CN-121971909-A - Intelligent suction filtration method and device for liquid biological sample

CN121971909ACN 121971909 ACN121971909 ACN 121971909ACN-121971909-A

Abstract

The invention provides an intelligent suction filtration method and device for a liquid biological sample, which solve the problem of lower suction filtration efficiency of the conventional suction filtration system for the biological fluid sample. The multi-station rotary platform is provided with a plurality of filtering boxes which are arranged at intervals in a circumferential direction, the top of each filtering box is provided with a sample inlet, a filtering membrane is arranged in each filtering box, each filtering box can be disassembled to take out the filtering membrane in each filtering box, the bottom of each filtering box is provided with a liquid outlet, the sample injection device comprises a first lifting mechanism and a plurality of sample injection tube heads which are fixedly arranged at the bottom of the first lifting mechanism, each sample injection tube head can be inserted into the sample inlet of each filtering box on the multi-station rotary platform, each filtrate suction device comprises a second lifting mechanism and a plurality of liquid extraction tube heads which are fixedly arranged at the top of the second lifting mechanism, and each liquid extraction tube head can be inserted into the liquid outlet of each filtering box on the multi-station rotary platform.

Inventors

FU ABAO
GAO YUAN
ZHAO TAO
TIAN SHIQIANG

Assignees

复崟(上海)科技有限公司

Dates

Publication Date: 20260505
Application Date: 20260204

Claims (10)

