CN-121971517-A - Traditional Chinese medicine Yao medicine for improving ovarian reserve function and application thereof

Abstract

The invention discloses a traditional Chinese medicine Yao medicine for improving an ovary reserve function and application thereof, and belongs to the technical field of traditional Chinese medicine Yao medicines. The invention discovers for the first time that the water extract of the purple nine cows can obviously improve the estrus cycle disorder of the DOR mice, obviously raise the AMH and E 2 levels of the DOR mice, obviously lower the FSH and LH levels, obviously lower the locking follicular rate of the DOR mice and obviously raise the oocyte maturation rate, so that the water extract of the purple nine cows can be used for treating the ovarian reserve function decline and preventing and treating the ovarian function injury caused by chemotherapeutics.

Inventors

• Ruan Ziyun
• LI ZHENGDA
• XIA MENG
• LI TIAN
• WEN YINGHUA
• HUANG XINXIN
• LIN HUADAN
• WANG XU

Assignees

• 广西中医药大学

Dates

Publication Date

20260505

Application Date

20260226

Claims (8)

1. 1. Application of radix Ardisiae Japonicae water extract in preparing medicine for treating ovarian reserve hypofunction is provided.
2. 2. Application of radix Ardisiae Japonicae water extract in preparing medicine for preventing and treating ovarian function injury caused by chemotherapy is provided.
3. 3. The use according to claim 2, wherein the chemotherapeutic agent used in the chemotherapy is cyclophosphamide or cisplatin.
4. 4. Use according to claim 1 or 2, wherein the water extract of the plant kapok is a water decoction of the roots and/or rhizomes of the plant kapok of the family rhamnaceae.
5. 5. The use according to claim 1 or 2, wherein the water extract of the plant kava is a concentrate or extract obtained by concentrating the water decoction of the roots and/or rhizomes of the plant kava.
6. 6. The use according to claim 1 or 2, wherein the water extract of the plant kapok is a solid extract obtained by concentrating and drying a water decoction of roots and/or rhizomes of the plant kapok of the family rhamnaceae.
7. 7. The application of claim 1 or 2, wherein the preparation method of the purple-nine-cow aqueous extract comprises the steps of taking roots and/or rhizomes of Rhamnaceae plant winged drupes, soaking in water for 10-30 min according to a mass ratio of 1:10-50, heating and boiling, extracting with slow fire for 20-60 min, filtering to obtain filtrate, repeating the steps for 1-2 times, combining the filtrates, and concentrating under reduced pressure.
8. 8. The method according to claim 7, wherein the method for preparing the water extract of Bos taurus elaidicus further comprises adding ethanol into the concentrated medicinal liquid under reduced pressure for precipitation to remove impurities.

Description

Traditional Chinese medicine Yao medicine for improving ovarian reserve function and application thereof Technical Field The invention belongs to the technical field of traditional Chinese medicine Yao medicine, in particular to traditional Chinese medicine Yao medicine for improving the ovarian reserve function, and particularly relates to application of a purple nine-cow water extract in preparation of a medicine for improving the ovarian reserve function. Background About 15% of the world's population is affected by infertility, a significant global health problem [Sang Q,Ray P F,Wang L. Understanding the genetics of human infertility [J]. Science, 2023, 380(6641): 158-163.]. is an important topic with the search for assisted reproductive technology (assisted reproductive technology, ART) to increase the number of assisted pregnancy people, female reproductive aging infertility. One of the main manifestations of ovarian aging in clinic is hypofunction of ovarian reserve (DIMINISHED OVARIAN RESERVE, DOR), which is a proportion of infertility that rises up to 10% -30% year by year, and the age of patients is a decreasing trend, and is very easy to develop premature ovarian failure [Jiao X, Meng T, Zhai Y, Zhao L, Luo W, Liu P, Qin Y. Ovarian reserve markers in premature ovarian insufficiency: within different clinical stages and different etiologies [J]. Frontiers in Endocrinology, 2021, 12:601752.]. while the unmarried proportion of women aged 35 years and older in China is obviously rising, from 0.24% in 1982 to 1.20% in 2020 [ Yang Juhua, li Yanyu, wang Susu ], the development status of the subthreshold female population for high quality development of population, problem and countermeasure research-analysis based on seven national population census data [ J ]. Chinese population science, 2025,39 (1): 7-22 ]. The hypofunction of ovarian stores (DECREASED OVARIAN RESERVE, DOR) means that the hypofunction of ovaries [STEINER A Z, PRITCHARD D,STANCZYK F Z, et al. Association between biomarkers of ovarian reserve and infertility among older women of reproductive age [J]. JAMA, 2017, 318(14): 1367-1376.], caused by the reduction of oocyte quantity and/or quality of women of childbearing age is mainly represented by the change of sex hormone level, menstrual disorder, fertility decline, climacteric symptoms and the like, and the phenomenon gradually younger [HU S, XU B, JIN L. Perinatal outcome in young patients with diminished ovarian reserve undergoing assisted reproductive technology [J]. Fertil Steril, 2020, 114(1): 118-124.].DOR forms hidden trouble for reproductive health of women of childbearing age, and the appearance of perimenopausal-like syndrome and the like caused by the phenomenon also affects physical and mental health of women, so that the protection of the ovarian function of women is more important. DOR pathogenesis includes genetic, immunological, environmental, pharmaceutical and stress factors, etc., but pathogenesis is not yet defined [ He Ruifen, yang Yongxiu, liang Xiaolei. Development of animal model study of hypoovariasis [ J ]. Chinese laboratory animal journal, 2024, 32 (2): 254-259 ], and lack of effective clinical treatment. The purple nine cattle is one of traditional Yao medicine in China, and is derived from dry roots and rhizomes of Rhamnaceae plant Rhamnus verniciflua (Ventilago leiocarpa benth.) and mainly distributed in Guangdong, guangxi and Yunnan areas in China. The Chinese medicinal composition is characterized by being prepared from caulis seu radix Kadsurae Longipedunculatae, radix Ardisiae Crispae, caulis et folium Hedyotideae sinensis, niu Can and the like, is originally carried in Guangxi Chinese herbal medicine, is a warm and sweet medicinal material, belongs to the so-called "Feng-Yao medicine" of Yao medicine, has the effects of tonifying qi and blood, strengthening tendons and bones, relaxing tendons and collaterals and the like (Liu Qianqian, liang Xiaolian, li Wenli and the like; the current research situation of the national medicine red radix Ardisiae Crispae [ J ]. Hubei agricultural science, 2021,60 (16): 5-8.), and is commonly used for treating symptoms such as qi and blood weakness, irregular menstruation, blood deficiency amenorrhea, rheumatalgia, traumatic injury, lumbar muscle strain, limb numbness and the like in folk. By searching and analyzing the prior art, no document report on using the purple-nine cattle for improving the function of the ovarian reserve or treating the hypofunction of the ovarian reserve exists at present. Disclosure of Invention In view of the defects of the prior art, the invention aims to provide a traditional Chinese medicine Yao medicine for improving the ovarian reserve function so as to solve the problems that more females have the ovarian reserve function reduced and the infertility situation is more severe. In order to achieve the technical aim, the inventor finds that the water extract of the purple nine cattle has