CN-121971687-A - Preparation method and application of canine autologous platelet-rich fibrin membrane

Abstract

The invention provides a preparation method of a canine autologous platelet-rich fibrin membrane, which comprises the steps of collecting canine autologous venous blood by using a vacuum blood collection tube with a preset anticoagulant, centrifuging, discarding supernatant, centrifuging again, discarding lower-layer erythrocytes, placing the obtained canine autologous platelet-rich fibrin gel in a double-sheet stainless steel mesh, and pressing to obtain the canine autologous platelet-rich fibrin membrane. The invention also provides application of the prepared canine autologous platelet-rich fibrin membrane in preparing medicines for wound repair. According to the invention, the autologous platelet-rich fibrin therapy is used for preparing the medicament for repairing the canine wound for the first time, and the triple mechanism of 'autoimmune regulation for replacing antibiotics' + 'time-sequence release of growth factors for promoting healing' + 'zero-allergy protozoon stent' is adopted, so that the common health risk is avoided, and meanwhile, the healing period is shortened, and the medicament is an innovative scheme with safety, effectiveness and sustainability.

Inventors

• SUN HELIN
• SHI YUNWEI
• LONG XIAO
• LI SHUO
• ZHANG XIAN
• ZHANG ZHIAN
• WANG XIN
• LI SUMEI

Assignees

• 石家庄信息工程职业学院

Dates

Publication Date

20260505

Application Date

20260205

Claims (6)

1. 1. A preparation method of a canine autologous platelet-rich fibrin membrane is characterized by comprising the following steps: S1, collecting canine autologous venous blood by using a vacuum blood collection tube with preset anticoagulant, standing vertically, centrifuging, and removing supernatant to obtain centrifuged canine autologous venous blood; S2, performing secondary centrifugation on the centrifuged canine autologous venous blood obtained in the S1, and removing the lower layer red blood cells to obtain canine autologous platelet-rich fibrin gel; And S3, placing the canine autologous platelet-rich fibrin gel obtained in the S2 between double stainless steel grids, and pressing to obtain the canine autologous platelet-rich fibrin membrane.
2. 2. The preparation method of the canine autologous platelet-rich fibrin membrane, which is disclosed in claim 1, is characterized in that the vertical standing time in S1 is 2min, and the centrifugation condition is that the centrifugation is carried out for 5min under the conditions of 20-24 ℃ and 800 rpm.
3. 3. The method for preparing the canine autologous platelet-rich fibrin membrane according to claim 1, wherein the secondary centrifugation condition in S2 is centrifugation for 8min under the conditions that the temperature is 20-24 ℃ and the rotating speed is 2500 rpm.
4. 4. The method for preparing a canine autologous platelet rich fibrin membrane according to claim 1, wherein the pressing condition in S3 is that the pressing is performed for 1min under the pressure of 0.5 MPa.
5. 5. The method for preparing the canine autologous platelet-rich fibrin membrane according to claim 1, wherein the thickness of the canine autologous platelet-rich fibrin membrane in the S3 is 0.8 mm-1.2 mm.
6. 6. Use of a canine autologous platelet rich fiber protein membrane prepared by the method of any one of claims 1-5 for preparing a medicament for wound repair.

Description

Preparation method and application of canine autologous platelet-rich fibrin membrane Technical Field The invention belongs to the technical field of biomedical materials, and particularly relates to a preparation method and application of a canine autologous platelet-rich fibrin membrane. Background The continuous increase of the number of pets and the upgrading of the consumption demands of the pets bring new opportunities and challenges to the field of pet medicine, and along with the continuous expansion of the pet market, the number of canine wound cases caused by various causes is also increased year by year, and under the background of the industry, animal wound treatment is taken as an important component of pet medicine, so that the pet wound treatment has wide development space and innovation demands. The treatment methods commonly used in the field of wound treatment for animals at present comprise surgical repair, biological material transplantation, antibiotic treatment, external traditional Chinese medicine treatment, physical therapy and the like, but the methods have remarkable limitations in healing efficiency, operation burden, scar control and the like. Autologous Platelet Rich Fibrin (PRF) based therapies have not emerged. The pain points of the existing treatment method are as follows: Pain spot 1. Risk of antibiotic abuse and drug resistance. Experience of abuse of broad spectrum antibiotics (such as cephalosporins and fluoroquinolones) is common in veterinary wound treatment, and the root of the phenomenon is lack of necessary bacterial culture and drug sensitivity test for accurate medication. This directly leads to multiple serious consequences, on the one hand, the repeated abuse of antibiotics accelerates the generation and evolution of resistant bacteria (such as pseudomonas aeruginosa) so that the wound surface is difficult to heal, the risk of treatment failure is increased sharply (such as bone nonunion and organ injury), on the other hand, the abuse (such as aminoglycosides and fluoroquinolones) can destroy animal immunity, inhibit vaccine effect and cause double infection, and more seriously, the resistant bacteria and genes thereof can be transmitted to human beings through environment and food chains, thus forming serious public health threat of 'zoonotic drug resistance', and causing huge economic loss and environmental pollution (such as traditional dressing waste containing antibiotics). The pain point 2 is characterized in that the healing period is long and the dressing change is frequently carried out, namely, the wound healing period of the canine soft tissue is long and the dressing change is required frequently, the key period of the ① granulation (3-7 days after the wound) is easy to be influenced by ischemia (such as the distal end of a hindlimb) or drug-resistant bacteria infection (such as pseudomonas aeruginosa), the stagnation is obviously prolonged to more than 14 days, and the healing capacity of the aged or diabetic dogs is further reduced by 40% due to the reduction of the activity of fibroblasts and the delay of collagen deposition. ② The epithelialization process is hindered by the traditional dressing (such as gauze), the new tissue which is easy to adhere and is fragile to mechanically damage during dressing change, and the traditional Chinese medicine (such as myogenic powder) is slow in onset of action (2-3 weeks is needed) and is not suitable for acute infection. The data show that the normal healing of the full-layer defect of the dogs takes 28 days, while the normal healing of the full-layer defect of the dogs takes more than 42 days, and the average chronic wound taking 21 days and changing dressings every day, so that the re-diagnosis rate of the pet hospital is up to 60%, and the treatment time cost, the nursing burden and the animal stress are obviously increased. The pain point 3 is characterized in that part of dogs have obvious allergic conditions to wound healing medicaments, and part of dogs have obvious allergic risks to medicaments in wound treatment, and the core of the pain point is derived from the specificity of canine immune systems and the limitations of traditional medicaments, and the pain point is characterized in that the high genetic risks of specific canine species, namely shepherd dogs (such as Kokyi and side pasturing), carry MDR1 gene mutation, so that the ability of sulfanilamide medicaments to penetrate through blood brain barriers is greatly improved, and the neurotoxicity and allergic incidence rate of the sulfanilamide medicaments are improved by 8 times. Short nasal dogs (e.g., fagacin, bago) are more prone to severe systemic allergic reactions with a 3-fold higher skin mast cell density than other canine species. Dog-specific allergens and sensitization mechanisms are antibiotics (e.g., silver sulfadiazine) which are metabolites that combine with plasma proteins to form haptens that trigger allergies. Dressing ad