1. The intelligent suction filtration device for the liquid biological sample is characterized by comprising a box body, wherein a control panel and a front-back transparent taking and placing opening are arranged on the outer side of a front panel of the box body; A sample injection device, a multi-station rotating platform and a filtrate suction device are sequentially arranged in the box body from top to bottom; A plurality of filter boxes which are arranged at intervals in the circumferential direction are inserted into the multi-station rotary platform, and the multi-station rotary platform can drive the filter boxes to horizontally rotate; The top of the filter box is provided with an up-down through sample inlet, a first filter screen and a second filter screen which are arranged at intervals up and down are arranged in the filter box, a filter membrane is arranged between the first filter screen and the second filter screen, the filter box can be disassembled to take out the filter membrane therein, and the bottom of the filter box is provided with an up-down through liquid outlet; The sample injection device comprises a first lifting mechanism and a plurality of sample injection tube heads fixedly arranged at the bottom of the first lifting mechanism, the top of each sample injection tube head is communicated with the bottom of a sample storage tank through a hose, the sample storage tank is arranged in a box body, and each sample injection tube head can be inserted into a sample injection port of a filter cartridge on the multi-station rotary platform; the filtrate suction device comprises a second lifting mechanism and a plurality of liquid suction tube heads fixedly arranged at the top of the second lifting mechanism, the bottoms of the liquid suction tube heads are connected with the negative pressure filtrate collecting device through hoses, and the liquid suction tube heads can be inserted into liquid discharge ports of the filter boxes on the multi-station rotating platform; the control panel is respectively connected with the sample injection device, the multi-station rotating platform and the filtrate suction device in a control way.
2. The intelligent suction filtration device for liquid biological samples according to claim 1, wherein a vertical frame is arranged in the box body and positioned behind the rotary supporting device, and a sample feeding device, a multi-station rotary platform and a filtrate suction device are sequentially arranged on the vertical frame from top to bottom; The ceramic oscillator is arranged in the box body, the lower end of the vertical frame is fixedly arranged at the top of the ceramic oscillator, and the ceramic oscillator is in control connection with the control panel.
3. The intelligent suction filtration device for liquid biological samples according to claim 1, wherein the inlet of the negative pressure filtrate collecting device is connected with one end of a liquid inlet pipe, a first electromagnetic valve and a flow sensor are arranged on the liquid inlet pipe, one end of the liquid inlet pipe far away from the negative pressure filtrate collecting device is connected with a distribution box, the inside of the distribution box is of a hollow structure, the right side of the distribution box is provided with a plurality of third pipe connectors communicated with the inside of the distribution box, and the third pipe connectors are connected with the liquid suction pipe heads one by one through hoses; The liquid inlet pipe between the first electromagnetic valve and the distribution box is connected with a reverse pulse device which is used for unidirectionally feeding gas into the liquid inlet pipe.
4. The intelligent suction filtration device for liquid biological samples of claim 3, wherein the reverse pulse device comprises an air pump, an air outlet of the air pump is connected with one end of an air pipe, a fourth electromagnetic valve and a one-way valve are arranged on the air pipe, and the other end of the air pipe is connected with a liquid inlet pipe between the first electromagnetic valve and the distribution box.
5. The intelligent suction filtration device for liquid biological samples of claim 1, wherein the filtration cartridge comprises an upper cartridge and a lower cartridge which are detachably connected, the top of the upper cartridge is provided with an up-down through sample inlet, the inner side of the lower end of the upper cartridge is fixedly provided with a first filter screen, the bottom of the lower cartridge is provided with an up-down through liquid outlet, the inner side of the upper end of the lower cartridge is fixedly provided with a second filter screen, and the top of the second filter screen is paved with a removable filter membrane; The sample inlet and the liquid outlet are respectively provided with a puncturable sealing film, the top of the upper box cylinder is provided with an upper and a lower permeable ventilation openings, and the inside of the ventilation openings is provided with a waterproof ventilation film.
6. The intelligent suction filtration device for liquid biological samples according to claim 1 or 5, wherein a rotary supporting device is arranged in the box body, and a plurality of filter boxes are arranged on the rotary supporting device; The multi-station rotary platform is provided with a plurality of filtering box slots which are circumferentially arranged at intervals, a box body is internally provided with a box taking mechanism which can rotate in a horizontal plane, and the box taking mechanism is used for grabbing a filtering box and placing the filtering box on the rotary supporting device or in the filtering box slots on the multi-station rotary platform; the circumference of the filter box is fixedly provided with a first limiting plate in the circumferential direction, and the outer diameter of the first limiting plate is larger than the inner diameter of the filter box slot.
7. The intelligent suction filtration device for liquid biological samples according to claim 6, wherein two annular second limiting plates which are arranged at intervals up and down are fixedly arranged on the peripheral side surface of the filter cartridge, and the outer diameter of the second limiting plates is smaller than or equal to the inner diameter of the filter cartridge slot; The bottom of the multi-station rotary platform is provided with a plurality of third telescopic devices respectively corresponding to the filter cartridge slots, clamping plates are fixedly arranged at the movable ends of the third telescopic devices, and the third telescopic devices can push the clamping plates between two second limiting plates on the filter cartridges inserted into the filter cartridge slots.
