Search

CN-121971715-A - Asymmetric anti-adhesion composite membrane and preparation method and application thereof

CN121971715ACN 121971715 ACN121971715 ACN 121971715ACN-121971715-A

Abstract

The application provides an asymmetric anti-adhesion composite membrane, a preparation method and application thereof, wherein the composite membrane comprises a bioactive layer and an electrospun fiber layer, the electrospun fiber layer comprises a first fiber layer and a second fiber layer, the first fiber layer comprises fibers with a first diameter, the second fiber layer comprises fibers with a first diameter and fibers with a second diameter, and the first diameter is larger than the second diameter. The composite membrane has strong mechanical and degradation resistance, is suitable for anti-adhesion application of various movable organs or tissues, can still have structural integrity and functional integrity for a long time under the environments, has adhesive fibers on one side, has stronger adhesive force under wet conditions, and can ensure that the composite membrane cannot easily shift when the movable organs or tissues are applied.

Inventors

  • ZHANG XIAOBIN
  • CHEN FENG
  • ZHONG WENDE
  • LIANG HUIHUI
  • MO ZIRU

Assignees

  • 冠昊生物科技股份有限公司

Dates

Publication Date
20260505
Application Date
20260408

Claims (10)

  1. 1. The asymmetric anti-adhesion composite membrane is characterized by comprising a bioactive layer and an electrospun fiber layer, wherein the electrospun fiber layer comprises a first fiber layer and a second fiber layer, and the bioactive layer, the first fiber layer and the second fiber layer are sequentially arranged; The first fibrous layer comprises fibers of a first diameter and the second fibrous layer comprises fibers of a first diameter and fibers of a second diameter, the first diameter being greater than the second diameter.
  2. 2. The asymmetric antiblocking composite membrane of claim 1 wherein, The diameter of the first diameter fiber is in the range of 0.5-2 mu m, and the diameter of the second diameter fiber is in the range of 0.2-0.5 mu m.
  3. 3. The asymmetric antiblocking composite membrane of claim 1 wherein, The number ratio of the fibers of the first diameter to the fibers of the second diameter in the second fiber layer is (1-3): 1.
  4. 4. The asymmetric antiblocking composite membrane of claim 1 wherein, The material of the fiber with the first diameter is one or a combination of more than two of polylactic acid, polymethyl methacrylate, polyvinylidene fluoride, polyurethane or polycaprolactone; The material of the second diameter fiber is polyvinylpyrrolidone.
  5. 5. The asymmetric antiblocking composite membrane of claim 1 wherein, The thickness of the bioactive layer is 0.5-1mm; the bioactive layer is made of one or more of collagen, silk fibroin, oxidized cellulose, chitosan or hyaluronic acid.
  6. 6. The asymmetric antiblocking composite membrane of claim 1 wherein, The bioactive layer is a collagen layer, and the collagen is prepared from animal Achilles tendon tissue through the steps of enzymolysis extraction, salting out, crosslinking and protein fixation.
  7. 7. The asymmetric antiblocking composite membrane of claim 6 wherein the polymeric material comprises, The enzyme used for enzymolysis is a mixed enzyme of pepsin and lipase, and the mass ratio of the pepsin to the lipase is (1-3): 1; Protein immobilization is performed in glycine solution; the degree of crosslinking is 5-10%.
  8. 8. The method for preparing the asymmetric anti-blocking composite membrane according to any one of claims 1 to 7, comprising the steps of: The method comprises the steps of simultaneously carrying out electrostatic spinning on fibers with a first diameter and fibers with a second diameter on a substrate to form a second fiber layer, stopping the electrostatic spinning of the fibers with the second diameter, continuing to carry out electrostatic spinning on the fibers with the first diameter on the second fiber layer to form the second fiber layer, loading a bioactive layer on one side of the second fiber layer, and carrying out vacuum drying to obtain the asymmetric anti-adhesion composite membrane.
  9. 9. The method for preparing the asymmetric anti-adhesion composite membrane according to claim 8, wherein, The preparation condition of the electrospun fiber comprises that the spinning needle is 21G, the temperature of the spinning environment is 35-40 ℃, the relative humidity is 35-45%RH, the distance between the spinning needle and a collector is 20cm, the spinning solution is injected at the rate of 1mL/h, the positive voltage of the first diameter fiber spinning needle is set to +16kV, and the positive voltage of the second diameter fiber spinning needle is set to +14kV.
  10. 10. Use of an asymmetric anti-adhesion composite membrane according to any one of claims 1 to 9 for organ/tissue anti-adhesion.

