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CN-121971538-A - Muscle anti-aging composition combining spermidine and wolfberry water extract and application thereof

CN121971538ACN 121971538 ACN121971538 ACN 121971538ACN-121971538-A

Abstract

The invention discloses a muscle anti-aging composition combining spermidine and a medlar water extract and application thereof, belonging to the technical field of application of bioactive substances. The composition is prepared by compounding spermidine and a medlar water extract according to a specific proportion, wherein the medlar water extract is prepared by an optimized water extraction process, and core active ingredients are reserved to a greater extent. The optimal ratio of spermidine to the wolfberry fruit water extract is 1:2 of the mass ratio of the mother solution. The invention obviously improves the antioxidant capacity of aging muscle cells through a synergistic action mechanism, and effectively inhibits the oxidative stress and aging process of the D-galactose-induced muscle cells. The composition has simple preparation process and safe components, can be widely applied to the fields of foods, health products and medicines, and provides a new solution for developing efficient and natural muscle anti-aging products.

Inventors

  • LI YAN
  • TANG YUNDI
  • WANG YIHAN
  • WANG YIWU
  • WANG JINYI
  • YANG ZINING
  • An Shuozhen
  • WANG LI

Assignees

  • 江南大学

Dates

Publication Date
20260505
Application Date
20260316

Claims (10)

  1. 1. The muscle anti-aging composition is characterized by comprising spermidine and a wolfberry water extract, wherein the mass ratio of the spermidine to the wolfberry water extract is 2:1-1:3.
  2. 2. The muscle anti-aging composition of claim 1, wherein the mass ratio of spermidine to the aqueous extract of lycium barbarum is 1:2.
  3. 3. The muscle anti-aging composition of claim 1, wherein the process for preparing the aqueous extract of lycium barbarum comprises the steps of: (1) Cleaning and soaking, namely weighing dried fruits of Chinese wolfberry, removing impurities, cleaning, soaking in water to enable the pulp of the Chinese wolfberry to fully absorb water and expand; (2) Heat extraction, namely placing the mixture of the medlar soaked in the step (1) and water into a water bath kettle for water bath treatment to promote dissolution of water-soluble active ingredients in the medlar; (3) Ultrasonic assisted extraction, namely transferring the mixture obtained in the step (2) after heat extraction into ultrasonic equipment for ultrasonic treatment to obtain an extracting solution; (4) And (3) centrifugal separation, namely, carrying out centrifugal treatment on the extracting solution obtained in the step (3), concentrating the obtained supernatant, and freeze-drying to obtain the medlar water extract.
  4. 4. The muscle anti-aging composition according to claim 1, wherein the soaking in water in the step (1) is performed at 18-20 ℃ for 25-30 min, and the mass ratio of medlar to water is 1:5-20.
  5. 5. The muscle anti-aging composition according to claim 1, wherein the water bath temperature in step (2) is 70 to 90 ℃ and the water bath time is 40 to 80 minutes.
  6. 6. The muscle anti-aging composition according to claim 1, wherein the ultrasonic power in step (3) is 240 to 300w, the ultrasonic temperature is 40 to 60 ℃, and the ultrasonic time is 20 to 40min.
  7. 7. The muscle anti-aging composition according to claim 1, wherein the centrifugation in step (4) is performed at 4000 to 6000rpm for 8 to 15 minutes, and the concentration is performed by a rotary evaporator at 45 ℃ to 1/2 of the original volume.
  8. 8. The muscle anti-aging composition according to claim 1, wherein the lyophilization in step (4) is divided into five stages, namely, a first stage of-30 ℃ and 720min, a second stage of-20 ℃ and 720min, a third stage of-10 ℃ and 600min, a fourth stage of 0 ℃ and 480min, and a fifth stage of 5 ℃ and 9999min. .
  9. 9. Use of the muscle anti-aging composition of any one of claims 1 to 8 in the preparation of muscle anti-aging products, including anti-aging related foods, health products and pharmaceuticals.
  10. 10. The use according to claim 9, comprising inhibiting oxidative stress in senescent muscle cells, increasing cellular SOD and CAT activity, reducing MDA content, and reducing oxidative damage marker levels using the muscle anti-aging composition.

