CN-121974453-A - Antibacterial method for low-field-intensity ultrasonic coupling cyclohexanol

Abstract

The invention discloses a bacteriostasis method of low-field-intensity ultrasonic coupling cyclohexanol, belonging to the technical field of industrial water system microorganism control and biofilm inhibition. The method comprises the steps of adding cyclohexanol into a water system to be treated to enable the final concentration of the cyclohexanol to be 300-350 mu g/mL, and then applying probe type ultrasonic treatment for 5-30min under the field intensity of 15.79-21.05W/cm 2 . In the process window, low-field intensity ultrasound can remarkably improve the transmembrane delivery and intracellular effective enrichment efficiency of cyclohexanol, so that stable synergistic bacteriostasis is realized in a subinhibition concentration range, biofilm formation and regeneration are inhibited, the blockage caused by microbial pollution and the risk of microbial induced corrosion are reduced, the maintenance frequency is reduced, and the service life of equipment is prolonged.

Inventors

• YU HANG
• WANG MENGRU
• LI HUANHUAN
• CHEN MENG
• YUAN SHAOFENG
• DU YUHANG
• YAO WEIRONG

Assignees

• 江南大学

Dates

Publication Date

20260505

Application Date

20260318

Claims (10)

1. 1. A method of inhibiting microorganisms in a cooling water, reuse water and heat exchange device medium circulating water system, the method comprising: And adding cyclohexanol into the water system to ensure that the final concentration of the cyclohexanol is 300-350 mug/mL, and performing ultrasonic treatment under the ultrasonic field intensity of 15.79-21.05W/cm 2 for 5-30 min.
2. 2. The method of claim 1, wherein the ultrasound has a frequency of 18 to 25 kHz and a sonication temperature of 20 to 40 ℃.
3. 3. The method of claim 1, wherein the ultrasound is probe ultrasound and the ultrasound probe is 2-6 cm a distance from the liquid surface.
4. 4. The method of claim 1, wherein the water system is an industrial circulating cooling water system, an industrial reuse water system, a central air conditioning cooling water system, or a heat exchange device medium circulating water system.
5. 5. The method of claim 1, wherein the microorganism is a pseudomonas microorganism.
6. 6. The method according to any one of claims 1 to 5 for inhibiting microorganisms in an industrial circulating cooling water system, an industrial reuse water system, a central air conditioning cooling water system or a medium circulating water system of heat exchange equipment.
7. 7. A method for increasing the bacteriostatic rate of cyclohexanol, said method comprising: Adding cyclohexanol into water system to make the final concentration of cyclohexanol be 300-350 mug/mL, and making ultrasonic treatment under the ultrasonic field intensity of 15.79-21.05W/cm 2 to make 5-30 min.
8. 8. The method of claim 7, wherein the ultrasound has a frequency of 18-25 kHz and a sonication temperature of 20-40 ℃, the ultrasound is probe ultrasound, and the distance of the ultrasound probe from the liquid surface is 2-6 cm.
9. 9. The method of claim 7, wherein the water system is an industrial circulating cooling water system, an industrial reuse water system, a central air conditioning cooling water system, or a heat exchange device medium circulating water system.
10. 10. The method of claim 7, wherein the microorganism is a pseudomonas microorganism.

Description

Antibacterial method for low-field-intensity ultrasonic coupling cyclohexanol Technical Field The invention relates to a bacteriostasis method of low-field-intensity ultrasonic coupling cyclohexanol, belonging to the technical field of industrial water system microorganism control and biofilm inhibition. Background Industrial circulating cooling water/reuse water and heat exchange equipment medium circulating water systems are in an operation environment which is rich in nutrition, moist and accompanied by continuous fluid shearing for a long time, and microorganisms represented by pseudomonas and the like are easy to grow, and a biological film with a complex structure and firm adhesion is formed. Once the biomembrane is formed, the heat exchange efficiency is reduced, the energy consumption of the system is increased, the pipeline and the heat exchanger are possibly blocked, the running resistance is increased, and meanwhile, the material degradation of equipment is accelerated by the induced corrosion of microorganisms, so that the maintenance frequency is increased, and the service life of the equipment is shortened. At present, the engineering practice is often controlled by adopting measures such as oxidizing or non-oxidizing biocides, surfactants, alcohol or amine bacteriostats and the like. However, the chemical method often requires higher dosage and higher dosing frequency, which causes the increase of medicament cost, and possibly brings material compatibility and emission treatment pressure, and in addition, long-term high-dosage chemical intervention can induce the enhancement of microbial tolerance, so that biological films are regenerated and repeatedly accumulated, and the treatment effect is difficult to last. The ultrasonic wave is used as a physical strengthening means capable of being engineered, has cavitation, micro-jet flow, mechanical shearing and other effects, and can generate disturbance to microorganisms and biological membranes. However, the independent ultrasonic treatment still can be difficult to consider the energy consumption and the effect stability, and meanwhile, the effective delivery efficiency of the micromolecular bacteriostatic agent in a water system is influenced by factors such as volatility, dispersibility, film penetrating efficiency and the like, so that the bacteriostatic effect is fluctuated, and the dosage window is difficult to detect. Therefore, a bacteriostasis strategy of 'physical-chemical synergy' is needed, and the stable and amplified bacteriostasis effect is obtained under the conditions of lower energy consumption and lower addition amount by improving the transmembrane delivery and intracellular effective enrichment of small molecules in a mild process window. Disclosure of Invention In order to solve the problems, the invention provides a bacteriostasis method for low-field-intensity ultrasonic coupling cyclohexanol. The method is suitable for controlling the microorganisms and inhibiting the biological membranes of the water system of the non-direct contact products such as industrial circulating cooling water, reuse water, medium circulating water systems of heat exchange equipment and the like. The cyclohexanol is added in a subinhibition concentration range and low field intensity ultrasonic treatment is applied, so that the antibacterial efficiency of the cyclohexanol can be remarkably improved, and the purposes of reducing the adding amount of the cyclohexanol, inhibiting the formation and regeneration of a biological film on the surface of equipment, reducing the blockage and corrosion risks caused by microbial contamination and prolonging the service life of the equipment are realized. A first object of the present invention is to provide a method for inhibiting microorganisms in a circulating medium water system of cooling water, reuse water and heat exchange equipment, the method comprising: Cyclohexanol with a final concentration of 300-350 mug/mL is added into the water system, and ultrasonic wave is conducted under the field intensity of 15.79-21.05W/cm 2 for 5-30 min. In one embodiment, the frequency of the ultrasound is 18-25 kHz and the temperature of the ultrasound is 20-40 ℃. In one embodiment, the ultrasound is probe ultrasound, and the distance between the ultrasound probe and the liquid level is 2-6 cm. In one embodiment, the water system is an industrial circulating cooling water system, an industrial reuse water system, a central air conditioning cooling water system, or an intermediate circulating water system in a heat exchange device. In one embodiment, the microorganism is a pseudomonas microorganism. A second object of the present invention is to provide the use of any of the above methods for inhibiting microorganisms in an industrial circulating cooling water system, an industrial reuse water system, a central air conditioning cooling water system or a medium circulating water system in heat exchange equipment. The