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CN-121971587-A - Immune homeostasis supporting agent based on mushroom active ingredients and fungus polysaccharide

CN121971587ACN 121971587 ACN121971587 ACN 121971587ACN-121971587-A

Abstract

The invention discloses an immune steady-state supporting agent based on fungus mushroom active ingredients and fungus polysaccharide, which relates to the technical field of biological medicines and functional foods, and comprises the following steps of preparing a pleurotus citrinopileatus steady-state adjusting extracting solution, leaching pleurotus citrinopileatus fruiting body powder with water at 80-100 ℃ for 30-60 minutes according to the ratio of feed liquid to 1 g:35-45 mL, so as to obtain a pleurotus citrinopileatus Huang Gujin extracting solution; the elm Huang Gujin extract is subjected to reduced pressure concentration and decolorization in sequence to obtain the elm yellow mushroom steady-state regulation extract with the concentration of 0.5-2 g/mL, and the elm yellow mushroom steady-state regulation extract shows bidirectional regulation characteristics in an experimental model, so that the immune function index in an immunosuppression state can be improved, and excessive immune response can be relieved, so that an immune system is guided and maintained in a balanced and stable optimal state.

Inventors

  • ZHENG MINJUN

Assignees

  • 深圳阳天生物科技有限责任公司

Dates

Publication Date
20260505
Application Date
20260120

Claims (8)

  1. 1. An immune homeostasis supporting agent based on mushroom active ingredients and fungus polysaccharide, comprising a preparation method, characterized in that the method comprises the following steps: (1) Preparing a pleurotus citrinopileatus steady state adjustment extracting solution, namely leaching pleurotus citrinopileatus fruiting body powder with water at 80-100 ℃ for 30-60 minutes according to the ratio of feed liquid to 1 g:35-45 mL to obtain an pleurotus citrinopileatus Huang Gujin extracting solution, and sequentially carrying out reduced pressure concentration and decolorization treatment on the pleurotus citrinopileatus Huang Gujin extracting solution to obtain the pleurotus citrinopileatus steady state adjustment extracting solution with the concentration of 0.5-2 g/mL; (2) Preparing a ganoderma lucidum steady-state adjustment extracting solution, namely leaching crushed ganoderma lucidum fruit bodies with water at 80-100 ℃ according to the ratio of 1g to 20-30 mL, wherein the leaching time is 3-4 hours, so as to obtain a ganoderma lucidum leaching solution; (3) The preparation method comprises the steps of crushing a hericium erinaceus sample, adding a composite enzymolysis solution into the hericium erinaceus sample according to a feed-liquid ratio of 1g to 25-30mL, and carrying out enzymolysis reaction at a temperature of 35-40 ℃, wherein the composite enzymolysis solution contains an enzymolysis agent with a mass percentage of 0.2-0.3%, the enzymolysis agent consists of cellulase, pectase and papain according to a mass ratio of 1 (1-1.5) (2-3), and is additionally added with an immunoregulatory short peptide HA2 with an addition amount of 0.05g/L or 0.15g/L, after the enzymolysis reaction is finished, heating to carry out water extraction, centrifuging to obtain a supernatant, and obtaining the hericium erinaceus steady-state polysaccharide extract; (4) And (3) compounding a steady-state support agent, namely mixing the pleurotus citrinopileatus steady-state adjustment extracting solution prepared in the step (1), the ganoderma lucidum steady-state adjustment extracting solution prepared in the step (2) and the hericium erinaceus steady-state polysaccharide extracting solution prepared in the step (3) according to the volume ratio of (1:1-1.5): 1 to obtain the immune steady-state support agent.
  2. 2. The immunological steady-state support agent based on the fungus mushroom active ingredient and the fungus polysaccharide according to claim 1, wherein the concentration of the pleurotus citrinopileatus steady-state adjusting extract in the step (1) is 1.0-1.5 g/mL.
  3. 3. The immune homeostasis supporting agent based on mushroom active ingredients and fungus polysaccharide according to claim 1, wherein the concentration of the ganoderma lucidum homeostasis regulating extract in the step (2) is 0.15-0.25 g/mL.
  4. 4. The immunological steady-state supporting agent based on the active ingredients of the mushrooms and the fungus polysaccharide, which is disclosed in claim 1, is characterized in that in the step (3), the enzymolysis reaction time is 2-4 hours, the temperature for water extraction is 90-100 ℃ and the time is 1-2 hours.
  5. 5. The immune homeostasis supporting agent based on fungus mushroom active ingredients and fungus polysaccharide according to claim 1, wherein in the step (4), the mixing volume ratio of the pleurotus citrinopileatus homeostasis regulating extracting solution, the ganoderma lucidum homeostasis regulating extracting solution and the hericium erinaceus homeostasis polysaccharide extracting solution is 1:1:1.
  6. 6. The agent according to claim 1, wherein the active ingredients of the agent act synergistically on the immune system, comprising proteins, polysaccharides, amino acids, ergothioneine, polypeptides, polyphenols and flavonoids, for regulating the immune system to an optimal state of equilibrium.
  7. 7. The agent for supporting immune homeostasis based on active ingredients of mushrooms and fungal polysaccharides according to claim 6, wherein the agent is suitable for patients suffering from disorders of the immune system after treatment by radiotherapy, chemotherapy or the like, and prevents excessive activation of the immune system while providing immune support.
  8. 8. The use of an biostatic support agent based on a fungus mushroom active ingredient and a fungus polysaccharide according to claim 6, wherein the biostatic support agent is used for the preparation of a food, health food or pharmaceutical composition for improving or aiding in the treatment of immune system disorders caused by chemoradiotherapy.

