CN-121971520-A - Method for preparing gastrointestinal tract health care traditional Chinese medicine for animals by fermentation

CN121971520ACN 121971520 ACN121971520 ACN 121971520ACN-121971520-A

Abstract

The invention discloses a method for preparing animal gastrointestinal tract health care traditional Chinese medicine fermentation in the technical field of animal feed additives, which comprises the steps of crushing traditional Chinese medicines such as astragalus, poria cocos, bighead atractylodes rhizome, hawthorn, dried orange peel and liquorice, mixing with wheat bran, adding composite probiotics and a unique dynamic covalent bonding plant polysaccharide polyphenol three-dimensional reticular carrier material fine powder, carrying out constant-temperature fermentation after humidity adjustment, and finally drying and crushing to obtain the product. The key point is that the carrier material is assembled by oxidized sodium alginate, hydrazide carboxymethylated yeast glucan and tea polyphenol tannic acid composite nano colloid through the synergistic effect of dynamic covalent bonds and hydrogen bonds. The material not only can remarkably improve the survival rate and metabolic activity of probiotics in a fermentation system and promote the conversion of active ingredients of traditional Chinese medicines, but also has intelligent responsiveness, and can realize targeted slow release in animal intestinal tracts, thereby comprehensively enhancing the gastrointestinal tract health care function of the product and improving the growth performance of animals.

Inventors

ZHANG JING
XIAO JUHUA
LI XUEJUN
YANG HUAN

Assignees

湖南飞力格科技有限公司

Dates

Publication Date: 20260505
Application Date: 20251224

Claims (10)

1. A method for preparing gastrointestinal tract health care traditional Chinese medicine for animals by fermentation is characterized by comprising the following steps: s1, mixing 25-35 parts of astragalus, 15-25 parts of poria cocos, 14-16 parts of bighead atractylodes rhizome, 14-16 parts of hawthorn, 8-12 parts of dried orange peel and 8-12 parts of liquorice, and then crushing the mixture into powder by using a crusher to obtain traditional Chinese medicine matrix powder; S2, grinding and sieving the dynamic covalent bonding plant polysaccharide-polyphenol three-dimensional network carrier material to obtain dynamic covalent bonding plant polysaccharide-polyphenol three-dimensional network carrier material fine powder, and placing the traditional Chinese medicine-wheat bran mixture, the composite probiotic freeze-dried powder and the dynamic covalent bonding plant polysaccharide-polyphenol three-dimensional network carrier material fine powder into a sterile mixer in a sterile operation table for mixing to obtain a mixed material; S3, spraying sterile deionized water into the mixed materials, stirring to obtain a material subjected to humidity regulation, filling the material subjected to humidity regulation into a fermentation bag, compacting, sealing, placing in a constant-temperature incubator, and standing and fermenting at 36-38 ℃ to obtain a fermentation product; s4, drying the fermentation product in a hot air circulation oven at 48-52 ℃ to obtain a dried product, and crushing and sieving the dried product.
2. The method for preparing a gastrointestinal tract health-care traditional Chinese medicine for animals according to claim 1, wherein in the step S1, the mass ratio of the traditional Chinese medicine matrix powder to the wheat bran is 7:3.
3. The method for preparing the gastrointestinal tract health care traditional Chinese medicine for animals according to claim 1, wherein in the step S2, the composite probiotics freeze-dried powder is prepared by mixing lactobacillus plantarum, bacillus subtilis and candida utilis according to the viable count of 5:3:2.
4. The method for preparing a gastrointestinal tract health-care traditional Chinese medicine for animals according to claim 1, wherein in the step S3, the standing fermentation time is 72-80h at 36-38 ℃.
5. The method for preparing a gastrointestinal tract health-care traditional Chinese medicine for animals according to claim 1, wherein in the step S4, the traditional Chinese medicine is sieved by a 40-42 mesh sieve.
6. The method for preparing the gastrointestinal tract health care traditional Chinese medicine for animals according to any one of claims 1 to 5, wherein the preparation step of the dynamic covalent bonding plant polysaccharide-polyphenol three-dimensional network carrier material comprises the following steps: Under the condition of keeping away light, sodium alginate is dissolved in deionized water to obtain a homogeneous solution, under the condition of stirring and 0-4 ℃, sodium periodate is dripped into the homogeneous solution to be dissolved in the deionized water to obtain a reaction mixture, the reaction mixture is placed at 24-26 ℃ and is stirred for reaction in the absence of light, ethylene glycol is added, stirring is continued to obtain a reaction solution, the reaction solution is transferred into a dialysis bag for dialysis to obtain a dialyzed liquid, and the dialyzed liquid is freeze-dried to obtain oxidized sodium alginate; A2, dispersing yeast beta-glucan in a sodium hydroxide aqueous solution, alkalifying at 45-55 ℃, adding sodium monochloroacetate, reacting at 55-65 ℃, neutralizing with glacial acetic acid after the reaction is finished, precipitating with ethanol solution, washing, drying to obtain carboxymethylated yeast beta-glucan, dissolving the carboxymethylated yeast beta-glucan in a mixed solvent of deionized water and anhydrous dimethyl sulfoxide, sequentially adding adipic acid dihydrazide, 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide under nitrogen protection and stirring, reacting at 24-26 ℃ to obtain a reaction solution, dialyzing the reaction solution in a dialysis bag to obtain a dialyzate, and freeze-drying the dialyzate to obtain hydrazidated carboxymethylated yeast beta-glucan; A3, dissolving epigallocatechin gallate and tannic acid in deionized water at 60-70 ℃ together, stirring to obtain a solution, injecting acetone precooled to 2-6 ℃ into the solution at 60-70 ℃ to obtain a mixture, standing and aging the mixture at 3-5 ℃, centrifuging at 3-5 ℃, collecting precipitate, redispersing the precipitate in phosphate buffer solution with pH of 7.2-7.6, and filtering to obtain tea polyphenol-tannic acid composite nano colloid suspension; A4, dissolving oxidized sodium alginate in acetic acid-sodium acetate buffer solution with pH of 4.8-5.2 at room temperature to obtain solution A, dissolving hydrazide carboxymethylated yeast beta-glucan in phosphate buffer solution with pH of 7.0-7.5 to obtain solution B, adding the solution B into the solution A under stirring to obtain mixed solution, adding tea polyphenol-tannic acid composite nano colloid suspension into the mixed solution under stirring, continuing stirring to obtain reaction solution, regulating the pH value of the reaction solution to 6.6-7.0 to obtain primary wet gel, placing the primary wet gel in a mould, standing for solidification at 3-5 ℃, and finally freeze-drying.
7. The method for preparing the gastrointestinal tract health care traditional Chinese medicine for animals according to claim 6, wherein in the step A1, the reaction time is 6-8h after light-shielding stirring.
8. The method for preparing a gastrointestinal tract health-care traditional Chinese medicine for animals according to claim 6, wherein in the step A2, the reaction time is 3-5h at 55-65 ℃.
9. The method for preparing a gastrointestinal tract health-care traditional Chinese medicine for animals according to claim 6, wherein in the step A3, the centrifugation time is 30-60min at 3-5 ℃.
10. The method for preparing a gastrointestinal tract health-care traditional Chinese medicine for animals according to claim 6, wherein in the step A4, the standing and solidifying time is 24-30h at 3-5 ℃.

