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CN-121974312-A - Modified hydroxyapatite for adsorbing dust mite allergen, synthesis method and application

CN121974312ACN 121974312 ACN121974312 ACN 121974312ACN-121974312-A

Abstract

The invention provides modified hydroxyapatite for adsorbing dust mite allergens, a synthesis method and application thereof. The dust mite allergen in the air mainly exists in a protein form, and human body is exposed to the environment to be passively inhaled or contacted with the dust mite allergen, so that diseases such as allergic rhinitis, asthma and the like are induced. The modified hydroxyapatite has a unique porous crystal structure, wherein the content of the main component Ca 10 (PO 4 ) 6 (OH) 2 is more than or equal to 99: 99 wt%, the surface is rich in hydroxyl (-OH), and functional groups such as amino (-NH 2 ) and carboxyl (-COOH) can be introduced through targeted modification, so that the modified hydroxyapatite is in a flaky or rod-shaped crystal form. The method removes dust mite allergens in the air through the synergistic effect of physical adsorption and chemical adsorption, and improves the removal rate of the dust mite allergens Der p1 and Der f1 under the condition of room temperature. The invention also discloses a synthesis method of the modified hydroxyapatite and application of the modified hydroxyapatite in the field of air purification, and provides a high-efficiency solution for indoor allergen prevention and control.

Inventors

  • YANG FANGXING
  • CHEN JIANAN

Assignees

  • 浙江大学长三角智慧绿洲创新中心

Dates

Publication Date
20260505
Application Date
20251229

Claims (7)

  1. 1. A method for synthesizing modified hydroxyapatite for adsorbing dust mite allergens, comprising the steps of: (1) Preparing a precursor, namely preparing a solution by selecting a calcium source and a phosphorus source, wherein the molar ratio of calcium ions to phosphorus ions is 1:1-1.8:1, dropwise adding the phosphorus source into the calcium source solution at the rate of 1 drop/s, adjusting the pH value to 9-11 by using 0.1 mol/L of sodium hydroxide solution, and magnetically stirring to form uniform slurry; (2) Hydrothermal synthesis, namely transferring the slurry to a high-pressure reaction kettle, and carrying out hydrothermal reaction for 6-24 hours at 80-160 ℃; (3) Post-treatment, namely washing and centrifuging a reaction product, vacuum drying at 40-120 ℃ for 6-12 hours, grinding and sieving to obtain powder; (4) The modification comprises the steps of mixing the powder obtained in the step (3) with a surface modifier, carrying out ultrasonic treatment at a constant temperature of between 60 and 80 ℃ for 1 to 4 hours to enable the modifier to be fully grafted on the surface of a material, centrifugally separating and precipitating, washing the precipitate with deionized water until the washing liquid is colorless, carrying out vacuum drying at 40 to 120 ℃ for 6 to 12 hours, grinding and sieving to obtain the modified hydroxyapatite, wherein the addition amount of the surface modifier is 1 to 10 percent of the mass of the powder.
  2. 2. The method according to claim 1, wherein in the step (1), the calcium source is one or more of calcium nitrate, calcium chloride, calcium carbonate, calcium hydroxide and calcium acetate, and the phosphorus source is one or more of diammonium hydrogen phosphate, monoammonium phosphate, disodium hydrogen phosphate and phosphoric acid.
  3. 3. The method of claim 1, wherein in step (4), the surface modifier is selected according to the functional group, wherein the functional group is one or more of amino, carboxyl, and hydroxyl.
  4. 4. A modified hydroxyapatite prepared by the synthetic method of any one of claims 1 to 3.
  5. 5. Use of modified hydroxyapatite according to claim 4 in air purification, in particular for adsorbing dust mite allergens in air.
  6. 6. The use according to claim 5, wherein the dust mite allergens comprise dust mite allergens Der p1, der f 1.
  7. 7. An air purification filter material comprising the modified hydroxyapatite according to claim 4.

