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CN-121974332-A - All-weather broad-spectrum antibacterial and antivirus carbon nano-dot, and preparation method and application thereof

CN121974332ACN 121974332 ACN121974332 ACN 121974332ACN-121974332-A

Abstract

The invention relates to the technical field of nano antibacterial materials. More particularly, relates to an all-weather broad-spectrum antibacterial and antivirus carbon nano-dot, and a preparation method and application thereof. The preparation method of the all-weather broad-spectrum antibacterial and antivirus carbon nano-dot comprises the following steps of 1) adding an auxiliary agent solution into a high polymer to obtain a precursor solution, 2) transferring the precursor solution into a reactor to react for 1-24 hours at 100-500 ℃, 3) cooling to room temperature after the reaction is finished, collecting a reaction solution, and separating and purifying to obtain the carbon nano-dot, wherein the high polymer is selected from the following structures. The carbon nano dot can show high-efficiency killing effect on pathogenic microorganisms including but not limited to bacteria such as escherichia coli, staphylococcus aureus, candida albicans, fungi, influenza viruses and the like in all-weather environments such as sunny days, overcast days, daytime, night and the like, and meanwhile, the carbon nano dot prepared by the method is high in biosafety and high in cost performance.

Inventors

  • GE JIECHAO
  • HAO YONGLIANG
  • CHEN TIEJIN
  • ZHANG TIERUI
  • ZHOU CHAO

Assignees

  • 中国科学院理化技术研究所

Dates

Publication Date
20260505
Application Date
20260127

Claims (10)

  1. 1. The preparation method of the all-weather broad-spectrum antibacterial and antivirus carbon nano-dot is characterized by comprising the following steps of: 1) Adding an auxiliary agent solution into a high-molecular polymer to obtain a precursor solution; 2) Transferring the precursor solution into a reactor, and reacting for 1-24 hours at 100-500 ℃; 3) Cooling to room temperature after the reaction is finished, collecting a reaction liquid, and separating and purifying to obtain the carbon nano-dots; Wherein the high molecular polymer is selected from the following structures: ; m and n are natural numbers of 0-10000, and m and n are not 0 at the same time; Ar 1 is selected from benzene, naphthalene, anthracene, pyrene, indole, thiophene, carbazole, rhodamine, fluorene, quinoline, cyano dye, pyrrole, coumarin, fluorescein, or carbazole; X, Y are each independently selected from O, S, se, N or Si; z, R 1 、R 2 、R 3 、R 4 are each independently selected from hydrogen, alkyl groups having 1 to 18 carbon atoms, hydroxyl groups, mercapto groups, carboxyl groups, amino groups, amide groups, cyano groups, alkenyl groups, alkynyl groups, aryl groups, ester groups, ether groups, quaternary ammonium salts, sulfonate groups, phosphate groups, or polyethylene glycol groups.
  2. 2. The method of claim 1, wherein the high molecular polymer is selected from the structures shown below: 1) PT1, n is an integer of 3-3000; 2) CPT1, m is an integer from 3 to 3000, n is an integer from 3 to 750; 3) CPT2, m is an integer from 3 to 1000, n is an integer from 3 to 5000; 4) PT2 and n are integers of 3-3000.
  3. 3. The method according to claim 1, wherein the high molecular polymer has a number average molecular weight of 10 4 -10 5 Da and a light absorption range of 400-800nm.
  4. 4. The method according to claim 1, wherein the auxiliary agent is one selected from the group consisting of an acidic compound, a basic compound and a neutral compound.
  5. 5. The preparation method according to claim 1, wherein the auxiliary agent is one or more selected from the group consisting of hydrochloric acid, phosphoric acid, sodium hydroxide, potassium hydroxide, ammonia water, sodium chloride, potassium chloride and sodium bisulfate.
  6. 6. The method of claim 1, wherein the concentration of the high molecular weight polymer in the precursor solution is 5-1000 ug/mL.
  7. 7. The method of claim 1, wherein the concentration of the adjuvant solution is 0.1-1M.
  8. 8. An all-weather broad-spectrum antibacterial and antivirus carbon nanodot, which is characterized by being prepared by the preparation method of any one of claims 1-7.
  9. 9. The all-weather broad spectrum antibacterial carbon nanodot of claim 8, wherein the carbon nanodot has a particle size of 2-50 nm.
  10. 10. Use of the all-weather broad-spectrum antimicrobial carbon nanodot according to claim 8 or 9 for killing harmful pathogenic microorganisms under light or in the absence of light, wherein the use further comprises: And (3) adding the all-weather broad-spectrum antibacterial and antivirus carbon nanodot solution into a system containing bacteria, fungi or viruses, and optionally carrying out irradiation with visible light or light-shielding culture to complete the killing of the bacteria, fungi or viruses, wherein the concentration of the all-weather broad-spectrum antibacterial and antivirus carbon nanodot solution is more than 100 ug/mL.

