CN-121971493-A - Application of cheese bacillus paracasei in preparation of products for improving male reproductive health

Abstract

The invention discloses application of cheese bacillus paracasei in preparation of a product for improving male reproductive health, and belongs to the technical field of microorganisms. Cheese-forming bacillus paracasei (Lacticaseibacillus paracasei) NHNK-633 was deposited with China Center for Type Culture Collection (CCTCC) at a accession number of M20251542, 7 of 2025. NHNK-633 has effects of producing L-citrulline, repairing aging vascular endothelial cells, promoting vasodilation, repairing aging prostate cells, and reducing prostatitis, and can be used for preparing products for improving male reproductive health.

Inventors

• SU DONGHAI
• LIU GUOQIANG
• Ning changchun
• FENG HAIXIA
• CHEN YIXING
• WU SONGTAO
• WANG XIAOQIAN
• ZHAO LU
• CUI QINGYU
• DONG RUONAN
• XIE LULU
• LI YUYING
• TAN LEILEI

Assignees

• 青岛诺和诺康生物科技有限公司

Dates

Publication Date

20260505

Application Date

20260313

Claims (10)

1. 1. Use of lactobacillus paracasei (Lacticaseibacillus paracasei) for the preparation of a product for improving reproductive health in men, characterized in that said lactobacillus paracasei is lactobacillus paracasei (Lacticaseibacillus paracasei) NHNK-633, which has been deposited in the China center for type culture collection, with a deposit number cctccc No. M20251542, on month 07 of 2025.
2. 2. The use of claim 1, wherein the improvement in male reproductive health comprises the production of L-citrulline, modulation of vascular endothelial cells, and improvement in prostatitis.
3. 3. The use of claim 2, wherein said modulating vascular endothelial cells comprises increasing survival of oxidatively damaged vascular endothelial cells ea.hy926.
4. 4. The use of claim 2, wherein said modulating vascular endothelial cells further comprises upregulating expression of a first related gene of oxidatively damaged vascular endothelial cells ea.hy926.
5. 5. The use according to claim 4 wherein the first related genes of vascular endothelial cells ea.hy926 comprise vascular endothelial growth factor gene VEGF, cyclooxygenase-2 gene COX2 and nitric oxide synthase gene iNOS.
6. 6. The use according to claim 2, wherein said improving prostatitis comprises increasing the survival rate of lipopolysaccharide LPS induced damaged prostate cells PC-3.
7. 7. The use of claim 2, wherein said ameliorating prostatitis further comprises down-regulating expression of a second associated gene of lipopolysaccharide LPS-induced damaged prostate cell PC-3 inflammation.
8. 8. The use according to claim 7, wherein the second genes associated with PC-3 inflammation of prostate cells comprise interleukin-6 gene IL-6, interleukin-8 gene IL-8 and interleukin-1 β gene IL-1 β.
9. 9. The use according to claim 1, wherein the Lactobacillus paracasei is a gram positive colony, is in the shape of a rod under a microscope, grows on an MRS flat plate, can form a white, smooth and round opaque circular colony with a clean and round surface, has a clean edge, can uniformly grow in a turbid manner in an MRS liquid culture medium, and has a white precipitate after a long time.
10. 10. Use according to claim 1, characterized in that the product is prepared from a preparation which is a viable, fermentation/secretion product of the cheese bacillus paracasei.

Description

Application of cheese bacillus paracasei in preparation of products for improving male reproductive health Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to application of lactobacillus paracasei in preparation of products for improving male reproductive health. Background Erectile Dysfunction (ED) refers to the inability of the penis to achieve and maintain a sufficient state of erection, the most common sexual dysfunction in men. With age, the incidence of ED increases, severely affecting quality of life. Physiological penile erection is a vascular response under neuromodulation. The sexual stimulation nerve signals are transmitted down from the hypothalamic erection center to the cavernous nerve, and then transmitted to the penile tissue through the non-adrenal non-cholinergic nerve, causing the nerve endings and endothelial cells to release bioactive factors, causing the cavernous congestion to distend. Since penile erection is essentially a neurovascular process, vascular endothelial dysfunction is one of the important causes of ED. In recent years, research on the pathogenesis of ED has focused mainly on the destruction of endothelial cell function by oxidative damage. Active oxygen not only reacts directly with NO to reduce it, but also reduces nitric oxide synthase activity, thereby affecting the relaxation of the cavernous body, resulting in ED. A plurality of factors such as endothelin-1, angiotensin and the like can activate NADPH oxidase, catalyze the generation of a large amount of active oxygen, and finally damage the penile erection function. Thus, protecting endothelial function has become a new approach to treating ED. Chronic Prostatitis (CP) is a common and frequently occurring disease of the urology department. Investigation showed that 43% of the patients with prostatitis suffer from erectile dysfunction. Inflammation of the prostate site will further exacerbate the ED symptoms, causing a vicious circle. The common preparations for treating ED at present mainly comprise antioxidants, folic acid, vitamins, polyphenols, carnitine, vasodilators, statin drugs and the like, but certain drugs have side effects such as headache and blood pressure rise, and the like, so that safe and effective treatment methods are needed. Disclosure of Invention In view of the above, it is an object of the present invention to provide the use of lactobacillus paracasei for the preparation of a product for improving reproductive health in men. The invention provides application of lactobacillus paracasei (Lacticaseibacillus paracasei) in preparing a product for improving male reproductive health, wherein the lactobacillus paracasei is the lactobacillus paracasei (Lacticaseibacillus paracasei) NHNK-633 which is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20251542 in the 07 th month of 2025. Further, the improvement of male reproductive health includes the production of L-citrulline, modulation of vascular endothelial cells, and improvement of prostatitis. Further, the modulating vascular endothelial cells comprises increasing survival of oxidatively damaged vascular endothelial cells ea.hy926. Further, the modulating vascular endothelial cells further comprises upregulating expression of a first related gene of oxidatively damaged vascular endothelial cells ea.hy926. Further, the first related genes of the vascular endothelial cells EA.hy926 include vascular endothelial growth factor gene VEGF, cyclooxygenase-2 gene COX2 and nitric oxide synthase gene iNOS. Further, the improving prostatitis comprises increasing the survival rate of the LPS-induced damaged prostate cells PC-3. Further, the ameliorating prostatitis further comprises down-regulating expression of a second related gene of lipopolysaccharide LPS-induced damaged prostate cell PC-3 inflammation. Further, the second related genes of the prostate cell PC-3 inflammation include interleukin-6 gene IL-6, interleukin-8 gene IL-8 and interleukin-1 beta gene IL-1 beta. Further, the cheese bacillus paracasei is a gram positive colony, is rod-shaped under a microscope, grows on an MRS flat plate, can form an opaque circular colony with white color and smooth and round surface, has neat edges, can uniformly grow in a turbid manner in an MRS liquid culture medium, and has white precipitate after long-term storage. Further, the product is prepared from a product which is a live bacterial, fermentation/secretion product of the cheese bacillus paracasei. The preservation number of the cheese bacillus paracasei NHNK-633 is CCTCC NO: M20251542. Experiments show that NHNK-633 has the functions of producing L-citrulline, repairing aged vascular endothelial cells, promoting vasodilation, repairing aged prostate cells and reducing prostatitis, and can be used for preparing products for improving male reproductive health. Description of biological preservation Chees