CN-121971695-A - Bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration and preparation method thereof

Abstract

The invention provides a bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration and a preparation method thereof, and relates to the technical field of biomedical materials, wherein the bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration comprises a porous magnesium alloy bracket, and bionic hydrogel HAMA-RGD@BMM-KGN and HAMA-RGD@BMM-Q which are solidified in pores of the porous magnesium alloy bracket in a layered manner; the HAMA-RGD@BMM-KGN is a bionic hydrogel formed by dispersing the BMM loaded with KGN in a HAMA-RGD solution, and the HAMA-RGD@BMM-Q is a bionic hydrogel formed by dispersing the BMM loaded with quercetin Q in the HAMA-RGD solution. The bionic magnesium alloy material for inducing the synchronous regeneration of the bone and the cartilage can provide stable three-dimensional mechanical support on the macroscopic scale, rebuild the space gradient structure of the cartilage and the subchondral bone on the microscopic scale, and realize the synchronous and partitioned regeneration of the bone and the cartilage.

Inventors

• WANG HONG
• PENG YIZHONG
• LI DONGXU
• MENG CHUNQING
• HUANG WEI
• SUN TINGFANG

Assignees

• 华中科技大学同济医学院附属协和医院

Dates

Publication Date

20260505

Application Date

20260403

Claims (10)

1. 1. A bionic magnesium alloy material for inducing synchronous regeneration of bone and cartilage is characterized by comprising a porous magnesium alloy bracket, and a bionic hydrogel HAMA-RGD@BMM-KGN and a bionic hydrogel HAMA-RGD@BMM-Q which are solidified in pores of the porous magnesium alloy bracket in a layered manner, wherein the HAMA-RGD@BMM-KGN is the bionic hydrogel formed by dispersing a stem cell membrane vesicle BMM loaded with KGN in a HAMA-RGD solution, and the HAMA-RGD@BMM-Q is the bionic hydrogel formed by dispersing the BMM loaded with quercetin Q in the HAMA-RGD solution.
2. 2. The bionic magnesium alloy material for inducing synchronous bone-cartilage regeneration according to claim 1, wherein the HAMA-rgd@bmm-KGN is solidified at 1/3 of the upper layer of the porous magnesium alloy scaffold, and the HAMA-rgd@bmm-Q is solidified at 2/3 of the lower layer of the porous magnesium alloy scaffold.
3. 3. The biomimetic magnesium alloy material for inducing synchronous bone-cartilage regeneration of claim 1, wherein the HAMA-RGD solution comprises 2% HAMA, 1% RGD, and 0.1% photoinitiator in terms of mass-to-volume ratio.
4. 4. The biomimetic magnesium alloy material for inducing synchronous bone-cartilage regeneration according to claim 1, wherein the porous magnesium alloy scaffold is a three-dimensional scaffold with a pore size of 500 μm.
5. 5. The biomimetic magnesium alloy material for inducing synchronous bone-cartilage regeneration according to claim 1, wherein the porous magnesium alloy scaffold is made of magnesium-zinc alloy, wherein the magnesium-zinc alloy comprises 95-99% magnesium and 1-5% zinc by mass fraction.
6. 6. The method for preparing a bionic magnesium alloy material inducing synchronous bone-cartilage regeneration according to any one of claims 1 to 5, comprising the steps of: Dispersing the BMM loaded with KGN in HAMA-RGD solution to form bionic hydrogel HAMA-RGD@BMM-KGN; dispersing the BMM loaded with Q in HAMA-RGD solution to form bionic hydrogel HAMA-RGD@BMM-Q; and layering and pouring the HAMA-RGD@BMM-KGN and the HAMA-RGD@BMM-Q into pores of a porous magnesium alloy bracket, and curing to obtain the bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration.
7. 7. The preparation method of the BMM loaded with KGN, which is characterized in that the preparation method comprises the steps of preparing KGN into a mother solution of 10-20mg/mL, mixing KGN and BMM according to a mass ratio of 1:50, and incubating for 1h at 37 ℃ to obtain the BMM-KGN.
8. 8. The preparation method of the BMM loaded with Q according to claim 6, wherein the preparation method comprises the steps of preparing Q into a Q solution of 10-20mg/mL, mixing the Q solution with BMM according to the mass ratio of Q to BMM of 1:50, and incubating at 37 ℃ for 1h to obtain BMM-Q.
9. 9. The preparation method of the bionic magnesium alloy material for inducing the bone-cartilage synchronous regeneration according to the preparation method of the porous magnesium alloy support, which is characterized in that the HAMA-RGD@BMM-KGN and the HAMA-RGD@BMM-Q are layered and poured into the pores of the porous magnesium alloy support, and the specific steps of curing to obtain the bionic magnesium alloy material for inducing the bone-cartilage synchronous regeneration comprise the steps of pouring the HAMA-RGD@BMM-KGN into the upper layer 1/3 of the porous magnesium alloy support and photo-curing, and then pouring the HAMA-RGD@BMM-Q into the lower layer 2/3 of the porous magnesium alloy support and photo-curing to obtain the bionic magnesium alloy material for inducing the bone-cartilage synchronous regeneration.
10. 10. The method according to claim 9, wherein the photo-curing is carried out under a light having a wavelength of 405nm for 30 to 60 seconds.

