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CN-121971707-A - Acellular matrix fiber woven scaffold and preparation method and application thereof

CN121971707ACN 121971707 ACN121971707 ACN 121971707ACN-121971707-A

Abstract

The invention provides a decellularized matrix fiber woven stent, a preparation method and application thereof, and belongs to the technical field of biomedical materials. The acellular matrix fiber weaving bracket comprises a weaving structural unit, wherein the weaving structural unit comprises warp threads and a plurality of acellular matrix fiber wefts, the acellular matrix fibers are manufactured by equidistant cutting of acellular matrix paper-based materials, the acellular matrix fiber wefts are arranged in parallel according to a bionic trend and are interwoven and fixed through the warp threads, and therefore bionic patterns simulating natural tissue fiber arrangement are formed in the weaving structural unit. The invention creatively applies the weaving technology to prepare the decellularized matrix fiber scaffold, can improve the cell loading efficiency, is beneficial to self-repairing and reconstructing damaged tissues, provides proper survival and growth conditions for cells, and has great application potential in the field of biomedical engineering, in particular in the aspects of tissue repairing and regenerating medicine.

Inventors

  • ZHU MEIFENG
  • SONG GUANGZHOU

Assignees

  • 纽生(天津)生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260209

Claims (10)

  1. 1. A woven decellularized matrix fiber scaffold comprising a woven structural unit comprising a warp and a plurality of decellularized matrix fiber wefts; the acellular matrix fiber weft is prepared by equidistant cutting of acellular matrix paper-based materials; The plurality of acellular matrix fiber wefts are arranged in parallel according to the bionic trend and are interwoven and fixed through the warps, so that a bionic pattern simulating natural tissue fiber arrangement is formed in the weaving structural unit.
  2. 2. The decellularized matrix fiber woven scaffold of claim 1, wherein said decellularized matrix fiber has a width of 1-2000 μm and a length of 1-100000 μm.
  3. 3. The decellularized matrix fiber woven stent of claim 1, wherein said warp and weft interweaves in any of an orthogonal, plain, twill, or satin weave.
  4. 4. The decellularized matrix fiber woven scaffold of claim 1, wherein said biomimetic trend is an alignment direction simulating skeletal muscle fascicles, tendon fascicles, nerve fascicles, or vascular smooth muscle cell orientation.
  5. 5. The acellular matrix fiber woven scaffold of claim 1, wherein the acellular matrix fiber woven scaffold is formed of two or more layers of the woven structural units stacked to form a three-dimensional structure.
  6. 6. A method of making the decellularized matrix fiber woven scaffold of any of claims 1-5, comprising the steps of: Equidistant cutting is carried out on the acellular matrix paper-based material to obtain acellular matrix fibers; arranging a plurality of acellular matrix fibers in parallel according to a preset bionic trend to serve as wefts; fixing the warp yarns in an interweaving manner to form a single-layer weaving structure unit with a bionic pattern; stacking at least one layer of the single-layer weaving structural units to obtain the acellular matrix fiber weaving bracket.
  7. 7. The method for preparing the acellular matrix fiber woven scaffold according to claim 6, wherein the acellular matrix paper-based material is prepared by homogenizing, filtering and airing the acellular matrix material.
  8. 8. Use of the acellular matrix fiber woven scaffold of any one of claims 1-5 in the preparation of tissue engineering scaffold materials.
  9. 9. The use according to claim 8, wherein the tissue is skeletal muscle, tendon, ligament, skin or fascia.
  10. 10. A tissue repair product comprising the decellularized matrix fiber woven scaffold of any of claims 1-5, and an active ingredient supported thereon.

Description

Acellular matrix fiber woven scaffold and preparation method and application thereof Technical Field The invention belongs to the technical field of biomedical materials, and particularly relates to a decellularized matrix fiber woven scaffold, a preparation method and application thereof. Background The biological material has important application in the field of tissue repair and regeneration, and can realize the replacement and repair of damaged and degenerated tissues. From the aspect of performance requirements, the biological material needs to have a proper pore structure, aims to provide stable physical structural support for the growth of cells and tissues, and simultaneously has excellent biocompatibility, bioactivity and mechanical properties. Currently, in the course of research on tissue regeneration and repair materials, a series of efforts have been made, mainly covering synthetic polymer materials such as PolyActive < lambda > developed by Innoskel, collagen materials such as INTEGRALIFESCIENCES, intego DermalRegenerationTemplate, and decellularized matrix materials such as ACell, MATRISTEM < lambda > and the like. In particular, synthetic polymeric materials exhibit high mechanical strength and are easy to process and shape, however, they suffer from significant drawbacks in that they suffer from lack of bioactivity, resulting in limited ability to promote tissue repair. Although the collagen has good biocompatibility, the collagen has poor mechanical properties and poor processability. The decellularized matrix material has outstanding biocompatibility and bioactivity, can build a physical supporting environment for cells, transmit biochemical signals and provide a dynamic regulation environment, and is suitable for in vivo implantation to promote physiological remodeling of tissues. However, it should be emphasized that the structure of the acellular matrix material obtained by directly performing the decellularization treatment on the tissue exhibits a compact nature, which limits the migration of endogenous cells to a certain extent, thereby negatively affecting the in situ regeneration and repair ability of the tissue. The only clinical trial worldwide dominated by university of pittsburgh in the united states uses decellularized porcine-derived matrix membrane material (e.g., bladder/small intestine submucosa) in combination with physical therapy to repair war wound muscle defects, but only to effect scar repair without reconstructing functional muscle tissue. This highlights the inherent limitations of using acellular matrix sheets alone in directing functional tissue regeneration. To improve the structure and performance of decellularized matrix materials, the prior art has been developed mainly in two directions: The first type of process relies on chemical deconstruction and recombination. Many studies have introduced diverse processes such as electrospinning, 3D printing, and hydrogels, with the aim of building porous or customized structures. Although these processes contribute to some extent to the improvement of the morphology of the material, they all have a common problem in that during the implementation of these processes, it is generally necessary to use acid solutions, enzyme solutions or organic solvents in order to achieve the purpose of lysis or digestion of the acellular matrix. However, this treatment inevitably results in damage or loss of extracellular matrix components, which in turn results in a significant decrease in the biological activity of the decellularized matrix material. The second type of process attempts to perform structural remodeling using purely physical methods. For example, a process similar to paper making is disclosed, wherein the decellularized matrix is homogenized, filtered, and dried to produce porous tissue paper (e.g., CN114984320 a). The method avoids the destruction of chemical reagents, but the formed structure is essentially a two-dimensional random porous sheet, lacks an anisotropic structure which has a guiding effect on cell growth and imitates the arrangement of natural tissue fibers, and is difficult to realize the complete reconstruction of functions when repairing highly ordered tissues such as muscles, tendons and the like. Another technical solution (such as CN118512659 a) attempts to make a tubular decellularized tissue into continuous fibers by rotary cutting and twisting, and further braiding into a tubular scaffold. This approach, while avoiding chemical dissolution and achieving fibrotic knitting, is limited in its process to specific tubular tissue sources and its knitting purpose is primarily to construct macroscopic tubular morphology rather than to mimic the fiber alignment of specific tissues (e.g., muscle bundles) on a microscopic or mesoscopic scale to guide cell directed regeneration. For the repair of large numbers of non-tubular, structurally ordered soft tissue defects (e.g., volumetric muscle defects, tendo