Description

Immune homeostasis supporting agent based on mushroom active ingredients and fungus polysaccharide Technical Field The invention relates to the technical field of biological medicines and functional foods, in particular to an immune homeostasis supporting agent based on mushroom active ingredients and fungus polysaccharide. Background The fruiting body of the mushroom is rich in various bioactive substances such as polysaccharide (such as beta-glucan), triterpene compounds, proteins, polyphenols, ergothioneine, etc. These active ingredients have been widely studied to demonstrate a variety of biological activities, including antioxidant, antitumor and immunomodulatory effects. Among them, the immunoregulation effect is particularly interesting, for example, ganoderan can enhance the nonspecific immune function of the organism by activating macrophages, natural killer cells (NK cells) and the like, while ergothioneine in Pleurotus citrinopileatus is a powerful antioxidant which can help to alleviate the functional impairment of immune cells caused by oxidative stress. However, conventional mushroom extracts or single component preparations tend to focus on unidirectional immunopotentiation, whose mechanism of action may focus on sustained stimulation of immune effector cells. For people with unbalanced immune systems, such as tumor patients undergoing radiotherapy and chemotherapy, the immune system is not simply lowered, but often presents a complex disorder state, and the immune system has the functions and the quantity of immune cells inhibited, and also may have the risk of over-activation of inflammatory factor storm and the like. In this case, if a potent immunostimulant is administered alone, not only may the immune balance not be restored, but there is a risk of inducing or aggravating adverse events such as excessive activation and autoimmune reaction. Aiming at the problems, an immune steady-state supporting agent based on the active ingredients of the mushrooms and the fungus polysaccharide is designed to solve the technical problems. Disclosure of Invention Aiming at the defects of the prior art, the invention provides an immune steady-state supporting agent based on an active component of mushroom and fungus polysaccharide, which solves the technical problems in the background art. The invention aims at realizing the purposes by adopting the following technical scheme that the immunological steady-state supporting agent based on the active ingredients of the mushrooms and the fungus polysaccharide comprises a preparation method, wherein the method comprises the following steps: (1) Preparing a pleurotus citrinopileatus steady state adjustment extracting solution, namely leaching pleurotus citrinopileatus fruiting body powder with water at 80-100 ℃ for 30-60 minutes according to the ratio of feed liquid to 1 g:35-45 mL to obtain an pleurotus citrinopileatus Huang Gujin extracting solution, and sequentially carrying out reduced pressure concentration and decolorization treatment on the pleurotus citrinopileatus Huang Gujin extracting solution to obtain the pleurotus citrinopileatus steady state adjustment extracting solution with the concentration of 0.5-2 g/mL; (2) Preparing a ganoderma lucidum steady-state adjustment extracting solution, namely leaching crushed ganoderma lucidum fruit bodies with water at 80-100 ℃ according to the ratio of 1g to 20-30 mL, wherein the leaching time is 3-4 hours, so as to obtain a ganoderma lucidum leaching solution; (3) The preparation method comprises the steps of crushing a hericium erinaceus sample, adding a composite enzymolysis solution into the hericium erinaceus sample according to a feed-liquid ratio of 1g to 25-30mL, and carrying out enzymolysis reaction at a temperature of 35-40 ℃, wherein the composite enzymolysis solution contains an enzymolysis agent with a mass percentage of 0.2-0.3%, the enzymolysis agent consists of cellulase, pectase and papain according to a mass ratio of 1 (1-1.5) (2-3), and is additionally added with an immunoregulatory short peptide HA2 with an addition amount of 0.05g/L or 0.15g/L, after the enzymolysis reaction is finished, heating to carry out water extraction, centrifuging to obtain a supernatant, and obtaining the hericium erinaceus steady-state polysaccharide extract; (4) And (3) compounding a steady-state support agent, namely mixing the pleurotus citrinopileatus steady-state adjustment extracting solution prepared in the step (1), the ganoderma lucidum steady-state adjustment extracting solution prepared in the step (2) and the hericium erinaceus steady-state polysaccharide extracting solution prepared in the step (3) according to the volume ratio of (1:1-1.5): 1 to obtain the immune steady-state support agent. Preferably, in the step (1), the concentration of the pleurotus citrinopileatus steady state adjusting extract is 1.0-1.5 g/mL. Preferably, in the step (2), the concentration of the ganoderma lucidum steady-sta