CN-121971681-A - Ionic crosslinked network cured composite hemostatic gauze and preparation method thereof

Abstract

The invention discloses an ion crosslinked network solidified composite hemostatic gauze and a preparation method thereof, and relates to the technical field of biomedical materials and biological medicine manufacturing, wherein the preparation method of the ion crosslinked network solidified composite hemostatic gauze comprises the following steps of preparing a base material, and taking chitosan non-woven fabrics as the base material; preparing sizing liquid, padding sizing, immersing a chitosan non-woven fabric substrate in the sizing liquid for padding sizing treatment, pre-drying and shaping, pre-drying the sized chitosan non-woven fabric substrate obtained in the padding sizing step, enhancing in-situ crosslinking, immersing the pre-dried chitosan non-woven fabric substrate obtained in the pre-drying and shaping step in crosslinking liquid containing metal ions for crosslinking reaction, and post-treating. According to the invention, the kaolin particles can be physically embedded on the chitosan non-woven fabric substrate through the ion crosslinking network, so that the kaolin falling rate is reduced, and the use risk of the composite hemostatic gauze is reduced.

Inventors

• RAO YOULIANG
• WANG GANG
• YANG YUPENG
• ZHONG TAO
• ZHOU CHAOMIN
• LUO YONGPENG

Assignees

• 江西如上生物科技有限公司

Dates

Publication Date

20260505

Application Date

20260203

Claims (10)

1. 1. The preparation method of the ionic crosslinked network cured composite hemostatic gauze is characterized by comprising the following steps of: preparing a base material, namely taking chitosan non-woven fabric as the base material; preparing sizing liquid, namely dissolving a water-soluble adhesive in a solvent to prepare a colloid solution, adding kaolin particles into the colloid solution, and uniformly mixing to obtain sizing liquid; Padding and sizing, namely immersing the chitosan non-woven fabric substrate into the sizing liquid for padding and sizing treatment, so that the sizing liquid uniformly permeates and adheres to the fiber surface and the fiber gaps of the chitosan non-woven fabric substrate; pre-drying and shaping, namely, padding the sized chitosan non-woven fabric base material obtained in the sizing step in the following step Pre-drying to reduce the water content of the chitosan non-woven fabric substrate to 20% -50%; in-situ crosslinking reinforcement, immersing the pre-dried chitosan non-woven fabric substrate obtained in the pre-drying and shaping step into crosslinking liquid containing metal ions for crosslinking reaction, and forming a water-insoluble three-dimensional ion crosslinking network by utilizing coordination chelation of the metal ions and water-soluble adhesive molecular chains so as to anchor chitosan fibers in the chitosan non-woven fabric substrate and solidify the kaolin particles; And (3) post-treatment, namely placing the chitosan non-woven fabric substrate obtained in the in-situ crosslinking enhancement step into a cleaning solution to remove redundant metal ions and uncured kaolin, and then carrying out hot air drying or vacuum drying to obtain a target product.
2. 2. The method for preparing an ion crosslinked network cured composite hemostatic gauze according to claim 1, wherein in the step of sizing, the water-soluble binder comprises one or more of carboxymethyl chitosan, sodium alginate, pectin, carboxymethyl cellulose, polygalacturonic acid; And/or, in the step of preparing the sizing agent, the water-soluble binder includes a polymer containing a carboxyl group or a phosphoric acid group.
3. 3. The method for preparing the ionic crosslinked network cured composite hemostatic gauze according to claim 2, wherein the concentration of the water-soluble adhesive is carboxymethyl chitosan Sodium alginate Pectin Carboxymethyl cellulose Hyaluronic acid Polygalacturonic acid And/or polymers containing carboxyl or phosphate groups 。
4. 4. The method for preparing an ion crosslinked network cured composite hemostatic gauze according to claim 1, wherein in the step of sizing, the content of the kaolin particles is 。
5. 5. The method for preparing the ionic crosslinked network cured composite hemostatic gauze according to claim 1, wherein in the step of sizing, the kaolin particles are medical grade kaolin or kaolin, and the particle size of the kaolin particles is smaller than that of the medical grade kaolin or kaolin The particle diameter D50 of the kaolin particles is 。
6. 6. The method for preparing the ion crosslinked network cured composite hemostatic gauze according to claim 1, wherein in the step of preparing the sizing liquid, the sizing liquid is further added with Is glycerol, polyethylene glycol 400, polyethylene glycol 600 or sorbitol.
7. 7. The method for preparing the ionic crosslinked network cured composite hemostatic gauze according to claim 1, wherein the crosslinking liquid containing metal ions in the in-situ crosslinking enhancing step comprises one or more of calcium chloride, calcium lactate, calcium gluconate, calcium dihydrogen phosphate, zinc chloride, zinc lactate, zinc gluconate, zinc citrate and zinc sulfate in ethanol aqueous solution.
8. 8. The method for preparing an ion crosslinked network cured composite hemostatic gauze according to claim 7, wherein the total concentration of metal ions in the crosslinked liquid containing metal ions is The volume fraction of the ethanol is 10% -60%.
9. 9. The method for preparing the ionic crosslinked network cured composite hemostatic gauze according to claim 1, wherein the crosslinking reaction temperature in the in-situ crosslinking enhancing step is as follows The crosslinking reaction time is 1 minute to 30 minutes.
10. 10. An ion crosslinked network cured composite hemostatic gauze, characterized in that the ion crosslinked network cured composite hemostatic gauze is prepared by the preparation method of the ion crosslinked network cured composite hemostatic gauze according to any one of claims 1 to 9, wherein the composite hemostatic gauze comprises a chitosan non-woven fabric substrate, kaolin particles and an ion crosslinked network, the ion crosslinked network is of a water-insoluble three-dimensional network structure, and the kaolin particles are wrapped and confined in a cage form and tightly anchored with chitosan fibers in the chitosan non-woven fabric substrate.