Description

Bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration and preparation method thereof Technical Field The invention relates to the technical field of biomedical materials, in particular to a bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration and a preparation method thereof. Background The bone-cartilage unit is a functional core for maintaining the weight bearing, buffering and stable movement of the joint, and the microstructure of the bone-cartilage unit is hyaline cartilage, calcified cartilage and subchondral bone in sequence from the joint surface downwards, and the bone-cartilage unit presents obvious composition and mechanical gradient. Once a large area osteochondral defect occurs throughout the cartilage and subchondral bone, localized imbalance in stress distribution, sustained degeneration of the cartilage matrix, eventually may progress to severe pain, restricted activity and even end-stage joint disease. Such large scale osteochondral defects are common in the progression of trauma, sports injury and osteoarthritis, but currently there is no ideal therapeutic means for simultaneously reconstructing the complete structure and function of cartilage and subchondral bone. Clinically existing repair strategies, such as microfracture, autologous/allogeneic cartilage transplantation, autologous chondrocyte transplantation and the like, can recover the continuity of the joint surface to a certain extent in small-range focal defects, but generally face the problems of insufficient donor, poor geometric and mechanical matching of the graft and a receiving area, poor cartilage-bone interface integration, multiple fibrocartilage-like repair tissues and the like. These defects make it difficult for the repair tissue to withstand the complex joint loading environment for a long period of time, and collapse, re-injury or disease recurrence are likely to occur. Therefore, how to macroscopically provide stable three-dimensional mechanical support and microscopically reconstruct the spatial gradient structure of cartilage and subchondral bone, so as to realize synchronous and partitioned regeneration of 'bone-cartilage', and the method is a problem to be solved in the technical field of current bone-cartilage repair. Disclosure of Invention Aiming at the defects in the prior art, the invention solves the technical problem of providing a bionic magnesium alloy material for inducing the synchronous regeneration of bone and cartilage and a preparation method thereof, which can macroscopically provide stable three-dimensional mechanical support, microscopically reconstruct the spatial gradient structure of cartilage and subchondral bone and realize the synchronous and partitioned regeneration of the bone and cartilage. In order to achieve the above object, in a first aspect, the invention provides a bionic magnesium alloy material for inducing synchronous regeneration of bone and cartilage, which is characterized by comprising a porous magnesium alloy bracket, and bionic hydrogels HAMA-RGD@BMM-KGN and HAMA-RGD@BMM-Q which are solidified in the pores of the porous magnesium alloy bracket in a layering manner, wherein the HAMA-RGD@BMM-KGN is the bionic hydrogel formed by dispersing BMM loaded with KGN in a HAMA-RGD solution, and the HAMA-RGD@BMM-Q is the bionic hydrogel formed by dispersing BMM loaded with quercetin Q in a HAMA-RGD solution. Preferably, the HAMA-RGD@BMM-KGN is solidified on the upper layer 1/3 of the porous magnesium alloy bracket, and the HAMA-RGD@BMM-Q is solidified on the lower layer 2/3 of the porous magnesium alloy bracket. Preferably, the HAMA-RGD solution comprises 2% HAMA, 1% RGD, and 0.1% photoinitiator in terms of mass to volume ratio. Preferably, the porous magnesium alloy scaffold is a three-dimensional scaffold with a pore size of 500 μm. Preferably, the porous magnesium alloy bracket is made of magnesium-zinc alloy, wherein the magnesium-zinc alloy comprises 95-99% of magnesium and 1-5% of zinc by mass fraction. In a second aspect, the present invention also provides a preparation method of a bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration, the preparation method comprising the steps of: Dispersing the BMM loaded with KGN in HAMA-RGD solution to form bionic hydrogel HAMA-RGD@BMM-KGN; dispersing the BMM loaded with Q in HAMA-RGD solution to form bionic hydrogel HAMA-RGD@BMM-Q; and layering and pouring the HAMA-RGD@BMM-KGN and the HAMA-RGD@BMM-Q into pores of a porous magnesium alloy bracket, and curing to obtain the bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration. Preferably, the preparation method of the BMM loaded with KGN comprises the steps of preparing KGN into a mother solution of 10-20mg/mL, mixing KGN and BMM according to a mass ratio of 1:50, and incubating at 37 ℃ for 1h to obtain the BMM-KGN. Preferably, the preparation method of the BMM loa