CN-121971485-A - Nanometer enzyme for preventing salmonella pullorum infection, preparation method and application

Abstract

The invention discloses a nano-enzyme for preventing salmonella pullorum infection, which comprises kaempferol and cerium ions, wherein the kaempferol-cerium compound nano-enzyme CeKae NPs is formed by coordination, and has superoxide dismutase SOD-like activity and catalase CAT-like activity. The invention also discloses a preparation method and application of the nano-enzyme for preventing salmonella pullorum infection. The CeKae NPs nano-enzyme comprises kaempferol and cerium ions, has high-efficiency SOD and CAT nano-enzyme activities, can realize antioxidation self-cascade nano-enzyme reaction, effectively eliminates active oxygen, and improves pathological environment.

Inventors

• PENG HUI
• CHENG CHANG
• YANG DIQI
• FAN WEIQIN
• LU WENJIE

Assignees

• 海南大学

Dates

Publication Date

20260505

Application Date

20260114

Claims (8)

1. 1. The nano enzyme for preventing the salmonella pullorum infection is characterized by comprising kaempferol and cerium ions, wherein the kaempferol and cerium ions form a kaempferol-cerium composite nano enzyme CeKae NPs through coordination, and the kaempferol-cerium composite nano enzyme has superoxide dismutase SOD-like activity and catalase CAT-like activity.
2. 2. The nanoenzyme for preventing salmonella pullorum infection of claim 1, wherein the nanoenzyme is capable of catalyzing a self-cascade reaction of: (1) SOD-like activity, converting superoxide anion O 2 -to hydrogen peroxide H 2 O 2 ; (2) CAT-like activity, the decomposition of hydrogen peroxide H 2 O 2 into oxygen O 2 and water H 2 O.
3. 3. The method for preparing the nano-enzyme for preventing salmonella pullorum infection according to claim 2, wherein the method comprises the following steps: Step1, dissolving cerium nitrate hexahydrate Ce (NO 3 ) 3 .6H 2 O in absolute ethyl alcohol, fully stirring and dissolving, and adding ammonia water to adjust the pH value to be 9-10; step 2, adding kaempferol into the solution obtained in the step 1, stirring for coordination reaction, and centrifugally collecting precipitate; and step 3, washing the precipitate obtained in the step2 with absolute ethyl alcohol for 3 times, and performing vacuum freeze drying to obtain the kaempferol-cerium composite nano enzyme CeKae NPs.
4. 4. The preparation method according to claim 3, wherein cerium nitrate hexahydrate Ce (NO 3 ) 3 .6H 2 O to absolute ethanol ratio is 10-15 mg/1 ml) in step 1.
5. 5. The method according to claim 3, wherein the amount of kaempferol added in the step 2 is 1-3 mg.
6. 6. The preparation method according to claim 2, wherein the coordination reaction is carried out in step2 by stirring at 35-40 ℃ for 2-4 hours.
7. 7. Use of CeKae NPs nm enzyme according to any one of claims 1-6 in the manufacture of a medicament for the prevention of pullorum disease.
8. 8. A medicament for preventing pullorum disease, comprising CeKae NPs nm enzyme according to any one of claims 1-6.

Description

Nanometer enzyme for preventing salmonella pullorum infection, preparation method and application Technical Field The invention belongs to the technical field of nano biological medicine materials, and particularly relates to nano enzyme for preventing salmonella pullorum infection, and a preparation method and application of the nano enzyme. Background Pullorum is an acute sepsis in birds caused by salmonella pullorum, to which chickens of all species of each age group are susceptible, but the mortality rate of infection decreases with age, and many infected chickens develop a latent and persistent infection. The newly-incubated chicks are highly susceptible to the disease, often show symptoms such as mental depression, diarrhea dehydration, inappetence, dyspnea and the like, and have higher mortality. While young and adult chickens have no obvious clinical symptoms, egg yield, fertility and hatchability are significantly reduced. The disease not only affects the life health of birds, but also causes serious economic loss to the breeding industry. At present, the bacteria are not thoroughly cleared in China, the transmission of the salmonella pullorum is difficult to control in many provinces, and the salmonella pullorum presents high drug resistance in the domestic partial areas and carries various drug resistance genes, so that the difficulty of thoroughly clearing is increased. Although the pathogenic mechanism of salmonella pullorum is not clear, the salmonella pullorum is a key pathogenic factor for intestinal injury of birds, and the relationship between intestinal barrier function and intestinal flora is bidirectional dynamic interaction, so that the regulation of intestinal flora and the alleviation of intestinal injury are of great significance for preventing salmonella pullorum. The existing technology for preventing the salmonella pullorum infection mainly comprises feeding traditional Chinese medicine preparations or beneficial bacteria. The traditional Chinese medicine preparation has high cost, and the long-term large-scale use increases the burden of farms. But the beneficial bacteria have poor stability, are easy to inactivate, are greatly influenced by external conditions, have unstable effects and have potential risks. Disclosure of Invention It is a first object of the present invention to provide nanoezymes for preventing salmonella pullorum infection. Solves the problems of high cost and poor stability of the existing scheme for preventing the salmonella pullorum infection. The second object of the present invention is to provide a method for preparing the above nanoenzyme. It is a third object of the present invention to provide the use of the nanoenzymes described above. The first technical scheme adopted by the invention is that the nano-enzyme for preventing salmonella pullorum infection comprises kaempferol and cerium ions, and the kaempferol-cerium compound nano-enzyme CeKae NPs is formed by coordination of the kaempferol and cerium, and has superoxide dismutase SOD-like activity and catalase CAT-like activity. The first technical scheme adopted by the invention is characterized in that: further, the above nanoenzyme is capable of catalyzing the self-cascade reaction of: (1) SOD-like activity, converting superoxide anion O 2 -to hydrogen peroxide H 2O2; (2) CAT-like activity, the decomposition of hydrogen peroxide H 2O2 into oxygen O 2 and water H 2 O. The second technical scheme adopted by the invention is that the preparation method of the nano-enzyme for preventing the salmonella pullorum infection comprises the following steps: Step1, dissolving cerium nitrate hexahydrate Ce (NO 3)3.6H2 O in absolute ethyl alcohol, fully stirring and dissolving, and adding ammonia water to adjust the pH value to be 9-10; step 2, adding kaempferol into the solution obtained in the step 1, stirring for coordination reaction, and centrifugally collecting precipitate; and step 3, washing the precipitate obtained in the step2 with absolute ethyl alcohol for 3 times, and performing vacuum freeze drying to obtain the kaempferol-cerium composite nano enzyme CeKae NPs. The first technical scheme adopted by the invention is characterized in that: further, cerium nitrate hexahydrate Ce (NO 3)3.6H2 O to absolute ethanol ratio 10-15 mg:1 ml) in step 1. Further, the adding amount of kaempferol in the step 2 is 1-3 mg. Further, in the step 2, the mixture is stirred for 2 to 4 hours at the temperature of 35 to 40 ℃ to carry out coordination reaction. The third technical scheme adopted by the invention is the application of CeKae NPs nanometer enzyme in preparing medicaments for preventing pullorum disease. The fourth technical scheme adopted by the invention is that the drug for preventing pullorum disease contains CeKae NPs nanometer enzyme as above. The beneficial effects of the invention are as follows: The invention provides CeKae NPs nanometer enzyme, which realizes the prevention of salmonella pullorum