Description

All-weather broad-spectrum antibacterial and antivirus carbon nano-dot, and preparation method and application thereof Technical Field The invention relates to the technical field of nano antibacterial materials. More particularly, relates to an all-weather broad-spectrum antibacterial and antivirus carbon nano-dot, and a preparation method and application thereof. Background Infectious diseases caused by pathogenic microorganisms (bacteria, fungi, viruses and the like) are always a great threat in the field of global public health, and with excessive abuse of antibiotics, drug-resistant strains (especially gram-negative drug-resistant bacteria) are largely emerging, so that the health and survival safety of human beings are seriously threatened. Meanwhile, the requirements for efficient and safe antibacterial and antivirus materials are increasingly urgent in various fields of medical equipment sterilization, food processing and fresh-keeping, water treatment and purification, public environment protection and the like. The existing antibacterial materials are mainly divided into two major classes of traditional chemical antibacterial agents and novel nano antibacterial materials. The traditional chemical antibacterial agents such as quaternary ammonium salts and silver ions have the remarkable defects that quaternary ammonium salt antibacterial materials are easy to be quickly adapted by bacteria to generate drug resistance, and the silver ion antibacterial materials can cause toxic and side effects on human bodies after long-term use and have potential ecological risks. The novel nano antibacterial material becomes a research hot spot in the antibacterial field due to unique physical and chemical properties, wherein the carbon nano material (such as graphene quantum dots, carbon nano tubes and the like) has wide application prospect by virtue of strong antibacterial activity, low drug resistance tendency and good biocompatibility. However, most of the existing carbon-based nano antibacterial materials depend on photocatalysis (sensitization), photo-thermal and other conditions to activate antibacterial activity, so that the antibacterial efficiency is obviously reduced in the environment of insufficient natural light or indoor illumination, the antibacterial capability is almost lost in the dark environment, the application of the carbon-based nano antibacterial materials in non-illumination scenes (such as underground space, closed pipelines and night protection) is severely limited, and the carbon-based nano antibacterial materials cannot play a role in all-weather environments. In summary, developing a carbon nano dot material with good biocompatibility, high cost performance and all-weather broad-spectrum antibacterial property becomes a key technical problem to be solved urgently in the field of the current antibacterial materials, and has important significance for promoting the clinical transformation and the industrialized application of the antibacterial materials. Disclosure of Invention Based on the defects, the first aim of the invention is to provide a preparation method of all-weather broad-spectrum antibacterial and antivirus carbon nano-dots. The second aim of the invention is to provide the all-weather broad-spectrum antibacterial and antivirus carbon nano-dot prepared by the preparation method. The carbon nanodots can kill bacteria, fungi, viruses and the like under the irradiation of visible light (including but not limited to sunlight and lamplight) or in darkness (under the light-shielding condition), and can realize all-weather broad-spectrum antibacterial effect. A third object of the present invention is to provide an application of the above-mentioned all-weather broad-spectrum antibacterial and antivirus carbon nanodots in killing harmful pathogenic microorganisms under illumination or in the dark. In order to achieve the first object, the present invention adopts the following technical scheme: the invention discloses a preparation method of all-weather broad-spectrum antibacterial and antivirus carbon nanodots, which comprises the following steps: 1) Adding an auxiliary agent solution into a high-molecular polymer to obtain a precursor solution; 2) Transferring the precursor solution into a reactor, and reacting for 1-24 hours at 100-500 ℃; 3) Cooling to room temperature after the reaction is finished, collecting a reaction liquid, and separating and purifying to obtain the carbon nano-dots; Wherein the high molecular polymer is selected from the following structures: ; m and n are natural numbers of 0-10000, and m and n are not 0 at the same time; Ar 1 is selected from benzene, naphthalene, anthracene, pyrene, indole, thiophene, carbazole, rhodamine, fluorene, quinoline, cyano dye, pyrrole, coumarin, fluorescein, or carbazole; X, Y are each independently selected from O, S, se, N or Si; z, R 1、R2、R3、R4 are each independently selected from hydrogen, alkyl groups having 1 to 18