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CN-121971370-A - Artificial saliva and preparation method of artificial saliva hydrogel

CN121971370ACN 121971370 ACN121971370 ACN 121971370ACN-121971370-A

Abstract

The invention discloses artificial saliva and a preparation method of an artificial saliva hydrogel, which comprises the following raw materials, by mass, of ：KCl：0.5-1g、K 2 HPO 4 ：0.2-0.4g、NaCl：0.2-0.8g、KH 2 P O 4 ：0.2-0.4g、CaCl 2 ：10.04g-20.8g、Nacaboxymethylecellulose：1.5-3g、 parts of erythritol 60-120mg, 495-1000ml of natural coconut juice and 5-10ml of glabrous sarcandra herb extract. The glabrous sarcandra herb extract is selected to realize anti-inflammatory and anti-tumor treatment, so that the glabrous sarcandra herb extract has remarkable effect on xerostomia caused by radiotherapy, and is more portable and unique in taste through a gel bead preparation form. The gel beads are used as one of preparations for local administration in the oral cavity, and after blasting, the medicine can be targeted to salivary glands below the oral mucosa, so that a rapid medicine effect is realized. Local direct administration to the oral mucosa may provide rapid drug action, activating small salivary glands within the buccal mucosa while avoiding systemic drug exposure and off-target effects.

Inventors

  • WANG PAN

Assignees

  • 海南医学院

Dates

Publication Date
20260505
Application Date
20231124

Claims (6)

  1. 1. The artificial saliva is characterized by comprising the following raw materials in parts by weight: KCl：0.5-1g、K 2 HPO 4 ：0.2-0.4g、NaCl：0.2-0.8g、KH 2 P O 4 ：0.2-0.4g、CaCl 2 ：10.04g-20.8g、Nacaboxymethylecellulose：1.5-3g、 60-120mg of erythritol, 495-1000ml of natural coconut juice and 5-10ml of glabrous sarcandra herb extract.
  2. 2. The artificial saliva according to claim 1, comprising the following raw materials ：KCl：0.5g、K 2 HPO 4 ：0.2g、NaCl：0.4g、KH 2 PO 4 ：0.2g、CaCl 2 ：10.04g、Nacaboxymethylecellulose：1.5g、 parts by mass of erythritol 60mg, natural coconut juice 495ml and glabrous sarcandra herb extract 5ml.
  3. 3. The artificial saliva according to claim 1, wherein the artificial saliva comprises the following raw materials ：KCl：1.0g、K 2 HPO 4 ：0.4g、NaCl：0.8g、K H 2 P O 4 ：0.4g、Ca Cl：20.08g、Nacaboxymethylecellulose：3g、 parts by mass of erythritol 120mg, natural coconut water 1000mL and glabrous sarcandra herb extract 10mL.
  4. 4. A method for preparing artificial saliva hydrogel according to any one of claims 1-3, comprising the steps of: Step 1, proportionally taking KCl, K2HPO4, naCl, KH2PO4, caCl2 and 60mg of erythritol for later use; Step 2, taking natural coconut juice and glabrous sarcandra herb extract according to a proportion, and mixing to obtain a mixed solution; step 3, dissolving the composition obtained in the step 1 in a mixed solution, and stirring the mixed solution to fully dissolve the composition; Step 4, regulating the pH value to 5-7 by using 1mol/L sodium hydroxide solution, uniformly stirring, heating and boiling for 45min, stirring and cooling to below 40 ℃, filtering to obtain artificial saliva, and standing overnight; And 5, preparing hydrogel from the artificial saliva through oxidizing sodium alginate and carboxymethyl chitosan to obtain the artificial saliva hydrogel.
  5. 5. The method for preparing artificial saliva hydrogel according to claim 4, wherein in step 5, the preparation of the artificial saliva hydrogel comprises the steps of: s501, preparing carboxymethyl chitosan into 10%wt/vol CMC aqueous solution in double distilled water; s502, preparing oxidized sodium alginate into DASA solution with the concentration of 5-20%wt/vol in double distilled water; S503, mixing 10% CMC aqueous solution and DASA solution according to a ratio of 1:1, placing the mixture in a 37 ℃ oven to form hydrogel after fully mixing, and detecting the gel forming time, swelling property and injectability of the hydrogel; s504, freeze-drying the obtained hydrogel for 24 hours; S505, soaking the freeze-dried hydrogel in artificial saliva for 24 hours to obtain the finished hydrogel.
  6. 6. The method for preparing artificial saliva hydrogel according to claim 5, wherein the preparation of oxidized sodium alginate comprises the steps of: dissolving a certain amount of sodium alginate in deionized water to obtain a uniform sodium alginate solution; Adding 3 times equivalent of sodium periodate to the solution, and adjusting the pH to 4.5; Then stirring for 3 hours under the dark condition, and then adding 30 times equivalent of glycol solution; After stirring for 1 hour, the reaction mixed solution is placed in a 3500Da dialysis bag for dialysis for 48 hours; And obtaining white flocculent powder, namely oxidized sodium alginate, after freeze drying.

