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CN-121971553-A - Polygonatum sibiricum flower bud extract and preparation method thereof, microcapsule preparation and application

CN121971553ACN 121971553 ACN121971553 ACN 121971553ACN-121971553-A

Abstract

The invention belongs to the technical field of natural medicine extracts, and relates to a polygonatum sibiricum flower bud extract, a preparation method thereof, a microcapsule preparation and application thereof. The Polygonatum sibiricum flower bud extract contains one or more of rutin, apigenin-6-arabinose-8-glucoside and apigenin-6, 8-diglucoside. The preparation method of the polygonatum sibiricum flower bud extract and the preparation method of the microcapsule preparation can be used for respectively preparing the polygonatum sibiricum flower bud extract and the microcapsule preparation of the polygonatum sibiricum flower bud extract, and the prepared polygonatum sibiricum flower bud extract and the microcapsule preparation of the polygonatum sibiricum flower bud extract can better inhibit the activity of alpha-glucosidase in vitro and in vivo, and can be used for preventing or treating diabetes with better curative effect and smaller side effect.

Inventors

  • WANG HAIFENG

Assignees

  • 北京煜坤盛科技发展有限公司

Dates

Publication Date
20260505
Application Date
20260227

Claims (10)

  1. 1. The polygonatum sibiricum flower bud extract is characterized by comprising one or more of rutin, apigenin-6-arabinose-8-glucoside and apigenin-6, 8-diglucoside.
  2. 2. The method for preparing the extract of the flower buds of polygonatum sibiricum according to claim 1, wherein the preparation method comprises the following steps: (1) Collecting buds of Polygonatum sibiricum, and performing pretreatment; (2) Carrying out ultrasonic extraction on the pretreated polygonatum sibiricum flower buds in an ethanol solution; (3) Concentrating and drying the ultrasonic extract to obtain solid powder of the polygonatum sibiricum flower bud extract.
  3. 3. The preparation method according to claim 2, characterized in that: In the step (1), the pretreatment comprises cleaning, impurity removing, degreasing, drying, crushing, and/or In the step (2), the volume percentage concentration of the ethanol solution is 50-60%, the feed liquid ratio of the polygonatum sibiricum flower buds to the ethanol solution is 1:20-30 g/mL, the ultrasonic extraction temperature is 52-57 ℃, the ultrasonic extraction power is 200-250W, the ultrasonic extraction time is 1-3h, the ultrasonic extraction times are 1-3 times, and the ultrasonic extraction solutions are combined.
  4. 4. The preparation method according to claim 2, characterized in that: In the step (3), the ultrasonic extracting solution is centrifugated, concentrated by a supernatant rotary evaporator, and then vacuum freeze-dried to obtain solid powder of the polygonatum sibiricum flower bud extract and/or And (3) after the step (3), separating the solid powder of the polygonatum sibiricum flower bud extract by macroporous adsorption resin.
  5. 5. The microcapsule preparation of the polygonatum sibiricum flower bud extract is characterized in that the microcapsule preparation takes the polygonatum sibiricum flower bud extract as a core material and whey protein and maltodextrin as wall materials, and the mass ratio of the polygonatum sibiricum flower bud extract to the whey protein to the maltodextrin is 2.25:8:1.
  6. 6. The method of preparing a microcapsule formulation according to claim 5, comprising the steps of: (1) Respectively preparing whey protein stock solution, maltodextrin stock solution and ethanol solution of Polygonatum sibiricum flower bud extract; (2) The microcapsule preparation is prepared by using the whey protein stock solution, the maltodextrin stock solution and the ethanol solution of the polygonatum sibiricum flower bud extract.
  7. 7. The process according to claim 6, wherein in the step (1), Adding water into whey protein, ultrasonic dispersing to obtain 3-5% whey protein stock solution, and/or Adding water into maltodextrin, stirring and dissolving to obtain maltodextrin stock solution with mass percentage concentration of 1.5-2.5%, and/or Adding ethanol into the Polygonatum sibiricum flower bud extract, stirring and dissolving to obtain an ethanol solution of the Polygonatum sibiricum flower bud extract with the mass percentage concentration of 5-10%.
  8. 8. The method of claim 6, wherein in step (2), the ethanol solution of the bud extract of Polygonatum sibiricum Red is dispersed in the stock solution of whey protein, the solvent is removed after ultrasonic treatment, then the maltodextrin stock solution is added, the mixture is stirred for a certain period of time and then is oscillated by a vortex oscillator, the mixture is kept stand for a certain period of time and centrifuged to obtain precipitate, and after washing with water and centrifugal impurity removal, the solid is collected and freeze-dried to obtain the powder of the microcapsule preparation.
  9. 9. Use of the extract of the flower buds of polygonatum sibiricum according to claim 1 or the microcapsule preparation of the extract of the flower buds of polygonatum sibiricum according to claim 5 for preparing a medicament for inhibiting the activity of alpha-glucosidase.
  10. 10. Use of the extract of the flower buds of polygonatum sibiricum according to claim 1 or the microcapsule preparation of the extract of the flower buds of polygonatum sibiricum according to claim 5 for preparing a medicament for inhibiting the activity of alpha-glucosidase, thereby preparing a medicament for preventing or treating diabetes.