8. The intelligent suction filtration device for liquid biological samples according to claim 2, wherein the first lifting mechanism comprises a fourth telescopic device which is vertically arranged, the top of the fourth telescopic device is fixedly connected with a fourth bracket, and one end of the fourth bracket is fixedly connected with a vertical frame; The lower end of the fourth telescopic device is fixedly provided with a fourth motor, an output shaft of the fourth motor is fixedly sleeved with a second sleeve, and the circumferential side surface of the second sleeve is connected with a plurality of sample injection tube heads which are distributed at intervals along the circumferential direction of the second sleeve; the second lifting mechanism comprises a fifth telescopic device which is vertically arranged, the lower end of the fifth telescopic device is fixedly connected with the bottom of the vertical frame of the L-shaped plate structure, a fifth motor is fixedly arranged at the upper end of the fifth telescopic device, a third shaft sleeve is fixedly sleeved on an output shaft of the fifth motor, and a plurality of liquid pumping tube heads which are distributed along the circumferential direction at intervals are connected to the circumferential side surface of the third shaft sleeve.
9. The intelligent suction filtration device for liquid biological samples of claim 8, wherein the inlet of the sample injection tube head is connected with the bottom of the first buffer tank, the first buffer tank is communicated with the inside of the sample injection tube head, the top of the first buffer tank is connected with the bottom of the sample storage tank through a hose, and a fifth electromagnetic valve is arranged on a connecting pipeline of the first buffer tank and the sample storage tank; The bottom of the first buffer tank is fixedly provided with a first sealing ring which is arranged around the sample injection tube head and is used for being pressed on the top of the filter cartridge; The exit of drawing liquid tube head is connected with the top of second buffer memory jar, and the inside intercommunication each other of second buffer memory jar and drawing liquid tube head, and the second buffer memory jar passes through the hose and is connected with negative pressure filtrate collecting device, and the fixed second sealing washer that encircles drawing liquid tube head setting that is equipped with in top of second buffer memory jar is used for pressing to establish in the bottom of filtering the casket.
10. The intelligent suction filtration method for liquid biological samples according to claims 1-9, comprising the steps of firstly, correspondingly placing the filter cartridges on a second tray; Step two, placing a second tray and a filter box on the rotary supporting device through the taking and placing opening; step three, the control panel controls the box taking mechanism to take out and move the filter box on the rotary supporting device and then place the filter box in the filter box slot of the multi-station rotary platform; step four, the control panel controls the rotary supporting device to rotate, one of the filter boxes which is not removed from the rotary supporting device is positioned behind the rotary supporting device, meanwhile, the control panel controls the multi-station rotary platform to rotate, one of the spare filter box slots on the multi-station rotary platform faces forwards, the step three is repeated, and the second filter box is placed into the filter box slot of the multi-station rotary platform; Step five, repeating the step four until the required number of filter boxes are placed on the multi-station rotating platform; Step six, the control panel controls the third telescopic device to extend out, and the clamping plate is pushed between the upper limiting plate and the lower limiting plate so as to limit the up-and-down movement of the filter cartridge; A fourth motor on the sample injection device is controlled by the control panel to rotate a certain angle, so that the sample injection tube head is positioned right above a sample injection port of the filter cartridge, and then a third telescopic device on the sample injection device is controlled to push the sample injection tube head to move downwards, so that the sample injection tube head pierces a sealing film in the sample injection port and enters the filter cartridge, and a first sealing ring is pressed on the top of the filter cartridge; Simultaneously, the control panel controls the fifth motor of the filtrate sucking device to rotate for a certain angle, so that the liquid sucking pipe head is positioned under the liquid outlet of the filter box, and then controls the fifth telescopic device of the filtrate sucking device to push the liquid sucking pipe head to move upwards, so that the liquid sucking pipe head pierces the sealing film in the liquid outlet and enters the filter box, and the second sealing ring is pressed at the bottom of the filter box; Step eight, injecting a biological fluid sample into a sample storage tank, opening a fifth electromagnetic valve and a negative pressure filtrate collecting device in a first buffer tank through a control panel, sequentially passing the biological fluid sample flowing into the first buffer tank through a first filter screen, a filter membrane and a second filter screen in a filter cartridge under the negative pressure suction effect provided by the negative pressure filtrate collecting device, and then sucking filtrate into the negative pressure filtrate collecting device through a filtrate sucking device; step nine, in the process of carrying out the step eight, starting a ceramic oscillator through a control panel at fixed time, and oscillating a sample feeding device, a multi-station rotating platform and a filtrate sucking device; The control panel is used for closing the first electromagnetic valve at fixed time, opening the reverse pulse device, and sending gas to the liquid inlet pipe in one way at intervals through the reverse pulse device so as to disturb the liquid in the liquid inlet pipe and the filtrate suction device, and closing the first electromagnetic valve again after the completion of the disturbance, and starting the first electromagnetic valve; Step ten, after the suction filtration is completed, the fourth telescopic device and the fifth telescopic device are controlled to shrink through the control panel so as to pull the sample injection tube head and the liquid suction tube head out of the filter box, and then the fourth motor and the fifth motor are controlled to rotate for a certain angle, so that the sample injection tube head and the liquid suction tube head are staggered with a filter box slot on the multi-station rotary platform; And step eleven, controlling a box taking mechanism through a control panel, referring to the step three, the step four and the step five, sequentially placing the filter boxes on the multi-station rotary platform on a rotary supporting device, taking out the filter boxes through a taking-out and placing port, disassembling the filter boxes, taking out a filter membrane carrying protein, and drying the filter membrane to obtain a dry film biological fluid sample.

