CN-121971513-A - Application of herba Begoniae Laciniatae in preparation of medicine for treating ovarian reserve hypofunction

Abstract

The invention discloses an application of a water lily flower water extract in preparing a medicine for treating ovarian reserve function (DECREASED OVARIAN RESERVE, DOR), belonging to the technical field of traditional Chinese medicine Yao medicine. The invention discovers for the first time that the water extract of the water lily can obviously improve the estrus cycle disorder of DOR mice, obviously raise AMH and E 2 levels of DOR mice, obviously lower FSH and LH levels, obviously lower the locking follicular rate of DOR mice and obviously raise the oocyte maturation rate, so that the water extract of the water lily can be used for treating the ovarian reserve hypofunction and preventing and treating the ovarian function injury caused by chemotherapy drugs.

Inventors

• Ruan Ziyun
• XIA MENG
• LI ZHENGDA
• HUANG XINXIN
• LIN HUADAN
• WANG XU
• LI TIAN

Assignees

• 广西中医药大学

Dates

Publication Date

20260505

Application Date

20260225

Claims (8)

1. 1. Application of herba Begoniae Laciniatae water extract in preparing medicine for treating ovarian failure is provided.
2. 2. Application of herba Begoniae Laciniatae water extract in preparing medicine for preventing and treating ovarian function injury caused by chemotherapy is provided.
3. 3. The use according to claim 2, wherein the chemotherapeutic agent used in the chemotherapy is cyclophosphamide or cisplatin.
4. 4. The use according to claim 1 or 2, wherein the water extract of water lily is a water decoction of water lily root.
5. 5. The use according to claim 1 or 2, wherein the water extract of water lily is a concentrate or extract obtained by concentrating a water decoction of water lily root.
6. 6. The use according to claim 1 or 2, wherein the water extract of water lily is a solid extract obtained by concentrating and drying a water decoction of water lily root.
7. 7. The application of claim 1 or 2, wherein the preparation method of the water lily extract comprises the steps of taking the water lily Shui Liangen, soaking the water lily in the mass ratio of 1:10-50 for 10-30 min, heating and boiling, extracting with slow fire for 20-60 min, filtering to obtain filtrate, repeating the steps for 1-2 times, combining the filtrates, and concentrating under reduced pressure.
8. 8. The use according to claim 7, wherein the preparation method of the water lily aqueous extract further comprises adding ethanol to the concentrated medicinal liquid under reduced pressure for precipitation to remove impurities.

Description

Application of herba Begoniae Laciniatae in preparation of medicine for treating ovarian reserve hypofunction Technical Field The invention belongs to the technical field of traditional Chinese medicine Yao medicine, in particular to traditional Chinese medicine Yao medicine for improving the ovarian reserve function, and particularly relates to application of a water lily water extract in preparation of a medicine for treating ovarian reserve function. Background About 15% of the world's population is affected by infertility, a significant global health problem [Sang Q, Ray P F, Wang L. Understanding the genetics of human infertility [J]. Science, 2023, 380(6641): 158-163.]. is an important topic with the search for assisted reproductive technology (assisted reproductive technology, ART) to increase the number of assisted pregnancy people, female reproductive aging infertility. One of the main clinical manifestations of ovarian aging is hypofunction of ovarian reserve (DIMINISHED OVARIAN RESERVE, DOR), which accounts for the increasing proportion of infertility year by year, up to 10% -30%, and the patient's age is in a decreasing trend and is very prone to develop premature ovarian failure [Jiao X, Meng T, Zhai Y, Zhao L, Luo W, Liu P, Qin Y. Ovarian reserve markers in premature ovarian insufficiency: within different clinical stages and different etiologies [J]. Frontiers in Endocrinology, 2021, 12:601752.]. The hypofunction of ovarian stores (DECREASED OVARIAN RESERVE, DOR) means that the hypofunction of ovaries [STEINER A Z, PRITCHARD D, STANCZYK F Z, et al. Association between biomarkers of ovarian reserve and infertility among older women of reproductive age [J]. JAMA, 2017, 318(14): 1367-1376.], caused by the reduction of oocyte quantity and/or quality of women of childbearing age is mainly represented by the change of sex hormone level, menstrual disorder, fertility decline, climacteric symptoms and the like, and the phenomenon gradually younger [HU S, XU B, JIN L. Perinatal outcome in young patients with diminished ovarian reserve undergoing assisted reproductive technology [J]. Fertil Steril, 2020, 114(1): 118-124.].DOR forms hidden trouble for reproductive health of women of childbearing age, and the appearance of perimenopausal-like syndrome and the like caused by the phenomenon also affects physical and mental health of women, so that the protection of the ovarian function of women is more important. DOR pathogenesis includes genetic, immunological, environmental, pharmaceutical and stress factors, etc., but pathogenesis is not yet defined [ He Ruifen, yang Yongxiu, liang Xiaolei. Development of animal model study of hypoovariasis [ J ]. Chinese laboratory animal journal, 2024, 32 (2): 254-259 ], and lack of effective clinical treatment. The yellow water lily is taken as a special plant in China, is a dry root of yellow water lily (Polygala fallax Hemsl) of polygala plants of polygala of Polygalaceae, is mainly produced in places such as Fujian, guangdong, guangxi, hunan, sichuan, yunnan and the like, and is collected and carried in Guangxi national medicine brief (Guangxi Zhuang Yao medicine quality Standard (Guangxi Yao medicine quality Standard) (Hunan Chinese medicinal material Standard (Guangdong province Chinese medicinal material Standard), and the like. The yellow flower-water lily is used as a traditional medicinal and edible plant in the south, is rich in various nutritional ingredients such as protein, amino acid, mineral elements and the like, is decocted into nourishing and beautifying soup, and is used for women with postpartum weakness and blood replenishing, and is characterized by good name [ Liao Suji, liang Jie, tang Chunfeng and the like of 'southern ginseng', 'yellow flower-ginseng', 'astragalus root', and the like. The herba polygoni multiflori is also a common medicinal material of minority nationality such as Zhuang nationality and Yao nationality, and is used for tonifying qi, strengthening tendons and bones, protecting liver, postpartum and sick weakness, anemia, kidney deficiency and lumbago, irregular menstruation and uterine prolapse. By searching the prior art, no literature report on the use of the water lily for improving the function of ovarian reserve or treating the hypofunction of ovarian reserve exists at present. Disclosure of Invention Aiming at the problems that more and more women have hypoovaries and infertility are more severe, the invention aims to provide the application of the water extract of the herba polygoni multiflori in preparing the medicine for treating the hypoovaries. In order to achieve the technical aim, the inventor finds that the water extract of the water lily has an effect of improving the ovarian function of mice with reduced ovarian reserve function through a large number of experimental researches based on the research experience of the Zhuang medicine for many years. The ovaries are very sensitive to toxicity of chemotherapeuti