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CN-121971693-A - Functional rare earth-containing growth factor gel and preparation method thereof

CN121971693ACN 121971693 ACN121971693 ACN 121971693ACN-121971693-A

Abstract

The invention provides a functional rare earth-containing growth factor gel and a preparation method thereof, wherein the gel is prepared from a matrix, NGF and rare earth-manganese bimetallic nano enzyme CeMn NP-PEG with the mass ratio of 10:1 (0.2-1), wherein the CeMn NP-PEG is CeMn NP with PEG modified surface, the CeMn NP is prepared from Ce (Ac) 3 ·nH 2 O and Mn (Ac) 3 ·4H 2 O through sol-gel reaction, and the doping amount of Ce is 20-50 wt%. According to the gel, ceMn NP-PEG, NGF and a matrix are integrated into an integrated functional dressing, and an anti-oxidation function is exerted through the ROS scavenging effect, so that a foundation is laid for realizing the effects of inflammatory regulation, nerve-blood vessel-epidermis cooperative repair and healing promotion in the follow-up process, and the clinical requirements of rapid and high-quality healing of common wounds are hopefully met.

Inventors

  • LI LU
  • Qiao Pengju
  • ZHANG CHUNXIA
  • LIU GANG
  • Sun Pizhi
  • CUI HONGLIANG
  • GUO PENGBO
  • KAN LIXIN

Assignees

  • 天津包钢稀土研究院有限责任公司

Dates

Publication Date
20260505
Application Date
20260409

Claims (10)

  1. 1. A functional gel containing rare earth growth factors is characterized by being prepared from a matrix, NGF and rare earth-manganese bimetallic nano enzyme CeMn NP-PEG in a mass ratio of 10:1 (0.2-1), wherein the CeMn NP-PEG is a CeMn NP with PEG modified on the surface, the CeMn NP is prepared from Ce (Ac) 3 ·nH 2 O and Mn (Ac) 3 ·4H 2 O through a sol-gel reaction, and the doping amount of the Ce is 20-50 wt%.
  2. 2. The functional rare earth-containing growth factor gel according to claim 1, wherein the CeMn NP is prepared by adding Ce (Ac) 3 ·nH 2 O and Mn (Ac) 3 ·4H 2 O to a mixed solution of polyimide and xylene at 100 ℃ through a sol-gel reaction, and the feed liquid ratio of polyimide to xylene is M: V=1 (5-10).
  3. 3. The method for preparing a functional rare earth-containing growth factor gel according to claim 1, wherein the method comprises mixing a chloroform solution of CeMn NP with DSPE-PEG 2K and stirring at room temperature to obtain a mixture, evaporating the mixture by a rotary evaporator, adding distilled water into the dried mixture to redisperse, and filtering with an ultrafiltration tube to obtain the CeMn NP-PEG.
  4. 4. The functional rare earth-containing growth factor gel according to claim 3, wherein the concentration of the chloroform solution of CeMn NP is 1-3mg/mL, and the weight portion of DSPE-PEG 2K is 8-12; stirring for 30-60 minutes at room temperature; The temperature of rotary evaporation is 50-70 ℃ and the time is 2-5 hours; the mass ratio of the distilled water to the dry mixture is (3-5): 1; the molecular weight cut-off value of the ultrafiltration tube is 30000-40000.
  5. 5. The functional rare earth-containing growth factor gel of claim 1, wherein CeMn NP-PEG is approximately spherical and has a size of 2.0-15.0nm.
  6. 6. The rare earth-containing growth factor gel of claim 1, wherein the matrix is PEGDA.
  7. 7. A method for preparing a functional rare earth-containing growth factor gel according to any one of claims 1to 6, comprising the steps of: 1. Adding PBS particles into a buffer bottle, and adding water into the buffer bottle to heat in a water bath at 40-50 ℃ so as to promote the PBS particles to dissolve, thereby obtaining PBS buffer solution; 2. Adding the matrix into the PBS buffer solution for dissolution; 3. Preparing NGF and CeMn NP-PEG into solutions respectively, then adding the NGF solution and the CeMn NP-PEG solution into the solution dissolved in the second step according to a proportion, and uniformly mixing; 4. the mixed solution is exposed to ultraviolet light to initiate gelation, resulting in the gel.
  8. 8. The method of claim 7, wherein in the first step, the concentration of PBS buffer solution is 0.01M, and the gel is heated in a water bath for 15-30min.
  9. 9. The method of claim 7, wherein in the second step, the solution ratio M of the matrix to PBS buffer solution is V=1:10, and the solution is dissolved at room temperature for 10-20min.
  10. 10. The method for preparing a functional rare earth-containing growth factor gel according to claim 7, wherein in the third step, the concentration of NGF solution is 1000-1500 μg/mL, and the concentration of CeMn NP-PEG solution is 200-500 μg/mL; in the fourth step, the reaction is carried out for 40 to 60 seconds under ultraviolet light.

