CN-121972293-A - Magnetic separation column rinsing device and method

Abstract

The application discloses a magnetic sorting column rinsing device which comprises a rinsing liquid conveying component, a vacuumizing component, a gas conveying component, a moving component, a sealing cavity and a control unit, wherein the moving component is arranged in the sealing cavity and comprises a sorting column moving component and a sealing plug moving component, the sorting column moving component comprises a sorting column retainer and a first driving device, the sealing plug moving component comprises a sorting column sealing plug and a second driving device, the sorting column sealing plug comprises a sealing part and a plurality of channel interfaces, the rinsing liquid conveying component and the gas conveying component are connected to the channel interfaces through pipelines, and the vacuumizing component is connected to the sealing cavity through pipelines. The washing device can automatically wash the sorting columns, and has higher efficiency and better consistency of washing finished products compared with manual washing.

Inventors

• TIAN FEI
• LANG LI

Assignees

• 妙顺（上海）生物科技有限公司

Dates

Publication Date

20260505

Application Date

20260408

Claims (10)

1. 1. The magnetic sorting column rinsing device is characterized by comprising a rinsing liquid conveying component, a vacuumizing component, a gas conveying component, a moving component, a sealing cavity (3) and a control unit, wherein the moving component is arranged in the sealing cavity (3) and comprises a sorting column moving component (1) and a sealing plug moving component (2), the sorting column moving component (1) comprises a sorting column holder (11) and a first driving device (12), the sealing plug moving component (2) comprises a sorting column sealing plug (21) and a second driving device (22), the sorting column sealing plug (21) comprises a sealing part (211) and a plurality of channel interfaces (212), the rinsing liquid conveying component and the gas conveying component are connected to the channel interfaces (212) through pipelines, the vacuumizing component is connected to the sealing cavity (3) through pipelines, the first driving device (12) drives the sorting column holder (11) to move, the second driving device (22) drives the sorting column sealing plug (21) to move, and the vacuumizing component, the gas conveying component, the first driving device (12) and the second driving device (22) are connected with the control unit.
2. 2. The magnetic sorting column rinsing device according to claim 1, characterized in that the sorting column holder (11) comprises a connecting section (111) and a holding section (112), that the connecting section (111) is detachably connected to the movable end of the first driving means (12), that the movable end of the second driving means (22) is connected to the sorting column sealing plug (21) by means of a sealing plug holder (23), and that the moving assembly is provided with several groups.
3. 3. The magnetic sorting column rinsing device according to claim 2, characterized in that the second driving means (22) is connected to the movable end of the first driving means (12) by means of a mounting bracket (24), the second driving means (22) being located above the sorting column holder (11).
4. 4. The magnetic sorting column rinsing device according to claim 2, characterized in that the sorting column sealing plug (21) comprises a clamping portion (213), the sealing portion (211) being detachably connected to the clamping portion (213), the clamping portion (213) being shaped to fit the sealing plug holder (23).
5. 5. The magnetic separation column rinse device according to claim 2, characterized in that the gas delivery assembly comprises a gas source, a flow controller and a gas delivery line, the gas source being connected to each separation column sealing plug (21) via the gas delivery line, the gas source and the flow controller being connected to a control unit.
6. 6. The magnetic separation column rinsing device according to claim 2, wherein the rinsing liquid conveying assembly comprises a rinsing liquid bin, a liquid pump and a plurality of rinsing liquid pipelines, and each rinsing liquid pipeline is correspondingly connected with a separation column sealing plug (21).
7. 7. The magnetic separation column rinse device of claim 2, wherein the vacuum assembly comprises a vacuum pump, a vacuum gauge, and a vacuum line, the vacuum pump and the vacuum gauge being connected to a control unit.
8. 8. The magnetic separation column rinsing device according to claim 2, further comprising a residual liquid collecting assembly, wherein the residual liquid collecting assembly comprises a confluence joint (41), a confluence channel (42) and a confluence support (43), a plurality of residual liquid collecting ports (44) are arranged on the confluence support (43) at intervals, the residual liquid collecting ports (44) are in one-to-one correspondence with the moving assembly and are communicated to the confluence channel (42) through the confluence joint (41), and a stop valve connected with the control unit is arranged on the confluence channel (42).
9. 9. A method of washing a magnetic separation column based on the apparatus of any one of claims 1 to 8, comprising: preparing before operation, placing a magnetic separation column to be rinsed in a separation column holder (11), and driving the separation column holder (11) to move downwards by a first driving device (12) so that a liquid outlet at the lower end of the magnetic separation column is inserted into a residual liquid collecting port (44); A rinsing step, wherein a second driving device (22) drives a sorting column sealing plug (21) to move downwards until a sealing part (211) covers an opening of the magnetic sorting column, and a rinsing liquid conveying component is used for introducing rinsing liquid into the magnetic sorting column and stopping after a period of time; a bubble removing step, namely, a second driving device (22) drives a sorting column sealing plug (21) to move upwards so as to separate a sealing part (211) from an opening of the magnetic sorting column, and a vacuum pump is started to vacuumize a sealing cavity (3) to a certain pressure and stopped after a certain time; And removing residues, namely driving the separation column sealing plug (21) to move downwards by the second driving device (22) until the sealing part (211) covers the opening of the magnetic separation column, and enabling the gas conveying assembly to introduce gas into the magnetic separation column through the gas conveying pipe at a certain flow rate for a certain time, and stopping.
10. 10. The method for washing the magnetic separation column according to claim 9, further comprising a drying step after the residue removing step, wherein the second driving device (22) drives the separation column sealing plug (21) to move upwards to separate the sealing part (211) from the opening of the magnetic separation column, the vacuum pump is started to vacuumize the sealing cavity (3), the vacuum pump stops after the pressure in the sealing cavity (3) is not changed any more, and the soaking step is performed once or more after the bubble removing step.