Description

Asymmetric anti-adhesion composite membrane and preparation method and application thereof Technical Field The application belongs to the technical field of biological membrane materials, and particularly relates to an asymmetric anti-adhesion composite membrane, and a preparation method and application thereof. Background The existing materials for organ/tissue anti-adhesion application are mainly gel or film, wherein the gel materials are mainly prepared by directly injecting polymer solution such as sodium hyaluronate gel, polylactic acid gel or chitosan gel into adhesion parts, the liquid gel materials can also be prepared into films, and a gel is formed after being wetted by liquid before application and is adhered to the adhesion parts, so that the anti-adhesion effect is finally achieved. In addition, some polymer materials, such as animal-derived collagen, oxidized cellulose (e.g., carboxymethyl cellulose) or chitosan, can be made into anti-blocking materials by lyophilization or air-drying to form a film. Some films prepared based on braiding or electrospinning processes have also begun to be explored for use in antiblocking applications. Current anti-adhesion films for organ or tissue anti-adhesion applications suffer from the following drawbacks/problems: (1) Lack of adequate adhesion, in some sports organoid adhesion scenarios, shift easily occurs during use; (2) The (woven/electrospun) fiber-based material is prone to cell adhesion and stretching due to the rough surface and porous nature, and eventually tends to cause strong tissue adhesion; (3) The problem of rapid degradation and mechanical property reduction of the film formed by air-drying the polymer solution exists in the presence of tissue fluid. Disclosure of Invention The embodiment of the application provides an asymmetric anti-adhesion composite membrane, a preparation method and application thereof, which are used for solving the problems of the related technology, and the technical scheme is as follows: In a first aspect, the embodiment of the application provides an asymmetric anti-adhesion composite membrane, which comprises a bioactive layer and an electrospun fiber layer, wherein the electrospun fiber layer comprises a first fiber layer and a second fiber layer; The first fibrous layer comprises fibers of a first diameter and the second fibrous layer comprises fibers of a first diameter and fibers of a second diameter, the first diameter being greater than the second diameter. In one embodiment, the first diameter fibers have a diameter in the range of 0.5 to 2 μm and the second diameter fibers have a diameter in the range of 0.2 to 0.5 μm. In one embodiment, the number ratio of fibers of the first diameter to fibers of the second diameter in the second fiber layer is (1-3): 1. In one embodiment, the material of the first diameter fiber is one or a combination of more than two of polylactic acid, polymethyl methacrylate, polyvinylidene fluoride, polyurethane or polycaprolactone. In one embodiment, the material of the second diameter fibers is polyvinylpyrrolidone. In one embodiment, the bioactive layer has a thickness of 0.5-1mm. In one embodiment, the material of the bioactive layer is one or a combination of more than two of collagen, silk fibroin, oxidized cellulose, chitosan or hyaluronic acid. In one embodiment, the bioactive layer is a collagen layer, and the collagen is prepared from animal Achilles tendon tissue by steps including enzymatic extraction, salting-out, crosslinking and protein fixation. In one embodiment, the enzyme used for enzymolysis is a mixed enzyme of pepsin and lipase, and the mass ratio of the pepsin to the lipase is (1-3): 1. In one embodiment, the protein immobilization is performed in glycine solution. In one embodiment, the degree of crosslinking is from 5 to 10%. In a second aspect, embodiments of the present application provide a method for preparing an asymmetric anti-blocking composite membrane, comprising the steps of: The method comprises the steps of simultaneously carrying out electrostatic spinning on fibers with a first diameter and fibers with a second diameter on a substrate to form a second fiber layer, stopping the electrostatic spinning of the fibers with the second diameter, continuing to carry out electrostatic spinning on the fibers with the first diameter on the second fiber layer to form the second fiber layer, loading a bioactive layer on one side of the second fiber layer, and carrying out vacuum drying to obtain the asymmetric anti-adhesion composite membrane. In one embodiment, the electrospun fiber is prepared under conditions of 21G spinning needle, 35-40 deg.C spinning environment temperature, 35-45% RH relative humidity, 20cm distance from the collector, 1mL/h injection of spinning solution, positive electrode voltage of the first diameter fiber spinning needle set to +16kV, and positive electrode voltage of the second diameter fiber spinning needle set to +14kV. In