CN-121972241-A - Sample processing system

CN121972241ACN 121972241 ACN121972241 ACN 121972241ACN-121972241-A

Abstract

The invention relates to the technical field of biological sample processing, in particular to a sample processing system, which comprises a microfluidic chip, a positive pressure source and a negative pressure source, wherein the microfluidic chip comprises a reaction chamber, a first chamber which is connected with the reaction chamber and is commonly used for storing reagents, and a liquid outlet side, a filtering structure is arranged between the reaction chamber and the liquid outlet side, waste liquid output by the reaction chamber is filtered by the filtering structure and then is discharged to the liquid outlet side, the positive pressure source is used for providing positive pressure to drive the reagents in the first chamber to be pressed into the reaction chamber, the negative pressure source is used for providing negative pressure to drive the waste liquid output by the reaction chamber to flow to the liquid outlet side after being filtered by the filtering structure, and the sample reaction place is transferred onto the microfluidic chip from conventional consumables for experiments.

Inventors

ZHENG JIANFEI
LIANG JIAN
TU YANJUN
ZHANG HENG

Assignees

杭州跃真生物科技有限公司

Dates

Publication Date: 20260505
Application Date: 20241030

Claims (12)

1. A sample processing system, comprising: The microfluidic chip comprises a reaction chamber, a first chamber connected with the reaction chamber and used for storing a reagent in a universal way, and a liquid outlet side, wherein a filtering structure is arranged between the reaction chamber and the liquid outlet side, and waste liquid output by the reaction chamber is filtered by the filtering structure and then discharged to the liquid outlet side; A positive pressure source selectively connecting the first chamber to provide positive pressure to force the reagents in the first chamber into the reaction chamber; The negative pressure source is selectively connected with the liquid outlet side to provide negative pressure to drive the waste liquid output by the reaction chamber to flow to the liquid outlet side after being filtered by the filtering structure.
2. The sample processing system of claim 1, wherein said filter structure comprises one or more filter channels communicating the outlet side with the reaction chamber.
3. The sample processing system of claim 1, wherein the first chamber is in communication with the reaction chamber via a first flow path, wherein the first flow path is at least partially of a serpentine configuration and/or wherein the first flow path is at least partially of a reduced diameter configuration.
4. The sample processing system according to claim 1, wherein the positive pressure source comprises a positive pressure output end for outputting positive pressure, the first chamber comprises a first butt joint end positioned at the lower side of the positive pressure output end and used for butt joint with the positive pressure output end, a first lifting mechanism is arranged between the positive pressure output end and the microfluidic chip, and the microfluidic chip and the positive pressure output end are driven to move vertically relatively by the first lifting mechanism so as to be communicated with or separated from the first butt joint end; The negative pressure source comprises a negative pressure output end for outputting negative pressure, the liquid outlet side comprises a second chamber capable of storing waste liquid, and the second chamber comprises a second butt joint end which is positioned at the lower side of the negative pressure output end and is used for butt joint with the negative pressure output end; The negative pressure output end and the positive pressure output end can synchronously move vertically under the drive of the first lifting mechanism so as to connect or disconnect the negative pressure output end and the second butt joint end; Or the negative pressure output end is connected with a second lifting mechanism, and the negative pressure output end is driven by the second lifting mechanism to move vertically so as to be communicated or separated from the second butt joint end.
5. The sample processing system according to claim 4, wherein the negative pressure output end and the positive pressure output end move vertically synchronously, the first lifting mechanism comprises a confluence plate and a driving piece for driving the confluence plate to move vertically, and the confluence plate is provided with a first through hole forming the positive pressure output end and a second through hole forming the negative pressure output end.
6. The sample processing system of claim 1, wherein the negative pressure source comprises a negative pressure output end for outputting negative pressure, the liquid outlet side comprises a liquid outlet communicated with the reaction chamber, the liquid outlet is connected with a collecting unit for collecting waste liquid, the collecting unit comprises a third chamber for storing the waste liquid output by the liquid outlet, the negative pressure output end is communicated with the third chamber for forming the negative pressure in the third chamber, and the third chamber is communicated with the liquid outlet.
7. The sample processing system of claim 1, further comprising a grinding mechanism, wherein the grinding mechanism comprises a grinding rotor capable of rotating circumferentially and moving vertically within the reaction chamber, wherein the reaction chamber is open at the top for receiving the grinding rotor, and wherein the lower end of the grinding rotor forms the grinding end.
8. The sample processing system according to claim 7, wherein the peripheral wall of the reaction chamber is provided with a vent hole, the grinding rotor is capable of being vertically switched among a first position, a second position and a third position in the reaction chamber, the first position is above the vent hole so that the reaction chamber is communicated with the outside air pressure through the vent hole, the second position is where the grinding end of the grinding rotor is in contact with the bottom wall of the reaction chamber, the third position is where the grinding end of the grinding rotor is spaced from the bottom wall of the reaction chamber by a distance, and the grinding rotor seals the vent hole.
9. The sample processing system of claim 1, wherein the reaction chamber top opening is configured, the system further comprising a thermal cover configured to removably cover the reaction chamber top opening.
10. The sample processing system according to claim 1, further comprising a temperature control unit, wherein the microfluidic chip is disposed on the temperature control unit, the temperature control unit comprises a first temperature control seat and a second temperature control seat capable of controlling temperature independently of each other, the first chamber corresponds to the first temperature control seat, and the reaction chamber corresponds to the second temperature control seat; the micro-fluidic chip is respectively provided with a first temperature zone corresponding to the first temperature control seat and a second temperature zone corresponding to the second temperature control seat, the first chamber is positioned in the first temperature zone, the reaction chamber is positioned in the second temperature zone, and the first temperature zone and the second temperature zone are at least partially isolated from each other.
11. The sample processing system of claim 10, wherein a trench is formed at a position between the first temperature region and the second temperature region at the bottom of the microfluidic chip, and the first temperature region and the second temperature region are separated by the trench; Or the microfluidic chip comprises a first part and a second part which are mutually independent, wherein the first chamber is arranged on the first part, and the reaction chamber is arranged on the second part; Or the microfluidic chip comprises a chip main body 1a, a first cover plate 1b and a second cover plate 1c, wherein the first cover plate 1b and the second cover plate 1c are respectively arranged on the front side and the back side of the chip main body 1a, a first chamber is at least partially formed between one of the first cover plate 1b and the second cover plate 1c and the chip main body 1a, and a reaction chamber is at least partially formed between the other of the first cover plate 1b and the second cover plate 1c and the chip main body 1 a; or one of the first temperature control seat and the second temperature control seat is arranged on the upper side of the microfluidic chip, the other one of the first temperature control seat and the second temperature control seat is arranged on the lower side of the microfluidic chip, and one of the upper sides of the microfluidic chip is provided with a channel for vertically inserting the first chamber or the reaction chamber.
12. The sample processing system of claim 1, further comprising a priming unit comprising a liquid pump and a priming tip, the microfluidic chip further comprising a priming chamber, the priming tip being movable relative to the microfluidic chip to unblock or separate the priming chamber.