Description

Method for preparing gastrointestinal tract health care traditional Chinese medicine for animals by fermentation Technical Field The invention belongs to the technical field of animal feed additives, and particularly relates to a fermentation preparation method of gastrointestinal tract health care traditional Chinese medicine for animals. Background With the gradual disablement of antibiotic additives in feeds worldwide, the livestock breeding industry faces a great challenge of maintaining animal health and guaranteeing production performance. In this context, the development of safe, efficient, green alternative products is the core direction of industry research. The traditional Chinese medicine is natural in origin, has multiple effects of resisting bacteria and diminishing inflammation, regulating immunity, promoting digestion and the like, has unique advantages in improving the gastrointestinal health of animals, and is considered as one of potential options for replacing antibiotics. However, the traditional Chinese medicine additive is usually prepared by directly adding medicinal material powder into feed, the palatability is often poor, and macromolecular active ingredients in the medicinal material are difficult to be directly and efficiently absorbed by animal intestinal tracts, so that the bioavailability is low, the use effect is unstable, the dosage is large, and the economic benefit is limited. In order to break through the bottleneck, the traditional Chinese medicine treatment by using the modern microbial fermentation technology becomes an effective path. Through the metabolism of probiotics, macromolecular substances such as cellulose, starch and the like in medicinal materials can be decomposed, and some complex glycoside components are converted into aglycone which is easier to absorb, and meanwhile, beneficial metabolites such as organic acid, enzymes and the like are generated, so that the functional value of the traditional Chinese medicine formula is comprehensively improved. At present, research and practice in the field have advanced to a certain extent, and a new solution is provided for green cultivation. Although the technology of fermenting traditional Chinese medicines by probiotics shows good prospects, the technology still has a plurality of key bottlenecks in deep popularization and efficacy maximization. First, the efficiency and stability of the fermentation system is highly dependent on the viability of the probiotics used. In a complex environment of solid state fermentation, probiotics face multiple pressures such as nutrition competition, metabolite inhibition and the like, and the number of viable bacteria is easy to be reduced in the middle and later stages of fermentation, so that the fermentation depth and the quality of the final product are affected. More importantly, after the fermentation product is fed to animals, probiotics and active ingredients in the fermentation product need to pass through a stomach environment with extremely strong acidity to reach intestinal tracts to exert efficacy, a large amount of thalli are inactivated in the process, and functional ingredients are destroyed, so that the actual effect is greatly reduced. The existing technical schemes focus on strain compatibility optimization or fermentation process parameter adjustment, such as temperature adjustment, humidity adjustment and fermentation duration adjustment, and although the methods have certain improvements, the survival challenges of probiotics in the fermentation process and in-vivo delivery cannot be fundamentally solved. In addition, some researches try to immobilize strains by using common carriers, but the protection effect is limited, intelligent responsiveness is lacking, and the accurate release of active substances at specific parts of intestinal tracts cannot be realized. Therefore, how to construct an intelligent system capable of improving fermentation efficiency and realizing intestinal targeted delivery is a breakthrough difficulty in the current technology. In recent years, the development of material science, especially in the fields of dynamic covalent chemistry and functional polymers, provides a new idea for solving the problems. Dynamic covalent chemistry refers to the construction of molecular structures by utilizing reversible broken and recombined covalent bonds, and polymer materials developed based on the principle have intelligent characteristics such as environmental response, self-repair and the like. For example, the structural stability and dissociation behavior of hydrogel networks constructed based on dynamic acylhydrazone bonds can change with changes in environmental ph. Meanwhile, natural polysaccharides and polyphenols are widely used for constructing drug delivery vehicles due to their good biocompatibility, modifiable properties and unique bioactivity. Researchers have utilized these natural molecules to build three-dimensional network