CN-121971682-A - Microorganism mineralized spontaneous reactance wound dressing as well as preparation method and application thereof

CN121971682ACN 121971682 ACN121971682 ACN 121971682ACN-121971682-A

Abstract

The invention discloses a microorganism mineralized spontaneous reactor wound dressing, a preparation method and application thereof, comprises an outer breathable protective layer, an outer carbon adhesive conductive layer, a positive and negative separation type paper-based microbial fuel cell assembly and an inner carbon adhesive conductive layer which are sequentially arranged from outside to inside. The invention realizes the three-in-one synergistic function of mineralization restoration, natural antibiosis and spontaneous electric stimulation, solves the key problems that the traditional dressing has single function, the electric stimulation dressing depends on an external power supply, the mechanical strength of hydrogel is insufficient and the like, and the 3D printed micro-grid structure ensures that spores are distributed more uniformly and mineralized more uniformly, has the advantages of excellent biocompatibility, high adhesion, low cost, biodegradability and the like, and is particularly suitable for intelligent care of chronic refractory wounds such as diabetic foot ulcers, pressure ulcers, burns and the like.

Inventors

WANG RUIXING
WANG YAN
SHAO YI

Assignees

东南大学

Dates

Publication Date: 20260505
Application Date: 20260211

Claims (10)

1. A microbial mineralized spontaneous reactance wound dressing is characterized by comprising an outer breathable protective layer, an outer carbon adhesive conducting layer, a forward-reverse separation type paper-based microbial fuel cell assembly and an inner carbon adhesive conducting layer which are sequentially arranged from outside to inside, wherein the forward-reverse separation type paper-based microbial fuel cell assembly comprises cellulose chromatographic paper, a wax isolating layer positioned in the central area of the cellulose chromatographic paper, a bacterial cellulose membrane (BC) positioned on one side of the cellulose chromatographic paper close to a wound and loaded on the inner side of the wax isolating layer to form an anode area, a reducing substance coating positioned on one side of the chromatographic paper far from the wound and positioned on the outer side of the wax isolating layer to form a cathode area, and side carbon adhesive conducting layers positioned on two side surfaces of the cellulose chromatographic paper.
2. The microbial mineralized self-generating antimicrobial wound dressing according to claim 1, wherein the outer breathable protective layer is a medical polyurethane transparent dressing, hydrocolloid transparent dressing, or silicone-based breathable protective dressing.
3. The microbial mineralized self-generating antimicrobial wound dressing according to claim 1, wherein the bacterial cellulose film is formed by 3D printing, has a thickness of 0.3-1.0mm, and has a lattice-like microstructure with 50-100 μm pore size inside.
4. The microbial mineralized self-generating antimicrobial wound dressing according to claim 1, wherein the reducing substance coating is a mixture of manganese dioxide, PEDOT: PSS, DMSO, and deionized water, and the reducing substance coating is formed into a stable reaction layer by 3-5 coating-drying cycles.
5. The microbial mineralized self-generating antimicrobial wound dressing according to claim 1, wherein the MICP antimicrobial functional layer is a bacterial cellulose film loaded with bacillus pasteurizer spores, calcium chloride, urea, graphene oxide, PEDOT: PSS, and DMSO.
6. A microbial mineralization self-generating antimicrobial wound dressing according to claim 5, characterized in that the bacillus pasteurizer is bacillus pasteurizer Sporosarcina pasteurii, preferably ATCC 11859.
7. A method of preparing a microbial mineralized spontaneous bacterial wound dressing according to claim 1, comprising the steps of: (1) Preparation of 3D printed bacterial cellulose film: The preparation method comprises the steps of activating bacillus pasteurizer, inducing sporulation, mixing bacterial cellulose with sodium alginate to prepare 3D printable biological ink, printing the biological ink into a bacterial cellulose film by using a 3D biological printer, immersing the printed bacterial cellulose film (BC) in a mixed solution of PEDOT (poly (styrene-co-butylene) and (butylene) PSS and DMSO, immersing in a mixed solution containing CaCl 2 , urea and graphene oxide, taking out the BC film, spraying bacillus pasteurizer spore suspension on the surface, and air-drying for later use; (2) Assembling the front-back separation type paper-based MFC assembly: the method comprises the steps of taking cellulose chromatographic paper as a substrate, adding wax into the central area of the chromatographic paper to completely melt and permeate the paper, cooling and solidifying to form a wax isolating layer, attaching the 3D printing bacterial cellulose membrane prepared in the step (1) to the inner area of the isolating layer close to one side of a wound, coating a reducing substance coating on the outer area of the isolating layer far from one side of the wound by adopting a coating-drying cycle process, and drawing conductive layers on the edges and the side surfaces of the front side and the back side of the chromatographic paper by using conductive carbon glue: (3) And (3) assembly integration and sterilization: And (3) covering the medical polyurethane transparent dressing with the assembled MFC assembly as an outer breathable protective layer, sterilizing, packaging and refrigerating after sterilizing.
8. The method of claim 7, wherein the drawing the conductive layer on the edges and sides of the front and back sides of the chromatographic paper using the conductive carbon paste in step (2) comprises: (1) Drawing an annular conductive layer with the width of 2-5mm around the edge of the chromatographic paper on one side of the BC membrane; (2) Drawing an annular conductive layer with the width of 2-5mm around the edge of the chromatographic paper on one side of the reducing substance and drawing an X-shaped pattern in the center of the cathode region; (3) The side surfaces are connected with the conductive rings on the front surface and the back surface by carbon glue to form a closed loop.
9. Use of a microbial mineralized self-generating antimicrobial wound dressing according to claim 1 in the preparation of a chronic wound care material or medicament.
10. The use of claim 9, wherein the chronic wounds include diabetic ulcers, pressure ulcers and burn wounds.

