CN-121971515-A - Preparation method and application of sauce head tuber alcohol extract

Abstract

The invention relates to the technical field of medicines, and discloses a preparation method and application of a sauce head tuberous root alcohol extract. The preparation method of the pure sauce head extract comprises the steps of crushing dry sauce head tuberous roots, sieving, weighing sauce head powder, adding 95% ethanol, soaking and extracting for 2d at room temperature, stirring once for 30min, carrying out suction filtration after soaking is finished to obtain ethanol leaching liquor, soaking and extracting filter residues for 2 times, combining the ethanol leaching liquor, transferring the ethanol leaching liquor into a rotary vacuum concentrator, concentrating and drying to obtain the alcohol extract of the sauce head tuberous roots, and experimental verification shows that the prepared pure sauce head extract has the effect of relieving fatty liver, so that the pure sauce head extract can be used for preparing medicines for relieving fatty liver.

Inventors

• CUI XIUMING
• LIU MIN
• MA JI
• CUI HAO

Assignees

• 昆明理工大学

Dates

Publication Date

20260505

Application Date

20260304

Claims (5)

1. 1. A preparation method of a sauce head (Pteroxygonum denticulatum) tuberous root alcohol extract is characterized by comprising the following steps: Pulverizing dry sauce head root tuber to obtain sauce head root tuber powder, soaking and extracting the sauce head root tuber powder with 95% ethanol by volume fraction, filtering to obtain ethanol extract, and concentrating the ethanol extract to obtain sauce head root tuber ethanol extract.
2. 2. The method for preparing the alcohol extract of the sauce head tubers, which is characterized in that the solid-to-liquid ratio of the sauce head tubers powder to the ethanol with the volume fraction of 95% is 1 (4-7), and the unit is g/mL.
3. 3. The method for preparing the alcoholic extract of sauce head tubers according to claim 1, wherein the number of soaking and extracting times is 1-3, and the time of each soaking and extracting is 1-4 days.
4. 4. The method for preparing the alcohol extract of the sauce head tubers according to claim 1, wherein the method comprises the following steps: the concentration method is evaporation concentration.
5. 5. The application of the alcohol extract of the sauced tuber prepared by the method of any one of claims 1-4 in preparing a medicament for relieving fatty liver.

Description

Preparation method and application of sauce head tuber alcohol extract Technical Field The invention relates to the technical field of medicines, in particular to a preparation method and application of a sauce head tuberous root alcohol extract. Background Fatty liver, medically known as liver steatosis, refers to a common liver disease with abnormal accumulation of fat in liver cells. Typically, an intrahepatic fat content of more than 5% by weight of the liver is diagnosed as fatty liver. It is not an independent disease, but rather a metabolic disorder in the liver caused by various factors such as obesity, excessive drinking, insulin resistance, high-fat diet, etc. Fatty liver is usually free of obvious symptoms in early stages, and most people are found accidentally through ultrasonic examination during physical examination. With the progress of the disease, symptoms such as hypodynamia, discomfort of the right upper abdomen and the like may appear gradually. Without intervention, simple fatty liver may progress to steatohepatitis, which may lead to liver fibrosis and cirrhosis, and even increase liver cancer risk. It is alarming that fatty liver has become one of the most common chronic liver diseases worldwide, and the onset of the disease presents a tendency to younger. The sauce head is a common medicine used by Yi nationality in Yunnan province and is derived from root tuber of Polygonum aviculare of Polygonum genus of Polygonaceae family. The sauce has astringent and cool properties, and has antiinflammatory, gastric ulcer resisting and antibacterial effects. Traditionally, sauce heads can be used for treating diarrhea, dyspepsia and other diseases by decoction or grinding and infusing. According to folk surveys, yi nationality herbal medicine of Chuxiong and Huize also often uses sauce heads, and particularly has obvious curative effects on treating dyspepsia and chronic hepatitis. Disclosure of Invention Aiming at the problems existing in the prior art, one of the purposes of the invention is to provide a preparation method of a sauce head (Pteroxygonum denticulatum) root tuber alcohol extract, which is characterized by comprising the following steps: Soaking and extracting the dried sauce head root tuber powder obtained by crushing the sauce head root tuber with ethanol with the volume fraction of 95%, filtering after the soaking and extraction are finished to obtain ethanol leaching liquor, and concentrating the ethanol leaching liquor to obtain the sauce head root tuber ethanol extract. Preferably, the solid-to-liquid ratio of the sauce head root tuber powder to the ethanol with the volume fraction of 95% is 1 (4-7), and the unit is g/mL. Preferably, the times of soaking and extracting are 1-3 times, and the time of each soaking and extracting is 1-4 days. Preferably, the concentration is by evaporation. The second purpose of the invention is to provide an application of the alcohol extract of the sauced tuber in preparing a medicine for relieving fatty liver. Compared with the prior art, the invention has the following beneficial effects: (1) The function is comprehensive, and the multi-target cooperative intervention is realized. The prior art scheme aims at a single pathological link (such as lipid lowering, anti-inflammatory or anti-fibrosis) of the non-alcoholic fatty liver disease, and is difficult to treat both principal and secondary aspect of the disease, and the invention proves that the sauce head tuberous root alcohol extract can perform systematic intervention from a disease source and a core process, so that the sauce head tuberous root alcohol extract not only can remarkably reduce liver triglyceride and total cholesterol, lighten liver steatosis, but also can synchronously inhibit inflammatory reaction, block and even reverse liver fibrosis process, and realize efficient and synergistic blocking on the malignant cycle of the core pathological feature of the fatty liver disease, namely lipid accumulation-inflammation-fibrosis. (2) Has dual values of root cause prevention and deep treatment. The invention discovers that the sauce head tuberous root alcohol extract not only can be used as a therapeutic agent to obviously improve the formed liver steatosis, inflammation and fibrosis, but also can play a role in preventing the occurrence and development of the metabolism-related fatty liver through the upstream and core links of targeted regulation of liver lipid metabolism and accumulation. The characteristic of 'prevention-treatment integration' ensures that the composition has unique and wide application prospect in the whole period management of diseases. (3) The natural source and the safety is expected to be good. The active ingredients of the invention are natural plant extracts, the characteristics of multicomponent synergistic effect and natural sources of the natural plant extracts, compared with most chemical synthetic medicines, the natural plant extracts are expected