted to filter and sterilize.
  10. 10. A method for preparing the acne-removing essence containing the probiotics metabolite according to any one of claims 1-9, which is characterized in that the preparation method comprises the following steps: Step A, stirring deionized water and xanthan gum for 20-25min at 25 ℃, adding glycerol, 1, 2-butanediol, 1, 2-pentanediol, trehalose, sodium hyaluronate and panthenol, stirring for 30-45min, mixing, adding nicotinamide, PCA zinc, dipotassium glycyrrhizinate and alpha-arbutin, stirring for 30-45min, mixing, adding centella asiatica extract, ganoderma lucidum extract and buddleja davidiana extract, and stirring for 10min to obtain a water phase mixture; Step B, at the temperature of 25 ℃, mixing tea tree essential oil, ethylhexyl glycerol and levulinic acid for 5-10min to obtain an oil phase mixture; and C, dropwise adding the oil phase mixture into the water phase mixture under the condition of 25-28 ℃, stirring for 10-15min after dropwise adding, adding the microcapsule-coated salicylic acid, stirring for 10min, stirring, dropwise adding the lactobacillus reuteri metabolite while stirring, stirring for 15min after dropwise adding, regulating the pH value to 5-6, prefiltering, and finally filtering.

Description

Acne-removing essence containing probiotics metabolites and preparation method thereof Technical Field The invention belongs to the technical field of acne-removing essence, and particularly relates to an acne-removing essence containing probiotics metabolites and a preparation method thereof. Background Acne commonly called as acne is a high-incidence chronic skin disease, is frequently generated in teenagers and adult groups, not only damages the appearance of skin, but also causes psychological problems such as anxiety, spelt and the like, so that the acne removing product is always a core requirement product in the skin care market, the research and development of the existing acne removing product is mainly focused on three major directions of antibiosis, oil control and exfoliating, common active ingredients comprise salicylic acid, tea tree essential oil and the like, however, the traditional ingredients have obvious defects when the acne removing effect is exerted, on one hand, the chemical exfoliating ingredients such as salicylic acid are strong in irritation, and the skin barrier is easily damaged after long-term use, so that the skin is dry and reddish, on the other hand, in order to make up the efficacy of a single ingredient, a plurality of ingredient compounding modes are adopted for realizing the synergistic effect, but the compatibility and compatibility safety among different ingredients are controversial. In recent years, along with the proposal and intensive research of skin microecological theory, people gradually recognize that skin flora balance plays an important role in maintaining skin health, and research shows that skin microecological unbalance is one of key causes of acne formation and recurrent attacks, and under the background, probiotics and metabolites thereof are used as novel active ingredients, and have multiple effects of regulating skin flora, improving inflammatory reaction and the like, so that the skin microecological balance is gradually a new direction for developing acne-removing products, however, the application of the skin microecological balance in the acne-removing products is still in an initial stage, and the problems of insufficient research depth and insignificant synergistic effect with other functional ingredients exist. Disclosure of Invention The invention aims to overcome the defects of the prior art and provide an acne-removing essence containing probiotics metabolites and a preparation method thereof. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the invention provides acne-removing essence containing probiotics metabolites, which comprises lactobacillus reuteri metabolites, tea tree essential oil, nicotinamide, centella asiatica extract, ganoderma lucidum extract, buddleia macrophylla extract, microcapsule-coated salicylic acid, alpha-arbutin, panthenol, trehalose, sodium hyaluronate, dipotassium glycyrrhizinate, ethylhexyl glycerol, 1, 2-butanediol, 1, 2-pentanediol, zinc PCA, xanthan gum, levulinic acid and deionized water. Preferably, the acne-removing essence comprises 2.5-3 parts by weight of lactobacillus reuteri metabolite, 0.05-0.08 part by weight of tea tree essential oil, 2 parts by weight of nicotinamide, 1 part by weight of centella asiatica extract, 0.5 part by weight of red ganoderma lucidum extract, 0.2-0.5 part by weight of buddleia macrophylla extract, 0.2-0.4 part by weight of microcapsule-coated salicylic acid, 0.3 part by weight of alpha-arbutin, 1.5-2 parts by weight of panthenol, 1.4-2.5 parts by weight of trehalose, 0.4 part by weight of sodium hyaluronate, 0.1-0.3 part by weight of dipotassium glycyrrhizinate, 0.5 part by weight of ethylhexyl glycerol, 1-1.5 part by weight of glycerol, 0.5-1 part by weight of 1, 2-butanediol, 0.5 part by weight of 1, 2-pentanediol, 0.5 part by weight of zinc, 0.3 part by weight of xanthane gum, 0.8-88 parts by weight of levulinic acid and 84 parts by weight of deionized water. Preferably, the acne-removing essence comprises 3 parts by weight of lactobacillus reuteri metabolite, 0.08 part by weight of tea tree essential oil, 2 parts by weight of nicotinamide, 1 part by weight of centella asiatica extract, 0.5 part by weight of ganoderma lucidum extract, 0.3 part by weight of buddleia longifolia extract, 0.3 part by weight of microcapsule-coated salicylic acid, 0.3 part by weight of alpha-arbutin, 2 parts by weight of panthenol, 1.5 parts by weight of trehalose, 0.4 part by weight of sodium hyaluronate, 0.2 part by weight of dipotassium glycyrrhizinate, 0.5 part by weight of ethylhexyl glycerol, 1.2 parts by weight of glycerol, 0.5 part by weight of 1, 2-butanediol, 0.5 part by weight of 1, 2-pentanediol, 0.5 part by weight of zinc, 0.3 part by weight of xanthan gum, 0.8 part by weight of levulinic acid and 84.12 parts by weight of deionized water. Preferably, the lactobacillus reuteri metabolite is prepared by the steps of: step S1, thaw