Description

Microorganism mineralized spontaneous reactance wound dressing as well as preparation method and application thereof Technical Field The invention belongs to the technical field of medical biological materials, and particularly relates to a microbial mineralized spontaneous reactor wound dressing, and a preparation method and application thereof. Background Wound healing is a complex process involving inflammatory reactions, cell proliferation, tissue remodeling, and especially chronic wounds (such as diabetic foot ulcers and pressure ulcers) are difficult to clinically care due to problems of local microcirculation disturbance, high infection risk, weak repair capacity and the like. The conventional gauze can only passively absorb exudates, has no active repair function, is easy to adhere to wounds and has high infection risk, the hydrogel dressing has good moisture retention but insufficient mechanical strength and limited antibacterial effect, the silver ion dressing can resist bacteria but has heavy metal residue risk, bacterial resistance is easy to cause, the cost is high, and the requirements of basic medical treatment and long-term nursing are difficult to meet. In addition, bioelectric stimulation dressings are becoming a research hotspot for chronic wound care because of their ability to accelerate wound healing by microcurrents. However, the existing bioelectric stimulation dressing has a remarkable technical bottleneck: ① The external power supply is dependent, clinical applicability is poor, most of the electric stimulation dressing needs to be externally connected with a battery or a power adapter to provide electric energy, wearing comfort is reduced due to the fact that the dressing is increased in size and weight, the dressing is easy to be disturbed by activities, electric leakage risks exist, chronic wound surfaces (such as diabetic foot ulcers) of movable parts such as joints and feet are difficult to adapt, few of the passive electric stimulation dressing depends on piezoelectric/triboelectric effects, electricity generation is triggered by external force extrusion, continuous and stable micro-electric stimulation cannot be achieved, and long-term repair requirements of the chronic wound are difficult to meet. ② The traditional product has single function and weak cooperative repair capability, only focuses on the single function of 'electric stimulation', does not integrate key nursing mechanisms such as antibiosis, wound surface sealing and the like, needs to be matched with independent antibiosis dressing or gel for use, increases nursing operation complexity and cost, and has the risks of drug resistance and tissue residue due to the fact that part of the product is added with antibiosis function but depends on heavy metal components such as silver ions and the like. ③ The cost is high, the industrialization difficulty is high, the preparation process of the core component (such as a miniature battery and a precise electrode) is complex, the cost of raw materials is high, the cost bearing range of basic medical treatment and home care is far exceeded, and the large-scale popularization is difficult. Research shows that CaCO 3 particles generated by MICP have good biocompatibility, and calcium ion release can promote cell proliferation, but the application of the particles in the field of wound care is still in an exploration stage, and the existing research only focuses on mineralization repair functions of the particles independently and is not combined with other care mechanisms. Microbial Fuel Cell (MFC) technology converts chemical energy into electrical energy through microbial metabolism, and paper-based MFC has low cost and good flexibility, and exhibits potential in the field of medical sensors. However, the prior art lacks a wound dressing which can integrate the functions of repair, antibiosis and stimulation, has controllable cost and meets clinical requirements. Disclosure of Invention Aiming at the problems existing in the prior art, the invention discloses a microorganism mineralized spontaneous reactance wound dressing, which integrates a microorganism induced Mineralization (MICP) technology and a paper-based biofuel cell (MFC) technology into the wound dressing for the first time, integrates the MICP technology and the paper-based MFC technology for the first time, induces CaCO 3 to form a dynamic mineralization membrane through bacillus pasteurizer (S. pasteurii), can respond to the pH change of wound exudate to realize wound self-adaptive closure, and continuously releases calcium ions to assist tissue regeneration, thereby breaking through the dual-function limitation of the traditional dressing of only 'antibacterial + electric stimulation'. The invention realizes the three-in-one synergistic function of mineralization restoration, natural antibiosis and spontaneous electric stimulation in a breakthrough way, and solves the key problems that the traditional dres