CN-121972144-A - Method for separating and purifying pericarpium Granati polyphenol based on metal organic framework material

Abstract

The invention provides a method for separating and purifying pericarpium Granati polyphenol based on a metal organic framework material, belonging to the technical field of natural product extraction. The preparation method comprises the steps of (1) mixing a magnetic metal organic frame material with a crude extract of pericarpium Granati polyphenol, stirring for 50-70 min, then carrying out solid-liquid separation to obtain a magnetic metal organic frame material loaded with pericarpium Granati polyphenol, (2) adding an eluting solvent into the magnetic metal organic frame material loaded with pericarpium Granati polyphenol, stirring, standing, carrying out solid-liquid separation to obtain a polyphenol eluent and precipitate, and (3) concentrating and drying the polyphenol eluent to obtain high-purity pericarpium Granati polyphenol. The invention overcomes the inherent defects of the traditional resin in selectivity and dynamics by utilizing a special regular pore canal system and metal active sites of the MOF material, and provides a better technical path for the deep development of the pericarpium Granati waste.

Inventors

• Di Li Re Ba Shatar
• CAO SIYU
• ZHANG MINWEI
• Akbar Jumai
• ZHAO JIUZHOU

Assignees

• 新疆大学

Dates

Publication Date

20260505

Application Date

20260310

Claims (10)

1. 1. The method for separating and purifying the pericarpium Granati polyphenol based on the metal organic framework material is characterized by comprising the following steps of: (1) Mixing the magnetic metal organic frame material with the crude extract of the pericarpium Granati polyphenol, stirring for 50-70 min, and then carrying out solid-liquid separation to obtain the magnetic metal organic frame material carrying the pericarpium Granati polyphenol; (2) Adding an eluting solvent into the magnetic metal organic framework material loaded with the pericarpium granati polyphenol, stirring, standing, and performing solid-liquid separation to obtain polyphenol eluent and precipitate; (3) Concentrating and drying the polyphenol eluent to obtain high-purity pericarpium Granati polyphenol.
2. 2. The method of claim 1, wherein the magnetic metal organic framework material is a composite adsorption medium having a magnetic core and a MOF shell structure, the magnetic core being Fe 3 O 4 .
3. 3. The method of claim 2, wherein the magnetic metal-organic framework material is Fe 3 O 4 @UiO-66-NH 2 .
4. 4. The method of claim 1, wherein the mass to volume ratio of the magnetic metal organic framework material to the crude pericarpium Granati polyphenol extract is 1 mg:4-6 ml.
5. 5. The method of claim 1, wherein the preparation method of the crude extract of pericarpium Granati polyphenol comprises mixing pericarpium Granati powder with 40% -60% ethanol solution, reflux-extracting for 2-4 times, mixing filtrates, and concentrating by rotary evaporation until no alcohol smell is present to obtain the crude extract of pericarpium Granati polyphenol.
6. 6. The method of claim 5, wherein the mass to volume ratio of the pericarpium Granati powder to the ethanol solution is 1 g:5-10 ml.
7. 7. The method according to claim 1, wherein the solid-liquid separation is realized by collecting magnetic metal organic frame materials by using a magnet.
8. 8. The method according to claim 1, wherein the eluting solvent is an ethanol solution with an initial concentration of 50% -70%, and the volume-mass ratio of the eluting solvent to the magnetic metal organic framework material is 0.5-1.5 ml/1 mg.
9. 9. The method of claim 1, further comprising the step of recovering the precipitate obtained in step (2).
10. 10. The method of claim 9, wherein the recovering is performed by alternately washing the precipitate with absolute ethanol and deionized water to recover the adsorptive activity of the magnetic metal organic framework material.

Description

Method for separating and purifying pericarpium Granati polyphenol based on metal organic framework material Technical Field The invention belongs to the technical field of natural product extraction, and particularly relates to a method for separating and purifying pericarpium Granati polyphenol based on a metal organic framework material. Background Pomegranate (Punica granatum) has been attracting attention since ancient times and is an economically important plant, and has been known as a "super food" because of its great potential for health benefits. The popularity and increasing demand of pomegranates is mainly due to their multifunctional and health promoting effects. The whole fruit has antibacterial, antioxidant, anticancer, antidiabetic and antiinflammatory activities. The pomegranate fruit comprises three parts, namely seeds, juice and pericarps. 40% -50% of the total weight of the pomegranate Pi Yaozhan. Considerable amounts of phenols, flavonoids and mono Ningdu are present in the peel of punica granatum fruits, and thus various biological properties and potential values of punica granatum are associated with the peel of punica granatum. However, in food processing, pericarp and seed are mostly considered as fruit juice processing byproducts, often used as animal feed, disposed of in landfill or burned. The proliferation of byproducts from fruit and vegetable processing has become a global priority to drive sustainable, economically viable, and environmentally friendly waste management strategies. The extraction of natural polyphenol antioxidants rich in the pomegranate rind is a core strategy for realizing the high-valued utilization of the agricultural byproducts. The recovery and development of the bioactive compounds not only provides a material basis for the innovation of functional foods and nutritional health care products so as to cope with the health challenges and nutritional requirements of the modern society, but also suppresses the generation of environmental wastes from the source. By constructing the circular economy mode, the path effectively promotes the transformation of the agricultural system to a sustainable direction, and realizes the efficient recycling and reutilization of resources. Purification of polyphenol active compounds from crude extracts is critical to research and production, and conventional polyphenol separation techniques mainly rely on methods such as solid-liquid extraction, liquid-liquid extraction and the like. However, these methods have the defects of high consumption of organic solvents, poor safety, low production efficiency and the like, and are difficult to meet the requirement of large-scale industrialization. In contrast, the most commonly used separation scheme for pericarpium Granati polyphenols in industry and academia is to use macroporous adsorption resin (Macroporous Adsorption Resin, MAR for short). The technology is used as a durable polymer with high adsorption capacity, covers various types of polar, nonpolar, micro-hydrophilic and the like, and has been widely applied to the industrialized recovery of plant active substances by virtue of the remarkable advantages of high mechanical strength, various structures, low cost, environmental friendliness and the like. The technology mainly utilizes the high specific surface area and the porous structure of the resin, and the polyphenol substances in the extracting solution are adsorbed on the surface of the resin through the Van der Waals force and weak interaction between the hydrophobic matrix of the resin and nonpolar phenolic molecules, so that the efficient enrichment and separation of the polyphenol are realized. Although the above method has unique advantages, it has disadvantages of (1) low adsorption site utilization rate and lack of molecular recognition ability, resulting in difficulty in both recovery efficiency and product purity. The pore size distribution of the prior art (e.g. macroporous resin MARs) is highly heterogeneous and its adsorption mechanism relies mainly on single, nondirectional hydrophobic interactions (Van der Waals forces). This adsorption mode results in materials lacking precise size matching and chemical affinity for pericarpium Granati polyphenols (e.g., punicalagin of huge molecular weight), large number of internal micropores not being effectively utilized, and easy simultaneous adsorption of impurities (e.g., sugars, organic acids). Eventually, the saturated adsorption amount of the adsorbent per unit mass is low, and the purity of the target polyphenol in the eluted product is not ideal. (2) The dynamic performance is poor, the treatment period is long, the macroporous resin is mainly micron-sized particles, the internal diffusion path is long, and the pore structure is highly distorted. The target molecules enter the adsorption sites slowly, and the time required for reaching adsorption equilibrium is long. The approximate scheme in the prior art gene