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CN-121971691-A - PH intelligent response Se quantum dot polysaccharide hydrogel, preparation method and medical application thereof

CN121971691ACN 121971691 ACN121971691 ACN 121971691ACN-121971691-A

Abstract

The invention belongs to the technical fields of biomedical materials and biomedical engineering, and discloses a pH intelligent response Se quantum dot polysaccharide hydrogel, a preparation method and medical application thereof. The preparation method comprises the steps of modifying gelatin with cyclodextrin to obtain cyclodextrin gelatin, modifying peach gum with amantadine to obtain amantadine peach gum, mixing the cyclodextrin gelatin, the amantadine peach gum and selenium quantum dots, and obtaining the pH intelligent response Se quantum dot polysaccharide hydrogel based on interaction of a host and a guest.

Inventors

  • CHEN SHI
  • ZHU SHURUI
  • SHEN LIUCHANG
  • ZHANG ZHENZHU
  • MA YUHAN
  • XUAN HONGYUN
  • YUAN HUIHUA

Assignees

  • 南通大学

Dates

Publication Date
20260505
Application Date
20260318

Claims (10)

  1. 1. The preparation method of the pH intelligent response Se quantum dot polysaccharide hydrogel is characterized by comprising the following steps of: S1, using 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide to activate carboxymethyl-beta-cyclodextrin sodium salt, then mixing the obtained activated product with gelatin solution for reaction, and dialyzing and freeze-drying after the reaction is completed to obtain cyclodextrin gelatin; S2, dissolving peach gum in water, adding 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide for activation treatment, then adding amantadine into a reaction system for reaction, dialyzing the obtained reaction solution after the reaction is finished, and freeze-drying to obtain amantadine peach gum; S3, adding cyclodextrin gelatin and amantadine peach gum into the water in which the selenium quantum dots are dispersed, stirring and standing to obtain the pH intelligent response Se quantum dot polysaccharide hydrogel.
  2. 2. The preparation method according to claim 1, wherein in the step S1, the mass ratio of gelatin, carboxymethyl-beta-cyclodextrin sodium salt, 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide is 1000 (2200-2500): 1800-1900): 1000-1200.
  3. 3. The preparation method according to claim 2, wherein in the step S1, the activation time is 15 to 30 minutes and the reaction time is 8 to 12 hours.
  4. 4. The preparation method of claim 1, wherein in the step S2, the mass ratio of peach gum, amantadine, 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide is 1000 (500-600): 900-950): 500-600.
  5. 5. The method according to claim 4, wherein in the step S2, the activation treatment is performed for 15 to 30 minutes and the reaction time is 8 to 12 hours.
  6. 6. The method of claim 1, wherein the dialysis is for a period of 3 to 5 days.
  7. 7. The preparation method of claim 1, wherein in the step S3, the feeding ratio of cyclodextrin gelatin, amantadine peach gum, selenium quantum dots and water is (120-140) mg (40-50) mg (6-12) mg/1000 mL.
  8. 8. A pH intelligent response Se quantum dot polysaccharide hydrogel prepared according to the preparation method of any one of claims 1-7.
  9. 9. The use of the pH intelligent response Se quantum dot polysaccharide hydrogel according to claim 8 for preparing a material for skin injury pH monitoring or skin injury repair.
  10. 10. The pH intelligent response material is characterized by being obtained by dehydrating and drying the pH intelligent response Se quantum dot polysaccharide hydrogel in accordance with claim 8.

Description

PH intelligent response Se quantum dot polysaccharide hydrogel, preparation method and medical application thereof Technical Field The invention belongs to the technical field of biomedical materials, and relates to a pH intelligent response Se quantum dot polysaccharide hydrogel, a preparation method and medical application thereof. Background Diabetes is a chronic metabolic disease characterized by hyperglycemia, microangiopathy-induced tissue hypoxia, oxidative stress, and persistent inflammatory response, often resulting in metabolic disorders in the body. Diabetic wounds (e.g., diabetic foot ulcers) are a common complication. For healthy organisms, wound healing is an orderly process involving four stages of hemostasis, inflammation, proliferation and remodeling, but many pathological factors present in diabetics interrupt this process. Traditional dressing can be clinically used for physical isolation of conventional acute wound, but for chronic difficult-to-heal wound caused by diabetes, the traditional dressing cannot maintain a moist environment favorable for healing, and adhesion with newly-grown granulation tissues is extremely easy to occur when the dressing is replaced, so that secondary injury is caused, and infection risk is increased. In addition, the healing management of chronic wounds also faces a number of problems, such as increased bacterial resistance, difficult real-time monitoring of the wound microenvironment (pH value), and the like. In recent years, scientific researchers are actively exploring and developing novel medical biological glue and functional hydrogel dressing aiming at the defects of the traditional dressing. The bioactive material is used to replace traditional dry dressing to realize wet wound healing, and its excellent adhesion and self-repairing performance are used in clinical treatment to fill irregular wound to optimize regenerated micro environment and reduce wound infection rate. In addition, a single treatment mode is difficult to feed back wound healing state in real time, such as dynamic change of pH value is directly related to bacterial infection and angiogenesis, and the lack of real-time monitoring of the key index often delays the optimal treatment time. However, most commercial products on the market are chemical synthetic materials, the problems of poor biocompatibility, bacteria breeding gaps caused by inaccurate fitting of complex wound surfaces and the like are still not negligible, and the feedback capability on wound healing pH change is lacking, so that the further application of the commercial products in accurate medical treatment is limited. Therefore, the composite hydrogel integrating good biocompatibility, complex wound surface fitting tightness and pH visual monitoring is developed, and has important clinical significance for breaking through the bottleneck of diabetes wound surface treatment. Disclosure of Invention In view of the above, the invention aims to provide a pH intelligent response Se quantum dot polysaccharide hydrogel, a preparation method and medical application thereof, wherein the hydrogel has excellent self-repairing performance and good biocompatibility, has a pH monitoring function, and can be applied to monitoring and treating skin injury pH of diabetes wounds and the like. The invention provides a preparation method of a pH intelligent response Se quantum dot polysaccharide hydrogel, which is characterized by comprising the following steps of: S1, using 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide to activate carboxymethyl-beta-cyclodextrin sodium salt, then mixing the obtained activated product with gelatin solution for reaction, and dialyzing and freeze-drying after the reaction is completed to obtain cyclodextrin gelatin; S2, dissolving peach gum in water, adding 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide for activation treatment, then adding amantadine into a reaction system for reaction, dialyzing the obtained reaction solution after the reaction is finished, and freeze-drying to obtain amantadine peach gum; S3, adding cyclodextrin gelatin and amantadine peach gum into the water in which the selenium quantum dots are dispersed, stirring and standing to obtain the pH intelligent response Se quantum dot polysaccharide hydrogel. In some embodiments of the invention, in step S1, the mass ratio of gelatin, carboxymethyl-beta-cyclodextrin sodium salt, 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide is 1000 (2200-2500): 1800-1900): 1000-1200. In some embodiments of the present invention, in step S1, the activation time is 15 to 30 minutes, and the reaction time is 8 to 12 hours. In some embodiments of the present invention, in step S2, the mass ratio of peach gum, amantadine, 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride, and N-hydroxysuccinimide is 1000 (500-600): 900-950): 500-600. In