Description

Intelligent suction filtration method and device for liquid biological sample Technical Field The invention relates to the technical field of biological sample treatment, in particular to an intelligent suction filtration method and device for a liquid biological sample. Background Hospitals have a large number of biological fluid tests each year, but since biological fluid samples cannot be stored for a long period of time in normal environments, a large number of valuable samples are disposed of. At present, the biological fluid is high in preservation cost, high in technical difficulty and large in occupied space, so that a biological fluid sample library cannot be established. If the biological fluid sample is stored by a method, a biological fluid sample library which can be effectively stored for a long time is established, the biological fluid sample can be extracted and analyzed at any time, and the method has very important significance for screening, preventing, accurately diagnosing and predicting the diseases of individuals and improving the health of the whole people. Researchers study that biological fluid is manually filtered through a filter membrane, protein in the biological fluid is reserved on the filter membrane, the filter membrane is dried to prepare a dry film biological fluid sample, and after vacuum plastic packaging, the biological fluid can be reliably stored for a long time for subsequent analysis. Among the prior art, patent application document that application number is 202210451133.5 discloses a suction filtration system based on biological fluid sample, suction filtration device passes through pipeline and vacuum pump intercommunication, suction filtration device passes through O type sealing washer and sample adapter sealing connection, suction filtration device passes through flexible removal and sampling device contact or separation, sampling device passes through radial rotation and supplies sample in the sample tube to flow into the sample adapter, the sample adapter passes through the vacuum pump filters, establish ties flow sensor, waste liquid collecting vessel, pressure sensor and breather valve in proper order on the pipeline, the breather valve with the vacuum pump respectively with control system signal connection, flow sensor reaches pressure sensor transmits the signal transmission of response to control system, control system then controls respectively, the breather valve with the operating condition of vacuum pump. However, the suction filtration system has the following defects that (1) a manual adapter and an O-shaped ring are required to be assembled and manually aligned during use, the use is troublesome, and (2) the suction filtration efficiency is low, and only one filtering membrane with protein in biological fluid can be obtained during each operation, so that the preparation efficiency of a dry film biological fluid sample is low. Disclosure of Invention In order to solve the problem of low suction efficiency of the conventional suction filtration system for biological fluid samples in the background art, the invention provides an intelligent suction filtration method and device for liquid biological samples. The intelligent suction filtration device for the liquid biological sample comprises a box body, wherein a control panel and a front-back transparent taking and placing port are arranged on the outer side of a front panel of the box body; A sample injection device, a multi-station rotating platform and a filtrate suction device are sequentially arranged in the box body from top to bottom; A plurality of filter boxes which are arranged at intervals in the circumferential direction are inserted into the multi-station rotary platform, and the multi-station rotary platform can drive the filter boxes to horizontally rotate; The top of the filter box is provided with an up-down through sample inlet, a first filter screen and a second filter screen which are arranged at intervals up and down are arranged in the filter box, a filter membrane is arranged between the first filter screen and the second filter screen, the filter box can be disassembled to take out the filter membrane therein, and the bottom of the filter box is provided with an up-down through liquid outlet; The sample injection device comprises a first lifting mechanism and a plurality of sample injection tube heads fixedly arranged at the bottom of the first lifting mechanism, the top of each sample injection tube head is communicated with the bottom of a sample storage tank through a hose, the sample storage tank is arranged in a box body, and each sample injection tube head can be inserted into a sample injection port of a filter cartridge on the multi-station rotary platform; the filtrate suction device comprises a second lifting mechanism and a plurality of liquid suction tube heads fixedly arranged at the top of the second lifting mechanism, the bottoms of the liquid suction tube he