CN-121971688-A - Antibacterial composite hydrogel material based on phytic acid chitosan nano particles and pterostilbene and preparation method thereof

Abstract

The invention discloses a bacteriostatic composite hydrogel material based on phytic acid chitosan nano particles and pterostilbene and a preparation method thereof, and belongs to the technical field of biological medicines. The hydrogel composition comprises polyvinyl alcohol, composite nano particles and pterostilbene, wherein the preparation step of the composite nano particles comprises the step of mixing phytic acid and chitosan. The composite hydrogel provided by the invention is superior to single-component PVA hydrogel in various performances. The composite hydrogel disclosed by the invention keeps stable on Young modulus, can absorb water and expand, has a stable network structure and is moderate and stable in degradation rate. The composite hydrogel has an inhibitory effect on both escherichia coli and staphylococcus aureus. In terms of cell compatibility and functionality, the composite hydrogel does not show obvious cytotoxicity in NIH-3T3 fibroblasts and HUVECs endothelial cells, and shows a positive trend of cell viability, adhesion and migration in vitro experiments, and shows improvement compared with pure PVA hydrogel.

Inventors

• Guan Haien
• WU BOJUN
• DONG WENYAN
• ZHANG YUEHAN
• Yuan Ruien
• LIU XUANYU
• CHEN YUEHUA
• HUANG LI

Assignees

• 高州市人民医院

Dates

Publication Date

20260505

Application Date

20260306

Claims (10)

1. 1. A hydrogel composition is characterized by comprising polyvinyl alcohol, composite nano particles and pterostilbene, wherein the preparation step of the composite nano particles comprises the step of mixing phytic acid and chitosan.
2. 2. The hydrogel composition of claim 1, wherein the composite nanoparticles have a particle size of 100 nm to 200 nm.
3. 3. The hydrogel composition of claim 1, wherein the composite nanoparticle has a Zeta potential of +1.0 mV to +2.0 mV in an aqueous system having a pH of 6.5 to 7.2.
4. 4. The hydrogel composition of claim 1, wherein the concentration of pterostilbene in the hydrogel composition is 50 μmol/L.
5. 5. A method of preparing a hydrogel composition comprising the steps of: dissolving chitosan in an aqueous solution of phytic acid, and adjusting the pH value of the obtained system to 6.5-7.2; Mixing the dispersion, polyvinyl alcohol and pterostilbene to obtain a composite precursor solution; the composite precursor solution is subjected to a crosslinking treatment to form the hydrogel composition.
6. 6. The method of preparing a hydrogel composition according to claim 5, wherein the pH is adjusted to 6.8-7.2.
7. 7. The method of preparing a hydrogel composition according to claim 5, wherein the step of crosslinking is performed on the composite precursor solution at least once through a freeze-thaw cycle.
8. 8. The method of preparing a hydrogel composition according to claim 7, wherein the freeze-thaw cycle comprises freezing in an environment of-20 ℃ for 21 hours and thawing at room temperature for 3 hours, and wherein the freeze-thaw cycle is repeated 3 times.
9. 9. Use of the hydrogel composition according to any one of claims 1 to 4 for the preparation of a pharmaceutical composition for inhibiting staphylococcus aureus.
10. 10. Use of the hydrogel composition according to any one of claims 1 to 4 for the preparation of a tissue repair material for promoting proliferation of vascular endothelial cells.

Description

Antibacterial composite hydrogel material based on phytic acid chitosan nano particles and pterostilbene and preparation method thereof Technical Field The invention relates to the technical field of biological medicine, in particular to a bacteriostatic composite hydrogel material based on phytic acid chitosan nano particles and pterostilbene and a preparation method thereof. Background The wound dressing and the anti-infective biological material have important roles in repairing tissue injury, can provide a wet healing environment, block bacterial invasion and promote tissue regeneration. Acute and chronic wounds such as surgical operation, burn, diabetic foot, pressure sore and the like are extremely easy to be infected by various pathogenic microorganisms after being exposed, if the acute and chronic wounds are not effectively protected in time, local inflammation is continuously aggravated, healing delay is caused, and the risk of complications is increased. The hydrogel has the characteristics of high water content, excellent biocompatibility, adjustable mechanical property and the like, and is widely applied to wound dressing, soft tissue substitution materials and drug delivery systems. However, hydrogels constructed by traditional natural or synthetic polymer monomer systems generally have the problems of insufficient mechanical strength, limited antibacterial performance, lack of biological activity and the like, and are difficult to meet the comprehensive treatment requirements of complex wound surfaces. To improve the overall properties of hydrogels, researchers often use natural polysaccharides to crosslink with organic acids containing polyphosphoric acid groups to form more stable composite structures. The polyphosphoric acid groups can provide a higher density of negative charges, thereby enhancing electrostatic and hydrogen bond interactions with the polysaccharide chains and improving the structural stability and bacteriostatic ability of the nanocomposite system. However, the prior art still has the problems of insufficient nanoparticle dispersibility, poor stability, limited inhibition effect on partial drug-resistant strains and the like, and influences the application of the nano-particles in complex wound environments. On the other hand, part of natural small molecular active substances have the functions of bacteriostasis, antioxidation and inflammation regulation, and can be used for improving the bioactivity of the hydrogel. However, the active substances generally have the defects of poor chemical stability, easy degradation in the hydrogel, difficult precise control of release behavior and the like, and the mechanical property and broad-spectrum antibacterial capability of the hydrogel cannot be considered when the active substances are singly doped. In conclusion, the existing hydrogel and anti-infective materials still have obvious limitations in terms of mechanical strength, structural stability, biological activity and broad-spectrum antibacterial capability, and the comprehensive requirements of clinic on the wound dressing with mechanical stability, biocompatibility and synergistic anti-infective function are difficult to meet. Therefore, it is necessary to construct a novel composite hydrogel material with good mechanical property, structural stability, bioactivity and antibacterial capacity so as to better meet the application requirements of clinical wound surface management. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a bacteriostatic composite hydrogel material based on phytic acid chitosan nano particles and pterostilbene and a preparation method thereof. PVA is used for constructing a three-dimensional network structure of hydrogel, PVA aqueous solution with a certain mass fraction can be used as a gel forming matrix, PA and CS form a nano composite structure through electrostatic interaction and hydrogen bonding, the structure can cooperate with the PVA network to construct a stable composite gel system, PTE can be loaded in the gel system through physical adsorption, complexation or other non-covalent interaction modes with the composite network, and the dosage of PTE can be adjusted according to target performances. The hydrogel of the invention can be prepared by mixing PVA solution with a preformed PA/CS composite nano structure, uniformly dispersing the PVA solution in a matrix, then introducing pterostilbene, fully stirring the pterostilbene, loading the pterostilbene in a gel network, and obtaining the composite hydrogel material through proper freeze-thawing cycle, chemical crosslinking or other gel forming modes. The regulation and control of the mechanical property, biocompatibility and antibacterial property of the hydrogel can be realized by regulating the PVA concentration, the proportion of the PA and the CS and the addition amount of the PTE. The aim of the invention can be achieved by the following technica