Description

Sample processing system Technical Field The invention relates to the technical field of biological sample processing, in particular to a sample processing system. Background In the field of life sciences research, pretreatment of biological samples is often required. Conventional steps of sample pretreatment often include reagent addition, reaction under certain conditions (suitable reaction temperature, etc.), washing, removal of waste liquid, etc., and these repeated cumbersome steps often consume a lot of time and effort of the experimenter. In this regard, the automated sample pretreatment workstations on the market can replace manual handling. However, such workstations tend to be bulky, heavy, inconvenient to transport, and more suitable for service providers with large sample throughput, and are less friendly for some end-users with tight laboratory space and low sample throughput, such as laboratories in universities and research institutions. More importantly, when the traditional automatic pipetting workstation is used for sucking waste liquid, a tissue sample (especially the tissue after wax removal and rehydration) has a certain probability of being adhered to the outer wall of the pipetting tip and being lost, and for a relatively crushed sample, the tissue sample can be directly sucked into the pipetting tip and be lost. Thus, there is a need for improvement for small, precious samples. Disclosure of Invention In order to solve at least one technical problem mentioned in the background art, an object of the present invention is to provide a sample processing system. In order to achieve the above purpose, the present invention provides the following technical solutions: the present invention provides a sample processing system comprising: The microfluidic chip comprises a reaction chamber, a first chamber connected with the reaction chamber and used for storing a reagent in a universal way, and a liquid outlet side, wherein a filtering structure is arranged between the reaction chamber and the liquid outlet side, and waste liquid output by the reaction chamber is filtered by the filtering structure and then discharged to the liquid outlet side; A positive pressure source selectively connecting the first chamber to provide positive pressure to force the reagents in the first chamber into the reaction chamber; The negative pressure source is selectively connected with the liquid outlet side to provide negative pressure to drive the waste liquid output by the reaction chamber to flow to the liquid outlet side after being filtered by the filtering structure. As an alternative embodiment of the present invention, the filtering structure includes one or more filtering channels communicating the liquid outlet side with the reaction chamber. As an alternative embodiment of the invention, the first chamber is communicated with the reaction chamber through a first flow passage, and the first flow passage is at least partially in a bent structure and/or the first flow passage is at least partially in a reduced diameter structure. As an optional implementation manner of the invention, the positive pressure source comprises a positive pressure output end for outputting positive pressure, and the first chamber comprises a first butt joint end positioned at the lower side of the positive pressure output end and used for butt joint with the positive pressure output end; The negative pressure source comprises a negative pressure output end for outputting negative pressure, the liquid outlet side comprises a second chamber capable of storing waste liquid, and the second chamber comprises a second butt joint end which is positioned at the lower side of the negative pressure output end and is used for butt joint with the negative pressure output end; The negative pressure output end and the positive pressure output end can synchronously move vertically under the drive of the first lifting mechanism so as to connect or disconnect the negative pressure output end and the second butt joint end; Or the negative pressure output end is connected with a second lifting mechanism, and the negative pressure output end is driven by the second lifting mechanism to move vertically so as to be communicated or separated from the second butt joint end. As an alternative implementation mode of the invention, the negative pressure output end and the positive pressure output end synchronously move vertically, the first lifting mechanism comprises a confluence plate and a driving piece for driving the confluence plate to move vertically, and the confluence plate is provided with a first through hole forming the positive pressure output end and a second through hole forming the negative pressure output end. As an alternative implementation mode of the invention, the negative pressure source comprises a negative pressure output end used for outputting negative pressure, the liquid outlet side comprises a liquid outlet communicated wit