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CN-121971706-A - Hydrogels containing 7-amino acid phosphorylated peptides and their use in nerve repair

CN121971706ACN 121971706 ACN121971706 ACN 121971706ACN-121971706-A

Abstract

The application discloses a hydrogel containing 7-amino acid phosphorylated peptide and application thereof in nerve repair, wherein the hydrogel comprises acellular matrix hydrogel and 7-amino acid phosphorylated peptide loaded in the acellular matrix hydrogel. The 7-amino acid phosphorylated peptide can play a multi-dimensional regulation and control role in nerve injury microenvironment remodeling, realize dynamic targeted regulation and control on peripheral nerve injury microenvironment, and promote rapid regeneration and recovery of injured peripheral nerves.

Inventors

  • SUN WENZHI
  • Ran Mingzi
  • LI YAN

Assignees

  • 首都医科大学
  • 中国人民解放军总医院第四医学中心

Dates

Publication Date
20260505
Application Date
20251217

Claims (10)

  1. 1. A hydrogel for repairing a neurological deficit, the hydrogel comprising: Acellular matrix hydrogels, and 7-Amino acid phosphorylated peptides loaded in the acellular matrix hydrogel.
  2. 2. The hydrogel of claim 1, wherein the concentration of the 7-amino acid phosphorylated peptide in the hydrogel for repairing a neurological deficit is 30 μg/ml to 70 μg/ml based on the total mass of the hydrogel for repairing a neurological deficit.
  3. 3. The hydrogel of claim 1, wherein the 7-amino acid phosphorylated peptide repairs neurological defects by at least one of the following mechanisms: (1) Promoting polarization of macrophages to the anti-inflammatory phenotype M2; (2) Promoting vascular matrix network remodeling, wherein the promoting vascular matrix network remodeling comprises at least one of enhancing proliferative activity of vascular endothelial cells and promoting migration of vascular endothelial cells; (3) Enhancing the function of the schwann cells, wherein the enhancing the function of the schwann cells comprises improving at least one of proliferation capacity, migration capacity and secretory function of the schwann cells.
  4. 4. The hydrogel of any one of claims 1 to 3, wherein the 7-amino acid phosphorylated peptide is supported in the acellular matrix hydrogel by at least one of covalent bonding, non-covalent bonding, and physical entrapment.
  5. 5. The hydrogel of any one of claims 1 to 3, wherein the acellular matrix hydrogel comprises acellular matrix, the acellular matrix having a concentration of 0.3% (w/v) to 0.9% (w/v) in the acellular matrix hydrogel.
  6. 6. Use of a 7-amino acid phosphorylated peptide in the preparation of a product for repairing a neurological deficit.
  7. 7. Use according to claim 6, characterized in that the product comprises at least one of the following products: (1) A hydrogel; (2) A three-dimensional scaffold; (3) A kit.
  8. 8. The use according to any one of claims 6 to 7, wherein the 7-amino acid phosphorylated peptide repairs a neurological deficit by at least one of the following mechanisms: (1) Promoting polarization of macrophages to the anti-inflammatory phenotype M2; (2) Promoting vascular matrix network remodeling, wherein the promoting vascular matrix network remodeling comprises at least one of enhancing proliferative activity of vascular endothelial cells and promoting migration of vascular endothelial cells; (3) Enhancing the function of the schwann cells, wherein the enhancing the function of the schwann cells comprises improving at least one of proliferation capacity, migration capacity and secretory function of the schwann cells.
  9. 9. The use according to any one of claims 6-7, wherein the neurological deficit comprises at least one of a spinal nerve injury, a cranial nerve injury, and an autonomic nerve injury.
  10. 10. The use according to any one of claims 6 to 7, wherein the therapeutically effective amount of the 7-amino acid phosphorylated peptide is 0.002 mg/kg body weight to 0.008 mg/kg body weight.