Description

Functional rare earth-containing growth factor gel and preparation method thereof Technical Field The invention belongs to the field of medical materials, and particularly relates to a functional rare earth-containing growth factor gel and a preparation method thereof. Background Clinically common acute and chronic wounds (such as surgical incisions, burns, diabetic foot ulcers, pressure sores, etc.) often have delayed healing due to ischemia, infection, and persistent inflammatory reactions. The excessive inflammation stage of the wound surface is characterized in that neutrophils and macrophages are infiltrated in a large amount, proinflammatory factors such as IL-1 beta, IL-6, TNF-alpha and the like are released, and along with the burst of Reactive Oxygen Species (ROS), extracellular matrix degradation, fibroblast apoptosis and capillary vessel injury are caused, so that the chronic wound surface difficult to heal is finally formed. Nerve Growth Factor (NGF), an important bioactive molecule, has been demonstrated to accelerate repair of skin wounds by promoting keratinocyte migration, collagen deposition and angiogenesis. NGF plays a key role in the wound healing process, and can promote proliferation, differentiation and migration of cells by activating various cell signaling pathways, thereby accelerating the wound healing process. However, NGF has low bioavailability in wound surface, is easily diluted by exudates or degraded by protease, and is difficult to maintain effective concentration at wound surface. In addition, NGF has a short half-life and is often metabolized rapidly in vivo, further limiting its use in wound repair. In recent years, nanoezymes have been widely studied for scavenging ROS due to their unique catalytic properties. Cerium oxide (CeO 2) nano particles show broad-spectrum ROS scavenging capacity by virtue of Ce 3+/Ce4+ reversible redox cycle, and manganese oxide (MnO x) efficiently catalyzes H 2O2 decomposition through Mn 2+/Mn3+/Mn4+ multi-valence state change. However, single nano-enzyme has the defects of limited catalytic efficiency, easy agglomeration, poor compatibility with biological macromolecules and the like. Hydrogels are widely used as wound dressings due to their high water content, soft texture and injectability. However, conventional hydrogels only provide physical covering and moisturizing functions, lack active anti-inflammatory, antioxidant and repair promoting capabilities, and have difficulty in achieving uniform loading and simultaneous release of hydrophilic growth factors and hydrophobic inorganic nanomaterials. Disclosure of Invention In view of the above, the invention aims to provide a functional rare earth-containing growth factor gel and a preparation method thereof, which integrate rare earth-manganese bimetallic nano enzyme CeMn NP-PEG, NGF and a matrix into an integrated functional dressing, play an antioxidant role through the ROS scavenging effect, lay a foundation for realizing the effects of inflammatory regulation, nerve-blood vessel-epidermis cooperative repair and healing promotion subsequently, and are expected to meet the clinical requirements of rapid and high-quality healing of common wounds. In order to achieve the above purpose, the technical scheme of the invention is realized as follows: A functional gel containing rare earth growth factors is prepared from a matrix, NGF (nerve growth factor) and rare earth-manganese bimetallic nano enzyme CeMn NP-PEG in a mass ratio of 10:1 (0.2-1), wherein the CeMn NP-PEG is a CeMn NP with PEG modified surface, the CeMn NP is prepared from Ce (Ac) 3·nH2 O and Mn (Ac) 3·4H2 O through a sol-gel reaction, and the doping amount of the Ce is 20-50 wt%. Further, the preparation method of the CeMn NP comprises the steps of adding cerium acetate Ce (Ac) 3·nH2 O and manganese acetate Mn (Ac) 3·4H2 O into a mixed solution of polyimide and dimethylbenzene, and carrying out sol-gel reaction at 100 ℃ to obtain the CeMn NP, wherein the feed liquid ratio M of the polyimide and the dimethylbenzene is V=1 (5-10) (g/ml). Further, ceMn NP-PEG was prepared by mixing a chloroform solution of CeMn NP with DSPE-PEG 2K and stirring at room temperature to obtain a mixture, then evaporating the mixture with a rotary evaporator, then adding distilled water to the dried mixture to redisperse and filtering with an ultrafiltration tube, thereby obtaining CeMn NP-PEG. Further, according to the mass parts, DSPE-PEG 2K is 8-12 parts, the chloroform solution of CeMn NP is 1-3 parts, and the concentration of the chloroform solution of CeMn NP is 1-3mg/mL; stirring for 30-60 minutes at room temperature; The temperature of rotary evaporation is 50-70 ℃ and the time is 2-5 hours; the mass ratio of the distilled water to the dry mixture is (3-5): 1; the molecular weight cut-off value of the ultrafiltration tube is 30000-40000. Further, ceMn NP-PEG was nearly spherical with a size of 2.0-15.0nm. Further, the matrix is PEGDA. The matri