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CN-121971367-A - PCL fiber catheter and preparation method and application thereof

CN121971367ACN 121971367 ACN121971367 ACN 121971367ACN-121971367-A

Abstract

The invention relates to the technical fields of biomedical engineering and diabetes treatment, in particular to a PCL fiber catheter and a preparation method and application thereof. And winding the PCL on a receiving rod by adopting a melt spinning technology to obtain the PCL fiber catheter, wherein the number of winding layers is 8-32. The PCL fiber catheter provided by the invention improves the survival and the field planting of the pancreatic islet and improves the long-term curative effect of subcutaneous pancreatic islet transplantation by promoting subcutaneous vascularization.

Inventors

  • WANG SHUSEN
  • YANG JIUXIA
  • ZHAN YIXIANG
  • WANG YINGBO

Assignees

  • 天津市第一中心医院

Dates

Publication Date
20260505
Application Date
20260206

Claims (10)

  1. 1. A preparation method of a PCL fiber catheter is characterized in that polycaprolactone is wound on a receiving rod by adopting a melt spinning technology to obtain the PCL fiber catheter; The number of layers of winding is 8-32.
  2. 2. The method according to claim 1, wherein the number of layers of the winding is 12 to 28.
  3. 3. The method of claim 1, wherein the number of layers wound is 14-24.
  4. 4. The method of claim 1, wherein the number of layers wound is 16.
  5. 5. The method of claim 1, wherein the PCL has a molecular weight of 80000Da and a water content of less than 0.1%.
  6. 6. The method according to claim 1, wherein the conditions of the melt spinning technology comprise a temperature of 170 ℃, a mechanical pressure of 3kPa, a PCL fiber after melting extruded through a 17G spinning needle, a winding speed of 150rpm, and a drawing device moving frequency of 100Hz.
  7. 7. A PCL fiber catheter prepared by the preparation method according to any one of claims 1-6, wherein the average spinning diameter of the PCL fiber catheter is 277-287 μm, the fiber angle is 42-55 degrees, and the inner diameter of the PCL fiber catheter is 3mm.
  8. 8. Use of the PCL fiber catheter of claim 7 for the preparation of a medicament for the treatment of type I diabetes after subcutaneous pre-vascularization.
  9. 9. The use according to claim 8, wherein the preparation method of the subcutaneous pre-vascularization catheter comprises the steps of filling a silicone tube with the same inner diameter as the PCL fiber catheter into the PCL fiber catheter and then embedding the silicone tube into the subcutaneous part; The subcutaneous pre-vascularized catheter improves islet transplantation efficacy.
  10. 10. A medicament for the treatment of diabetes, characterized in that islet cells are transplanted into the subcutaneous pre-vascularized catheter interior cavity in the use according to claim 7, resulting in the medicament.

Description

PCL fiber catheter and preparation method and application thereof Technical Field The invention relates to the technical fields of biomedical engineering and diabetes treatment, in particular to a PCL fiber catheter and a preparation method and application thereof. Background Type 1 diabetes (t 1 d) is a metabolic disorder caused by absolute deficiency of insulin due to autoimmune destruction of islet beta cells. Islet transplantation is one of the ideal protocols for clinical treatment of type 1 diabetes, but faces many challenges. On the one hand, a severe shortage of quality pancreatic donors and, on the other hand, traditional portal vein islet transplantation can trigger an immediate blood-mediated inflammatory response (IBMIR), resulting in 60% of islets being destroyed early after transplantation, severely affecting islet survival and colonization. The subcutaneous tissue is an ideal substitution site for islet transplantation, and has the advantages of large surface area, capability of transplanting a large amount of cells by utilizing a minimally invasive technology, no portal vein transplantation side effect, easiness in monitoring and biopsy, and the like. However, subcutaneous vascularization is less and is not suitable for direct implantation of cells, which limits the clinical transformation of subcutaneous islet transplantation in the treatment of diabetes. Disclosure of Invention In order to solve the problems, the invention provides a PCL fiber catheter and a preparation method and application thereof, and aims to design a high polymer fiber catheter synthesized by using a melt spinning technology, and through initiating early inflammatory reaction, subcutaneous vascularization is promoted, so that islet survival and field planting are improved, and the long-term curative effect of subcutaneous islet transplantation is improved. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a preparation method of a PCL fiber catheter, which comprises the steps of winding polycaprolactone on a receiving rod by adopting a melt spinning technology to obtain the PCL fiber catheter; The number of layers of winding is 8-32. Preferably, the number of layers of winding is 12-28. Preferably, the number of layers of winding is 14-24. Preferably, the number of layers of the winding is 16. Preferably, the molecular weight of the PCL is 80000Da, and the water content is lower than 0.1%. Preferably, the conditions of the melt spinning technology comprise 170 ℃ of temperature, 3kPa of machine pressure, extrusion of the melted PCL fibers through a 17G spinning needle, 150rpm of winding speed and 100Hz of moving frequency of a traction device. The invention also provides the PCL fiber catheter prepared by the preparation method, wherein the average spinning diameter of the PCL fiber catheter is 277-287 mu m, the fiber angle is 42-55 degrees, and the inner diameter of the PCL fiber catheter is 3mm. The invention also provides application of the PCL fiber catheter in preparing a medicament for treating diabetes after percutaneous pre-vascularization of the PCL fiber catheter. Preferably, the preparation method of the subcutaneous pre-vascularization catheter comprises the steps of filling a silica gel tube with the same inner diameter as the PCL fiber catheter into the PCL fiber catheter and then embedding the silica gel tube into a subcutaneous part; The subcutaneous pre-vascularized catheter improves islet transplantation efficacy. The invention also provides a medicine for treating type I diabetes, which is obtained by transplanting islet cells into the internal cavity of the subcutaneous pre-vascularization catheter in the application of the technical scheme. The invention has the beneficial effects that: 1. aiming at the problems of less subcutaneous vascularization and poor vascularization, the invention designs a high polymer fiber catheter prepared by using a melt spinning technology, promotes subcutaneous vascularization through subcutaneous pre-embedding, establishes a good vascular network and applies the method to subcutaneous islet transplantation. 2. The fibrous catheter can obviously promote granulation tissue to be regenerated after being buried subcutaneously. 3. The fibrous catheter can obviously promote the revascularization capacity of subcutaneous parts after being pre-buried subcutaneously. 4. Through subcutaneous pre-embedding of the fiber catheter, the long-term curative effect of subcutaneous islet transplantation is obviously improved, and the glucose tolerance of recipient animals is improved. The invention provides a new thought for the research of the cooperation of biological materials and subcutaneous islet transplantation, and can greatly promote the research progress in the field. The curative effect of subcutaneous islet transplantation is improved through pre-vascularization, a personalized transplantation sche