Description

Ionic crosslinked network cured composite hemostatic gauze and preparation method thereof Technical Field The invention relates to the technical field of biomedical material and biological medicine manufacturing, in particular to an ion crosslinked network cured composite hemostatic gauze and a preparation method thereof. Background Blood loss is a leading factor in the death of wounded persons in war wound rescue, traffic accident and surgical hemostasis. And effective hemostasis is of great importance to life saving. At present, mineral hemostatic materials and biopolymer materials are hot spots of research. Kaolin (Kaolin) is a mineral hemostatic material capable of accelerating activation of blood coagulation factor XII, shortening blood coagulation time, promoting local enrichment of blood coagulation factor by utilizing strong adsorptivity, and has been widely applied to hemostatic products. However, the pure kaolin powder has problems such as difficulty in cleaning and possibility of causing inflammatory reaction when in use. Chitosan (Chitosan) is a natural cationic polysaccharide prepared from chitin through deacetylation, is widely applied to the field of medicines, and has good biocompatibility and biodegradability, and auxiliary antibacterial and healing promoting effects. The chitosan has positive charges, can realize hemostasis through charge adsorption and platelet aggregation mechanism, and some chitosan derivatives have high water absorbability, can form gel through rapid water absorption and concentrate blood cells and blood coagulation factors, and improve blood coagulation efficiency. Currently, hemostatic products developed based on chitosan mainly comprise pure chitosan non-woven fabrics, chitosan hemostatic powder, chitosan hemostatic gauze and the like. However, pure chitosan nonwoven fabrics generally do not have strong water absorbability, and hemostasis is achieved mainly by means of charge adsorption and platelet aggregation mechanisms, so that the hemostasis speed is low. Some modified chitosan hemostatic powder has strong water absorbability, obviously accelerated hemostatic speed, but has the problems of uneven spraying and easy scouring and loss by blood flow, and the chitosan powder is compounded on a gauze substrate in a coating mode to prepare chitosan hemostatic gauze, but has the problems of easy falling of chitosan powder, limited chitosan powder loading capacity and the like. In the related art, the Z medical company selects inorganic mineral materials such as kaolin to be loaded on a traditional fabric substrate, and prepares an emergency hemostatic product named QuikClot Combat Gauze. Other physical adhesive-based loading schemes (such as CN106039383A, CN106540305A, etc.) use adhesives such as polyvinyl alcohol (PVA), polyethylene glycol (PEG), or Hypromellose (HPMC) to adhere kaolin and traditional Chinese medicine powder (such as notoginseng powder) to bamboo fiber or chitosan substrates. However, the existing kaolin-based hemostatic products have a large amount of hemostatic material (kaolin) attached to the fabric substrate after contacting blood or water, and the detached particles adhere to wounds and adjacent organs, which is difficult to clean, and may enter into blood vessels to cause the risk of embolism. Disclosure of Invention The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, the invention provides a preparation method of the ionic crosslinked network cured composite hemostatic gauze, which can physically embed kaolin particles on a chitosan non-woven fabric substrate through an ionic crosslinked network, so that the falling rate of the kaolin is reduced, and the use risk of the composite hemostatic gauze is reduced. The invention also provides the ion crosslinked network cured composite hemostatic gauze prepared by the preparation method of the ion crosslinked network cured composite hemostatic gauze. According to an embodiment of the first aspect of the invention, the preparation method of the ionic crosslinked network cured composite hemostatic gauze comprises the following steps: preparing a base material, namely taking chitosan non-woven fabric as the base material; preparing sizing liquid, namely dissolving a water-soluble adhesive in a solvent to prepare a colloid solution, adding kaolin particles into the colloid solution, and uniformly mixing to obtain sizing liquid; Padding and sizing, namely immersing the chitosan non-woven fabric substrate into the sizing liquid for padding and sizing treatment, so that the sizing liquid uniformly permeates and adheres to the fiber surface and the fiber gaps of the chitosan non-woven fabric substrate; pre-drying and shaping, namely, padding the sized chitosan non-woven fabric base material obtained in the sizing step in the following step Pre-drying to reduce the water content of the chitosan non-woven fabric substrate to 20% -50%; In-situ crosslin