Description

Magnetic separation column rinsing device and method Technical Field The invention relates to the technical field of experimental equipment, in particular to a magnetic separation column rinsing device and method. Background With the development of biotechnology, magnetic separation technology has been widely used in the field of life science, such as cell sorting, protein purification, nucleic acid extraction, and the like. The technology utilizes the magnetic microsphere (magnetic bead) with surface modification to specifically bind with target substances, and then separates the target substances from a complex system through a magnetic field. Magnetic sorting columns are one of the core consumables to achieve this process. At present, the preparation process of the magnetic separation column is very complex, and besides the magnetic filler is filled into the separation column, the magnetic separation column also needs to undergo multiple treatment steps of rinsing, rinsing liquid residue removal, drying and the like. At present, the processing steps are basically finished by manpower, the sample is manually loaded and rinsed, the rinse solution residue is removed by centrifugation through a manual transfer to a centrifugal machine or is removed by ventilation through a ventilation system, and then the rinse solution residue is transferred to a dryer for drying through the manual transfer, so that the efficiency is low, and the consistency among finished magnetic separation column products cannot be ensured. Disclosure of Invention In view of the above-mentioned shortcomings of the existing manual washing separation column, the invention provides a magnetic separation column washing device and method, which can improve the automation degree, the treatment efficiency and the consistency of finished products of the magnetic separation column washing operation. In order to achieve the above purpose, the embodiment of the present invention adopts the following technical scheme: The magnetic sorting column washing device comprises a washing liquid conveying assembly, a vacuumizing assembly, a gas conveying assembly, a moving assembly, a sealing cavity and a control unit, wherein the moving assembly is arranged in the sealing cavity and comprises a sorting column moving assembly and a sealing plug moving assembly, the sorting column moving assembly comprises a sorting column holder and a first driving device, the sealing plug moving assembly comprises a sorting column sealing plug and a second driving device, the sorting column sealing plug comprises a sealing part and a plurality of channel interfaces, the washing liquid conveying assembly and the gas conveying assembly are connected to the channel interfaces through pipelines, the vacuumizing assembly is connected to the sealing cavity through pipelines, the first driving device drives the sorting column holder to move, the second driving device drives the sorting column sealing plug to move, and the vacuumizing assembly, the gas conveying assembly, the first driving device and the second driving device are connected with the control unit. According to one aspect of the invention, the sorting post holder comprises a connecting section and a holding section, the connecting section is detachably connected with the movable end of the first driving device, the movable end of the second driving device is connected with the sorting post sealing plug through a sealing plug holder, and the moving assembly is provided with a plurality of groups. According to one aspect of the invention, the second drive means is connected to the movable end of the first drive means by a mounting bracket, the second drive means being located above the column holder. According to one aspect of the invention, the sorting post sealing plug comprises a clamping portion, the sealing portion is detachably connected with the clamping portion, and the shape of the clamping portion is matched with that of the sealing plug retainer. According to one aspect of the invention, the gas delivery assembly includes a gas source, a flow controller and a gas delivery line, the gas source being connected to each of the sorting column sealing plugs through the gas delivery line, the gas source and the flow controller being connected to a control unit. According to one aspect of the invention, the rinse solution delivery assembly includes a rinse solution cartridge, a liquid pump, and a plurality of rinse solution lines, each of which is correspondingly connected to a column seal plug. According to one aspect of the invention, the vacuum assembly comprises a vacuum pump, a vacuum gauge and a vacuum pumping pipeline, wherein the vacuum pump and the vacuum gauge are connected with a control unit. According to one aspect of the invention, the device further comprises a residual liquid collecting assembly, wherein the residual liquid collecting assembly comprises a confluence joint, a confluence channel and a c