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CN-121971432-A - Application of light-driven organic semiconductor nanomotor in preparation of products for promoting differentiation of neural stem cells

CN121971432ACN 121971432 ACN121971432 ACN 121971432ACN-121971432-A

Abstract

The application relates to the technical field of biomedicine, and discloses application of a light-driven organic semiconductor nanomotor in preparation of products for promoting differentiation of neural stem cells, wherein the organic semiconductor nanomotor is obtained by surface treatment of poly (3-hexylthiophene) with sodium dodecyl sulfate and n-propanol. According to the scheme, in-vivo experiments show that the P3HT nanometer motor injected in situ in the subventricular zone (V-SVZ) of the mouse can keep stable light response performance under the irradiation of visible light, and can promote the directional differentiation of the neural stem cells to the neural lineage (but not the glial cells). In addition, the organic semiconductor nano motor has good cell compatibility and whole body biosafety, and has good application prospect in the field of nerve regeneration medicine.

Inventors

  • PENG FEI
  • Rong Jinghui

Assignees

  • 中山大学

Dates

Publication Date
20260505
Application Date
20260209

Claims (10)

  1. 1. Use of a photo-driven organic semiconductor nanomotor for the preparation of a product for promoting differentiation of neural stem cells, wherein the preparation raw material of the organic semiconductor nanomotor comprises poly 3-hexylthiophene.
  2. 2. The method according to claim 1, wherein the organic semiconductor nanomotor is obtained by surface treatment of poly (3-hexylthiophene) with sodium dodecyl sulfate and n-propanol.
  3. 3. The method of claim 2, wherein the method for preparing the organic semiconductor nanomotor comprises the steps of: dissolving the poly 3-hexylthiophene in an organic solvent to obtain a P3HT solution, adding a sodium dodecyl sulfate solution and n-propanol, mixing, heating for reaction, and purifying to obtain the product.
  4. 4. The method according to claim 3, wherein the organic solvent is selected from the group consisting of chloroform, toluene, methylene chloride, and tetrahydrofuran; and/or the concentration of the P3HT solution is 1-10 mg/mL; and/or the concentration of the sodium dodecyl sulfate solution is 。
  5. 5. The method of claim 4, wherein the volume ratio of the P3HT solution to the sodium dodecyl sulfate solution to n-propanol is 1:0.5-2:0.005-0.05.
  6. 6. The method according to claim 1 to 5, wherein the product comprises a drug or a device.
  7. 7. The method of claim 6, wherein the function of the product for promoting differentiation of neural stem cells comprises promoting differentiation of neural stem cells into neurons.
  8. 8. A pharmaceutical composition characterized in that an active ingredient comprises the photo-driven organic semiconductor nanomotor according to any one of claims 1 to 7; the efficacy of the pharmaceutical composition includes promoting differentiation of neural stem cells into neurons.
  9. 9. The pharmaceutical composition of claim 8, wherein: the pharmaceutical composition also comprises pharmaceutically acceptable auxiliary materials.
  10. 10. An apparatus for promoting differentiation of neural stem cells, comprising a photoexcitation element and the photo-driven organic semiconductor nanomotor according to any one of claims 1 to 7; The light excitation element is used for emitting light with the wavelength of 525+/-15 nm and the light intensity of 4-12 mW/cm < 2 >.

Description

Application of light-driven organic semiconductor nanomotor in preparation of products for promoting differentiation of neural stem cells Technical Field The application relates to the technical field of biomedicine, in particular to application of an organic semiconductor nanomotor driven by light in preparation of products for promoting differentiation of neural stem cells. Background The micro-nano motor (Micro nanomotors, MNMs) is an intelligent functional device with autonomous movement capability under the micro-nano scale, and is considered as a core technical platform for breaking through the limit of mass transmission and diffusion in a biological system and realizing accurate diagnosis and treatment of diseases and efficient micro-scale operation. In the last decade, metal and metal oxide based micro-nanomotors have become a research hotspot. The motor can realize self-driven motion by catalytically decomposing chemical fuel, and the unique motion performance and catalytic property of the motor have great application potential in various fields of environmental remediation, cancer treatment, biological sensing and the like. However, clinical conversion of such conventional metal and metal oxide-based micro-nanomotors remains limited by a number of key issues, with biosafety being one of the key issues limiting the clinical conversion of motors. On the one hand, the movement of such motors relies on the addition of exogenous chemical fuels, such as hydrogen peroxide (H 2O2) and the like. The externally added chemical fuel has the common problems of poor biocompatibility and biological safety hazard. On the other hand, the metal/metal oxide motor is easy to corrode and degrade in a complex biological culture microenvironment, and generated corrosion byproducts such as heavy metal ions and the like can be accumulated in organisms, so that a series of biotoxicity problems such as cell damage, inflammatory reaction and the like can be caused. This limitation greatly limits the safety and effectiveness of such motors for in vivo applications. In order to solve the problem of biocompatibility caused by the dependence of external traditional chemical fuel driving, the design and research of optical drive type micro-nano motors are rapidly developed in recent years. The optical signal is used as a driving force, and the micro-nano motor system realizes accurate control of motor movement through the optical signal. The regulation and control of the micro-nano motor movement mode, direction and speed are realized by adjusting the parameters such as the light intensity, wavelength, pulse periodicity and the like. The flexible regulation and control of the optical signals can realize the rapid switching of the start-stop-turn of particles on the single particle level, and greatly improve the accuracy and controllability of the operation under the micro-nano scale. Meanwhile, the light response wave band of the light driving system is continuously expanding to the visible light and near infrared light regions, and the capability of the near infrared light to efficiently penetrate through organism tissues provides a new research thought for solving the problem of organism tissue penetrability of light regulation signals. At present, the main light response driving mechanism for driving the micro-nano motor to autonomously move comprises photo-thermophoresis driving caused by thermal gradient generated by light irradiation, self-diffusion driving induced by a photo-catalytic reaction product and self-electrophoresis driving formed by uneven distribution of charge carriers generated by light excitation. The diversified light response driving mechanism endows the light driving micro-nano motor with great application potential in the field of biological application. However, most of the light-driven micro-nano motor systems developed at the present stage still depend on traditional inorganic photocatalytic materials such as TiO 2 and Pt, and the biocompatibility of the metal and metal oxide materials is still to be improved. In recent years, conjugated polymers (Conjugated Polymers, CP) have been widely used in the electronics field with their good biocompatibility, flexibility and functional diversity. Wherein the cell-material interface based on conjugated polymers is one of the research hotspots of CP materials in the field of bioelectronics. The neural stem cells (Neural STEM CELLS, NSCS) are adult stem cells with self-renewal capacity and multidirectional differentiation capacity, and can be differentiated into nerve cells such as neurons, astrocytes, oligodendrocytes and the like. The differentiated neurons have the functions of electric signal conduction, synaptic connection and information processing, hopefully reconstruct functional nerve loops, and are helpful for relieving the influence caused by neurodegenerative diseases. Whereas glial cells mainly exert an auxiliary supporting effect. Studies have show