Description

Polygonatum sibiricum flower bud extract and preparation method thereof, microcapsule preparation and application Technical Field The invention belongs to the technical field of natural medicine extracts, and relates to a polygonatum sibiricum flower bud extract, a preparation method thereof, a microcapsule preparation and application thereof. Background Diabetes is a major chronic metabolic disease in the world today, and since 1990, diabetes has increased four times the number of worldwide onset, currently suffering from as many as 8.3 million people, with type II diabetes being the vast majority. How to control postprandial and basal blood sugar of type II diabetics is a key to the treatment of diabetes, wherein the inhibition of alpha-glucosidase activity becomes an important clinical treatment target, but the existing hypoglycemic drugs aiming at the target can generate liver and kidney injury after long-term administration, and are accompanied with side effect manifestations such as abdominal distension, diarrhea, vomiting and the like. Therefore, the search for natural, side-effect-free hypoglycemic compounds has been the focus of the field of diabetes research. The polygonatum sibiricum (Polygonatum sibiricum) is used as a medicine and food dual-purpose resource in China, contains abundant active ingredients and has the functions of reducing blood sugar and relieving diabetic complications, however, a plurality of researches are concentrated on rhizomes at present, researches on non-traditional medicinal parts of the polygonatum sibiricum are freshly reported, and particularly flower buds with considerable biomass and edible performance lack of deep system research, so that standardized application of the polygonatum sibiricum is severely restricted. Therefore, it is necessary to develop the research of the extract of the bud of Polygonatum sibiricum Red for the treatment of diabetes. Disclosure of Invention The primary purpose of the invention is to provide the polygonatum sibiricum flower bud extract, so that the activity of alpha-glucosidase can be better inhibited in vivo and in vitro, the curative effect is better, and the side effect is smaller, so that the polygonatum sibiricum flower bud extract can be used for preventing or treating diabetes. To achieve the object, in a basic embodiment, the present invention provides a polygonatum sibiricum flower bud extract containing one or more of rutin, apigenin-6-arabinose-8-glucoside, apigenin-6, 8-diglucoside. The second object of the present invention is to provide a method for preparing the bud extract of Polygonatum sibiricum Red, as described above, so that the bud extract of Polygonatum sibiricum Red can be prepared better, the activity of alpha-glucosidase can be inhibited in vivo and in vitro, the curative effect is better, and the side effects are less, so that the bud extract of Polygonatum sibiricum Red can be used for preventing or treating diabetes. To achieve the object, in a basic embodiment, the present invention provides a method for preparing a bud extract of polygonatum sibiricum, as described above, comprising the steps of: (1) Collecting buds of Polygonatum sibiricum, and performing pretreatment; (2) Carrying out ultrasonic extraction on the pretreated polygonatum sibiricum flower buds in an ethanol solution; (3) Concentrating and drying the ultrasonic extract to obtain solid powder of the polygonatum sibiricum flower bud extract. In a preferred embodiment, the present invention provides a method for preparing the extract of flower buds of Polygonatum sibiricum Red as described above, wherein: In the step (1), the pretreatment comprises cleaning, impurity removing, degreasing, drying, crushing, and/or In the step (2), the volume percentage concentration of the ethanol solution is 50-60%, the feed liquid ratio of the polygonatum sibiricum flower buds to the ethanol solution is 1:20-30 g/mL, the ultrasonic extraction temperature is 52-57 ℃, the ultrasonic extraction power is 200-250W, the ultrasonic extraction time is 1-3h, the ultrasonic extraction times are 1-3 times, and the ultrasonic extraction solutions are combined. In a preferred embodiment, the present invention provides a method for preparing the extract of flower buds of Polygonatum sibiricum Red as described above, wherein: In the step (3), the ultrasonic extracting solution is centrifugated, concentrated by a supernatant rotary evaporator, and then vacuum freeze-dried to obtain solid powder of the polygonatum sibiricum flower bud extract and/or And (3) after the step (3), separating the solid powder of the polygonatum sibiricum flower bud extract by macroporous adsorption resin. The third object of the present invention is to provide a microcapsule preparation of the bud extract of Polygonatum sibiricum Red, as described above, to be able to better inhibit the activity of alpha-glucosidase in vivo and in vitro, and to be able to be used for the prevention or treatment