CN-121974333-A - Janus carbon dot with bidirectional active oxygen regulation function and preparation method and application thereof

CN121974333ACN 121974333 ACN121974333 ACN 121974333ACN-121974333-A

Abstract

The invention provides a Janus carbon dot with a bidirectional active oxygen regulation function, and a preparation method and application thereof, belonging to the technical field of nano medicine, immune regulation and anti-infection treatment intersection. The Janus carbon dot is prepared by taking plant raw materials as precursors through a hydrothermal method. The preparation method comprises mixing plant materials with water, performing hydrothermal reaction, cooling the reaction solution, centrifuging, filtering, purifying and drying to obtain the Janus carbon dots. The Janus carbon dot is applied to the preparation of medicines for treating bacterial infectious diseases. The Janus carbon dot prepared by the method realizes the ROS bidirectional dynamic switching based on the carbon dot for the first time, responds to the micro-environment change, and has the advantage of intelligent bidirectional regulation. In addition, the Janus carbon dot prepared by the invention can realize immune cell targeting, so that macrophages can selectively endocytose, and the treatment accuracy and the biological safety are improved. The preparation method of the invention takes plants as raw materials, is environment-friendly, has simple synthesis process and is easy for large-scale production and application.

Inventors

LOU QING
ZHENG GUANGSONG
SHEN CHENGLONG
SHAN CHONGXIN

Assignees

郑州大学

Dates

Publication Date: 20260505
Application Date: 20260204

Claims (9)

1. The Janus carbon dot with the bidirectional active oxygen regulation function is characterized in that the Janus carbon dot is prepared by taking plant raw materials as precursors through a hydrothermal method.
2. The Janus carbon dot with bi-directional active oxygen regulating function according to claim 1, wherein the plant material comprises one of spinach, black nightshade or coptis.
3. The Janus carbon dot with bi-directional active oxygen regulating function according to claim 1, wherein the average particle size of the Janus carbon dot is 1.5-2.5 nm; the Janus carbon point has a nitrogen/oxygen co-doping structure, the surface is rich in oxygen and nitrogen-containing functional groups, active oxygen is generated under the illumination condition, and the active oxygen is removed under the non-illumination condition.
4. The Janus carbon dot with bi-directional reactive oxygen species regulation function of claim 3, wherein the Janus carbon dot generates singlet oxygen by type II energy transfer under light of wavelength 660 nm; The Janus carbon dots scavenge H 2 O 2 、•O 2- ,. OH, and 1 O 2 in the absence of light.
5. A method for preparing the Janus carbon dot with bi-directional active oxygen controlling function according to any one of claims 1 to 4, comprising the steps of: and mixing plant raw materials with water, performing hydrothermal reaction, and cooling, centrifuging, filtering, purifying and drying the obtained reaction liquid to obtain the Janus carbon dots.
6. The method for preparing Janus carbon dots with bidirectional active oxygen regulation and control function according to claim 5, wherein the temperature of the hydrothermal reaction is 100-140 ℃ and the time is 5-30 min; the cooling temperature is 0-20 ℃ and the cooling time is 30-60 min; the rotational speed of the centrifugation is 4000-8000 rpm and the time is 5 min; the filtering is carried out by adopting a filter membrane with the diameter of 0.22 mu m; the drying is freeze drying, the drying temperature is-50 ℃ and the time is 24 h, and the sample is freeze-dried to be powder.
7. Use of a Janus carbon dot having bi-directional active oxygen modulation function according to any one of claims 1-4 for the manufacture of a medicament for the treatment of bacterial infectious diseases.
8. The use according to claim 7, wherein the bacterial infectious disease comprises a soft tissue infection of the skin, a diabetic foot ulcer, an implant-related infection or chronic osteomyelitis.
9. A medicament for treating bacterial infectious diseases, which contains the Janus carbon dots with bi-directional active oxygen regulating function according to any one of claims 1 to 4.

Description

Janus carbon dot with bidirectional active oxygen regulation function and preparation method and application thereof Technical Field The invention belongs to the technical field of intersection of nano medicine, immune regulation and anti-infection treatment, and particularly relates to a Janus carbon dot with a bidirectional active oxygen regulation function, and a preparation method and application thereof. Background Bacterial infection is a serious health threat to be solved in the global scope, especially in recent years, the spreading trend of drug-resistant bacteria is increasingly severe, so that the treatment effect of traditional antibiotics is continuously attenuated, even the dilemma that multi-drug-resistant bacteria infection can be treated without drugs occurs, and the bacterial infection brings great challenges to clinical anti-infection treatment and also causes heavy social medical burden. In the immune defense mechanism of organisms, reactive Oxygen Species (ROS) play a double key role in that, on one hand, a proper amount of ROS can be used as a powerful antibacterial molecule to directly kill pathogens by destroying the integrity of bacterial cell membranes, oxidatively damaging nucleic acids, proteins and the like, and on the other hand, if excessive ROS are generated at an infected part, severe oxidative stress reaction can be induced, so that normal tissue cells of a host can be damaged, the immune function of the organisms can be weakened, inflammatory reaction can be aggravated, and healing processes of infected wound surfaces and the like can be seriously hindered. Therefore, the development of the difunctional regulator capable of accurately responding to the change of the infection microenvironment and intelligently switching the functions of eliminating ROS and generating ROS realizes the dynamic and accurate regulation of ROS level, and becomes an innovative strategy for breaking through the bottleneck of traditional anti-infection treatment. However, the existing ROS regulation and control materials have the inherent defect of single function, that most materials only can generate active oxygen (such as a photosensitizer in photodynamic therapy) or remove active oxygen (such as a traditional antioxidant) in one way, bidirectional dynamic regulation and control cannot be realized according to the actual microenvironment requirement of an infection part, and the space-time accurate immunoregulation effect is difficult to achieve, so that the application efficiency of the ROS regulation and control materials in anti-infection treatment is limited. It is worth noting that the evolution of plant systems in nature forms a high-efficiency ROS balance network, and can rapidly generate reactive oxygen species to resist infection when encountering pathogen attack, and simultaneously timely remove excessive ROS through a self antioxidant system, so that oxidative damage is avoided, and dynamic balance of growth and defense is realized. The subtle bionic regulation mechanism provides valuable design inspiration for developing novel ROS regulation materials. However, up to now, the research and development of ROS regulation and control materials for anti-infection immunotherapy by applying the bionic design concept of a plant-derived ROS balance network is still in a blank state, and related technical reports are not yet seen. Janus carbon dots (J-CDs) are used as novel carbon nano materials with surface space asymmetric structures, and the unique double-sided heterostructure of the novel carbon nano materials can realize partition loading and synergistic effect of different functional groups, so that an ideal structural foundation is provided for constructing a multifunctional integrated ROS regulation system. In the prior art, janus carbon dots have demonstrated application potential in the fields of interface stability, catalysis and the like, and part of carbon dot materials are proved to have single ROS generation or removal capability, but the structural advantages of Janus carbon dots are not researched to be combined with bidirectional ROS regulation and control functions, and the bionic design thought of a plant ROS balance network is not introduced, so that the core requirement on ROS dynamic and accurate regulation and control in anti-infection treatment cannot be met. Based on the state of the art, a Janus carbon dot with bionic design characteristics and bidirectional active oxygen regulation and control functions is developed, and has important practical significance and clinical application value for solving the problem of drug-resistant bacteria infection treatment and improving the anti-infection treatment effect. Disclosure of Invention In order to solve the problems in the prior art, the invention provides a Janus carbon dot with a bidirectional reactive oxygen species regulation function, a preparation method and application thereof, and the Janus carbon dot can intell