Description

Modified hydroxyapatite for adsorbing dust mite allergen, synthesis method and application Technical Field The invention belongs to the field of risk control of environmental health, and particularly relates to modified hydroxyapatite for adsorbing dust mite allergens, a synthesis method and application thereof. Background When the allergen protein particles are inhaled through the respiratory tract or contact with the skin, they enter the human body, they are recognized and processed by antigen presenting cells in the immune system, and then activate T lymphocytes to differentiate into Th2 cells. Th2 cells secrete cytokines such as interleukin-4 and interleukin-5, on one hand induce B lymphocytes to proliferate and differentiate into plasma cells and produce specific IgE antibodies, which can be combined with Fc receptors on the surfaces of mast cells and basophils to make organisms in a sensitized state for the allergens, and on the other hand promote eosinophils to activate and recruit to target organs such as respiratory tracts, skin and the like. When the organism contacts the same allergen again, the allergen can be combined with IgE antibody on the surface of sensitized cells specifically, trigger cell degranulation reaction, release biological active mediums such as histamine, leukotriene and the like, and the mediums rapidly act on blood vessels, smooth muscles and glands to trigger a series of pathological reactions such as vasodilation, permeability increase, smooth muscle contraction and the like, and finally the allergic symptoms are presented. In the field of environmental health risk control, indoor air dust mite allergen exposure has become one of the core risk factors for inducing allergic diseases. Up to now, 44 allergens from Der f and 40 allergens from Der p have been named by the world health organization/international immunology association allergen naming group committee. Among them, group I allergens of dust mites (including Der p 1 and Der f 1) are the most important allergens responsible for allergic diseases in indoor environments, and both have 81% sequence identity and antigen cross-reactivity. The isoelectric points of Der p 1 and Der f1 are about 5.65 and 5.66 respectively, the relative molecular mass is about 24kDa and 27kDa respectively, the particle size is tiny, and the Der p 1 and the Der f1 can suspend indoors for a long time and form serious health threat to the old, children and allergic constitution people. Most of the allergic attacks are directly related to indoor dust mite allergen exposure, so that the accurate prevention and control of indoor dust mite allergens has become an important research point in the field of environmental health. In summary, the health hazard caused by indoor dust mite allergens is quite clear and forms a significant threat to human beings, and a short board exists in the precise prevention and control technology for free dust mite allergen proteins in the air, so that the development of efficient and safe targeted removal technology is needed to fill the gap. The existing dust mite allergen control technology mainly surrounds physical removal, symptom relief and air purification expansion, has obvious technical limitations that physical removal means such as dust collector cleaning, fabric airing and the like can only remove dust mite allergens attached to the surface of an object, has little effect on protein particles suspended in the air and is easy to cause secondary dust emission, and drug treatment can only relieve allergic symptoms and can not block the exposure of the dust mite allergens from the source. The traditional air purifying material mainly adopts the technologies of adsorption, mechanical filtration, electrostatic dust removal, negative ions, plasma method and the like to collect and filter suspended particles in the air. These techniques can remove proteins attached to particulate matter to some extent, but have weak adsorption targeting of free dust mite allergen proteins and low removal efficiency. Disclosure of Invention Aiming at the defect that the prior art can not effectively reduce the pathogenic risk of free dust mite allergen protein in indoor air, the invention provides a modified hydroxyapatite for adsorbing dust mite allergen, a synthesis method and application thereof, the hydroxyapatite has a porous needle-like/short rod-like crystal structure, the surface is rich in hydroxyl and modifiable functional groups, the novel co-precipitation-hydrothermal-grafting method is used for synthesizing the dust mite allergen protein in indoor air, is suitable for carriers such as an air purifier filter screen and a fresh air system, reduces allergy risks from the source, and fills a short plate in the aspect of accurate removal of free dust mite allergen in the prior art. The invention aims at realizing the following technical scheme: In a first aspect, the present invention provides a method of synthesizing a modifie