Description

Muscle anti-aging composition combining spermidine and wolfberry water extract and application thereof Technical Field The invention relates to the technical field of application of bioactive substances, in particular to a muscle anti-aging composition of spermidine combined with a medlar water extract and a preparation method thereof, which are particularly suitable for improving the problems of oxidative stress and the like related to muscle cell aging. Background As the global population ages, the incidence of sarcopenia (sarcopenia) and related degenerative diseases continues to rise. The decrease in muscle mass and the decline in function have become one of the central factors threatening the quality of life of the elderly population. Modern research has shown that muscle aging is a multifactorial driven complex process with core mechanisms including imbalance in redox homeostasis, decline in muscle stem cell function, decline in autophagic activity, and disturbance in polyamine metabolism. These factors interact, resulting in impaired proliferation and differentiation of muscle cells, increased apoptosis, and ultimately, irreversible degeneration of muscle structure and function. In this context, the search for safe and effective anti-aging active ingredients from natural products has become a research hotspot. Spermidine, a naturally occurring polyamine-based compound, has been shown to have significant anti-aging effects in recent years. Studies show that spermidine can activate muscle stem cells by regulating spermidine-eIF 5A-MyoD signal axis, induce autophagy, improve oxidative stress state in cells and maintain polyamine steady state, so that aging process of muscle cells is delayed. Meanwhile, the medlar serving as a traditional medicine and food dual-purpose plant is rich in a plurality of active ingredients such as medlar polysaccharide, polyphenol, flavone and the like, and has broad-spectrum antioxidation and anti-inflammatory effects. Researches show that the medlar extract can effectively remove excessive free radicals, relieve muscle cell injury induced by oxidative stress, and has good muscle protection potential. Although each of spermidine and wolfberry extract has made some progress in anti-aging studies, the current state of the art is focused mainly on the application of a single active ingredient. The application of single components often faces the problems of limited effect intensity, single action path and the like, and the comprehensive intervention on the muscle aging multi-target mechanism is difficult to realize. In addition, the existing preparation process of the medlar extract mainly aims at extracting a single active ingredient, and the influence of process parameters on anti-aging activity is not researched systematically, so that the application potential of the medlar extract in a composite preparation is limited. Therefore, how to scientifically combine natural active ingredients with complementary effects based on a multi-target mechanism of muscle aging and optimize an extraction process to obtain synergy is still a technical problem to be solved in the field. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a muscle anti-aging composition of spermidine combined with a medlar water extract, which realizes the remarkable synergy of spermidine and medlar water extract in muscle anti-aging aspect by optimizing the preparation process of the medlar water extract and screening the optimal proportion of spermidine and medlar water extract, and solves the problems of limited effect of single component and single action mechanism. In order to achieve the above purpose, the invention firstly provides a muscle anti-aging composition of spermidine combined with a medlar water extract, wherein the composition comprises spermidine and the medlar water extract, and the mass ratio of the spermidine to the medlar water extract is 2:1-1:3, preferably 1:2. In one embodiment of the invention, the preparation process of the medlar water extract comprises the following steps: (1) Cleaning and soaking, namely weighing dried fruits of Chinese wolfberry, removing impurities, cleaning, soaking in water to enable the pulp of the Chinese wolfberry to fully absorb water and expand; (2) Heat extraction, namely placing the mixture of the medlar soaked in the step (1) and water into a water bath kettle for water bath treatment to promote dissolution of water-soluble active ingredients in the medlar; (3) Ultrasonic assisted extraction, namely transferring the mixture obtained in the step (2) after heat extraction into ultrasonic equipment for ultrasonic treatment to obtain an extracting solution; (4) And (3) centrifugal separation, namely, carrying out centrifugal treatment on the extracting solution obtained in the step (3), concentrating the obtained supernatant, and freeze-drying to obtain the medlar water extract. In one embodiment of the invention, the