CN-121974481-A - Biological affinity immobilized microorganism carrier and preparation method and application thereof

Abstract

The invention discloses a biological affinity immobilized microorganism carrier and a preparation method and application thereof, comprising the following steps of cutting a polyurethane substrate into a specific shape and cleaning; loading ferroferric oxide nano particles on the surface of the cleaned substrate, uniformly adhering the ferroferric oxide nano particles through ultrasonic dispersion and magnetic stirring, drying the ferroferric oxide nano particles, and carrying out cationic polyacrylamide modification treatment on the carrier to further optimize the performance of the carrier. The carrier prepared by the method improves the roughness and specific surface area of the carrier, and has good hydrophilic and biological properties. The biological affinity immobilized microorganism carrier is applied to the moving bed biological film reactor, so that the biological film forming efficiency and biomass can be greatly improved, the sewage removing effect is enhanced, and a new strategy is provided for the efficient operation of the moving bed biological film reactor.

Inventors

• BAI XUE
• HU HAN
• LIU XIAOFENG

Assignees

• 河海大学

Dates

Publication Date

20260505

Application Date

20251226

Claims (10)

1. 1. A preparation method of a biocompatible immobilized microbial carrier is characterized by comprising the following steps: S1, cutting polyurethane into a specific shape to obtain a carrier with the specific shape; s2, sequentially soaking the carrier with the specific shape obtained in the step S1 in absolute ethyl alcohol and ultrapure water, and cleaning under ultrasonic conditions to remove surface impurities to obtain a cleaned carrier; s3, adding the ferroferric oxide nano particles into absolute ethyl alcohol, and performing ultrasonic treatment and dispersion to obtain a dispersion solution; s4, immersing the washed cube carrier obtained in the step S2 into the dispersion solution obtained in the step S3, magnetically stirring at room temperature, washing with deionized water after drying, and drying in a blast drying oven to obtain a magnetic carrier; s5, soaking the magnetic carrier obtained in the step S4 in a cationic polyacrylamide solution, stirring, and drying by blowing to obtain a modified carrier; S6, washing and drying the modified carrier obtained in the step S5 again to obtain the biological affinity immobilized microorganism carrier.
2. 2. The method of claim 1, wherein the polyurethane in step S1 is cut into cubes with a side length of 1.0-1.8 cm.
3. 3. The method according to claim 1, wherein the cleaning conditions in the step S2 are that 1-3 h is cleaned under 40-80 kHz ultrasonic.
4. 4. The method of claim 1, wherein the mass of the ferroferric oxide nanoparticles in the step S3 is 500-700 mg, and the volume of the absolute ethyl alcohol is 180-220 mL.
5. 5. The preparation method of the ceramic material according to claim 1, wherein the cube carrier cleaned in the step S4 is immersed in the dispersion solution for magnetic stirring for 10-12 hours at a stirring speed of 150-250 rpm, and is dried for 4-8 hours at a blast drying condition of 60 ℃.
6. 6. The method of claim 1, wherein the concentration of the cationic polyacrylamide solution in the step S5 is 3 g/L.
7. 7. The method according to claim 1, wherein in the step S5, the stirring time is 2-4 hours, and the stirring speed is 150-250 rpm; and drying for 4-8 hours at the temperature of 75 ℃ under the condition of forced air drying.
8. 8. A biocompatible immobilized microorganism carrier produced by the production method according to claim 1.
9. 9. Use of the bioaffinity immobilized microorganism carrier of claim 8 in wastewater treatment.
10. 10. The method according to claim 9, wherein the waste water is an antibiotic-containing waste water.

Description

Biological affinity immobilized microorganism carrier and preparation method and application thereof Technical Field The invention belongs to the technical field of sewage treatment, and particularly relates to a biological affinity immobilized microorganism carrier, a preparation method and application thereof. Background With the deep development of national energy conservation and emission reduction strategies, the increasingly stricter environmental protection policies and the continuous improvement of emission standards, the difficulty of sewage treatment is continuously increased, more advanced and stable technology and more reliable materials are urgently needed in the field of sewage treatment, and the improvement of the performance of the biological carrier serving as a key material is particularly important. The moving bed biological film reactor is a novel and efficient biological treatment process formed by combining a traditional activated sludge method and a biological contact oxidation method, and has the core that the material and the surface property of a biological carrier play a key role in the formation of a biological film. However, the types of biovectors or fillers currently on the market include mainly polyhedral balls, loaded fiber balls, elastic fillers, combined fillers, polyester network fillers, and the like. These conventional carriers have various disadvantages in practical applications, such as poor hydrophilicity, poor temperature resistance, easy aging, easy abrasion, short service life, etc., and have become a key problem limiting their wide industrial application. Some studies and patents have provided improvements to this problem, but at the same time have some problems. For example, chinese patent CN109734180a and CN105175591a respectively propose to prepare polyvinyl formal sponge or porous composite filler by carbonate foaming agent to improve hydrophilicity and mechanical properties, but in actual production process, such carbonate foaming agent has defects of complex process production, uneven cell size distribution, and most of products being small-pore dense materials, which limits further application in the field of biological carriers. The biological carrier material mainly prepared from polyvinyl alcohol and nano sepiolite provided in the Chinese patent CN115418066A has obviously improved hydrophilicity, water absorption performance, tensile strength and tearing strength, but has poorer stability and durability in the actual treatment of wastewater. The carrier prepared by mixing the iron-sulfur-based powder with the plastic particles in CN119161014A shortens the film forming time, strengthens the biological denitrification effect, saves a great deal of cost, but has complex subsequent treatment and can generate potential risks to the environment. Based on the problems in the prior art, the invention provides a preparation method and application of an optimized modified biological affinity immobilized microorganism carrier. Disclosure of Invention Aiming at the technical problems, the invention provides the bioaffinity immobilized microorganism carrier, the preparation method and the application thereof, and the carrier not only remarkably improves the surface hydrophilicity and microorganism adhesion capability, but also shows excellent performance in the aspects of wear resistance, stability, service life and the like by loading ferroferric oxide nano particles on the surface of polyurethane and further introducing cationic polyacrylamide for modification, so that the key problems in the prior art can be effectively solved. The invention provides a preparation method of a biocompatible immobilized microorganism carrier, which comprises the following steps: S1, cutting polyurethane into a specific shape to obtain a carrier with the specific shape; s2, sequentially soaking the carrier with the specific shape obtained in the step S1 in absolute ethyl alcohol and ultrapure water, and cleaning under ultrasonic conditions to remove surface impurities to obtain a cleaned carrier; s3, adding the ferroferric oxide nano particles into absolute ethyl alcohol, and performing ultrasonic treatment and dispersion to obtain a dispersion solution; s4, immersing the washed cube carrier obtained in the step S2 into the dispersion solution obtained in the step S3, magnetically stirring at room temperature, washing with deionized water after drying, and drying in a blast drying oven to obtain a magnetic carrier; s5, soaking the magnetic carrier obtained in the step S4 in a cationic polyacrylamide solution, stirring, and drying by blowing to obtain a modified carrier; S6, washing and drying the modified carrier obtained in the step S5 again to obtain the biological affinity immobilized microorganism carrier. Preferably, in the step S1, the polyurethane is cut into cubes, and the side length of the cubes is 1.0-1.8 cm. Further, in the step S1, the side length of the polyurethane cu