Description

Artificial saliva and preparation method of artificial saliva hydrogel Technical Field The invention relates to the technical field of artificial saliva, in particular to artificial saliva and a preparation method of an artificial saliva hydrogel. Background Artificial saliva is a liquid simulating natural saliva and is used for treating xerostomia, chewing and dysphagia and other diseases. In recent years, with the development of scientific technology, research on artificial saliva has also been advanced to some extent. At present, research on artificial saliva mainly focuses on the component research that the components of artificial saliva should be as close to natural saliva as possible, including water, electrolyte, mucus, enzymes, etc., and common preparation methods include membrane separation, spray drying, freeze drying, etc. These methods each have advantages and disadvantages. Through searching, the Chinese patent with the application number of CN101524363B discloses artificial saliva, which is used for solving the problems of short acting time, poor stability and poor taste of the artificial saliva. The Chinese patent of application number CN105147818B discloses an artificial saliva and a preparation method thereof, which are used for solving the problem that the oral bacteria overgrow and the oral cleaning nursing of clinically serious patients is difficult to inhibit the bacterial growth. However, the application of artificial saliva is wide, and for the daily health care use, the oral restoration use and the beauty field, the application of artificial saliva to the oral mucosa, the application of artificial saliva in the treatment of chewing and dysphagia, the application of artificial saliva in the oral restoration and the beauty field, the safety evaluation of artificial saliva and the like are required to face a wide range of different people, wherein the artificial saliva can be used as a medicine or a medical instrument, so that the research of the artificial saliva is a comprehensive problem related to a plurality of fields and technologies, how to combine and optimize the formulation of the artificial saliva to meet the plurality of conditions is required to be continuously explored and developed, and therefore, the artificial saliva of the hydrogel agent is required. Disclosure of Invention The invention aims to solve the defects in the prior art and provides artificial saliva and a preparation method of an artificial saliva hydrogel. In order to achieve the above purpose, the present invention adopts the following technical scheme: according to one aspect of the present invention, there is provided an artificial saliva. The artificial saliva comprises the following raw materials in parts by weight: KCl：0.5-1g、K2HPO4：0.2-0.4g、NaCl：0.2-0.8g、KH2P O 4：0.2-0.4g、CaCl2：10.04g-20.8g、Nacaboxymethylecellulose：1.5-3g、 60-120mg of erythritol, 495-1000ml of natural coconut juice and 5-10ml of glabrous sarcandra herb extract. Further comprises the following raw materials ：KCl：0.5g、K2HPO4：0.2g、NaCl：0.4g、KH2P O4：0.2g、CaCl2：10.04g、Nacaboxymethylecellulose：1.5g、 parts by mass of erythritol 60mg, natural coconut juice 495ml and glabrous sarcandra herb extract 5ml. Further, the preparation comprises the following raw materials ：KCl：1.0g、K2HPO4：0.4g、NaCl：0.8g、KH2PO4：0.4g、CaCl：20.08g、Nacaboxymethylecellulose：3g、 parts by mass of erythritol 120mg, natural coconut water 1000mL and glabrous sarcandra herb extract 10mL. According to another aspect of the present invention, a method of preparing an artificial saliva hydrogel is provided. A method for preparing artificial saliva hydrogel, comprising the following steps: Step 1, proportionally taking KCl, K2HPO4, naCl, KH2PO4, caCl2 and 60mg of erythritol for later use; Step 2, taking natural coconut juice and glabrous sarcandra herb extract according to a proportion, and mixing to obtain a mixed solution; step 3, dissolving the composition obtained in the step 1 in a mixed solution, and stirring the mixed solution to fully dissolve the composition; Step 4, regulating the pH value to 5-7 by using 1mol/L sodium hydroxide solution, uniformly stirring, heating and boiling for 45min, stirring and cooling to below 40 ℃, filtering to obtain artificial saliva, and standing overnight; And 5, preparing hydrogel from the artificial saliva through oxidizing sodium alginate and carboxymethyl chitosan to obtain the artificial saliva hydrogel. Further, in step 5, the preparation of the artificial saliva hydrogel comprises the following steps: s501, preparing carboxymethyl chitosan into 10%wt/vol CMC aqueous solution in double distilled water; s502, preparing oxidized sodium alginate into DASA solution with the concentration of 5-20%wt/vol in double distilled water; s503, mixing 10% CMC aqueous solution and DASA solution according to a ratio of 1:1, placing the mixture in a 37 ℃ oven to form hydrogel after fully mixing, and detecting the gel forming time, swelling property and