Description

Hydrogels containing 7-amino acid phosphorylated peptides and their use in nerve repair Technical Field The application belongs to the technical field of tissue engineering and regenerative medicine, and particularly relates to a hydrogel containing 7-amino acid phosphorylated peptide and application thereof in nerve repair. Background Peripheral Nerve Injury (PNI) refers to the destruction of peripheral nerve structure or function caused by trauma (e.g., cutting, squeezing), disease (e.g., diabetes, autoimmune disease) or iatrogenic factors (e.g., surgical injury), thereby causing sensory, motor and autonomic nerve dysfunction. Traditional single-function treatment strategies are generally difficult to quickly adapt to unbalanced damaged microenvironments, and cannot realize dynamic targeted regulation and control on peripheral nerve damaged microenvironments, so that nerve regeneration is slow and functional recovery is limited. Disclosure of Invention The embodiment of the application provides a hydrogel containing 7-amino acid phosphorylating peptide and application thereof in nerve repair, which can realize regeneration and recovery of injured peripheral nerves. In a first aspect, the present application provides a hydrogel for repairing a neurological deficit, the hydrogel comprising a decellularized matrix hydrogel, and a 7-amino acid phosphorylated peptide supported in the decellularized matrix hydrogel. In any embodiment of the present application, the concentration of the 7-amino acid phosphorylated peptide in the hydrogel for repairing a neurological deficit is 30 μg/ml to 70 μg/ml based on the total mass of the hydrogel for repairing a neurological deficit. In any embodiment of the application, the 7-amino acid phosphorylated peptide repairs the neurological deficit by at least one of the following mechanisms: (1) Promoting polarization of macrophages to the anti-inflammatory phenotype M2; (2) Promoting vascular matrix network remodeling, wherein promoting vascular matrix network remodeling comprises at least one of enhancing proliferative activity of vascular endothelial cells and promoting migration of vascular endothelial cells; (3) Enhancing the function of the schwann cells, wherein enhancing the function of the schwann cells comprises improving at least one of proliferation, migration and secretion of the schwann cells. In any embodiment of the application, the 7-amino acid phosphorylated peptide is supported in the acellular matrix hydrogel by at least one of covalent bonding, non-covalent bonding, and physical entrapment. In any embodiment of the application, the acellular matrix hydrogel comprises acellular matrix, and the concentration of acellular matrix in the acellular matrix hydrogel is 0.3% (w/v) to 0.9% (w/v). In a second aspect, the application provides the use of a 7-amino acid phosphorylated peptide in the manufacture of a product for repairing a neurological deficit. In any embodiment of the application, the product comprises at least one of (1) hydrogel, (2) three-dimensional scaffold, and (3) kit. In any embodiment of the application, the 7-amino acid phosphorylated peptide repairs the neurological deficit by at least one of (1) promoting polarization of macrophages to the anti-inflammatory phenotype type M2, (2) promoting vascular matrix network remodeling, wherein promoting vascular matrix network remodeling comprises at least one of enhancing proliferation activity of vascular endothelial cells and promoting migration of vascular endothelial cells, and (3) enhancing schwann cell function, wherein enhancing schwann cell function comprises at least one of improving proliferation capacity, migration capacity, and secretory function of schwann cells. In any embodiment of the application, the neurological deficit includes at least one of a spinal nerve injury, a cranial nerve injury, and an autonomic nerve injury. In any embodiment of the application, the therapeutically effective amount of 7-amino acid phosphorylated peptide is 0.002 mg/kg body weight to 0.008 mg/kg body weight. The hydrogel containing 7-amino acid phosphorylated peptide and the application thereof in nerve repair, provided by the embodiment of the application, can play a multi-dimensional regulation role in nerve injury microenvironment remodeling, and realize dynamic targeted regulation and control on peripheral nerve injury microenvironment by promoting proliferation capacity of schwann cells, promoting expression of nerve-related trophic factors in the schwann cells, promoting polarization of macrophages to repair phenotypes, promoting regeneration of blood vessels and the like. Drawings In order to more clearly illustrate the technical solution of the embodiments of the present application, the drawings that are needed to be used in the embodiments of the present application will be briefly described, and it is possible for a person skilled in the art to obtain other drawings according to these drawings